##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920906_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5398080
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.766630357460432	31.0	30.0	34.0	25.0	34.0
2	29.868975635781613	31.0	30.0	34.0	25.0	34.0
3	30.00784464105756	31.0	31.0	34.0	25.0	34.0
4	33.416318579939535	37.0	35.0	37.0	27.0	37.0
5	33.49557101784338	37.0	35.0	37.0	28.0	37.0
6	33.473790310628964	37.0	35.0	37.0	27.0	37.0
7	33.39656748325331	37.0	35.0	37.0	27.0	37.0
8	33.30187659315905	37.0	35.0	37.0	27.0	37.0
9	34.72970685873496	39.0	35.0	39.0	27.0	39.0
10-11	34.723445651046305	39.0	35.0	39.0	27.0	39.0
12-13	34.64958299988144	39.0	35.0	39.0	27.0	39.0
14-15	35.7961929056257	40.0	36.0	41.0	27.0	41.0
16-17	35.77718401728022	40.0	36.0	41.0	27.0	41.0
18-19	35.7668530292252	40.0	36.0	41.0	27.0	41.0
20-21	35.79425860676389	40.0	36.0	41.0	26.5	41.0
22-23	35.7205352643903	40.0	36.0	41.0	26.0	41.0
24-25	35.71750752119272	40.0	36.0	41.0	27.0	41.0
26-27	35.643188689311756	40.0	35.5	41.0	26.5	41.0
28-29	35.532124477592035	40.0	35.0	41.0	26.0	41.0
30-31	35.379638223220105	40.0	35.0	41.0	25.0	41.0
32-33	35.24067575878831	40.0	35.0	41.0	25.0	41.0
34-35	35.169762582251465	40.0	35.0	41.0	25.0	41.0
36-37	35.035552918815576	39.0	35.0	41.0	24.0	41.0
38-39	34.89688972745865	39.0	35.0	41.0	24.0	41.0
40-41	34.755416555516035	39.0	34.0	41.0	23.5	41.0
42-43	34.56993440260241	39.0	34.0	41.0	22.5	41.0
44-45	34.4190241715573	39.0	34.0	41.0	22.0	41.0
46-47	34.202612780840596	39.0	33.0	41.0	20.5	41.0
48-49	34.062655333007285	38.5	33.0	41.0	19.5	41.0
50-51	33.8541657404114	38.0	33.0	40.0	18.0	41.0
52-53	33.6414990144644	38.0	33.0	40.0	17.0	41.0
54-55	33.387716373229	38.0	33.0	40.0	14.5	41.0
56-57	33.10591349146363	37.5	32.0	40.0	13.0	41.0
58-59	32.82264323611358	37.0	32.0	40.0	9.5	41.0
60-61	32.52776950323078	36.5	31.5	40.0	8.0	41.0
62-63	32.18459628238188	36.0	31.0	39.5	7.5	41.0
64-65	31.882523786235105	35.5	31.0	39.0	7.0	41.0
66-67	31.519393376904382	35.0	31.0	39.0	4.0	40.5
68-69	31.175716365818957	35.0	30.5	38.0	2.0	40.0
70-71	30.8332324641354	35.0	30.0	37.0	2.0	40.0
72-73	30.886181290384727	35.0	31.0	37.0	2.0	39.0
74-75	30.867783174758433	35.0	31.0	37.0	2.0	39.0
76-77	30.61393328739107	35.0	31.0	36.0	2.0	39.0
78-79	30.33987825300848	35.0	31.0	36.0	2.0	37.5
80-81	30.087559002460132	35.0	31.0	35.5	2.0	37.0
82-83	29.866081458592685	35.0	31.0	35.0	2.0	37.0
84-85	29.656660886833837	35.0	30.0	35.0	2.0	36.0
86-87	29.47273475013338	35.0	30.0	35.0	2.0	36.0
88-89	29.294538428478276	35.0	30.0	35.0	2.0	36.0
90-91	29.13590128341929	34.5	30.0	35.0	2.0	35.0
92-93	28.977085834222535	34.0	30.0	35.0	2.0	35.0
94-95	28.861047724376075	34.0	30.0	35.0	2.0	35.0
96-97	28.760559680478984	34.0	29.5	35.0	2.0	35.0
98-99	28.625591413984232	34.0	29.0	35.0	2.0	35.0
100-101	28.49729366737803	34.0	29.0	35.0	2.0	35.0
102-103	27.14175456829095	32.5	26.0	34.5	2.0	35.0
104-105	28.132779895814807	34.0	29.0	35.0	2.0	35.0
106-107	28.25898208251823	34.0	29.0	35.0	2.0	35.0
108-109	28.17326391976406	34.0	29.0	35.0	2.0	35.0
110-111	28.052373992234276	34.0	29.0	35.0	2.0	35.0
112-113	27.91809300714328	34.0	29.0	35.0	2.0	35.0
114-115	27.784545523593575	34.0	29.0	35.0	2.0	35.0
116-117	27.546713553707985	34.0	27.5	35.0	2.0	35.0
118-119	27.31877926966625	34.0	27.0	35.0	2.0	35.0
120-121	27.10961638212105	34.0	27.0	35.0	2.0	35.0
122-123	26.795745802211158	34.0	25.5	35.0	2.0	35.0
124-125	25.486608479311162	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	201129.0
3	30449.0
4	20137.0
5	19032.0
6	23600.0
7	25464.0
8	25565.0
9	26972.0
10	29576.0
11	31446.0
12	31811.0
13	31667.0
14	32653.0
15	29241.0
16	23815.0
17	22243.0
18	21394.0
19	21309.0
20	22024.0
21	23886.0
22	28887.0
23	37261.0
24	50378.0
25	64295.0
26	72392.0
27	78714.0
28	91320.0
29	107103.0
30	128371.0
31	155918.0
32	195473.0
33	250741.0
34	342490.0
35	497515.0
36	885107.0
37	1330575.0
38	387818.0
39	309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.791369968832	17.823060683988903	12.175022512697197	25.2105468344819
2	16.392557295633132	20.175928923453387	38.50708084697449	24.924432933938995
3	19.005284792198125	24.618142727430797	29.176955641259124	27.19961683911195
4	13.40092866592348	16.249742825694906	35.071321461295476	35.278007047086135
5	14.140926802130718	37.89684211229505	33.422731788675485	14.539499296898747
6	33.0660072065857	35.172737371320906	17.27071429763607	14.490541124457327
7	28.527495580274326	32.5699612948308	20.9323177870383	17.970225337856572
8	26.72338151480898	35.13463147401493	19.687521324159864	18.45446568701622
9	27.150877107095	15.764469698739795	18.931115781696967	38.15353741246824
10-11	25.76225213119842	26.022685069902124	26.97059718426122	21.244465614638237
12-13	25.806869342594023	23.591103082408253	27.135822978967017	23.466204596030703
14-15	23.64645213534839	24.769155158026773	24.374447417505888	27.20994528911895
16-17	23.712561684210215	27.422985810546002	25.247858456219735	23.616594049024044
18-19	23.89804992667493	26.860659007044784	26.417594086757816	22.823696979522463
20-21	24.723841977094665	25.907722618212652	26.1282653067183	23.24017009797438
22-23	24.616902012311147	25.51815693678426	25.931726021394596	23.93321502951
24-25	24.129134365913206	25.717923163311678	26.027191070157972	24.125751400617148
26-27	23.768295498775007	25.863947607399574	26.25097084872316	24.116786045102256
28-29	23.942548790538904	26.270645074342102	26.038505587104815	23.748300548014175
30-31	24.408778045672776	26.0129503488397	25.785896130086673	23.792375475400853
32-33	24.054684572900868	26.096258151732105	25.774248859726157	24.07480841564087
34-35	24.17647923036317	26.19090853054628	25.939056204937405	23.693556034153143
36-37	24.30829949762513	25.991422632907064	25.87195832022869	23.828319549239126
38-39	24.148095925942954	26.157725299611002	25.811185378419705	23.882993396026343
40-41	24.1671352373897	25.957121746631433	25.996767186441833	23.87897582953703
42-43	23.96834229270328	26.034035316723063	25.761690121575093	24.235932268998557
44-45	23.792684594084186	26.088484665917438	25.95878624972483	24.160044490273545
46-47	23.92641175608613	25.942776501137395	25.91627770405673	24.214534038719755
48-49	23.74361378286667	26.219296435951918	25.781939148662552	24.25515063251886
50-51	23.80686027363945	26.2510991057656	25.84072612392862	24.10131449666633
52-53	23.9521476456011	26.101642320223018	25.73630432024211	24.209905713933775
54-55	23.468144047402337	26.029576038714975	25.930261959305973	24.572017954576715
56-57	23.706412803732533	26.111700828021867	25.735294390265878	24.44659197797973
58-59	23.799884355681442	25.95772874854923	25.77290914599386	24.46947774977546
60-61	23.948835253375385	25.89353533576552	25.983878079283794	24.173751331575293
62-63	23.739527322456286	25.786789515806507	26.068371830275776	24.405311331461434
64-65	23.592021757831247	25.734134961055773	25.991233030887912	24.682610250225068
66-67	23.549265427033273	25.84653795057321	25.865361747782618	24.738834874610895
68-69	23.533015589398687	25.70024017556089	25.97736011903024	24.78938411601018
70-71	23.429542398418654	25.655477797968658	26.076078696910166	24.838901106702522
72-73	23.39837255064962	25.831715438897252	25.882854155771422	24.88705785468171
74-75	23.212971001974424	25.878639581774788	26.01113292559687	24.89725649065392
76-77	23.19780738727708	25.493775810539574	26.118099482294628	25.19031731988872
78-79	23.187235997143933	25.648991960448043	26.194393963996625	24.969378078411395
80-81	23.340975732093796	25.680674888871955	26.053387799558216	24.924961579476033
82-83	23.343877379744068	25.570046294755855	26.188295246522426	24.897781078977655
84-85	23.183638400191303	25.707976474546456	25.96773707696564	25.140648048296597
86-87	23.035371055155647	25.70764214498289	26.209300607767826	25.047686192093632
88-89	23.089016279462466	25.364622924705426	26.222581570158948	25.32377922567316
90-91	23.25020021663528	25.22989114573037	26.284159979417147	25.235748658217204
92-93	23.305186925302724	25.2300457283365	26.280957820609462	25.18380952575131
94-95	23.11326350803006	25.169762166134095	26.33497237796421	25.382001947871636
96-97	23.00869193238323	24.94475796556589	26.385137714935166	25.661412387115718
98-99	22.858239562529466	25.12121248909136	26.26956523270231	25.750982715676862
100-101	22.873007104703067	25.171093634235355	26.359442725577125	25.596456535484457
102-103	22.793991251257385	25.05600594554948	26.385201364251547	25.764801438941586
104-105	22.753705687956142	25.189874837035354	26.263085473862596	25.793334001145908
106-107	22.689714319183736	25.1509699891309	26.34331780322633	25.815997888459037
108-109	22.54959202034727	25.259706824053286	26.375918839277407	25.814782316322038
110-111	22.424604730352833	25.594890203133243	26.314258253649157	25.666246812864767
112-113	22.382700690330417	25.677746388122273	26.315210449149916	25.624342472397394
114-115	22.365406227045963	25.834571065113575	26.120736197456257	25.679286510384202
116-117	22.185368313290255	25.7196020519342	26.230435231945588	25.86459440282996
118-119	22.30020087933134	25.603217257568005	26.222779015496645	25.87380284760401
120-121	22.232902376592314	25.74413674354339	26.256997323875233	25.765963555989064
122-123	22.236135902352103	25.852434315787853	26.083222631991482	25.828207149868565
124-125	22.322125255483925	25.823884097197443	25.87812779526063	25.975862852058007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2967.0
1	2360.0
2	1416.5
3	994.0
4	800.5
5	683.0
6	680.5
7	709.0
8	807.0
9	914.0
10	934.5
11	975.5
12	1060.0
13	1184.0
14	1361.0
15	1544.0
16	1763.5
17	2021.0
18	2296.0
19	2671.0
20	3077.5
21	3649.5
22	4546.5
23	5618.0
24	6880.5
25	9082.5
26	13065.5
27	17843.5
28	21724.0
29	26514.5
30	33330.0
31	41031.5
32	50848.5
33	59104.0
34	67372.0
35	77151.5
36	87030.0
37	97836.5
38	107291.5
39	115635.5
40	124095.0
41	134446.5
42	144363.5
43	156590.0
44	170183.5
45	184534.5
46	200282.0
47	212410.0
48	224465.5
49	234085.5
50	240410.0
51	245664.0
52	244206.5
53	242864.0
54	241830.5
55	231773.5
56	213631.0
57	191590.0
58	171267.0
59	150874.5
60	127557.0
61	105744.5
62	85745.0
63	64836.5
64	47018.5
65	34705.0
66	26169.0
67	19865.5
68	14477.0
69	10356.0
70	7568.5
71	5393.5
72	3804.0
73	2757.0
74	2063.5
75	1513.0
76	1037.5
77	712.0
78	493.5
79	324.5
80	219.0
81	162.0
82	123.0
83	99.0
84	83.5
85	62.5
86	45.0
87	43.0
88	40.5
89	33.0
90	27.0
91	17.5
92	11.0
93	11.0
94	12.0
95	15.0
96	11.5
97	11.5
98	11.5
99	9.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1648549113758966
2	0.14514419941905268
3	0.17081999525757308
4	0.14388449226391606
5	0.16854140731519357
6	0.10726035923884047
7	0.1070195328709467
8	0.0951449404232616
9	0.10125822514671884
10-11	0.0880498251230067
12-13	0.07594366886003913
14-15	0.07574915525520184
16-17	0.07547127867686287
18-19	0.07316490307664947
20-21	0.06779262256209614
22-23	0.059530425632817595
24-25	0.06306872073033375
26-27	0.07335015412887545
28-29	0.06843173869227577
30-31	0.06582896140850081
32-33	0.07374844389116131
34-35	0.07127534234394452
36-37	0.07410968344300195
38-39	0.06950619479518644
40-41	0.07441534767917482
42-43	0.07422083407433754
44-45	0.06747769577331199
46-47	0.06475450530559014
48-49	0.05674239729681665
50-51	0.062123940363981264
52-53	0.06458777935858676
54-55	0.06628282648645445
56-57	0.06998784753097398
58-59	0.05723331258521548
60-61	0.06712571877408263
62-63	0.06275379394154959
64-65	0.05663124666548106
66-67	0.06137367360246607
68-69	0.06455072914814157
70-71	0.058900572055249276
72-73	0.05465832295927441
74-75	0.05727962534827198
76-77	0.06673669156440808
78-79	0.06024364218388761
80-81	0.058854259292192776
82-83	0.06314282115122413
84-85	0.06479155551603534
86-87	0.05880794652913629
88-89	0.05785390361017251
90-91	0.06110505957673839
92-93	0.05477873614322129
94-95	0.06435621554330428
96-97	0.05061058746813682
98-99	0.05000852154840239
100-101	0.0476002578694647
102-103	0.04534019503230779
104-105	0.0436173602466062
106-107	0.05498251230066987
108-109	0.05479726124844389
110-111	0.06196647696958919
112-113	0.061957214416977886
114-115	0.0642358023593574
116-117	0.06775557235165096
118-119	0.061114322129349696
120-121	0.0618553263382536
122-123	0.06130883573418697
124-125	0.07234053589424388
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	5398080.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.35038539268907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.69222982565424	29.514927919923167
2	13.343121815396042	11.301727024472388
3	5.815353362037223	7.388473682308396
4	3.0529397244955567	5.171726956521472
5	1.8599623339676727	3.93850608297082
6	1.2979882561629497	3.2982181730211204
7	0.8652365176348908	2.565016998436609
8	0.633679311372212	2.1469250441589596
9	0.49233227979391897	1.8765415611480323
>10	2.685716161527436	20.65194977124569
>50	0.179509868188599	5.185465405678106
>100	0.07850098088156128	5.606761777269011
>500	0.0025941582786159164	0.7444465619838398
>1k	8.354046092071079E-4	0.6093130408623968
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.705021044519533E-5
2	0.0	0.0	0.0	0.0	3.705021044519533E-5
3	0.0	0.0	0.0	0.0	3.705021044519533E-5
4	0.0	0.0	0.0	0.0	3.705021044519533E-5
5	0.0	0.0	0.0	0.0	3.705021044519533E-5
6	0.0	0.0	0.0	0.0	5.5575315667792994E-5
7	0.0	0.0	0.0	0.0	5.5575315667792994E-5
8	0.0	0.0	0.0	0.0	5.5575315667792994E-5
9	0.0	0.0	0.0	0.0	5.5575315667792994E-5
10-11	0.0	0.0	0.0	0.0	5.5575315667792994E-5
12-13	0.0	0.0	0.0	0.0	2.2230126267117198E-4
14-15	0.0	0.0	0.0	0.0	2.7787657833896497E-4
16-17	0.0	0.0	0.0	3.705021044519533E-5	3.149267887841603E-4
18-19	0.0	0.0	0.0	7.410042089039067E-5	3.149267887841603E-4
20-21	0.0	0.0	0.0	1.8525105222597663E-4	3.797646570632521E-4
22-23	9.262552611298832E-6	0.0	0.0	3.0566423617286147E-4	4.075523148971486E-4
24-25	3.705021044519533E-5	0.0	0.0	5.372280514553322E-4	4.075523148971486E-4
26-27	3.705021044519533E-5	0.0	0.0	9.169927085185845E-4	4.4460252534234395E-4
28-29	3.705021044519533E-5	0.0	0.0	0.0031214802300077063	4.4460252534234395E-4
30-31	3.705021044519533E-5	0.0	0.0	0.013134299602821744	4.4460252534234395E-4
32-33	3.705021044519533E-5	0.0	0.0	0.042774467958978005	4.4460252534234395E-4
34-35	3.705021044519533E-5	0.0	0.0	0.08669749244175706	5.001778410101369E-4
36-37	3.705021044519533E-5	0.0	0.0	0.1634747910368131	5.001778410101369E-4
38-39	3.705021044519533E-5	0.0	0.0	0.2932431531211097	5.001778410101369E-4
40-41	3.705021044519533E-5	0.0	0.0	0.4462419971545438	5.094403936214358E-4
42-43	3.705021044519533E-5	0.0	0.0	0.5967029017724821	5.464906040666311E-4
44-45	3.705021044519533E-5	0.0	0.0	0.7737473323848479	5.835408145118265E-4
46-47	3.705021044519533E-5	0.0	0.0	0.9854059221056376	6.205910249570218E-4
48-49	3.705021044519533E-5	0.0	0.0	1.2086704902483847	6.669037880135159E-4
50-51	3.705021044519533E-5	0.0	0.0	1.4502100746932243	7.039539984587112E-4
52-53	3.705021044519533E-5	0.0	0.0	1.6941301351591678	7.410042089039066E-4
54-55	3.705021044519533E-5	0.0	0.0	1.9626700604659435	7.410042089039066E-4
56-57	3.705021044519533E-5	0.0	0.0	2.268093470270911	7.410042089039066E-4
58-59	3.705021044519533E-5	0.0	0.0	2.6013971634358883	7.410042089039066E-4
60-61	3.705021044519533E-5	0.0	0.0	2.961590046831466	7.410042089039066E-4
62-63	3.705021044519533E-5	0.0	0.0	3.336427025905507	7.965795245716996E-4
64-65	3.705021044519533E-5	0.0	0.0	3.7162287331792045	8.614173928507913E-4
66-67	3.705021044519533E-5	0.0	0.0	4.143649223427589	8.706799454620902E-4
68-69	3.705021044519533E-5	0.0	0.0	4.623894051218211	8.706799454620902E-4
70-71	3.705021044519533E-5	0.0	0.0	5.141050151164858	8.706799454620902E-4
72-73	3.705021044519533E-5	0.0	0.0	5.664893814096864	9.540429189637797E-4
74-75	3.705021044519533E-5	0.0	0.0	6.193980081806865	0.0010466684450767681
76-77	3.705021044519533E-5	0.0	0.0	6.774204902483847	0.0010559309976880668
78-79	3.705021044519533E-5	0.0	0.0	7.419638093544371	0.0010651935502993656
80-81	3.705021044519533E-5	0.0	0.0	8.090895281285198	0.0010837186555219633
82-83	3.705021044519533E-5	0.0	0.0	8.794914117612187	0.0010929812081332622
84-85	3.705021044519533E-5	0.0	0.0	9.492291703716877	0.0010929812081332622
86-87	3.705021044519533E-5	0.0	0.0	10.219448396466891	0.0011115063133558599
88-89	3.705021044519533E-5	0.0	0.0	11.008071388345485	0.0011300314185784576
90-91	3.705021044519533E-5	0.0	0.0	11.84875918845219	0.0011300314185784576
92-93	3.705021044519533E-5	0.0	0.0	12.702192260952042	0.0011856067342462505
94-95	3.705021044519533E-5	9.262552611298832E-6	0.0	13.557014716343588	0.0011856067342462505
96-97	3.705021044519533E-5	1.8525105222597663E-5	0.0	14.432557501926611	0.0012504446025253424
98-99	3.705021044519533E-5	1.8525105222597663E-5	0.0	15.358238484794594	0.0012782322603592387
100-101	3.705021044519533E-5	1.8525105222597663E-5	0.0	16.35183250340862	0.0012782322603592387
102-103	3.705021044519533E-5	1.8525105222597663E-5	0.0	17.372287924595412	0.0012782322603592387
104-105	3.705021044519533E-5	1.8525105222597663E-5	0.0	18.40677611298832	0.0012782322603592387
106-107	3.705021044519533E-5	2.7787657833896497E-5	0.0	19.43654039954947	0.0012782322603592387
108-109	3.705021044519533E-5	3.705021044519533E-5	0.0	20.4944906337068	0.0012782322603592387
110-111	3.705021044519533E-5	3.705021044519533E-5	0.0	21.588425884759026	0.0012782322603592387
112-113	3.705021044519533E-5	3.705021044519533E-5	0.0	22.716975294919674	0.0012782322603592387
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9165	0.0	40.24561	1
GTATCAA	13330	0.0	39.650967	1
GTACATG	25925	0.0	34.684128	1
TACATGG	26630	0.0	33.12676	2
CATGGGG	19765	0.0	32.72743	4
ACATGGG	26680	0.0	32.41692	3
TCAACGC	16110	0.0	32.3143	4
TATCAAC	16445	0.0	31.765867	2
CAACGCA	16465	0.0	31.472876	5
AACGCAG	16795	0.0	30.870743	6
ATCAACG	16920	0.0	30.7682	3
ATGGGGG	11770	0.0	27.985146	5
ACGCAGA	18970	0.0	27.045593	7
CGCAGAG	19910	0.0	25.61567	8
GCAGAGT	21750	0.0	22.299467	9
TACCGTA	440	0.0	21.643318	7
AGAGTAC	19200	0.0	20.59404	10-11
ATGGGGA	7810	0.0	20.515408	5
GTACCGT	880	0.0	20.293056	6
CAGAGTA	20445	0.0	20.096684	10-11
>>END_MODULE