##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920911_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4235722
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.659936133674496	31.0	30.0	34.0	25.0	34.0
2	29.675882411546368	31.0	30.0	34.0	25.0	34.0
3	29.83970288890536	31.0	30.0	34.0	25.0	34.0
4	33.240202968938945	37.0	35.0	37.0	27.0	37.0
5	33.328119503593484	37.0	35.0	37.0	27.0	37.0
6	33.28749077489032	37.0	35.0	37.0	27.0	37.0
7	33.204520032240076	37.0	35.0	37.0	26.0	37.0
8	33.1356946938444	37.0	35.0	37.0	26.0	37.0
9	34.559124512892964	39.0	35.0	39.0	26.0	39.0
10-11	34.55416892326739	39.0	35.0	39.0	26.5	39.0
12-13	34.49445903201391	39.0	35.0	39.0	26.0	39.0
14-15	35.628961839327516	40.0	36.0	41.0	26.0	41.0
16-17	35.60596564174892	40.0	36.0	41.0	26.0	41.0
18-19	35.5738530526791	40.0	36.0	41.0	25.5	41.0
20-21	35.600765819853144	40.0	36.0	41.0	25.5	41.0
22-23	35.50913480629748	40.0	36.0	41.0	25.0	41.0
24-25	35.51497548705982	40.0	36.0	41.0	25.0	41.0
26-27	35.4476809384563	40.0	35.0	41.0	25.0	41.0
28-29	35.336097718405505	40.0	35.0	41.0	25.0	41.0
30-31	35.18972113845054	40.0	35.0	41.0	24.5	41.0
32-33	35.06903840714759	40.0	35.0	41.0	24.0	41.0
34-35	35.002798578376954	40.0	35.0	41.0	24.0	41.0
36-37	34.86083069191038	39.0	35.0	41.0	23.5	41.0
38-39	34.72227013481999	39.0	34.0	41.0	23.0	41.0
40-41	34.592361821668185	39.0	34.0	41.0	22.0	41.0
42-43	34.38998640609559	39.0	34.0	41.0	20.5	41.0
44-45	34.21940616027209	39.0	33.0	41.0	19.0	41.0
46-47	34.013495101897625	38.5	33.0	41.0	18.0	41.0
48-49	33.885034357778906	38.0	33.0	40.0	17.0	41.0
50-51	33.68116439180853	38.0	33.0	40.0	15.0	41.0
52-53	33.481368701723106	38.0	33.0	40.0	13.5	41.0
54-55	33.2356849670493	38.0	32.5	40.0	10.5	41.0
56-57	32.960648267284775	38.0	32.0	40.0	8.5	41.0
58-59	32.68832633492	37.0	32.0	40.0	8.0	41.0
60-61	32.41379356341139	37.0	31.0	40.0	7.0	41.0
62-63	32.095472035228	36.0	31.0	39.5	5.5	41.0
64-65	31.800172320090883	36.0	31.0	39.0	2.0	41.0
66-67	31.44780748122752	35.0	31.0	39.0	2.0	40.5
68-69	31.116585200822904	35.0	30.0	38.5	2.0	40.0
70-71	30.770853587652823	35.0	30.0	37.5	2.0	40.0
72-73	30.834437670838643	35.0	30.5	37.0	2.0	39.5
74-75	30.831248486090445	35.0	31.0	37.0	2.0	39.0
76-77	30.571246649331563	35.0	31.0	36.5	2.0	39.0
78-79	30.284376193716206	35.0	31.0	36.0	2.0	38.5
80-81	30.020053960104086	35.0	31.0	36.0	2.0	37.0
82-83	29.789398950167175	35.0	30.0	35.0	2.0	37.0
84-85	29.56868463038887	35.0	30.0	35.0	2.0	36.5
86-87	29.378121250639204	35.0	30.0	35.0	2.0	36.0
88-89	29.1917550301932	34.5	30.0	35.0	2.0	36.0
90-91	29.030137010880317	34.0	30.0	35.0	2.0	35.5
92-93	28.873507869496628	34.0	30.0	35.0	2.0	35.0
94-95	28.76565506423698	34.0	29.0	35.0	2.0	35.0
96-97	28.669101985446638	34.0	29.0	35.0	2.0	35.0
98-99	28.534970661436233	34.0	29.0	35.0	2.0	35.0
100-101	28.413041035270965	34.0	29.0	35.0	2.0	35.0
102-103	27.037624282235708	32.5	26.0	34.5	2.0	35.0
104-105	28.05846146182398	34.0	29.0	35.0	2.0	35.0
106-107	28.204255850596425	34.0	29.0	35.0	2.0	35.0
108-109	28.13013283213582	34.0	29.0	35.0	2.0	35.0
110-111	28.019449930850044	34.0	29.0	35.0	2.0	35.0
112-113	27.89457547025041	34.0	29.0	35.0	2.0	35.0
114-115	27.775612752678292	34.0	29.0	35.0	2.0	35.0
116-117	27.55386260004788	34.0	27.5	35.0	2.0	35.0
118-119	27.33794510121297	34.0	27.0	35.0	2.0	35.0
120-121	27.14772333972815	34.0	27.0	35.0	2.0	35.0
122-123	26.84654009399106	34.0	26.5	35.0	2.0	35.0
124-125	25.55828758355718	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	175144.0
3	25319.0
4	16723.0
5	15204.0
6	18023.0
7	19928.0
8	20234.0
9	21504.0
10	23078.0
11	24290.0
12	25149.0
13	24599.0
14	24607.0
15	22486.0
16	18736.0
17	17149.0
18	16523.0
19	16609.0
20	17022.0
21	18708.0
22	22386.0
23	28566.0
24	38004.0
25	48712.0
26	55939.0
27	62136.0
28	72105.0
29	84887.0
30	100411.0
31	122959.0
32	151866.0
33	195355.0
34	260227.0
35	380897.0
36	672795.0
37	1037878.0
38	339132.0
39	432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.2558918394562	17.545014275400174	11.404830389610943	25.79426349553269
2	16.473210506877837	20.18767753775847	37.9998307081271	25.339281247236595
3	19.28848065856702	24.11721690728164	29.046844003734805	27.547458430416533
4	12.988202537954527	16.01309762525745	36.06970520412846	34.92899463265956
5	14.018755022568746	37.32865324918516	34.4567017326118	14.195889995634298
6	33.205263499979324	35.829828469460715	17.056032521670282	13.90887550888969
7	28.555816477403788	31.857210518553813	21.29628054014604	18.290692463896356
8	26.50618353786795	35.001709739417755	19.895246262736496	18.5968604599778
9	26.600371227987583	15.506331058415707	18.870236108373618	39.02306160522309
10-11	25.40512663585715	25.826011494789302	27.73602037374311	21.03284149561044
12-13	25.875555561856117	23.395111126547487	27.422457611200386	23.306875700396017
14-15	23.246442796193442	24.74307623334808	24.605672978836637	27.404807991621837
16-17	23.568459426487127	27.42546030269161	25.421389147374	23.584691123447254
18-19	23.576308005520534	26.787992752850197	26.610854115375314	23.024845126253954
20-21	24.36777332248944	26.127448808973263	26.296556827960703	23.208221040576593
22-23	24.085082378020434	25.95510029368076	26.015811326174624	23.944006002124176
24-25	23.715709241559242	26.12638439006044	26.29111208676777	23.866794281612545
26-27	23.705193025563485	26.225676888909888	26.222570051504984	23.846560034021643
28-29	23.733254488307438	26.300222359983138	26.272415549511713	23.694107602197708
30-31	23.887972842989498	26.230085451549208	26.152230409345535	23.729711296115767
32-33	23.592600568865322	26.305750520294286	26.14924581103592	23.952403099804474
34-35	23.873443172166287	26.25945496407025	26.233784950733185	23.633316913030278
36-37	23.91139677755773	26.128462008457532	26.127599622156666	23.832541591828075
38-39	23.792901936523865	26.307962495937844	26.13504529006158	23.76409027747671
40-41	23.90245083793471	26.201898200324635	26.236322868895645	23.65932809284501
42-43	23.67805910607388	26.235979871192548	26.171667109870274	23.914293912863297
44-45	23.74700105959608	26.263890217813735	26.210307967997124	23.77880075459306
46-47	23.7433496815407	26.270104515129177	26.150788588384266	23.83575721494585
48-49	23.59626393235789	26.339340658094628	26.09613341708359	23.968261992463894
50-51	23.599614880286758	26.494662907445942	26.090159638523264	23.815562573744035
52-53	23.7403618481863	26.266625165889906	26.07346936846961	23.919543617454188
54-55	23.559219024365177	26.304221868239154	26.058567856959343	24.077991250436327
56-57	23.63565034525888	26.303796158455956	26.075923169119086	23.984630327166077
58-59	23.609634849134657	26.17406873837979	26.114645543774202	24.101650868711356
60-61	23.659980582347657	26.140769356256037	26.25073341859712	23.948516642799184
62-63	23.514849526210643	26.19128306960648	26.25610789080372	24.037759513379157
64-65	23.43396396677316	26.1394206374179	26.209060560275383	24.21755483553356
66-67	23.51039944587946	26.172757756057504	26.182419902225696	24.13442289583734
68-69	23.596991313117687	26.119543057776433	26.12876846276994	24.154697166335943
70-71	23.426824982946844	25.990580161643244	26.363629383989817	24.21896547142009
72-73	23.43431728294128	26.00775090337048	26.30796494376863	24.24996686991961
74-75	23.339639124188018	26.117844742420885	26.307798030117553	24.234718103273547
76-77	23.45708558878674	25.86621932457361	26.387552732248697	24.289142354390954
78-79	23.345687398794173	25.916488218398236	26.485668209182595	24.252156173624996
80-81	23.382317697102977	25.99136020285912	26.464372146511096	24.161949953526808
82-83	23.357255546215985	25.943611521784426	26.522590024867192	24.176542907132394
84-85	23.358311680012246	25.927580552776003	26.36609648059163	24.34801128662012
86-87	23.345281588768742	25.830071452118762	26.55462071720479	24.2700262419077
88-89	23.38516234955057	25.73846898881448	26.478249058030073	24.398119603604883
90-91	23.3017463310747	25.826269322061624	26.536353794899263	24.335630551964407
92-93	23.284116379715083	25.76052730948593	26.623679343371847	24.33167696742714
94-95	23.25356355815281	25.71056177779122	26.629340340120883	24.40653432393509
96-97	23.351939629947253	25.58408106347026	26.602382209103343	24.461597097479142
98-99	23.284819969934357	25.6545560026872	26.54144277276631	24.51918125461213
100-101	23.255223425626145	25.65840018207155	26.670505885141623	24.41587050716068
102-103	23.126024989246694	25.61905630245412	26.71858646379979	24.536332244499395
104-105	23.012092644452256	25.827454242771246	26.590224317184614	24.570228795591888
106-107	23.002917335545085	25.768250001446912	26.63540699718779	24.593425665820213
108-109	23.005162583342155	25.78565570052764	26.618026000483802	24.5911557156464
110-111	22.907092051556006	26.00491746444052	26.647637036078926	24.440353447924547
112-113	23.001070785855234	25.975020509122356	26.650389304190004	24.373519400832414
114-115	22.98667057272594	26.117030575938987	26.566904652778106	24.329394198556965
116-117	22.91447632971271	26.104579542721435	26.603492033040638	24.377452094525218
118-119	23.0169490484351	26.072438849570105	26.494216052173886	24.416396049820904
120-121	22.98810520977802	26.170286732047042	26.581939059768555	24.25966899840638
122-123	22.989351460852	26.246564177676458	26.48743496004583	24.27664940142571
124-125	23.114166388164232	26.29704461366839	26.289755705814954	24.299033292352423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1884.0
1	1476.0
2	851.0
3	572.0
4	470.5
5	420.0
6	398.5
7	420.0
8	494.5
9	559.0
10	588.5
11	602.5
12	660.0
13	781.0
14	898.0
15	999.5
16	1138.0
17	1343.0
18	1609.0
19	1922.0
20	2424.5
21	3110.5
22	3904.0
23	5018.5
24	6611.5
25	8643.5
26	11471.0
27	15216.0
28	19258.0
29	24268.5
30	30141.5
31	36625.0
32	44067.0
33	50818.5
34	57226.0
35	64353.0
36	72443.5
37	81671.0
38	90608.0
39	98187.5
40	105988.0
41	114416.0
42	122812.5
43	133526.0
44	144595.0
45	154430.5
46	164438.0
47	171850.5
48	178195.5
49	185120.5
50	189380.0
51	187558.0
52	182084.5
53	178085.5
54	172528.0
55	164024.0
56	152125.5
57	136669.0
58	121987.5
59	107450.0
60	92024.5
61	76898.0
62	62361.5
63	47978.5
64	35175.5
65	26020.0
66	19725.0
67	15229.5
68	11556.5
69	8805.0
70	7017.0
71	5388.0
72	4010.5
73	3295.5
74	2658.0
75	2011.5
76	1474.5
77	995.5
78	690.5
79	483.5
80	352.5
81	254.0
82	183.0
83	134.0
84	97.0
85	65.5
86	61.0
87	54.0
88	39.0
89	31.0
90	26.5
91	20.0
92	17.0
93	17.0
94	14.0
95	9.5
96	10.0
97	13.0
98	12.0
99	10.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16807052020883334
2	0.14967932267509532
3	0.17446848494778458
4	0.14762536351535818
5	0.17253256941791742
6	0.11089018589983007
7	0.10789187770113337
8	0.09639442815180034
9	0.1029340452465955
10-11	0.08981939796804417
12-13	0.07779075208429637
14-15	0.07766090409143943
16-17	0.0770234684901417
18-19	0.07463898716676873
20-21	0.06904371911093315
22-23	0.06029668613756993
24-25	0.0631651463434097
26-27	0.07370644249079614
28-29	0.06955130671937393
30-31	0.06741471701872787
32-33	0.07699985976416772
34-35	0.07453274789988577
36-37	0.07743662119468653
38-39	0.07225450584339577
40-41	0.0777199259063744
42-43	0.07744842555767352
44-45	0.07028317722456762
46-47	0.06709599921807899
48-49	0.059045423660948476
50-51	0.06498301824340691
52-53	0.06774523918236372
54-55	0.06939785000054301
56-57	0.07321065924534235
58-59	0.05964744617328522
60-61	0.07024776413560663
62-63	0.06552601894080867
64-65	0.0594467720025063
66-67	0.06393242993756437
68-69	0.06717862975898796
70-71	0.06219718857847611
72-73	0.05687342087134142
74-75	0.060816078108997716
76-77	0.0712511349895012
78-79	0.06380258194470742
80-81	0.062244406030424096
82-83	0.06695434686223506
84-85	0.06899650165898517
86-87	0.06155975297717839
88-89	0.060721643205101754
90-91	0.06384979939665539
92-93	0.058042052807053914
94-95	0.06785147844924667
96-97	0.05426465665121555
98-99	0.05472502680770834
100-101	0.05244678475121833
102-103	0.05054628231031215
104-105	0.048964497670054834
106-107	0.06087509992393268
108-109	0.06067442575315377
110-111	0.06727306466288392
112-113	0.06727306466288392
114-115	0.07040122085443756
116-117	0.07356479013495221
118-119	0.06610443272717142
120-121	0.06852432713950538
122-123	0.06485317025054997
124-125	0.0770470772161157
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4235722.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.308277803315924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.66377863847731	35.54973006376538
2	13.90400716653762	13.989733102269403
3	5.90810542525849	8.916798270755462
4	3.099790350744506	6.237804563891709
5	1.7453933941030275	4.39038678733038
6	1.1105205353027259	3.352102535777799
7	0.7562121679758573	2.663061227734407
8	0.5375605760230616	2.163499743574297
9	0.38869799271260563	1.7599253939079331
>10	1.772916378668192	15.378490081582036
>50	0.0844436607857605	2.871796302425508
>100	0.02758480495402175	2.2494246458030234
>500	7.06363262089023E-4	0.23688241735442409
>1k	2.825451946097078E-4	0.24036486382827382
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.721745194797959E-5
2	0.0	0.0	0.0	0.0	4.721745194797959E-5
3	0.0	0.0	0.0	0.0	4.721745194797959E-5
4	0.0	0.0	0.0	0.0	4.721745194797959E-5
5	0.0	0.0	0.0	0.0	7.082617792196938E-5
6	0.0	0.0	0.0	0.0	1.1804362986994897E-4
7	0.0	0.0	0.0	0.0	1.1804362986994897E-4
8	0.0	0.0	0.0	0.0	1.1804362986994897E-4
9	0.0	0.0	0.0	0.0	1.4165235584393877E-4
10-11	0.0	0.0	0.0	0.0	1.6526108181792856E-4
12-13	0.0	0.0	0.0	0.0	2.5969598571388774E-4
14-15	0.0	0.0	0.0	0.0	3.895439785708316E-4
16-17	0.0	0.0	0.0	1.1804362986994897E-5	4.60370156492801E-4
18-19	0.0	0.0	0.0	2.3608725973989794E-5	4.721745194797959E-4
20-21	0.0	0.0	0.0	5.9021814934974486E-5	5.666094233757551E-4
22-23	0.0	0.0	0.0	1.0623926688295407E-4	5.666094233757551E-4
24-25	0.0	0.0	0.0	3.305221636358571E-4	5.902181493497449E-4
26-27	0.0	0.0	0.0	8.499141350636326E-4	6.610443272717142E-4
28-29	0.0	0.0	0.0	0.0030455256506446834	6.610443272717142E-4
30-31	0.0	0.0	0.0	0.012040450246734795	6.610443272717142E-4
32-33	0.0	0.0	0.0	0.034693022818778	7.554792311676734E-4
34-35	0.0	0.0	0.0	0.07211285348755182	7.790879571416633E-4
36-37	0.0	0.0	0.0	0.13612791396602517	8.026966831156531E-4
38-39	0.0	0.0	0.0	0.24373648695547062	8.499141350636326E-4
40-41	0.0	0.0	0.0	0.36855582117995467	8.971315870116122E-4
42-43	0.0	0.0	0.0	0.490494890835612	8.971315870116122E-4
44-45	0.0	0.0	0.0	0.6365384697107128	8.971315870116122E-4
46-47	0.0	0.0	0.0	0.816661244529268	9.089359499986072E-4
48-49	0.0	0.0	0.0	1.0094973182848166	9.325446759725969E-4
50-51	0.0	0.0	0.0	1.2123907093052848	9.679577649335816E-4
52-53	0.0	0.0	0.0	1.4117309870666677	0.0010151752168815612
54-55	0.0	0.0	0.0	1.6382094953351518	0.0010151752168815612
56-57	0.0	0.0	0.0	1.901434985582151	0.0010505883058425458
58-59	0.0	0.0	0.0	2.191775097610278	0.0010860013948035306
60-61	0.0	0.0	0.0	2.502654801235775	0.0010860013948035306
62-63	0.0	0.0	0.0	2.815128093864517	0.0011096101207775204
64-65	0.0	0.0	0.0	3.1431949499990792	0.0011096101207775204
66-67	0.0	0.0	0.0	3.5179008442952586	0.0011332188467515101
68-69	0.0	0.0	0.0	3.940083886525131	0.0011332188467515101
70-71	0.0	0.0	0.0	4.403027394149097	0.0011686319357124947
72-73	0.0	0.0	0.0	4.867977643480852	0.0012276537506474693
74-75	0.0	0.0	0.0	5.337685051096365	0.0012866755655824439
76-77	0.0	0.0	0.0	5.852756625670901	0.0013456973805174182
78-79	0.0	0.0	0.0	6.422529618327171	0.001381110469478403
80-81	0.0	0.0	0.0	7.030891073587927	0.0014047191954523928
82-83	0.0	0.0	0.0	7.666107454644097	0.0014755453733743622
84-85	0.0	0.0	0.0	8.298467179857413	0.0015109584623353468
86-87	0.0	0.0	0.0	8.96358401235964	0.0015699802772703214
88-89	0.0	0.0	0.0	9.684889612679964	0.001605393366231306
90-91	0.0	0.0	0.0	10.454770166691771	0.001605393366231306
92-93	2.3608725973989794E-5	0.0	0.0	11.240232479846412	0.0016526108181792857
94-95	2.3608725973989794E-5	0.0	0.0	12.026166967520531	0.00171163263311426
96-97	2.3608725973989794E-5	0.0	0.0	12.82963093423034	0.0018296762629842092
98-99	2.3608725973989794E-5	0.0	0.0	13.685494468239417	0.0019123068038931733
100-101	2.3608725973989794E-5	0.0	0.0	14.601406796763339	0.0019123068038931733
102-103	2.3608725973989794E-5	0.0	0.0	15.542072874470987	0.0019241111668801682
104-105	2.3608725973989794E-5	0.0	0.0	16.472528178194885	0.001947719892854158
106-107	2.3608725973989794E-5	0.0	0.0	17.392831729749968	0.0019831329818151425
108-109	2.3608725973989794E-5	0.0	0.0	18.34610486712773	0.0020303504337631224
110-111	2.3608725973989794E-5	1.1804362986994897E-5	0.0	19.345072693628147	0.002053959159737112
112-113	2.3608725973989794E-5	7.082617792196938E-5	0.0	20.384682941892788	0.002065763522724107
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8445	0.0	32.88438	1
GTACATG	14430	0.0	30.80888	1
TCAACGC	9025	0.0	29.502806	4
TACATGG	15055	0.0	29.241009	2
CATGGGG	10460	0.0	28.47353	4
CAACGCA	9390	0.0	28.355997	5
ACATGGG	15240	0.0	28.065203	3
AACGCAG	9550	0.0	27.674166	6
GGTATCA	6205	0.0	27.660124	1
ATCAACG	9795	0.0	27.063519	3
TATCAAC	10255	0.0	26.081907	2
ACGCAGA	10930	0.0	24.0686	7
CGCAGAG	11170	0.0	23.602232	8
GTATAGG	1140	0.0	18.819197	1
GCAGAGT	13250	0.0	18.728685	9
TATACCG	295	2.5555491E-6	18.172869	5
AGAGTAC	11195	0.0	17.832382	10-11
ATGGGGC	4450	0.0	17.401487	5
ATGGGGG	5875	0.0	17.33769	5
ATGGGGA	5935	0.0	17.06205	5
>>END_MODULE