FastQCFastQC Report
Mon 15 Aug 2016
SRR2920911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4235722
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64420.15208741272444226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52660.12432355097903025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74750.042.6343231
GTATCAA110850.041.455711
TCAACGC131400.034.2686964
CAACGCA135400.033.3886955
ATCAACG137850.032.9245153
AACGCAG139650.032.6072776
TATCAAC139700.032.531912
GTACATG145200.029.5112191
TACATGG151250.027.8423212
ACGCAGA161200.027.8331177
ACATGGG153450.026.7438223
CGCAGAG167000.026.7182528
CATGGGG102550.026.5431044
GCAGAGT187400.023.3995389
GAGTACT102700.022.33515412-13
AGAGTAC165200.021.26801310-11
CAGAGTA175600.020.95715110-11
AGTACTT109850.020.66471912-13
GTACTTT113350.020.28910814-15
ATGGGGC43150.017.3926625
Produced by FastQC (version 0.11.5)