FastQCFastQC Report
Mon 15 Aug 2016
SRR2920907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4592134
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86140.18758163415963036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71780.15631076967701726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51330.11177809706772494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85100.048.0265771
GTATCAA134250.042.6655731
TCAACGC166950.033.9929164
ATCAACG171000.033.2922943
TATCAAC173750.032.8007132
CAACGCA172400.032.7456055
AACGCAG176650.032.0411076
ACGCAGA200200.027.998687
CGCAGAG204950.027.2582198
GTACATG169500.027.0693131
ACATGGG166100.026.3153043
CATGGGG110750.026.293424
TACATGG174900.025.6049652
GCAGAGT232350.023.4041449
AGAGTAC206250.022.63068410-11
GAGTACT130250.022.36033812-13
AGTACTT138100.022.16639712-13
CAGAGTA215150.021.52860810-11
GTACTTT140900.020.48030914-15
TACTTTT161350.018.65895514-15
Produced by FastQC (version 0.11.5)