FastQCFastQC Report
Mon 15 Aug 2016
SRR2920909_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920909_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4787351
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108350.037.4013061
TCAACGC125550.031.4998254
GTACATG181900.031.4845491
GGTATCA76550.030.906661
CAACGCA127750.030.8174935
ATCAACG130000.030.3321513
AACGCAG129600.030.310066
TATCAAC131550.030.2917122
TACATGG188900.029.7980882
ACATGGG183650.029.0282363
CATGGGG131350.028.1137244
ACGCAGA145050.026.873057
CGCAGAG151550.025.5617858
GTATAGG13550.025.5091191
GCAGAGT171500.021.8589329
ATGGGGA73250.020.6529875
ATGGGGG66100.019.7332865
AGAGTAC146000.019.07336810-11
TAAGGTG18850.018.958185
CAGAGTA159400.018.3845210-11
Produced by FastQC (version 0.11.5)