##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920909_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4787351
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.555209342285536	31.0	30.0	34.0	16.0	34.0
2	28.67545183129459	31.0	30.0	34.0	16.0	34.0
3	28.874506172620308	31.0	30.0	34.0	16.0	34.0
4	32.19450422582342	35.0	33.0	37.0	19.0	37.0
5	32.25088091514493	35.0	33.0	37.0	20.0	37.0
6	32.21480208992405	36.0	33.0	37.0	19.0	37.0
7	32.08115218625081	36.0	33.0	37.0	18.0	37.0
8	32.006232674395505	36.0	33.0	37.0	17.0	37.0
9	33.32534088267186	38.0	33.0	39.0	17.0	39.0
10-11	33.30257662327246	38.0	33.0	39.0	17.0	39.0
12-13	33.223179583030365	38.0	33.0	39.0	17.0	39.0
14-15	34.244993630088956	39.0	33.0	41.0	17.0	41.0
16-17	34.205242314591096	39.0	33.0	41.0	16.5	41.0
18-19	34.16480951574263	39.0	33.5	41.0	16.0	41.0
20-21	34.16104052115669	39.0	33.5	41.0	16.0	41.0
22-23	34.06263035653747	39.0	33.0	41.0	16.0	41.0
24-25	34.04545499170627	39.0	33.0	41.0	16.0	41.0
26-27	33.947176737197665	39.0	33.0	41.0	15.5	41.0
28-29	33.81877044319499	39.0	33.0	41.0	11.5	41.0
30-31	33.64929039044766	39.0	33.0	41.0	9.5	41.0
32-33	33.4897521614772	39.0	33.0	41.0	9.0	41.0
34-35	33.41104997314799	38.5	33.0	41.0	8.5	41.0
36-37	33.26074722743329	38.0	32.0	41.0	8.0	41.0
38-39	33.10474414765076	38.0	32.0	41.0	4.5	41.0
40-41	32.94116506184736	38.0	32.0	40.5	2.0	41.0
42-43	32.72661227472145	38.0	31.5	40.0	2.0	41.0
44-45	32.54756367352216	38.0	31.0	40.0	2.0	41.0
46-47	32.30529618571941	38.0	31.0	40.0	2.0	41.0
48-49	32.15347391490617	38.0	31.0	40.0	2.0	41.0
50-51	31.923509055425434	37.5	31.0	40.0	2.0	41.0
52-53	31.695381433281163	37.0	30.0	40.0	2.0	41.0
54-55	31.4258099103241	37.0	30.0	40.0	2.0	41.0
56-57	31.126514746881938	36.0	29.5	40.0	2.0	41.0
58-59	30.832236345319153	36.0	29.0	40.0	2.0	41.0
60-61	30.536553618065607	35.5	29.0	39.0	2.0	41.0
62-63	30.21133681236241	35.0	28.5	39.0	2.0	41.0
64-65	29.90950475534382	35.0	28.0	38.5	2.0	40.0
66-67	29.551843702289638	35.0	28.0	38.0	2.0	40.0
68-69	29.221278636139274	34.5	28.0	37.5	2.0	40.0
70-71	28.888501072931568	34.0	27.0	37.0	2.0	39.0
72-73	28.969306512098235	34.0	28.0	36.5	2.0	39.0
74-75	28.990012221790295	35.0	29.0	36.0	2.0	39.0
76-77	28.75420425617424	34.5	29.0	36.0	2.0	38.5
78-79	28.50102029285089	34.0	28.5	35.0	2.0	37.0
80-81	28.267023245214315	34.0	28.0	35.0	2.0	37.0
82-83	28.05796744379094	34.0	28.0	35.0	2.0	36.5
84-85	27.85776852376189	34.0	27.5	35.0	2.0	36.0
86-87	27.690443107263285	34.0	27.0	35.0	2.0	36.0
88-89	27.514122946071847	34.0	27.0	35.0	2.0	35.5
90-91	27.35813542813134	34.0	27.0	35.0	2.0	35.0
92-93	27.207138770480796	34.0	26.0	35.0	2.0	35.0
94-95	27.107492431618237	34.0	26.0	35.0	2.0	35.0
96-97	27.018369240107944	34.0	25.5	35.0	2.0	35.0
98-99	26.887547831775862	34.0	25.0	35.0	2.0	35.0
100-101	26.765992090406574	34.0	25.0	35.0	2.0	35.0
102-103	25.430464989928666	31.5	22.0	34.0	2.0	35.0
104-105	26.433312702578107	33.5	24.5	35.0	2.0	35.0
106-107	26.593034435954245	34.0	25.0	35.0	2.0	35.0
108-109	26.526475288734837	34.0	25.0	35.0	2.0	35.0
110-111	26.422044466762515	34.0	24.0	35.0	2.0	35.0
112-113	26.30632431171226	34.0	24.0	35.0	2.0	35.0
114-115	26.191047199171315	34.0	24.0	35.0	2.0	35.0
116-117	25.967920672622498	34.0	23.0	35.0	2.0	35.0
118-119	25.76554685461751	34.0	20.0	35.0	2.0	35.0
120-121	25.583353403583736	34.0	19.0	35.0	2.0	35.0
122-123	25.279001477017246	34.0	9.5	35.0	2.0	35.0
124-125	23.97629022814496	31.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	294770.0
3	41192.0
4	26911.0
5	24440.0
6	28884.0
7	30246.0
8	30554.0
9	31951.0
10	34014.0
11	35573.0
12	35487.0
13	34492.0
14	34093.0
15	30389.0
16	24768.0
17	22949.0
18	22203.0
19	22287.0
20	23095.0
21	24938.0
22	29717.0
23	38072.0
24	49492.0
25	61367.0
26	69248.0
27	78695.0
28	91258.0
29	104585.0
30	123511.0
31	148211.0
32	181704.0
33	228524.0
34	303478.0
35	440606.0
36	755834.0
37	978066.0
38	251532.0
39	215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.61492489157911	17.25837577163642	11.746616809899209	25.38008252688526
2	16.637058359604953	19.82456764270876	37.69521713572055	25.84315686196574
3	19.670016346639148	23.67898146962396	28.1256983731202	28.52530381061669
4	13.380239704470617	15.815922128785559	34.59029689122217	36.213541275521656
5	14.68293367822545	37.09911470390006	33.24159320237226	14.976358415502233
6	34.357244815721444	34.70916472294123	16.488333255960725	14.445257205376603
7	29.47056337553985	31.666890068235414	20.119419813927752	18.743126742296983
8	26.961098972327058	34.465160528159075	19.30822870166121	19.265511797852657
9	26.48190862968788	16.080920666206225	18.472144418982833	38.965026285123066
10-11	25.853608716004985	25.850378537228696	26.497219223766088	21.79879352300023
12-13	26.252521390400602	23.285240252522645	26.506369404781488	23.955868952295262
14-15	23.56864883125484	24.64354192414403	23.786330439552536	28.001478805048592
16-17	23.92577124678434	27.316123486448962	24.71494379358887	24.043161473177832
18-19	24.124885706055416	26.327617974136437	26.098423861941694	23.449072457866453
20-21	24.913328272714075	25.667184956854616	25.658802848955148	23.76068392147616
22-23	24.51457634756415	25.355397686954912	25.651410021969184	24.478615943511755
24-25	24.21019518333187	25.576875645350754	25.86436242480488	24.348566746512493
26-27	24.014431473807676	25.701521266648424	25.79097065019187	24.493076609352027
28-29	24.128434479548172	25.985529946075932	25.64586286350461	24.240172710871292
30-31	24.24270547686787	25.82654470440958	25.477158414022437	24.45359140470011
32-33	24.022187132963822	25.88806489406102	25.433863745090463	24.6558842278847
34-35	24.38081931284147	25.86033304266492	25.538977735593754	24.219869908899856
36-37	24.42650479451754	25.632623163735634	25.538992573311038	24.401879468435787
38-39	24.225285928158108	25.807856528593653	25.504651649890235	24.462205893358004
40-41	24.380773677788458	25.72394715272016	25.65869391937698	24.236585250114402
42-43	24.271163438687278	25.794429526209857	25.480653602982734	24.45375343212014
44-45	24.15735057853043	25.785668030513264	25.611473451813936	24.44550793914237
46-47	24.309082829377353	25.874314506992675	25.433504565494214	24.38309809813576
48-49	24.126393865570243	25.923527380711526	25.350734115074065	24.599344638644165
50-51	24.031044775869827	26.06637904240392	25.426404258472225	24.476171923254025
52-53	24.384492944925373	25.767613648833805	25.308158511081917	24.539734895158904
54-55	24.080029029711977	25.873566908803898	25.388184606226005	24.658219455258124
56-57	24.05954032330215	25.982559359716916	25.31438889404509	24.64351142293584
58-59	24.091403585958393	25.580035092522824	25.435693840206753	24.89286748131203
60-61	24.017191403295012	25.689247889692744	25.574658043791214	24.71890266322103
62-63	24.008229093618745	25.619653675762844	25.66484366916828	24.707273561450137
64-65	23.92249649495499	25.702701957056618	25.46280388552109	24.9119976624673
66-67	23.930569025483244	25.777790783225203	25.442633435890578	24.849006755400975
68-69	24.236765757141246	25.40833538871865	25.496197885531064	24.85870096860904
70-71	24.042959632229632	25.44527802188718	25.51233974343901	24.999422602444174
72-73	24.016973903360316	25.51974140577795	25.502268823206936	24.9610158676548
74-75	23.861672987614035	25.697229703864892	25.592777576955193	24.848319731565873
76-77	23.948238532347887	25.400301252795245	25.741802608767266	24.909657606089606
78-79	23.956672974723045	25.5402083205312	25.633052543308843	24.870066161436917
80-81	24.042394965676205	25.558979156581884	25.561215574834733	24.837410302907177
82-83	23.958362618032798	25.50074876513063	25.720429734435807	24.820458882400764
84-85	23.841633380464764	25.540873128804044	25.65311901962725	24.964374471103948
86-87	23.872929136973674	25.42909595536455	25.731753617997978	24.966221289663793
88-89	23.941905118912	25.366834267481536	25.552538743210214	25.138721870396253
90-91	23.848959557489135	25.401672897244122	25.634474345387016	25.114893199879734
92-93	23.98297374645504	25.35917757995373	25.75829048589321	24.899558187698016
94-95	23.807266353570068	25.194891084543503	25.913734038035834	25.084108523850595
96-97	23.928827073090446	24.99454003019964	25.832062823614237	25.244570073095673
98-99	23.752434647880346	25.281830836396573	25.71196110540616	25.25377341031692
100-101	23.726833637728344	25.23699027419794	25.812185037893155	25.223991050180555
102-103	23.548541982431583	25.24579115079757	25.89491239041945	25.310754476351395
104-105	23.472945258762877	25.38098849017431	25.682728135160804	25.46333811590201
106-107	23.418015980671036	25.484833411014247	25.657316392623724	25.439834215690986
108-109	23.44793972163024	25.476339343704318	25.749950021271655	25.325770913393786
110-111	23.41001162260975	25.620052800781405	25.693837195047582	25.27609838156126
112-113	23.414118630929632	25.796228834997187	25.66264396664496	25.12700856742822
114-115	23.47447786063456	25.9414352756422	25.489729868251892	25.094356995471344
116-117	23.271777081416754	25.899195157441067	25.66152380988215	25.167503951260024
118-119	23.40124010948413	25.8464065983105	25.49414694659879	25.258206345606578
120-121	23.36344721719871	26.019424050807537	25.501183903923184	25.115944828070564
122-123	23.36562610027817	26.048055290829815	25.429825022524188	25.156493586367834
124-125	23.43193753504059	26.13045488695242	25.22100917734207	25.216598400664918
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2765.0
1	2099.0
2	1115.5
3	709.5
4	559.5
5	501.5
6	497.5
7	538.5
8	619.0
9	656.5
10	672.0
11	750.0
12	862.0
13	953.0
14	1093.5
15	1270.0
16	1414.0
17	1657.0
18	1999.5
19	2343.0
20	2798.0
21	3458.0
22	4471.5
23	5711.0
24	7063.0
25	9198.5
26	12243.5
27	15957.0
28	19830.0
29	24166.0
30	28944.0
31	35751.0
32	45227.0
33	50188.0
34	53121.0
35	59269.0
36	67154.5
37	75250.0
38	85226.0
39	95310.5
40	103508.0
41	113281.5
42	123059.0
43	135706.0
44	147630.0
45	157085.0
46	168283.5
47	177955.0
48	187042.5
49	195244.0
50	203627.5
51	210698.0
52	211325.5
53	213413.0
54	213004.0
55	204206.0
56	192599.5
57	178511.0
58	165110.0
59	149532.0
60	127654.5
61	106509.0
62	88085.0
63	68340.5
64	51537.0
65	40087.0
66	30877.5
67	23577.0
68	17930.5
69	13640.0
70	10697.5
71	8275.0
72	6379.5
73	5305.5
74	4275.0
75	3281.5
76	2439.5
77	1616.5
78	1117.0
79	784.0
80	518.0
81	359.5
82	284.5
83	215.5
84	149.0
85	115.0
86	95.5
87	71.0
88	41.0
89	37.5
90	33.0
91	25.0
92	15.5
93	14.5
94	17.0
95	13.0
96	14.5
97	13.0
98	10.5
99	12.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16836033121448585
2	0.14958167888671625
3	0.17519083100445318
4	0.14722129210914345
5	0.1727886674697552
6	0.11064574124604609
7	0.10924621988235247
8	0.09819626762274168
9	0.10419123227020538
10-11	0.09053023269027068
12-13	0.07895807096659509
14-15	0.07851941501678068
16-17	0.0779763171741533
18-19	0.07588747931789418
20-21	0.07000740075252472
22-23	0.061035842159891766
24-25	0.06540151327947334
26-27	0.07603369796783233
28-29	0.07106226386993558
30-31	0.06784545357129652
32-33	0.07681701216392949
34-35	0.0741432997079178
36-37	0.07640968878195896
38-39	0.0722111246908781
40-41	0.07686923311033597
42-43	0.07692145405674244
44-45	0.06962096574911679
46-47	0.06690547653597992
48-49	0.058800785653694496
50-51	0.06475397354403302
52-53	0.06696814167166769
54-55	0.06866010033523759
56-57	0.07242000847650402
58-59	0.05965720917476074
60-61	0.0696000773705542
62-63	0.06487930381540856
64-65	0.05888433916794487
66-67	0.06331267542321421
68-69	0.066237048421977
70-71	0.061672937706050805
72-73	0.05664928266174759
74-75	0.05979298363541758
76-77	0.06953741223486642
78-79	0.06251891703783574
80-81	0.060889623509953625
82-83	0.06559995287581796
84-85	0.06708302775376195
86-87	0.060628518777921234
88-89	0.05989742552823055
90-91	0.06300979393405665
92-93	0.05723415726150015
94-95	0.06693680910382381
96-97	0.05276404424910561
98-99	0.05267004654557395
100-101	0.05117652747834867
102-103	0.0489519151614327
104-105	0.04732262163355058
106-107	0.05892611592507004
108-109	0.05881122984297579
110-111	0.06562084125438056
112-113	0.0653075155759417
114-115	0.06808566992476632
116-117	0.07173069198393851
118-119	0.06467042002978265
120-121	0.06640415545047773
122-123	0.06369911042662216
124-125	0.07546971174664234
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4787351.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.16732314218836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98275847982403	35.15384111505971
2	12.50841970991484	12.04994188331172
3	5.198001333332359	7.5112142974843685
4	2.7572150487995732	5.312306729121348
5	1.668592689796194	4.018582164105327
6	1.0599012651989346	3.063156404379082
7	0.7508604499247823	2.531685654835115
8	0.5241543258695939	2.01976886324293
9	0.38343863038629705	1.6622291173513406
>10	2.001661101646565	16.89513291297005
>50	0.10892445743625083	3.564027599647331
>100	0.052329174575478504	4.3887173225753155
>500	0.0023504660756820906	0.7502632262127655
>1k	0.0013928672194829495	1.079132709703521
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.355351425036518E-5
2	0.0	0.0	0.0	0.0	8.355351425036518E-5
3	0.0	0.0	0.0	0.0	8.355351425036518E-5
4	0.0	0.0	0.0	0.0	8.355351425036518E-5
5	0.0	0.0	0.0	0.0	8.355351425036518E-5
6	0.0	0.0	0.0	0.0	1.0444189281295647E-4
7	0.0	0.0	0.0	0.0	1.0444189281295647E-4
8	0.0	0.0	0.0	0.0	1.0444189281295647E-4
9	0.0	0.0	0.0	0.0	1.0444189281295647E-4
10-11	0.0	0.0	0.0	1.0444189281295648E-5	1.0444189281295647E-4
12-13	0.0	0.0	0.0	2.0888378562591296E-5	3.759908141266433E-4
14-15	0.0	0.0	0.0	2.0888378562591296E-5	4.908768962208954E-4
16-17	0.0	0.0	0.0	4.177675712518259E-5	6.579839247216259E-4
18-19	0.0	0.0	0.0	6.266513568777388E-5	6.893164925655127E-4
20-21	0.0	0.0	0.0	1.1488608209425213E-4	7.833141960971736E-4
22-23	0.0	0.0	0.0	2.9243729987627815E-4	7.937583853784692E-4
24-25	0.0	0.0	0.0	6.370955461590345E-4	7.937583853784692E-4
26-27	0.0	0.0	0.0	0.0012010817673489996	8.668677103475387E-4
28-29	0.0	0.0	0.0	0.0034779150306714504	8.982002781914257E-4
30-31	0.0	0.0	0.0	0.013828106608435437	8.982002781914257E-4
32-33	0.0	0.0	0.0	0.04217363631787183	8.982002781914257E-4
34-35	0.0	0.0	0.0	0.08495303561405879	8.982002781914257E-4
36-37	0.0	0.0	0.0	0.15784303260822113	8.982002781914257E-4
38-39	0.0	0.0	0.0	0.2736899801163524	9.817537924417907E-4
40-41	0.0	0.0	0.0	0.4015999662443802	0.001002642171004382
42-43	0.0	0.0	0.0	0.5309303621146642	0.001033974738848269
44-45	0.0	2.0888378562591296E-5	0.0	0.6841361746819901	0.0010548631174108604
46-47	0.0	2.0888378562591296E-5	0.0	0.8699591903748023	0.0011279724423799299
48-49	0.0	2.0888378562591296E-5	0.0	1.0607223075976673	0.0012219701459115907
50-51	0.0	2.0888378562591296E-5	0.0	1.2639557868223994	0.0012950794708806604
52-53	0.0	2.0888378562591296E-5	0.0	1.4664895053652844	0.0014412981208187993
54-55	0.0	2.0888378562591296E-5	0.0	1.693838617640528	0.0014726306886626863
56-57	0.0	2.0888378562591296E-5	0.0	1.9633300336658	0.0015352958243504602
58-59	0.0	2.0888378562591296E-5	0.0	2.24852951037014	0.001577072581475643
60-61	0.0	2.0888378562591296E-5	0.0	2.549186387210798	0.0016397377171634168
62-63	0.0	2.0888378562591296E-5	0.0	2.855316019234855	0.0016710702850073035
64-65	0.0	2.0888378562591296E-5	0.0	3.181226945757685	0.001691958663569895
66-67	0.0	2.0888378562591296E-5	0.0	3.5574997529949233	0.0017024028528511907
68-69	0.0	2.0888378562591296E-5	0.0	3.9740349099115564	0.0017337354206950774
70-71	0.0	2.0888378562591296E-5	0.0	4.42273816981458	0.001838177313508034
72-73	0.0	2.0888378562591296E-5	0.0	4.877519947879318	0.0019948401527274686
74-75	0.0	2.0888378562591296E-5	0.0	5.344176769156888	0.0021306146133843124
76-77	0.0	2.0888378562591296E-5	0.0	5.858615756396388	0.0021723913705094947
78-79	0.0	2.0888378562591296E-5	0.0	6.423583731378794	0.0022037239383533814
80-81	0.0	2.0888378562591296E-5	0.0	7.020521369751247	0.00225594488475986
82-83	0.0	2.0888378562591296E-5	0.0	7.636519653562064	0.0023603867775728164
84-85	0.0	2.0888378562591296E-5	0.0	8.259912423384037	0.002370830966854112
86-87	0.0	2.0888378562591296E-5	0.0	8.913196462929083	0.0024439402918231814
88-89	0.0	2.0888378562591296E-5	0.0	9.620675400654767	0.0025066054275109553
90-91	0.0	2.0888378562591296E-5	0.0	10.374307210814498	0.0025066054275109553
92-93	0.0	2.0888378562591296E-5	0.0	11.148106750476412	0.002590158941761321
94-95	0.0	4.177675712518259E-5	0.0	11.910637009903807	0.002590158941761321
96-97	0.0	4.177675712518259E-5	0.0	12.701074143090825	0.0027781543488246425
98-99	0.0	6.26651356877739E-5	0.0	13.539554546971802	0.0028825962416375987
100-101	1.0444189281295648E-5	8.355351425036518E-5	0.0	14.440230098022894	0.0028825962416375987
102-103	3.133256784388695E-5	8.355351425036518E-5	0.0	15.365888149834847	0.002913928809481486
104-105	4.177675712518259E-5	8.355351425036518E-5	0.0	16.27091892781624	0.0029452613773253726
106-107	4.177675712518259E-5	8.355351425036518E-5	0.0	17.18569413439708	0.0029452613773253726
108-109	4.177675712518259E-5	8.355351425036518E-5	0.0	18.139561941457814	0.0029452613773253726
110-111	4.177675712518259E-5	8.355351425036518E-5	0.0	19.135331835915103	0.0029661497558879637
112-113	4.177675712518259E-5	1.0444189281295647E-4	0.0	20.163050505383872	0.0029661497558879637
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10835	0.0	37.401306	1
TCAACGC	12555	0.0	31.499825	4
GTACATG	18190	0.0	31.484549	1
GGTATCA	7655	0.0	30.90666	1
CAACGCA	12775	0.0	30.817493	5
ATCAACG	13000	0.0	30.332151	3
AACGCAG	12960	0.0	30.31006	6
TATCAAC	13155	0.0	30.291712	2
TACATGG	18890	0.0	29.798088	2
ACATGGG	18365	0.0	29.028236	3
CATGGGG	13135	0.0	28.113724	4
ACGCAGA	14505	0.0	26.87305	7
CGCAGAG	15155	0.0	25.561785	8
GTATAGG	1355	0.0	25.509119	1
GCAGAGT	17150	0.0	21.858932	9
ATGGGGA	7325	0.0	20.652987	5
ATGGGGG	6610	0.0	19.733286	5
AGAGTAC	14600	0.0	19.073368	10-11
TAAGGTG	1885	0.0	18.95818	5
CAGAGTA	15940	0.0	18.38452	10-11
>>END_MODULE