##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920907_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4592134
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.527504859396526	31.0	30.0	34.0	25.0	34.0
2	29.661033628374085	31.0	30.0	34.0	25.0	34.0
3	29.813135243875724	31.0	30.0	34.0	25.0	34.0
4	33.21000563136877	37.0	35.0	37.0	26.0	37.0
5	33.29091180701609	37.0	35.0	37.0	27.0	37.0
6	33.258304526827835	37.0	35.0	37.0	26.0	37.0
7	33.16904297653335	37.0	35.0	37.0	26.0	37.0
8	33.067020256813066	37.0	35.0	37.0	26.0	37.0
9	34.476685131574996	39.0	35.0	39.0	25.0	39.0
10-11	34.48043817972211	39.0	35.0	39.0	26.0	39.0
12-13	34.410849400300606	39.0	35.0	39.0	25.0	39.0
14-15	35.549748983805785	40.0	36.0	41.0	25.0	41.0
16-17	35.53086495298264	40.0	36.0	41.0	25.0	41.0
18-19	35.52270306136537	40.0	36.0	41.0	25.0	41.0
20-21	35.5458616843498	40.0	36.0	41.0	25.0	41.0
22-23	35.46423296881145	40.0	36.0	41.0	25.0	41.0
24-25	35.45834583659797	40.0	35.5	41.0	25.0	41.0
26-27	35.386606531952246	40.0	35.0	41.0	25.0	41.0
28-29	35.276020364388316	40.0	35.0	41.0	25.0	41.0
30-31	35.12569776927241	40.0	35.0	41.0	24.0	41.0
32-33	34.98713724381736	40.0	35.0	41.0	24.0	41.0
34-35	34.91098332496395	39.5	35.0	41.0	24.0	41.0
36-37	34.76542267712571	39.0	34.0	41.0	23.0	41.0
38-39	34.621203562439604	39.0	34.0	41.0	22.0	41.0
40-41	34.482155681868164	39.0	34.0	41.0	20.5	41.0
42-43	34.29489720901002	39.0	33.5	41.0	19.0	41.0
44-45	34.13353149102356	39.0	33.0	41.0	18.0	41.0
46-47	33.917815660431515	38.5	33.0	41.0	16.0	41.0
48-49	33.780522846240984	38.0	33.0	40.5	15.0	41.0
50-51	33.56925538322706	38.0	33.0	40.0	13.0	41.0
52-53	33.36151198985047	38.0	33.0	40.0	10.5	41.0
54-55	33.109132268352795	38.0	32.0	40.0	8.0	41.0
56-57	32.832641316651475	37.5	32.0	40.0	7.5	41.0
58-59	32.55915376162804	37.0	31.5	40.0	6.5	41.0
60-61	32.281235586766414	37.0	31.0	40.0	2.0	41.0
62-63	31.96054318101345	36.0	31.0	39.5	2.0	41.0
64-65	31.662106441144793	36.0	31.0	39.0	2.0	41.0
66-67	31.311628319208452	35.0	30.5	39.0	2.0	40.5
68-69	30.97713339811077	35.0	30.0	38.5	2.0	40.0
70-71	30.638582410704913	35.0	30.0	37.5	2.0	40.0
72-73	30.69797037717105	35.0	30.5	37.0	2.0	39.5
74-75	30.689989120526533	35.0	31.0	37.0	2.0	39.0
76-77	30.429371399005344	35.0	31.0	36.5	2.0	39.0
78-79	30.143643565279238	35.0	31.0	36.0	2.0	38.5
80-81	29.88765658841837	35.0	30.0	36.0	2.0	37.0
82-83	29.655795540809567	35.0	30.0	35.0	2.0	37.0
84-85	29.432258292114298	35.0	30.0	35.0	2.0	36.5
86-87	29.249608134257407	35.0	30.0	35.0	2.0	36.0
88-89	29.0588836257827	34.5	30.0	35.0	2.0	36.0
90-91	28.89921298028324	34.0	30.0	35.0	2.0	36.0
92-93	28.751721313010464	34.0	29.0	35.0	2.0	35.0
94-95	28.644926737765054	34.0	29.0	35.0	2.0	35.0
96-97	28.553731119344512	34.0	29.0	35.0	2.0	35.0
98-99	28.431188745798792	34.0	29.0	35.0	2.0	35.0
100-101	28.31410951858112	34.0	29.0	35.0	2.0	35.0
102-103	26.97672084481855	32.5	26.0	34.5	2.0	35.0
104-105	27.980521474329798	34.0	29.0	35.0	2.0	35.0
106-107	28.13979894750458	34.0	29.0	35.0	2.0	35.0
108-109	28.082131096348668	34.0	29.0	35.0	2.0	35.0
110-111	27.98627609734385	34.0	29.0	35.0	2.0	35.0
112-113	27.875418487352505	34.0	29.0	35.0	2.0	35.0
114-115	27.77050745905934	34.0	29.0	35.0	2.0	35.0
116-117	27.564051266796657	34.0	27.5	35.0	2.0	35.0
118-119	27.37874003676722	34.0	27.0	35.0	2.0	35.0
120-121	27.208213980689585	34.0	27.0	35.0	2.0	35.0
122-123	26.926498878299284	34.0	27.0	35.0	2.0	35.0
124-125	25.627444669515306	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	192737.0
3	27790.0
4	18078.0
5	17493.0
6	22170.0
7	23069.0
8	22752.0
9	24470.0
10	26340.0
11	28208.0
12	28767.0
13	28519.0
14	28220.0
15	25453.0
16	21099.0
17	19413.0
18	18524.0
19	18643.0
20	19035.0
21	20306.0
22	24377.0
23	30548.0
24	41086.0
25	51262.0
26	58309.0
27	64990.0
28	76254.0
29	89548.0
30	106562.0
31	128936.0
32	161314.0
33	204945.0
34	276671.0
35	406395.0
36	732063.0
37	1128284.0
38	378960.0
39	544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.308022737936746	18.064723149979507	12.307380536722283	25.319873575361463
2	16.245307140997102	20.24444959148627	38.74115134091344	24.769091926603185
3	19.081130324492502	24.30225146041914	28.896556207539426	27.720062007548936
4	12.945100063617268	16.406014699798245	35.65554902008251	34.99333621650197
5	14.072962497826719	37.53609502100858	33.97797726523593	14.412965215928777
6	33.15273552056382	35.81855893235387	16.990909939158332	14.03779560792398
7	28.303535827314352	32.340276916348905	21.140710836694087	18.215476419642656
8	25.935542121139065	35.463190188061	19.94914937241066	18.652118318389277
9	26.044316884010378	16.261550142666213	19.267492006702884	38.42664096662053
10-11	25.169412651948274	26.342942647087668	27.433144939634822	21.054499761329232
12-13	25.5371839406994	23.934529518773243	27.315955670133874	23.212330870393483
14-15	22.989283187363384	25.343338031003753	24.55860586305446	27.1087729185784
16-17	23.15871371837949	27.91118443455619	25.604067562421033	23.326034284643285
18-19	23.29056541436493	27.09565563431174	26.901684274208666	22.71209467711467
20-21	24.15166672441533	26.36239442073844	26.497134032026754	22.988804822819482
22-23	23.792888254173146	26.199234694933693	26.40773140716378	23.600145643729377
24-25	23.513277613578186	26.329659879673486	26.56897713961779	23.588085367130546
26-27	23.259988362438136	26.435658884319587	26.590510374646676	23.713842378595604
28-29	23.36287845125557	26.665222571211956	26.516937648854245	23.45496132867823
30-31	23.527991478694123	26.51638669086383	26.33813310530821	23.617488725133843
32-33	23.29046035798938	26.620600211484497	26.231225956278674	23.857713474247447
34-35	23.643802109280372	26.630972259088352	26.315968403944147	23.409257227687128
36-37	23.69949194328289	26.360423335194845	26.310429464565427	23.629655256956834
38-39	23.45694240125507	26.57563179725566	26.282632901803748	23.684792899685526
40-41	23.623672413864497	26.426965082466005	26.48641810161367	23.462944402055836
42-43	23.452322992022268	26.54132419981859	26.29878422725226	23.70756858090688
44-45	23.455375405164048	26.51974925482939	26.410506960083087	23.614368379923476
46-47	23.511955705396915	26.606299867193332	26.23305963181751	23.648684795592242
48-49	23.33466466465812	26.6741164568458	26.122616268694475	23.868602609801606
50-51	23.34072768239455	26.76818279876838	26.205751031250202	23.68533848758686
52-53	23.609034435080865	26.561498453638293	26.069539153159266	23.759927958121573
54-55	23.42165978327251	26.557818059389497	26.080017346044322	23.94050481129367
56-57	23.318958702419426	26.72660375999549	26.034531584870884	23.919905952714192
58-59	23.32506314313639	26.3977258774589	26.18384052118649	24.093370458218217
60-61	23.275827690529482	26.367190011163938	26.38406775679397	23.97291454151261
62-63	23.275325944275252	26.35632521036676	26.451986085373953	23.91636275998403
64-65	23.205687883287705	26.381578875693034	26.255908990381105	24.156824250638152
66-67	23.207781087617317	26.596448580067218	26.08133460051728	24.114435731798185
68-69	23.45651248979227	26.2620184253922	26.218142217965713	24.06332686684982
70-71	23.265143854002083	26.220090293207786	26.259192364103146	24.25557348868698
72-73	23.303809130884627	26.167741381626346	26.260583506545306	24.26786598094372
74-75	23.180107308986607	26.416427199934457	26.24813590728476	24.155329583794185
76-77	23.291215235953416	26.089216710168543	26.38154278301961	24.23802527085843
78-79	23.282984385859383	26.2242451334986	26.35603265096758	24.13673782967444
80-81	23.31816574813165	26.211232365843678	26.295156160031553	24.175445725993118
82-83	23.25940945518888	26.071135366825615	26.474496180324213	24.19495899766129
84-85	23.202496130167997	26.218671575772056	26.336517997783186	24.242314296276756
86-87	23.18969297588574	26.126035629636124	26.355983677240364	24.328287717237767
88-89	23.271377271030165	26.02310412063396	26.27102569653314	24.43449291180274
90-91	23.15813706751637	26.038940727321975	26.36548962560077	24.437432579560888
92-93	23.226565202352127	25.954477239981806	26.503092682421965	24.315864875244106
94-95	23.16010630120425	25.8248056699496	26.5656912002533	24.44939682859285
96-97	23.20464703877213	25.656182011124752	26.581345456775757	24.55782549332736
98-99	23.070986696054266	25.917463724682122	26.382396984895063	24.629152594368552
100-101	23.0776630134139	25.80125108093157	26.535325900460393	24.585760005194135
102-103	22.93949982161775	25.818540315750198	26.556961129009853	24.684998733622194
104-105	22.822667730879385	26.003758877169165	26.432327084406104	24.741246307545346
106-107	22.75164451769512	26.062567226698043	26.35038165535459	24.835406600252256
108-109	22.787848758362706	26.098111658456485	26.40101459355248	24.71302498962833
110-111	22.77571002049308	26.205023827602318	26.33768570183699	24.68158045006761
112-113	22.802747898447347	26.335864472107534	26.33734624187233	24.52404138757279
114-115	22.775777757172865	26.480783694766192	26.19928326128211	24.54415528677884
116-117	22.611511085323897	26.422901224983452	26.332845737073935	24.63274195261872
118-119	22.76220281371325	26.425721705050176	26.118944897843114	24.693130583393458
120-121	22.748365303088182	26.551842560061644	26.096565823585006	24.60322631326517
122-123	22.804809834260187	26.561536797088632	26.09012370616892	24.54352966248226
124-125	22.838011063016303	26.587655291719386	25.917911739079834	24.65642190618447
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2820.0
1	2090.5
2	1111.0
3	771.0
4	611.0
5	532.5
6	496.0
7	515.5
8	587.0
9	633.0
10	631.0
11	663.5
12	768.5
13	906.0
14	1045.0
15	1201.0
16	1376.5
17	1581.0
18	1845.5
19	2282.5
20	2886.5
21	3617.5
22	4798.0
23	6239.5
24	7884.5
25	10297.5
26	14202.5
27	19252.0
28	24065.5
29	29920.5
30	37088.0
31	45731.0
32	55437.5
33	62176.5
34	68218.0
35	75839.5
36	84577.0
37	93428.5
38	102414.5
39	111072.5
40	119352.0
41	128997.5
42	137358.0
43	147217.0
44	157017.5
45	165463.5
46	173137.5
47	177408.0
48	182128.5
49	186711.0
50	191278.0
51	193895.0
52	190507.5
53	187224.0
54	182854.0
55	173497.0
56	160757.5
57	145752.5
58	132871.5
59	118451.5
60	99789.5
61	82971.0
62	68349.0
63	51652.0
64	37150.0
65	28126.0
66	21275.0
67	16256.5
68	12303.5
69	9453.5
70	7701.5
71	5907.0
72	4528.0
73	3856.5
74	3047.5
75	2241.0
76	1654.0
77	1142.5
78	772.0
79	532.5
80	357.5
81	234.0
82	166.0
83	114.0
84	78.0
85	62.5
86	54.5
87	38.5
88	29.5
89	25.5
90	22.0
91	17.5
92	14.0
93	15.5
94	18.0
95	17.0
96	11.5
97	9.5
98	8.0
99	11.5
100	22.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17205508375844433
2	0.1519119433361483
3	0.1780435849650729
4	0.1502787157343405
5	0.17536509169810813
6	0.11206118985203829
7	0.11142967517933929
8	0.09973576555039552
9	0.10568071402097587
10-11	0.09198337853381457
12-13	0.08089920720954571
14-15	0.08034390982493107
16-17	0.07918976231965356
18-19	0.07774163384605066
20-21	0.07149181622313286
22-23	0.062127977972768214
24-25	0.06614571787321537
26-27	0.07717544827742395
28-29	0.07258063462433804
30-31	0.06894398116431272
32-33	0.07878689951120764
34-35	0.07534623336339924
36-37	0.07777429839808682
38-39	0.07359323573745888
40-41	0.07920065050366562
42-43	0.07865624130306302
44-45	0.07081674881438564
46-47	0.06821447283550523
48-49	0.059732577490116796
50-51	0.06628726426537206
52-53	0.06830157830760165
54-55	0.06949927854892736
56-57	0.07357145936943478
58-59	0.060091887562514504
60-61	0.07079497244636154
62-63	0.0654597622804561
64-65	0.05942770833777934
66-67	0.06436005569523885
68-69	0.06721275990639645
70-71	0.062345741653009254
72-73	0.05710852514321229
74-75	0.0603205394267676
76-77	0.07080586063037358
78-79	0.06377209375858806
80-81	0.062095313420732066
82-83	0.06677723254591439
84-85	0.06773539273897496
86-87	0.06176866790037051
88-89	0.06052741492299659
90-91	0.06377209375858806
92-93	0.05817556717639338
94-95	0.0674740763226857
96-97	0.05353720078725926
98-99	0.05319966708288565
100-101	0.051022030280475264
102-103	0.049465019966751844
104-105	0.04789712146901637
106-107	0.059199056473526246
108-109	0.05901395734532137
110-111	0.06584084872087792
112-113	0.06591706600896229
114-115	0.06867177656401141
116-117	0.07178579719145826
118-119	0.06461048392751606
120-121	0.0665703570496854
122-123	0.0643056147751786
124-125	0.07647860450065264
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4592134.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.92312735690594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.60623700484605	30.820067224467717
2	13.843212575837406	12.43760803146131
3	6.200145702125998	8.355898050239375
4	3.4010550919820295	6.111441241798486
5	2.0422818815016166	4.58728445306993
6	1.353095397831548	3.647116612969796
7	0.929383386971473	2.9225565779418448
8	0.6353873015582727	2.2834867735090465
9	0.48322162349451053	1.9537043880469267
>10	2.342027080390143	17.94937702500058
>50	0.11184943073108136	3.4136898113644327
>100	0.048348508371265204	3.80266143941013
>500	0.002340788451324852	0.7182999116415262
>1k	0.0014142259073382849	0.9968084590788674
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.0888184012051913E-5	0.0
12-13	0.0	0.0	0.0	2.1776368024103826E-5	2.6131641628924596E-4
14-15	0.0	0.0	0.0	2.1776368024103826E-5	3.0486915233745356E-4
16-17	0.0	0.0	0.0	2.1776368024103826E-5	3.484218883856612E-4
18-19	0.0	0.0	0.0	3.266455203615574E-5	3.484218883856612E-4
20-21	0.0	0.0	0.0	4.355273604820765E-5	4.028628084459208E-4
22-23	0.0	0.0	0.0	1.5243457616872678E-4	4.137509924579727E-4
24-25	0.0	0.0	0.0	4.1375099245797274E-4	4.137509924579727E-4
26-27	0.0	0.0	0.0	8.166138009038934E-4	4.246391764700246E-4
28-29	0.0	0.0	0.0	0.0025369468748080957	4.3552736048207654E-4
30-31	0.0	0.0	0.0	0.010278445707377007	4.3552736048207654E-4
32-33	0.0	0.0	0.0	0.03143418724279387	4.3552736048207654E-4
34-35	0.0	0.0	0.0	0.06679900891393849	4.5730372850618034E-4
36-37	0.0	0.0	0.0	0.12891609870269466	4.5730372850618034E-4
38-39	0.0	0.0	0.0	0.23319223698611585	5.008564645543881E-4
40-41	0.0	0.0	0.0	0.35486769332079593	5.226328325784918E-4
42-43	0.0	0.0	0.0	0.4732440298998244	5.335210165905437E-4
44-45	0.0	0.0	0.0	0.6190477020052115	5.552973846146476E-4
46-47	0.0	0.0	0.0	0.7985394154438873	5.661855686266995E-4
48-49	0.0	0.0	0.0	0.9846293683938666	5.879619366508033E-4
50-51	0.0	0.0	0.0	1.1813679653076326	5.879619366508033E-4
52-53	0.0	0.0	0.0	1.3791627160705677	6.424028567110629E-4
54-55	0.0	0.0	0.0	1.6022616064775113	6.641792247351667E-4
56-57	0.0	0.0	0.0	1.8673671108029513	6.968437767713224E-4
58-59	0.0	0.0	0.0	2.151810029933795	7.186201447954263E-4
60-61	0.0	0.0	0.0	2.4515072948655243	7.186201447954263E-4
62-63	0.0	0.0	0.0	2.751150118877193	7.4039651281953E-4
64-65	0.0	0.0	0.0	3.0725148699929052	7.621728808436339E-4
66-67	0.0	0.0	0.0	3.4597313580135074	7.621728808436339E-4
68-69	0.0	0.0	0.0	3.8893246582090155	7.621728808436339E-4
70-71	0.0	0.0	0.0	4.349470202742342	7.730610648556858E-4
72-73	0.0	0.0	0.0	4.8143847718729464	8.275019849159455E-4
74-75	0.0	0.0	0.0	5.291494107097049	8.928310889882568E-4
76-77	0.0	0.0	0.0	5.8238174234462665	9.146074570123607E-4
78-79	0.0	0.0	0.0	6.408893991333876	9.254956410244126E-4
80-81	0.0	0.0	0.0	7.030173771061559	9.581601930605684E-4
82-83	0.0	0.0	0.0	7.667056318478512	0.0010670420331810875
84-85	0.0	0.0	0.0	8.306519365506322	0.0010670420331810875
86-87	0.0	0.0	0.0	8.984602801224877	0.0010997065852172434
88-89	0.0	0.0	0.0	9.721852628864925	0.001132371137253399
90-91	0.0	0.0	0.0	10.513608705669304	0.001132371137253399
92-93	0.0	0.0	0.0	11.31628563103777	0.001263029345398022
94-95	0.0	0.0	0.0	12.122446775290094	0.0012848057134221258
96-97	0.0	2.1776368024103826E-5	0.0	12.953258768145703	0.0013283584494703333
98-99	0.0	2.1776368024103826E-5	0.0	13.839916256799127	0.0013501348174944372
100-101	0.0	2.1776368024103826E-5	0.0	14.781951049337845	0.0013501348174944372
102-103	0.0	2.1776368024103826E-5	0.0	15.748832677792068	0.0013827993695305929
104-105	0.0	2.1776368024103826E-5	0.0	16.719285630602243	0.0014372402895908526
106-107	0.0	2.1776368024103826E-5	0.0	17.6877351575542	0.0014372402895908526
108-109	0.0	2.1776368024103826E-5	0.0	18.69890991856945	0.0014590166576149565
110-111	0.0	2.1776368024103826E-5	0.0	19.745689041304107	0.0014590166576149565
112-113	0.0	2.1776368024103826E-5	0.0	20.832351582074914	0.00148079302563906
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10290	0.0	35.906235	1
GGTATCA	7120	0.0	32.9769	1
TCAACGC	12020	0.0	29.981604	4
AACGCAG	12095	0.0	29.676794	6
CAACGCA	12295	0.0	29.214115	5
ATCAACG	12425	0.0	29.005287	3
TATCAAC	12700	0.0	28.84658	2
GTACATG	17370	0.0	27.652142	1
TACATGG	17235	0.0	26.92459	2
ACATGGG	17315	0.0	26.180643	3
CATGGGG	11220	0.0	25.642376	4
ACGCAGA	13945	0.0	25.522425	7
CGCAGAG	14180	0.0	24.928299	8
GCAGAGT	16305	0.0	20.510855	9
AGAGTAC	13775	0.0	20.1073	10-11
ATGGGGA	6995	0.0	18.649206	5
CAGAGTA	15045	0.0	18.17268	10-11
GAGTACT	5735	0.0	17.118818	12-13
GTATAGG	1145	0.0	16.654747	1
GTATTAG	1400	0.0	16.600842	1
>>END_MODULE