FastQCFastQC Report
Mon 15 Aug 2016
SRR2920910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3276888
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62260.18999733893865156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51700.157771641874852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35370.10793777510857863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA123550.032.886351
GGTATCA90550.030.836761
CAACGCA147050.027.0904225
TCAACGC147100.027.0812134
ATCAACG149400.026.66433
AACGCAG150900.026.464026
TATCAAC151750.026.3326722
GTACATG114250.026.2155421
TACATGG114200.025.5523642
ACATGGG115150.024.408033
ACGCAGA169650.023.3945647
GAGTACT93600.023.33099212-13
AGTACTT98150.022.88598612-13
CGCAGAG171900.022.8782168
CATGGGG70350.022.68434
AGAGTAC146700.022.30876510-11
GTACTTT104500.020.81201214-15
GCAGAGT188650.020.4706139
GTACCGT4657.2759576E-1219.1982146
CAGAGTA177650.018.33841910-11
Produced by FastQC (version 0.11.5)