FastQCFastQC Report
Mon 15 Aug 2016
SRR2920910_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920910_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3276888
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG117300.029.5143011
TACATGG114950.029.0153332
CATGGGG75100.027.9133174
ACATGGG120650.027.2496093
GTATCAA100650.024.154651
AACGCAG107650.021.5079366
TCAACGC108150.021.4206094
CAACGCA108950.021.263325
ATCAACG110800.021.0714723
TATCAAC113550.020.9807682
GGTATCA80800.019.7641351
ACGCAGA120500.019.2137647
GTACTAT8100.019.1268181
CGCAGAG123100.018.5155898
ATGGGGA43300.018.1549385
GAGTACT38100.018.0387912-13
GTATAAT14700.017.8356821
AGAGTAC95900.017.09516310-11
ATGGGGC35950.017.0626725
ATGGGGG36050.016.8501455