FastQCFastQC Report
Mon 15 Aug 2016
SRR2920906_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920906_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5398080
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91650.040.245611
GTATCAA133300.039.6509671
GTACATG259250.034.6841281
TACATGG266300.033.126762
CATGGGG197650.032.727434
ACATGGG266800.032.416923
TCAACGC161100.032.31434
TATCAAC164450.031.7658672
CAACGCA164650.031.4728765
AACGCAG167950.030.8707436
ATCAACG169200.030.76823
ATGGGGG117700.027.9851465
ACGCAGA189700.027.0455937
CGCAGAG199100.025.615678
GCAGAGT217500.022.2994679
TACCGTA4400.021.6433187
AGAGTAC192000.020.5940410-11
ATGGGGA78100.020.5154085
GTACCGT8800.020.2930566
CAGAGTA204450.020.09668410-11
Produced by FastQC (version 0.11.5)