FastQCFastQC Report
Mon 15 Aug 2016
SRR2920904_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920904_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2650280
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38250.1443243732737673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG52500.027.6824974
TACATGG107650.025.6197322
GTACATG111050.025.5988751
ACATGGG110950.024.7498763
GTATCAA84800.024.1056791
AACGCAG90900.021.9365586
GGTATCA66700.021.7120841
CAACGCA96200.020.8036235
TCAACGC95550.020.7581334
ATCAACG97450.020.2323133
TATCAAC99900.019.9750042
GTATACG2107.061833E-519.8655151
ACGCAGA100050.019.4518457
CGCAGAG99800.019.0813268
ATGGGGA32300.018.9936965
GTATAAT15950.017.935011
ACCGTAT2452.3267427E-417.0028258
GAGTACT37700.016.96579612-13
GTATAGC12300.016.9583661
GCGTAGA7950.016.4921261
Produced by FastQC (version 0.11.5)