FastQCFastQC Report
Mon 15 Aug 2016
SRR2920908_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920908_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219692
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64560.20051607420834044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53690.16675508092078373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37260.11572535509607751No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113100.034.9840051
GGTATCA77400.034.8510631
TCAACGC133150.029.5176494
ATCAACG134550.029.1662583
CAACGCA133800.029.1512665
AACGCAG137100.028.6511766
TATCAAC137850.028.4684892
ACGCAGA155600.024.971147
CGCAGAG159700.024.1768868
GAGTACT97000.022.5434512-13
GTACATG114950.022.324311
CATGGGG60150.022.2754174
AGTACTT107900.022.0859412-13
TACATGG116700.021.942362
ACATGGG113800.021.5592653
GGACCGT5050.021.2137346
GCAGAGT178900.021.0194459
GTACTTT106950.020.41832214-15
AGAGTAC157850.020.33675410-11
ACCGTCC4850.019.6263168
Produced by FastQC (version 0.11.5)