FastQCFastQC Report
Mon 15 Aug 2016
SRR2920909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4787351
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84070.17560859857570502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69070.14427603073181808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA139650.048.0217061
GGTATCA85400.044.363731
TCAACGC173850.037.9871944
TATCAAC177700.037.3991472
ATCAACG177800.037.1432723
CAACGCA178000.037.0680855
AACGCAG186350.035.4822246
ACGCAGA211150.031.0529757
CGCAGAG217500.030.0070828
GTACATG182550.027.8464571
ACATGGG181350.026.5650923
GCAGAGT241050.026.5344129
TACATGG189150.026.256972
CATGGGG130550.025.6353864
GAGTACT131150.023.70523812-13
AGAGTAC214950.023.2939410-11
AGTACTT139350.022.65190512-13
GTACTTT139500.022.20101514-15
CAGAGTA227000.022.04430610-11
TACTTTT150650.020.71584514-15
Produced by FastQC (version 0.11.5)