##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920910_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3276888
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.770806631169574	31.0	30.0	34.0	25.0	34.0
2	29.792116178520597	31.0	30.0	34.0	25.0	34.0
3	29.953033793037786	31.0	31.0	34.0	25.0	34.0
4	33.339595067027005	37.0	35.0	37.0	27.0	37.0
5	33.448291488753966	37.0	35.0	37.0	27.0	37.0
6	33.4085998667028	37.0	35.0	37.0	27.0	37.0
7	33.33431871946798	37.0	35.0	37.0	27.0	37.0
8	33.26131775025573	37.0	35.0	37.0	26.0	37.0
9	34.68209136229252	39.0	35.0	39.0	27.0	39.0
10-11	34.689087024030115	39.0	35.0	39.0	27.0	39.0
12-13	34.638529299750246	39.0	35.0	39.0	27.0	39.0
14-15	35.78847491888646	40.0	36.0	41.0	27.0	41.0
16-17	35.77537743127016	40.0	36.0	41.0	27.0	41.0
18-19	35.75810387782555	40.0	36.0	41.0	27.0	41.0
20-21	35.796460696856286	40.0	36.0	41.0	27.0	41.0
22-23	35.71056441355334	40.0	36.0	41.0	26.0	41.0
24-25	35.7206712588285	40.0	36.0	41.0	27.0	41.0
26-27	35.65517375021667	40.0	36.0	41.0	26.5	41.0
28-29	35.54088436956039	40.0	35.0	41.0	26.0	41.0
30-31	35.3882503765768	40.0	35.0	41.0	25.0	41.0
32-33	35.26062440950072	40.0	35.0	41.0	25.0	41.0
34-35	35.18720551327967	40.0	35.0	41.0	24.5	41.0
36-37	35.03711158269675	40.0	35.0	41.0	24.0	41.0
38-39	34.89583348591712	39.0	35.0	41.0	24.0	41.0
40-41	34.76702728320284	39.0	34.0	41.0	23.0	41.0
42-43	34.561462430208174	39.0	34.0	41.0	22.0	41.0
44-45	34.39326595843373	39.0	34.0	41.0	21.5	41.0
46-47	34.18828809529041	39.0	33.0	41.0	19.5	41.0
48-49	34.05683074917422	39.0	33.0	41.0	18.5	41.0
50-51	33.85691164910122	38.0	33.0	40.0	18.0	41.0
52-53	33.660836592523154	38.0	33.0	40.0	15.5	41.0
54-55	33.420535123568456	38.0	33.0	40.0	13.5	41.0
56-57	33.14917842782542	38.0	32.0	40.0	11.5	41.0
58-59	32.88194134190732	37.0	32.0	40.0	8.5	41.0
60-61	32.61361892747021	37.0	32.0	40.0	8.0	41.0
62-63	32.30794110143526	36.5	31.0	40.0	7.0	41.0
64-65	32.02181795654902	36.0	31.0	39.0	6.5	41.0
66-67	31.668543142151943	35.5	31.0	39.0	2.0	41.0
68-69	31.33268271604034	35.0	30.5	39.0	2.0	40.0
70-71	30.985773850067503	35.0	30.0	38.0	2.0	40.0
72-73	31.0477871077681	35.0	31.0	37.5	2.0	40.0
74-75	31.04064893276792	35.0	31.0	37.0	2.0	39.0
76-77	30.77351407799107	35.0	31.0	37.0	2.0	39.0
78-79	30.475882758275535	35.0	31.0	36.0	2.0	39.0
80-81	30.194406095051157	35.0	31.0	36.0	2.0	37.5
82-83	29.945991593243345	35.0	31.0	35.5	2.0	37.0
84-85	29.70778159033815	35.0	30.0	35.0	2.0	37.0
86-87	29.50770532895845	35.0	30.0	35.0	2.0	36.0
88-89	29.30582018671374	35.0	30.0	35.0	2.0	36.0
90-91	29.13151944771991	34.5	30.0	35.0	2.0	36.0
92-93	28.963503787740073	34.0	30.0	35.0	2.0	35.0
94-95	28.84548022391977	34.0	30.0	35.0	2.0	35.0
96-97	28.744078528164525	34.0	29.5	35.0	2.0	35.0
98-99	28.60843779219796	34.0	29.0	35.0	2.0	35.0
100-101	28.483668651476645	34.0	29.0	35.0	2.0	35.0
102-103	27.103228734091612	32.5	26.0	34.5	2.0	35.0
104-105	28.120128152075992	34.0	29.0	35.0	2.0	35.0
106-107	28.266361254946766	34.0	29.0	35.0	2.0	35.0
108-109	28.19634451955636	34.0	29.0	35.0	2.0	35.0
110-111	28.089281507332565	34.0	29.0	35.0	2.0	35.0
112-113	27.958672984856364	34.0	29.0	35.0	2.0	35.0
114-115	27.83443849774542	34.0	29.0	35.0	2.0	35.0
116-117	27.615991758033843	34.0	28.5	35.0	2.0	35.0
118-119	27.41240606941708	34.0	27.0	35.0	2.0	35.0
120-121	27.2275743022038	34.0	27.0	35.0	2.0	35.0
122-123	26.92396902182803	34.0	27.0	35.0	2.0	35.0
124-125	25.626286281374277	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	124503.0
3	17710.0
4	12117.0
5	11633.0
6	15299.0
7	16228.0
8	15334.0
9	16245.0
10	17783.0
11	19156.0
12	19527.0
13	19427.0
14	19742.0
15	18134.0
16	15062.0
17	14003.0
18	13291.0
19	13102.0
20	13524.0
21	14664.0
22	17781.0
23	22354.0
24	29239.0
25	37706.0
26	42856.0
27	46788.0
28	54672.0
29	63971.0
30	76152.0
31	92402.0
32	114832.0
33	147538.0
34	198884.0
35	289917.0
36	510636.0
37	812666.0
38	291596.0
39	414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.255901489914905	18.40320932965139	11.866315427169871	25.47457375326383
2	16.11636409422791	20.689072399483024	38.505563231542716	24.68900027474635
3	19.168509471210367	24.39132425758464	29.372032126854243	27.068134144350747
4	12.73511127464493	16.437389943199843	36.60829161285433	34.219207169300894
5	13.571849321705576	37.862581605793544	34.63671303158588	13.928856040915004
6	32.54755558271151	36.51715789232497	17.104429050415867	13.830857474547662
7	27.88193138641934	32.60787146493908	21.44326382076652	18.066933327875056
8	25.824961809232803	35.67220790439677	20.059186907035073	18.44364337933535
9	26.05957703676459	16.174702365314424	19.423756276203033	38.34196432171795
10-11	24.88392358887096	26.400988295074928	28.019170285416173	20.695917830637942
12-13	25.342138174621713	24.07565200262038	27.6105224419615	22.971687380796407
14-15	22.80782043670427	25.492281812784483	25.01143740547725	26.688460345034
16-17	22.946338981441	28.05051030871398	25.87775282449922	23.1253978853458
18-19	22.911059773923977	27.453866391076676	27.179620144516775	22.45545369048257
20-21	23.916103904511726	26.656969873163987	26.761836938736742	22.66508928358754
22-23	23.39564924053395	26.6205878989569	26.48974418909649	23.494018671412665
24-25	23.08522899994702	26.702198916779384	26.790678372521565	23.42189371075203
26-27	23.139162974163114	26.72881392683361	26.81383643611539	23.31818666288789
28-29	23.086257221541217	27.016412496511	26.804247753450976	23.093082528496804
30-31	23.410227004310507	26.888451627314907	26.587536131464713	23.113785236909877
32-33	22.957750307158605	27.008777574413617	26.66495539443196	23.368516723995814
34-35	23.23371554838492	26.938854600850128	26.736119223122063	23.091310627642894
36-37	23.335781123854993	26.726390922988053	26.73524755743947	23.202580395717483
38-39	23.16595371719866	27.03621956349627	26.67384833148447	23.123978387820586
40-41	23.25566515905232	26.920421692238694	26.73589812539998	23.088015023309
42-43	23.1271876365722	26.917004111023594	26.636507147793758	23.31930110461045
44-45	23.216666852438888	26.920562831803817	26.64671422130113	23.216056094456164
46-47	23.056527822650338	26.942250693650056	26.590600805079905	23.410620678619697
48-49	22.98691495868195	26.93093139124405	26.701127127926107	23.381026522147895
50-51	22.891459387162133	27.14084827460902	26.595358750933272	23.372333587295575
52-53	23.010873261225623	27.03791769863631	26.465221306052538	23.48598773408553
54-55	23.028028712757	26.891428131685846	26.47709276311731	23.603450392439846
56-57	22.988235811482255	26.909520008159987	26.407109574123062	23.695134606234696
58-59	22.969284839675964	26.789687107816047	26.60238539449698	23.638642658011012
60-61	23.028030345135917	26.695446824858287	26.682834574301424	23.59368825570437
62-63	22.833058121275	26.740122711751056	26.644222188122903	23.782596978851043
64-65	22.80668192897454	26.73866262182429	26.638081010335263	23.816574438865906
66-67	22.877940659824205	26.78225959994766	26.473492322347358	23.866307417880776
68-69	22.931466372939926	26.557249994579657	26.568823577373063	23.942460055107354
70-71	22.78948441189336	26.328651443080258	26.894310507114444	23.987553637911937
72-73	22.838488649297297	26.25288261818448	26.907153931238536	24.001474801279688
74-75	22.621242700368573	26.48759499692892	26.868002571674904	24.023159731027597
76-77	22.641191952527848	26.25918301004384	27.043448293658436	24.056176743769875
78-79	22.676255209842644	26.307655617377613	26.991175979316058	24.024913193463686
80-81	22.717120351134387	26.237212666206904	27.005456549974554	24.04021043268415
82-83	22.69987481366719	26.150003427775548	27.142959005483984	24.00716275307328
84-85	22.745728427152397	26.216366097123622	26.914573429033506	24.123332046690475
86-87	22.532543276527008	26.084424725256273	27.114404541248966	24.268627456967756
88-89	22.60845681384478	26.012902035918856	27.082107154411446	24.296533995824923
90-91	22.6064659787065	25.979280579406023	27.09954678175963	24.31470666012785
92-93	22.704092265164945	25.96449369167072	27.097747157209835	24.2336668859545
94-95	22.480521086696623	25.937010927735255	27.186989933413646	24.395478052154477
96-97	22.46340680723916	25.87363681025278	27.191645158621135	24.47131122388692
98-99	22.42206829926909	25.922935966935352	27.099397754521576	24.555597979273987
100-101	22.300770148619385	25.986210968938806	27.2210623312323	24.49195655120951
102-103	22.249875468795295	25.977216934045554	27.242573047493444	24.5303345496657
104-105	22.114009686614644	26.04455806099175	27.130260648917996	24.711171603475606
106-107	22.003265671290873	26.108026696366615	27.25661474010127	24.632092892241246
108-109	21.945705433334545	26.169355786755794	27.229543201240187	24.655395578669474
110-111	21.932187277797212	26.3802357408887	27.14467822555046	24.542898755763627
112-113	21.938176925623225	26.39866407388452	27.08270478317004	24.580454217322213
114-115	21.842160552414246	26.457043880189723	27.098151738020988	24.60264382937504
116-117	21.75202639520616	26.425729584728696	27.159849309856	24.66239471020915
118-119	21.779901141541284	26.26877872518576	27.172808091406363	24.77851204186659
120-121	21.72728080566007	26.42128467956605	27.15395554627235	24.697478968501535
122-123	21.697682098830935	26.45434698268853	27.159047689862675	24.688923228617856
124-125	21.741314689842255	26.541840584103	26.896559622345617	24.82028510370913
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1748.0
1	1308.0
2	698.0
3	471.5
4	381.5
5	352.0
6	359.0
7	392.0
8	454.0
9	516.5
10	509.0
11	532.0
12	609.5
13	709.0
14	827.0
15	943.0
16	1122.0
17	1348.5
18	1634.0
19	1931.0
20	2416.5
21	3134.5
22	4075.0
23	5230.5
24	6729.5
25	8716.5
26	11791.5
27	15894.5
28	19615.0
29	23720.5
30	28893.5
31	34688.5
32	41023.5
33	47356.5
34	54263.5
35	60639.0
36	67645.5
37	75547.0
38	80895.0
39	85704.5
40	92055.5
41	97837.5
42	103086.0
43	110426.5
44	117229.5
45	121112.0
46	125296.0
47	129859.0
48	134643.0
49	137164.5
50	137482.5
51	134880.0
52	128223.5
53	123344.0
54	118791.0
55	112238.0
56	104657.0
57	95504.5
58	86693.5
59	77280.5
60	65016.5
61	52776.5
62	42002.5
63	32124.5
64	23930.5
65	18226.0
66	13954.0
67	10527.5
68	8069.5
69	6243.0
70	5043.0
71	3984.5
72	3039.0
73	2502.5
74	2037.0
75	1589.5
76	1156.0
77	787.5
78	545.5
79	383.0
80	280.5
81	212.0
82	138.5
83	91.0
84	78.0
85	63.5
86	46.5
87	31.0
88	28.0
89	29.5
90	26.0
91	22.0
92	19.0
93	15.0
94	17.0
95	15.0
96	11.5
97	12.0
98	11.5
99	14.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16558393207213673
2	0.14583958926884288
3	0.17107694861710257
4	0.14385599996093856
5	0.16848302413753538
6	0.11025704876089754
7	0.1089753449004055
8	0.09737897663881097
9	0.10412318028568568
10-11	0.09054322271618681
12-13	0.07790928466276541
14-15	0.07763463383551712
16-17	0.07771092573197497
18-19	0.07502545097665834
20-21	0.06915097494940321
22-23	0.060682574442580885
24-25	0.06458871954122325
26-27	0.0751017428731162
28-29	0.07049371232706153
30-31	0.06757936188237132
32-33	0.07693274838810481
34-35	0.07392684766766518
36-37	0.07649025538864923
38-39	0.07157705725676312
40-41	0.07658180566439866
42-43	0.07693274838810481
44-45	0.06922726684586107
46-47	0.06658756722841916
48-49	0.058805793789717564
50-51	0.06478707847201369
52-53	0.06724367753795674
54-55	0.06854063977774034
56-57	0.07244678487638272
58-59	0.05975181330579501
60-61	0.07000544418973123
62-63	0.06532112174721871
64-65	0.05906518623767428
66-67	0.06382580057664468
68-69	0.06680118453850116
70-71	0.062116862095988636
72-73	0.057081596929769954
74-75	0.06040792361533259
76-77	0.0700207025690228
78-79	0.06338330757718909
80-81	0.0615370436829089
82-83	0.06620610774612987
84-85	0.06762513702024604
86-87	0.061582818820783626
88-89	0.06037740685674945
90-91	0.06365795840443739
92-93	0.057752965618599106
94-95	0.06692325157283374
96-97	0.05361794483058316
98-99	0.052748217210963566
100-101	0.05114608738534854
102-103	0.048964139146653776
104-105	0.04710261687308202
106-107	0.0583022672730957
108-109	0.05801235806655583
110-111	0.06556525581588385
112-113	0.06579413150525742
114-115	0.06847960626057406
116-117	0.07183644970471983
118-119	0.0642377768175171
120-121	0.06582464826384057
122-123	0.06388683409381096
124-125	0.07523906828674035
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3276888.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.74700098385656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.80640493935391	35.42465700136883
2	14.291355218790178	14.504868346971774
3	6.120627087855332	9.31810406547641
4	3.225748857058461	6.547883216912795
5	1.9064102004396288	4.837230015867202
6	1.1734934720162196	3.573076462737381
7	0.7715637017993979	2.740818075402544
8	0.5473592885342661	2.222147388301714
9	0.38348497152976013	1.7514641004763452
>10	1.6821041224573543	14.012514026004407
>50	0.06512365054792905	2.225821306783922
>100	0.02517956461625136	2.215632150298467
>500	6.628513177526689E-4	0.233904448213058
>1k	4.8207368349955065E-4	0.39187939518519216
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.051675858314352E-5
2	0.0	6.103351716628704E-5	0.0	0.0	3.051675858314352E-5
3	0.0	6.103351716628704E-5	0.0	0.0	3.051675858314352E-5
4	0.0	9.155027574943056E-5	0.0	0.0	3.051675858314352E-5
5	0.0	9.155027574943056E-5	0.0	0.0	3.051675858314352E-5
6	0.0	9.155027574943056E-5	0.0	0.0	9.155027574943056E-5
7	0.0	9.155027574943056E-5	0.0	0.0	9.155027574943056E-5
8	0.0	9.155027574943056E-5	0.0	0.0	1.2206703433257408E-4
9	0.0	1.525837929157176E-4	0.0	0.0	1.2206703433257408E-4
10-11	0.0	1.525837929157176E-4	0.0	0.0	1.2206703433257408E-4
12-13	0.0	1.525837929157176E-4	0.0	0.0	1.2206703433257408E-4
14-15	0.0	3.6620110299772225E-4	0.0	0.0	1.8310055149886112E-4
16-17	0.0	3.6620110299772225E-4	0.0	1.525837929157176E-5	2.4413406866514816E-4
18-19	0.0	3.6620110299772225E-4	0.0	4.577513787471528E-5	2.7465082724829166E-4
20-21	0.0	3.81459482289294E-4	0.0	7.62918964578588E-5	2.7465082724829166E-4
22-23	0.0	4.42492999455581E-4	0.0	2.593924479567199E-4	3.6620110299772225E-4
24-25	0.0	4.8826813733029626E-4	0.0	6.713686888291574E-4	4.577513787471528E-4
26-27	0.0	5.187848959134398E-4	0.0	0.0012359287226173126	4.577513787471528E-4
28-29	0.0	5.187848959134398E-4	0.0	0.004653805683929387	4.577513787471528E-4
30-31	0.0	5.187848959134398E-4	0.0	0.016967317772227798	4.7300975803872455E-4
32-33	0.0	5.187848959134398E-4	0.0	0.048826813733029634	5.187848959134398E-4
34-35	0.0	6.408519302460139E-4	0.0	0.09991186760121189	5.187848959134398E-4
36-37	0.0	7.62918964578588E-4	0.0	0.18249021632719825	5.187848959134398E-4
38-39	0.0	7.62918964578588E-4	0.0	0.3100960423426129	5.187848959134398E-4
40-41	0.0	7.934357231617314E-4	0.0	0.4521820703057291	5.187848959134398E-4
42-43	0.0	8.23952481744875E-4	0.0	0.5945885242339684	5.493016544965833E-4
44-45	0.0	9.307611367858773E-4	0.0	0.7673896697110185	5.798184130797268E-4
46-47	0.0	0.001007053033243736	0.0	0.9702193056338819	6.103351716628704E-4
48-49	0.0	0.001007053033243736	0.0	1.18542348716221	6.103351716628704E-4
50-51	0.0	0.0012511871019088843	0.0	1.4077991069575768	6.103351716628704E-4
52-53	0.0	0.0012511871019088843	0.0	1.6297474921327797	6.713686888291574E-4
54-55	0.0	0.0012511871019088843	0.0	1.8778487394137366	7.324022059954445E-4
56-57	0.0	0.0012511871019088843	0.0	2.16989411905442	7.324022059954445E-4
58-59	0.0	0.0012511871019088843	0.0	2.4832096794275547	7.62918964578588E-4
60-61	0.0	0.001266445481200456	0.0	2.811112250403432	8.392108610364467E-4
62-63	0.0	0.0013122206190751713	0.0	3.135902112003828	9.155027574943056E-4
64-65	0.0	0.0013427373776583149	0.0	3.48841644877701	9.155027574943056E-4
66-67	0.0	0.00138851251553303	0.0	3.8947776060701496	9.155027574943056E-4
68-69	0.0	0.0014342876534077455	0.0	4.347203810444544	9.155027574943056E-4
70-71	0.0	0.001464804411990889	0.0	4.837806479806451	9.155027574943056E-4
72-73	0.0	0.001464804411990889	0.0	5.3187353366975	0.0011291200675763102
74-75	0.0	0.001464804411990889	0.0	5.808163110853957	0.0011291200675763102
76-77	0.0	0.0014953211705740325	0.0	6.348294479396305	0.0011291200675763102
78-79	0.0	0.001525837929157176	0.0	6.942486285768693	0.0011291200675763102
80-81	0.0	0.001525837929157176	0.0	7.575281791748757	0.0011596368261594537
82-83	0.0	0.001541096308448748	0.0	8.22592960149996	0.0012511871019088843
84-85	0.0	0.0016173882049066064	0.0	8.878118507559611	0.0012511871019088843
86-87	0.0	0.0016326465841981783	0.0	9.565676336817127	0.0012511871019088843
88-89	0.0	0.0017394552392391805	0.0	10.316602215272539	0.001266445481200456
90-91	0.0	0.0017394552392391805	0.0	11.10810012426424	0.0012817038604920278
92-93	0.0	0.001769971997822324	0.0	11.921615874573682	0.0012817038604920278
94-95	0.0	0.0018462638942801828	0.0	12.725366262136514	0.0013732541362414584
96-97	0.0	0.0018920390321548982	0.0	13.558992556352248	0.0013732541362414584
98-99	0.0	0.0020598812043621873	0.0	14.44564171860619	0.0013732541362414584
100-101	0.0	0.002075139583653759	0.0	15.392134244441678	0.001403770894824602
102-103	0.0	0.002075139583653759	0.0	16.36166692300744	0.0014190292741161738
104-105	0.0	0.0021209147215284748	0.0	17.314339092456013	0.0014342876534077455
106-107	3.051675858314352E-5	0.002151431480111618	0.0	18.26461569635581	0.0014342876534077455
108-109	3.051675858314352E-5	0.0021972066179863333	0.0	19.248323409283444	0.0014953211705740325
110-111	3.051675858314352E-5	0.002227723376569477	0.0	20.282444807390426	0.0016021298256150346
112-113	3.051675858314352E-5	0.002288756893735764	0.0	21.350302482111076	0.0016173882049066064
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	11730	0.0	29.514301	1
TACATGG	11495	0.0	29.015333	2
CATGGGG	7510	0.0	27.913317	4
ACATGGG	12065	0.0	27.249609	3
GTATCAA	10065	0.0	24.15465	1
AACGCAG	10765	0.0	21.507936	6
TCAACGC	10815	0.0	21.420609	4
CAACGCA	10895	0.0	21.26332	5
ATCAACG	11080	0.0	21.071472	3
TATCAAC	11355	0.0	20.980768	2
GGTATCA	8080	0.0	19.764135	1
ACGCAGA	12050	0.0	19.213764	7
GTACTAT	810	0.0	19.126818	1
CGCAGAG	12310	0.0	18.515589	8
ATGGGGA	4330	0.0	18.154938	5
GAGTACT	3810	0.0	18.03879	12-13
GTATAAT	1470	0.0	17.835682	1
AGAGTAC	9590	0.0	17.095163	10-11
ATGGGGC	3595	0.0	17.062672	5
ATGGGGG	3605	0.0	16.850145	5
>>END_MODULE