FastQCFastQC Report
Mon 15 Aug 2016
SRR2920907_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920907_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4592134
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102900.035.9062351
GGTATCA71200.032.97691
TCAACGC120200.029.9816044
AACGCAG120950.029.6767946
CAACGCA122950.029.2141155
ATCAACG124250.029.0052873
TATCAAC127000.028.846582
GTACATG173700.027.6521421
TACATGG172350.026.924592
ACATGGG173150.026.1806433
CATGGGG112200.025.6423764
ACGCAGA139450.025.5224257
CGCAGAG141800.024.9282998
GCAGAGT163050.020.5108559
AGAGTAC137750.020.107310-11
ATGGGGA69950.018.6492065
CAGAGTA150450.018.1726810-11
GAGTACT57350.017.11881812-13
GTATAGG11450.016.6547471
GTATTAG14000.016.6008421
Produced by FastQC (version 0.11.5)