##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920907_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4592134 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.982993309864217 31.0 31.0 34.0 28.0 34.0 2 31.133155739793306 33.0 31.0 34.0 27.0 34.0 3 31.209918525896676 33.0 31.0 34.0 27.0 34.0 4 34.736938643340984 37.0 35.0 37.0 32.0 37.0 5 34.42958393635726 37.0 35.0 37.0 30.0 37.0 6 34.14820778313525 37.0 35.0 37.0 30.0 37.0 7 34.04575106040024 37.0 35.0 37.0 30.0 37.0 8 34.04116778822221 37.0 35.0 37.0 30.0 37.0 9 35.56946574294217 39.0 35.0 39.0 30.0 39.0 10-11 35.53660509471196 39.0 35.0 39.0 30.0 39.0 12-13 35.43890552845366 39.0 35.0 39.0 29.5 39.0 14-15 36.64689063951531 40.0 37.0 41.0 30.0 41.0 16-17 36.657125750250316 40.0 37.0 41.0 30.0 41.0 18-19 36.69413969627193 40.0 37.0 41.0 30.0 41.0 20-21 36.68022470598637 40.0 37.0 41.0 30.0 41.0 22-23 36.63425588190589 40.0 37.0 41.0 29.5 41.0 24-25 36.61206957375373 40.0 37.0 41.0 30.0 41.0 26-27 36.488021582122826 40.0 36.0 41.0 29.0 41.0 28-29 36.386278362086124 40.0 36.0 41.0 29.0 41.0 30-31 36.23355710003236 40.0 36.0 41.0 28.0 41.0 32-33 36.07885135756056 40.0 36.0 41.0 28.0 41.0 34-35 35.95597275689255 40.0 35.0 41.0 27.0 41.0 36-37 35.82190654715215 40.0 35.0 41.0 27.0 41.0 38-39 35.6927243412322 40.0 35.0 41.0 26.5 41.0 40-41 35.552012855025566 40.0 35.0 41.0 26.0 41.0 42-43 35.40672898482492 40.0 35.0 41.0 25.0 41.0 44-45 35.20802528846066 39.5 35.0 41.0 24.5 41.0 46-47 34.99003393629192 39.0 35.0 41.0 24.0 41.0 48-49 34.73124183658403 39.0 34.5 41.0 23.0 41.0 50-51 34.537193165530454 39.0 34.0 41.0 22.0 41.0 52-53 34.327235006643974 39.0 34.0 41.0 21.5 41.0 54-55 34.11607751428856 38.5 34.0 41.0 20.0 41.0 56-57 33.86049971538287 38.0 33.5 40.5 19.0 41.0 58-59 33.62536252208668 38.0 33.0 40.0 18.0 41.0 60-61 33.3574290297278 37.0 33.0 40.0 17.5 41.0 62-63 33.06313132412948 37.0 33.0 40.0 14.0 41.0 64-65 32.748148682072426 36.0 32.5 39.5 13.0 41.0 66-67 32.42503550636806 36.0 32.0 39.0 9.5 41.0 68-69 32.061680038082514 35.0 32.0 39.0 8.0 41.0 70-71 31.726208446878946 35.0 31.5 38.5 7.0 40.0 72-73 31.371032291305088 35.0 31.0 37.0 6.5 40.0 74-75 31.044789198224613 35.0 31.0 37.0 2.0 39.0 76-77 30.835767423163176 35.0 31.0 36.0 2.0 39.0 78-79 30.686685318851758 35.0 31.0 36.0 2.0 38.5 80-81 30.443031279139504 35.0 31.0 36.0 2.0 37.0 82-83 30.225459448700757 35.0 31.0 35.0 2.0 37.0 84-85 29.98716195999507 35.0 31.0 35.0 2.0 36.5 86-87 29.82394786389073 35.0 31.0 35.0 2.0 36.0 88-89 29.673662724127823 35.0 31.0 35.0 2.0 36.0 90-91 29.521107397998403 35.0 30.5 35.0 2.0 35.5 92-93 29.403682906465708 35.0 30.0 35.0 2.0 35.0 94-95 29.311036001998197 34.5 30.0 35.0 2.0 35.0 96-97 29.241017683717416 34.0 30.0 35.0 2.0 35.0 98-99 29.13527196723789 34.0 30.0 35.0 2.0 35.0 100-101 29.035789896374975 34.0 30.0 35.0 2.0 35.0 102-103 27.633630573498074 32.5 27.0 34.5 2.0 35.0 104-105 28.693062092700256 34.0 29.0 35.0 2.0 35.0 106-107 28.872233693528976 34.0 30.0 35.0 2.0 35.0 108-109 28.97215466708942 34.5 30.0 35.0 2.0 35.0 110-111 29.143452477649824 35.0 31.0 35.0 2.0 35.0 112-113 29.11728479613182 35.0 31.0 35.0 2.0 35.0 114-115 29.034110176227436 35.0 31.0 35.0 2.0 35.0 116-117 28.92349809478556 35.0 31.0 35.0 2.0 35.0 118-119 28.818027958243377 35.0 30.5 35.0 2.0 35.0 120-121 28.63504581965596 35.0 30.0 35.0 2.0 35.0 122-123 28.44450782577338 35.0 30.0 35.0 2.0 35.0 124-125 27.714758214808192 34.5 28.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100238.0 3 36886.0 4 15363.0 5 13112.0 6 15055.0 7 17797.0 8 19469.0 9 21645.0 10 24327.0 11 27015.0 12 28285.0 13 31881.0 14 38511.0 15 34892.0 16 22602.0 17 19539.0 18 18538.0 19 17919.0 20 17839.0 21 17855.0 22 18906.0 23 21996.0 24 29431.0 25 39364.0 26 48589.0 27 57676.0 28 69126.0 29 83368.0 30 99919.0 31 121824.0 32 149931.0 33 192685.0 34 258614.0 35 384617.0 36 723946.0 37 1266843.0 38 485523.0 39 1008.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95819570794283 18.55335391303494 12.988803376555072 25.499647002467164 2 16.365985639875753 20.55851122329799 37.96303495208682 25.112468184739434 3 18.99745019229718 24.254095774252843 29.62876986205255 27.119684171397424 4 13.331761040187335 16.693724481540688 35.393472892860416 34.58104158541156 5 13.890263753972306 37.28202576580711 34.26705016139863 14.560660318821949 6 33.17168550597034 36.03611376747508 16.61536597795846 14.17683474859612 7 28.170588236575426 32.1192770640819 21.233154958373003 18.476979740969675 8 26.333561656515542 34.9837360569494 19.77791305351583 18.904789233019233 9 26.32881111119823 15.223994173642527 19.402889831490384 39.044304883668865 10-11 25.468579121467027 25.760698211012865 27.554552907845935 21.216169759674173 12-13 26.005695479062403 23.261330929199843 27.65395636079556 23.079017230942192 14-15 23.22712917710374 24.607551242816562 25.159541466732747 27.005778113346945 16-17 23.03361786476511 27.38728746859015 26.137253571599324 23.441841095045422 18-19 23.17165208826628 26.22696303085521 27.98493101841738 22.616453862461128 20-21 23.890824937467283 25.502731031833125 27.741755694590957 22.86468833610863 22-23 23.518149902477372 25.406454995030494 27.71690984980176 23.358485252690382 24-25 23.30516175688624 25.652312778220683 27.78641015000001 23.256115314893076 26-27 23.056072349310835 25.73823466157042 27.872332432233776 23.333360556884973 28-29 23.134835215777315 25.917855465458 27.789649340428614 23.157659978336078 30-31 23.323690845843796 25.74035628746264 27.67464690038401 23.26130596630955 32-33 23.08118850696127 25.857398766191693 27.538906342733 23.522506384114035 34-35 23.38590475941562 25.85799779913305 27.619845699733574 23.136251741717754 36-37 23.501747647079835 25.59467699456975 27.52559396913652 23.377981389213897 38-39 23.329163395459855 25.751081608265213 27.481524147686585 23.438230848588347 40-41 23.412108552041555 25.650548714062193 27.640238210807393 23.297104523088855 42-43 23.384890860577304 25.676474057136733 27.444067359518925 23.494567722767037 44-45 23.35077576152262 25.711427821562328 27.42567184204021 23.512124574874846 46-47 23.49203220442163 25.788944077869097 27.157239950819978 23.5617837668893 48-49 23.415947751309282 25.969859188458642 26.8355523795346 23.77864068069747 50-51 23.365676931438564 26.156486998039085 26.86708568184053 23.61075038868182 52-53 23.629174980801807 25.984421913337858 26.748451186062834 23.637951919797505 54-55 23.33638653612709 26.040099068825782 26.702627014016674 23.920887381030454 56-57 23.284253748434647 26.206420455232287 26.573632500231692 23.93569329610137 58-59 23.173716626003458 25.934241035472173 26.780665221347682 24.111377117176687 60-61 23.17094061306333 26.01801782179835 26.905219941029614 23.905821624108707 62-63 23.086658611107634 26.009171139607485 26.993797146938082 23.910373102346803 64-65 22.962150323205805 26.005777825113196 26.845280916471786 24.18679093520921 66-67 22.9410343829425 26.224260275252597 26.696413422108943 24.13829191969596 68-69 23.197235996899895 25.92872218216594 26.74682859349751 24.127213227436652 70-71 22.99627598828641 25.882409568544105 26.780606738286494 24.340707704882988 72-73 23.070717934928744 25.895048589059865 26.64456305785175 24.389670418159636 74-75 22.981981693552235 26.135953045379317 26.496859303185865 24.38520595788258 76-77 23.05081663466046 25.868781998265295 26.646252388214677 24.43414897885957 78-79 23.015729199631398 26.038512897055078 26.52548886537148 24.420269037942045 80-81 22.985252019847216 26.115001074297034 26.399099006190845 24.500647899664905 82-83 22.8700634664886 26.026182403920934 26.553487193995412 24.55026693559505 84-85 22.790084549006625 26.10849149064995 26.390103715190506 24.711320245152915 86-87 22.778485684997975 26.037085287580155 26.366800289977576 24.8176287374443 88-89 22.75710645022811 26.017525973258017 26.273441599074086 24.951925977439785 90-91 22.564607000352165 26.086289893498275 26.350535078531976 24.998568027617573 92-93 22.64299965567311 26.062170060896424 26.374361955297616 24.92046832813286 94-95 22.537883531485413 25.892152620043916 26.472876987882195 25.097086860588476 96-97 22.621849134820888 25.750297779995414 26.402702224142686 25.225150861041012 98-99 22.46978689113698 25.939954917184828 26.263244438152693 25.327013753525495 100-101 22.421296167733075 25.852623715176986 26.328399591187107 25.397680525902828 102-103 22.21711810346339 25.84043980223754 26.36669041933312 25.57575167496594 104-105 22.197816317619402 26.00922081499896 26.174134895253154 25.61882797212849 106-107 22.0861809018414 26.12278280572057 26.047191254464767 25.743845037973266 108-109 22.05790574866026 26.150607043025463 26.079660396972347 25.711826811341925 110-111 21.976134165093786 26.241561677544983 26.049549108578407 25.732755048782824 112-113 21.812986895327484 26.47482283875697 26.00512966706819 25.70706059884736 114-115 21.8636568693414 26.63203065170813 25.804302994752547 25.70000948419792 116-117 21.68339669795481 26.57279861113228 25.93223085134786 25.811573839565042 118-119 21.7132267963177 26.609602442847034 25.680218222093593 25.996952538741674 120-121 21.611682245839965 26.807099172740234 25.671282927717336 25.90993565370246 122-123 21.48375980822567 26.893625611489735 25.603334361193575 26.019280219091023 124-125 21.417306768063753 27.013088316027208 25.49204494403554 26.077559971873505 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 117.0 1 136.5 2 325.0 3 528.5 4 396.5 5 244.0 6 409.5 7 998.5 8 1641.0 9 1774.0 10 1473.5 11 1199.5 12 1278.0 13 1501.0 14 1664.0 15 1867.0 16 2135.5 17 2378.5 18 2725.0 19 3104.0 20 3575.0 21 4324.5 22 5540.0 23 6956.0 24 8410.0 25 10601.0 26 14380.0 27 19306.0 28 24358.0 29 30605.5 30 37723.5 31 46171.0 32 56141.5 33 62751.5 34 68278.0 35 75781.0 36 83950.0 37 92627.5 38 101574.5 39 110335.5 40 118800.5 41 127848.0 42 136577.5 43 146486.5 44 156273.0 45 165179.0 46 173359.5 47 178138.5 48 183134.5 49 188143.5 50 193546.5 51 196162.0 52 191662.5 53 188315.0 54 184146.0 55 173479.5 56 159680.5 57 144904.5 58 132046.0 59 118040.0 60 99430.5 61 82000.0 62 67235.5 63 50541.0 64 36106.5 65 27143.0 66 20104.5 67 15237.0 68 11594.0 69 8831.5 70 7125.0 71 5506.5 72 4201.5 73 3440.0 74 2680.5 75 2027.5 76 1415.5 77 861.0 78 519.5 79 321.5 80 202.5 81 126.5 82 78.5 83 55.0 84 35.5 85 25.5 86 17.5 87 15.5 88 13.0 89 8.0 90 8.0 91 7.5 92 8.0 93 8.5 94 7.0 95 14.0 96 16.0 97 8.5 98 4.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21665308547180895 2 0.008514559897424596 3 5.008564645543881E-4 4 0.00250428232277194 5 0.08285908033171506 6 0.05502888199691037 7 0.027068025453961055 8 0.015003917568607536 9 0.012760951662124842 10-11 0.011182164980377315 12-13 0.013011379894402035 14-15 0.020241134078404506 16-17 0.022179230832549746 18-19 0.021362617031645853 20-21 0.016060071417776572 22-23 0.013838881879317982 24-25 0.01235808885367892 26-27 0.011356375924570145 28-29 0.013708223671173358 30-31 0.010376439363485474 32-33 0.014350626527884422 34-35 0.016582704250355063 36-37 0.01781306904371693 38-39 0.02043712139062144 40-41 0.02156949252787484 42-43 0.020023370398163466 44-45 0.01827037277222311 46-47 0.01827037277222311 48-49 0.017083560714909452 50-51 0.014230856503751851 52-53 0.012107660621401728 54-55 0.016996455242813034 56-57 0.01997981766211526 58-59 0.01655003969831891 60-61 0.018433695532403892 62-63 0.023986669378550363 64-65 0.017138001634969712 66-67 0.020317351366488868 68-69 0.014753489336330342 70-71 0.015559214953222184 72-73 0.022396994512790785 74-75 0.013871546431354137 76-77 0.012554076165895855 78-79 0.01604918323376452 80-81 0.016539151514306856 82-83 0.012564964349907908 84-85 0.015548326769210133 86-87 0.016212505993945296 88-89 0.016920237954728672 90-91 0.01230364793361866 92-93 0.01234720066966687 94-95 0.013653782751113099 96-97 0.014307073791836213 98-99 0.012717398926076633 100-101 0.01723599529107818 102-103 0.023311601969803145 104-105 0.007599952440412235 106-107 0.007643505176460443 108-109 0.014274409239800058 110-111 0.011051506772232692 112-113 0.008993639993954881 114-115 0.006217153070881642 116-117 0.0041810626606279344 118-119 0.00250428232277194 120-121 0.0023082950105550054 122-123 0.0015243457616872678 124-125 5.008564645543881E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4592134.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.08031342427539 #Duplication Level Percentage of deduplicated Percentage of total 1 67.19852832468352 28.949336618774137 2 14.174333215519239 12.21269435009372 3 6.540598585481818 8.453131111347869 4 3.5289422224110543 6.081117479905086 5 2.1870355397860735 4.710908826200668 6 1.4569105061596566 3.765849674188662 7 0.9921366875241971 2.991909162078535 8 0.7159274482252986 2.467390308687005 9 0.5184857779126647 2.0102876836656147 >10 2.508596051017387 18.39252195709362 >50 0.11932806905020135 3.51079338892228 >100 0.054368601532390655 4.108873137405148 >500 0.0032903487901597005 0.9916122869025101 >1k 0.0013667597157372992 0.8972506285306455 >5k 1.5186219063743244E-4 0.45632338620454593 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8614 0.18758163415963036 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7178 0.15631076967701726 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5133 0.11177809706772494 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.71054720964153E-5 2 0.0 0.0 0.0 0.0 8.71054720964153E-5 3 0.0 0.0 0.0 0.0 8.71054720964153E-5 4 0.0 0.0 0.0 0.0 8.71054720964153E-5 5 0.0 0.0 0.0 0.0 8.71054720964153E-5 6 0.0 0.0 0.0 0.0 1.0888184012051914E-4 7 0.0 0.0 0.0 0.0 1.0888184012051914E-4 8 0.0 0.0 0.0 0.0 1.0888184012051914E-4 9 0.0 0.0 0.0 0.0 1.0888184012051914E-4 10-11 0.0 0.0 0.0 0.0 1.0888184012051914E-4 12-13 0.0 0.0 0.0 0.0 3.919746244338689E-4 14-15 0.0 0.0 0.0 0.0 4.4641554449412847E-4 16-17 0.0 0.0 0.0 0.0 5.335210165905437E-4 18-19 0.0 0.0 0.0 1.0888184012051913E-5 5.444092006025957E-4 20-21 0.0 0.0 0.0 2.1776368024103826E-5 6.532910407231148E-4 22-23 0.0 0.0 0.0 1.3065820814462295E-4 6.532910407231148E-4 24-25 0.0 0.0 0.0 3.70198256409765E-4 6.532910407231148E-4 26-27 0.0 0.0 0.0 7.730610648556858E-4 6.750674087472187E-4 28-29 0.0 0.0 0.0 0.0024389532186996285 6.968437767713224E-4 30-31 0.0 0.0 0.0 0.0102348929713288 7.077319607833744E-4 32-33 2.1776368024103826E-5 0.0 0.0 0.031347081770697456 7.186201447954263E-4 34-35 2.1776368024103826E-5 0.0 0.0 0.06650502794561308 7.186201447954263E-4 36-37 2.1776368024103826E-5 0.0 0.0 0.12832813676604385 7.295083288074782E-4 38-39 2.1776368024103826E-5 0.0 0.0 0.23156989756832008 7.839492488677378E-4 40-41 2.1776368024103826E-5 0.0 0.0 0.352788050174494 7.839492488677378E-4 42-43 2.1776368024103826E-5 0.0 0.0 0.47044576660872706 7.948374328797897E-4 44-45 2.1776368024103826E-5 0.0 0.0 0.6153021667050657 8.057256168918416E-4 46-47 3.266455203615574E-5 0.0 0.0 0.7934981862463073 8.057256168918416E-4 48-49 4.355273604820765E-5 0.0 0.0 0.978629979003226 8.275019849159454E-4 50-51 4.355273604820765E-5 0.0 0.0 1.1745628503001 8.492783529400492E-4 52-53 4.355273604820765E-5 0.0 0.0 1.3717151982063243 8.710547209641531E-4 54-55 4.355273604820765E-5 0.0 0.0 1.5943023439647015 8.710547209641531E-4 56-57 4.355273604820765E-5 0.0 0.0 1.8596582765224186 8.710547209641531E-4 58-59 4.355273604820765E-5 0.0 0.0 2.144416952989612 8.710547209641531E-4 60-61 4.355273604820765E-5 0.0 0.0 2.4447457325940403 8.710547209641531E-4 62-63 4.355273604820765E-5 0.0 0.0 2.744279674765588 9.146074570123607E-4 64-65 4.355273604820765E-5 0.0 0.0 3.0661343941618426 9.363838250364645E-4 66-67 4.355273604820765E-5 0.0 0.0 3.452512492013517 9.363838250364645E-4 68-69 4.355273604820765E-5 0.0 0.0 3.8796668389903255 9.363838250364645E-4 70-71 4.355273604820765E-5 0.0 0.0 4.335968854567398 9.363838250364645E-4 72-73 4.355273604820765E-5 0.0 0.0 4.796266833676892 9.472720090485165E-4 74-75 4.355273604820765E-5 0.0 0.0 5.270338365561631 9.799365610846723E-4 76-77 4.355273604820765E-5 0.0 0.0 5.799602102203464 0.0010017129291087761 78-79 4.355273604820765E-5 0.0 0.0 6.382566362392735 0.001012601113120828 80-81 4.355273604820765E-5 0.0 0.0 6.999469092147573 0.0010234892971328798 82-83 4.355273604820765E-5 0.0 0.0 7.633074296176897 0.0010452656651569836 84-85 4.355273604820765E-5 0.0 0.0 8.270490364610442 0.0010561538491690356 86-87 4.355273604820765E-5 0.0 0.0 8.945742872485862 0.0010888184012051913 88-89 4.355273604820765E-5 0.0 0.0 9.680172660466791 0.0011105947692292952 90-91 4.355273604820765E-5 0.0 0.0 10.468771163907673 0.0011105947692292952 92-93 4.355273604820765E-5 0.0 0.0 11.269150682449599 0.001154147505277503 94-95 4.355273604820765E-5 0.0 0.0 12.073591493628017 0.0011650356892895547 96-97 4.355273604820765E-5 0.0 0.0 12.902596047937624 0.0012956938974341776 98-99 4.355273604820765E-5 0.0 0.0 13.787544091701157 0.0013501348174944372 100-101 4.355273604820765E-5 0.0 0.0 14.729470002399756 0.0013501348174944372 102-103 4.355273604820765E-5 0.0 0.0 15.695284588820797 0.0013610230015064892 104-105 4.355273604820765E-5 0.0 0.0 16.665911752575163 0.001371911185518541 106-107 6.532910407231148E-5 0.0 0.0 17.639086751388355 0.001371911185518541 108-109 6.532910407231148E-5 0.0 0.0 18.65741504929952 0.001371911185518541 110-111 6.532910407231148E-5 0.0 0.0 19.713982649461013 0.001371911185518541 112-113 6.532910407231148E-5 0.0 0.0 20.80998725211416 0.001371911185518541 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8510 0.0 48.026577 1 GTATCAA 13425 0.0 42.665573 1 TCAACGC 16695 0.0 33.992916 4 ATCAACG 17100 0.0 33.292294 3 TATCAAC 17375 0.0 32.800713 2 CAACGCA 17240 0.0 32.745605 5 AACGCAG 17665 0.0 32.041107 6 ACGCAGA 20020 0.0 27.99868 7 CGCAGAG 20495 0.0 27.258219 8 GTACATG 16950 0.0 27.069313 1 ACATGGG 16610 0.0 26.315304 3 CATGGGG 11075 0.0 26.29342 4 TACATGG 17490 0.0 25.604965 2 GCAGAGT 23235 0.0 23.404144 9 AGAGTAC 20625 0.0 22.630684 10-11 GAGTACT 13025 0.0 22.360338 12-13 AGTACTT 13810 0.0 22.166397 12-13 CAGAGTA 21515 0.0 21.528608 10-11 GTACTTT 14090 0.0 20.480309 14-15 TACTTTT 16135 0.0 18.658955 14-15 >>END_MODULE