FastQCFastQC Report
Mon 15 Aug 2016
SRR2920908_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920908_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219692
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82200.030.3036981
AACGCAG89500.026.86726
TCAACGC91550.026.5446284
TATCAAC93400.026.338532
CAACGCA92250.026.2140735
GTACATG109450.026.1346451
ATCAACG93850.025.9583683
TACATGG109950.025.2452142
ACGCAGA99700.024.1158737
CGCAGAG101150.023.886368
ACATGGG111750.023.6659413
CATGGGG59500.022.9241564
GGTATCA61100.022.9201681
GTATAGG7250.022.1930471
GCAGAGT115850.019.5195859
GAGTACT38150.018.8704712-13
GTACTAA9250.017.3945481
GTATAGT10650.017.3461551
GTACCGT3455.9885315E-717.252226
AGAGTAC95750.017.05817610-11
Produced by FastQC (version 0.11.5)