FastQCFastQC Report
Mon 15 Aug 2016
SRR2920905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5090306
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87900.17268117083727383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77050.15136614576805402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85200.052.314021
GTATCAA141800.048.5165751
ATCAACG181900.037.517883
TCAACGC182750.037.375234
CAACGCA184150.036.9937065
TATCAAC186750.036.6395452
AACGCAG190500.035.9262356
ACGCAGA218100.031.2915427
CGCAGAG225050.030.1894448
GTACATG178850.029.925441
TACATGG181850.028.9487342
ACATGGG187050.027.188583
CATGGGG127400.026.5962644
GCAGAGT251850.026.3640169
AGAGTAC221750.024.94024510-11
GAGTACT142550.023.93763212-13
AGTACTT153100.023.0265112-13
CAGAGTA238800.022.81071910-11
GTACTTT162500.020.87067214-15
TACTTTT197500.017.80472814-15
Produced by FastQC (version 0.11.5)