FastQCFastQC Report
Mon 15 Aug 2016
SRR2920906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5398080
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102610.19008610468907464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91490.16948618768154602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61290.11354036990930108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109050.050.0090561
GTATCAA174100.045.5464251
TCAACGC223450.034.9625324
TATCAAC224450.034.887642
CAACGCA226600.034.4502335
ATCAACG229100.034.1262863
AACGCAG233150.033.4921076
GTACATG252900.031.7558961
TACATGG261750.030.3028162
CATGGGG196300.029.7879474
ACATGGG260800.029.5901243
ACGCAGA268450.029.0145387
CGCAGAG279650.027.8268418
ATGGGGG112550.025.606445
GCAGAGT301500.025.3182989
AGAGTAC277450.023.37496810-11
AGTACTT164100.022.78809212-13
GAGTACT156100.022.56472612-13
CAGAGTA290900.022.0078610-11
GTAAGTA25050.021.6321222
Produced by FastQC (version 0.11.5)