##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920904_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2650280
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.039988227658963	31.0	30.0	34.0	22.0	34.0
2	29.139906349517787	31.0	30.0	34.0	22.0	34.0
3	29.29709389196613	31.0	30.0	34.0	22.0	34.0
4	32.638296708272335	37.0	35.0	37.0	23.0	37.0
5	32.75124967927917	37.0	35.0	37.0	25.0	37.0
6	32.71468637276062	37.0	35.0	37.0	24.0	37.0
7	32.63412771480749	37.0	33.0	37.0	23.0	37.0
8	32.510496249452885	37.0	33.0	37.0	22.0	37.0
9	33.84932007184147	39.0	34.0	39.0	19.0	39.0
10-11	33.86895705359434	39.0	34.0	39.0	21.5	39.0
12-13	33.7992746049474	39.0	34.0	39.0	20.0	39.0
14-15	34.879728179664035	40.0	34.0	41.0	19.0	41.0
16-17	34.858670970614426	40.0	34.0	41.0	19.0	41.0
18-19	34.85264330561299	40.0	34.0	41.0	19.0	41.0
20-21	34.87874507599197	40.0	34.0	41.0	20.0	41.0
22-23	34.78941602396728	39.5	34.0	41.0	20.5	41.0
24-25	34.79163918529363	39.0	34.0	41.0	22.0	41.0
26-27	34.70391713328404	39.0	34.0	41.0	20.5	41.0
28-29	34.58001513047678	39.0	34.0	41.0	19.0	41.0
30-31	34.413084843865555	39.0	34.0	41.0	18.0	41.0
32-33	34.25562261345971	39.0	34.0	41.0	16.5	41.0
34-35	34.16514858807371	39.0	33.5	41.0	16.0	41.0
36-37	34.01739929365954	39.0	33.0	41.0	15.5	41.0
38-39	33.87415310835082	39.0	33.0	41.0	14.0	41.0
40-41	33.737020994008176	39.0	33.0	41.0	9.0	41.0
42-43	33.55443707834644	39.0	33.0	41.0	9.0	41.0
44-45	33.40323154534615	38.0	33.0	41.0	8.0	41.0
46-47	33.20064049836244	38.0	32.0	40.5	6.0	41.0
48-49	33.076228738095594	38.0	32.0	40.0	2.0	41.0
50-51	32.89123488838915	38.0	32.0	40.0	2.0	41.0
52-53	32.70008018020737	38.0	32.0	40.0	2.0	41.0
54-55	32.46841616734835	38.0	31.0	40.0	2.0	41.0
56-57	32.215605332266776	38.0	31.0	40.0	2.0	41.0
58-59	31.954791946511314	37.0	31.0	40.0	2.0	41.0
60-61	31.701273450352417	37.0	31.0	40.0	2.0	41.0
62-63	31.402334093001492	36.0	30.0	40.0	2.0	41.0
64-65	31.128739416212625	36.0	30.0	39.0	2.0	41.0
66-67	30.78307442987156	35.5	29.5	39.0	2.0	41.0
68-69	30.46104373877477	35.0	29.0	39.0	2.0	40.0
70-71	30.121818260712075	35.0	29.0	38.0	2.0	40.0
72-73	30.159726708121404	35.0	29.5	38.0	2.0	40.0
74-75	30.145837232292436	35.0	30.0	37.0	2.0	39.5
76-77	29.8674660790558	35.0	30.0	37.0	2.0	39.0
78-79	29.560945824592118	35.0	30.0	36.0	2.0	39.0
80-81	29.267378352475966	35.0	29.0	36.0	2.0	38.0
82-83	29.014130582429026	35.0	29.0	35.5	2.0	37.0
84-85	28.765709660866023	35.0	29.0	35.0	2.0	37.0
86-87	28.5491512217577	34.0	29.0	35.0	2.0	36.0
88-89	28.332888411790453	34.0	29.0	35.0	2.0	36.0
90-91	28.146840711170142	34.0	29.0	35.0	2.0	36.0
92-93	27.977425781426867	34.0	29.0	35.0	2.0	35.5
94-95	27.855237559805	34.0	28.0	35.0	2.0	35.0
96-97	27.75481628356249	34.0	28.0	35.0	2.0	35.0
98-99	27.619847714203782	34.0	27.0	35.0	2.0	35.0
100-101	27.49096623752962	34.0	27.0	35.0	2.0	35.0
102-103	26.193548606185008	32.0	24.5	34.0	2.0	35.0
104-105	27.16709781607981	34.0	26.5	35.0	2.0	35.0
106-107	27.319108169702822	34.0	27.0	35.0	2.0	35.0
108-109	27.25327210709812	34.0	27.0	35.0	2.0	35.0
110-111	27.156458562868828	34.0	26.5	35.0	2.0	35.0
112-113	27.03569151184026	34.0	25.5	35.0	2.0	35.0
114-115	26.92537712996363	34.0	25.0	35.0	2.0	35.0
116-117	26.707059631435172	34.0	25.0	35.0	2.0	35.0
118-119	26.509012255308875	34.0	25.0	35.0	2.0	35.0
120-121	26.315972463286897	34.0	24.5	35.0	2.0	35.0
122-123	26.014396592058198	34.0	23.5	35.0	2.0	35.0
124-125	24.74809793682177	32.5	10.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	134301.0
3	18815.0
4	12258.0
5	12601.0
6	16831.0
7	17064.0
8	15314.0
9	16008.0
10	17149.0
11	18381.0
12	18448.0
13	18617.0
14	18545.0
15	16701.0
16	13946.0
17	12637.0
18	12251.0
19	11978.0
20	12205.0
21	12924.0
22	15033.0
23	18874.0
24	25308.0
25	31284.0
26	35263.0
27	38776.0
28	45135.0
29	52781.0
30	61750.0
31	74574.0
32	91178.0
33	114826.0
34	151400.0
35	217398.0
36	378321.0
37	626004.0
38	244992.0
39	409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.355143376687586	19.454757735409185	12.56321995915672	24.62687892874651
2	15.75716487275591	21.490763645928045	38.811355564675644	23.9407159166404
3	18.78897864653401	25.3639456000883	29.74989028617825	26.09718546719944
4	12.50512985427813	17.476950610556578	36.80163127476595	33.21628826039934
5	13.185082315337635	38.62875053202387	34.65590245989693	13.53026469274157
6	31.519762177720782	37.521261787708866	17.220206583552265	13.738769451018094
7	26.68524598585474	33.711681098501614	21.700483646522535	17.902589269121112
8	24.73639365214613	36.524105090535244	20.52069382535319	18.218807431965427
9	25.250509631150557	16.86245492450811	20.074160444766257	37.81287499957507
10-11	23.9357299735881	27.4861827072018	28.35284500556015	20.22524231364995
12-13	24.44030241747818	25.376563306268974	27.911763245397285	22.271371030855562
14-15	22.117286857330154	26.897417897727166	25.25773254270557	25.727562702237112
16-17	21.915633057933004	29.261947382859827	26.492090587380773	22.330328971826393
18-19	21.982725417666686	28.532797086717288	27.918927879336202	21.565549616279824
20-21	23.00398123863946	27.81554450159378	27.465354841486743	21.71511941828002
22-23	22.60648258614805	27.61787309679933	27.171389242102528	22.60425507495009
24-25	22.234700635134228	27.717820985934317	27.461928569864387	22.585549809067068
26-27	22.241947765092636	27.664737180059635	27.509372569772943	22.583942485074786
28-29	22.240352727372958	27.92374690897228	27.579632312600367	22.256268051054395
30-31	22.524237936252174	27.881513281662233	27.37352427286191	22.22072450922368
32-33	22.133876085275745	28.048047140876037	27.352790965294776	22.46528580855344
34-35	22.360670371147943	27.90053256323622	27.40580457678139	22.332992488834446
36-37	22.41557242038693	27.72920264960279	27.359429599818142	22.495795330192134
38-39	22.247218672877263	27.958737732104506	27.329861771893416	22.46418182312481
40-41	22.405361316804054	27.912756822481434	27.44540693217734	22.236474928537174
42-43	22.231563882702897	27.88758162283712	27.418060914213537	22.462793580246448
44-45	22.322749266027557	27.976437365450973	27.30109398755225	22.39971938096922
46-47	22.27850529182935	27.903823158393926	27.19871158085827	22.618959968918457
48-49	22.07541805900113	27.867007157017575	27.38753190681266	22.670042877168633
50-51	21.936900218117948	28.09562680498549	27.219983485409276	22.747489491487283
52-53	22.1095817948032	27.93994630538496	27.022236955948404	22.928234943863433
54-55	22.16129148576707	27.82497408393238	27.049276115287658	22.96445831501289
56-57	22.10893157835415	27.872160425895288	27.042115460170397	22.976792535580167
58-59	22.037407202626028	27.59297009926891	27.217108525664052	23.15251417244101
60-61	22.070000220885206	27.49552613062547	27.25343972071048	23.181033927778845
62-63	22.01092825200827	27.38343801962434	27.303941631414645	23.301692096952745
64-65	21.938185602514725	27.36031505034791	27.30126765027403	23.400231696863333
66-67	21.99071395332813	27.51410888215367	27.14406398103147	23.35111318348673
68-69	22.11222499066453	27.22768464123668	27.220076564063973	23.44001380403481
70-71	21.8967758133617	27.02168366744977	27.545629334161426	23.535911185027103
72-73	21.978145193700012	26.88117768068138	27.593122846783984	23.54755427883462
74-75	21.749181253465125	27.0819777223924	27.552734563402193	23.61610646074028
76-77	21.766576700941243	26.92877553979435	27.727403851962833	23.577243907301572
78-79	21.92756154995373	26.926370364049824	27.67319295588157	23.47287513011488
80-81	21.856853356288443	26.772947325002665	27.728114065763755	23.642085252945137
82-83	21.88608575897362	26.68582825739963	27.726953939500344	23.701132044126403
84-85	21.9672548954828	26.6607450478811	27.588236682257328	23.783763374378772
86-87	21.91383258651183	26.555332781232238	27.707458405059597	23.823376227196334
88-89	21.97733679817903	26.420027341725127	27.715871074818242	23.886764785277602
90-91	21.957588255859875	26.426599501775648	27.752636670006787	23.863175572357687
92-93	21.998696004427696	26.355943977269554	27.730198257161216	23.915161761141537
94-95	21.74032882157669	26.3163925983429	27.850739742826853	24.092538837253556
96-97	21.797148524240868	26.29509746405454	27.805059810319406	24.10269420138519
98-99	21.725982839384248	26.36796790765523	27.69110514123833	24.214944111722193
100-101	21.642760176554173	26.55230485342772	27.712753668270835	24.09218130174727
102-103	21.484009512392667	26.576884447909947	27.778372349507762	24.160733690189623
104-105	21.33909172294724	26.60439044176784	27.72179133708531	24.334726498199608
106-107	21.209833065025204	26.75178653948962	27.75943332115124	24.27894707433394
108-109	21.15556397898137	26.809492814960407	27.712011712924618	24.322931493133606
110-111	21.1319835017791	26.955539076800395	27.66868992889635	24.24378749252415
112-113	21.110229697748633	27.037908661579053	27.61462610873075	24.237235531941565
114-115	21.01002885073924	27.14521916329458	27.542261367836918	24.302490618129262
116-117	20.96762378585421	27.05517443976734	27.60071878336966	24.37648299100879
118-119	21.064820889027747	26.925743821896177	27.538867708676484	24.470567580399592
120-121	21.017586929361173	27.087057924463686	27.578090399059697	24.317264747115445
122-123	21.152512718533277	27.004199017932333	27.443356299593503	24.399931963940887
124-125	21.070263728524914	27.12330396513199	27.325265810070526	24.481166496272568
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3091.0
1	2114.5
2	931.5
3	651.0
4	506.0
5	417.0
6	379.0
7	394.5
8	457.5
9	519.0
10	556.5
11	584.5
12	627.5
13	702.0
14	813.5
15	954.5
16	1167.5
17	1399.5
18	1682.5
19	2067.5
20	2549.5
21	3303.5
22	4286.5
23	5516.0
24	7192.5
25	9327.5
26	12694.5
27	16928.5
28	20408.0
29	24697.0
30	29866.0
31	34901.0
32	40682.0
33	46415.5
34	52031.5
35	57237.5
36	63863.0
37	70845.0
38	75394.5
39	78061.0
40	81565.5
41	86341.5
42	89991.0
43	94350.0
44	98689.0
45	101297.5
46	104075.5
47	106407.5
48	107772.0
49	108559.5
50	109568.0
51	107480.0
52	99698.0
53	93579.0
54	88231.5
55	80594.0
56	72328.0
57	63759.0
58	57191.0
59	50593.5
60	41890.0
61	33126.5
62	25864.0
63	19270.5
64	13778.5
65	10283.0
66	7592.0
67	5628.5
68	4168.5
69	3011.0
70	2238.0
71	1678.0
72	1222.5
73	904.0
74	711.0
75	554.5
76	416.0
77	283.0
78	192.5
79	143.5
80	105.5
81	80.5
82	66.0
83	46.5
84	33.0
85	29.5
86	28.5
87	22.0
88	16.5
89	14.0
90	10.5
91	9.5
92	12.5
93	10.0
94	7.5
95	11.0
96	11.0
97	12.0
98	13.0
99	12.5
100	18.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17194409647282552
2	0.1549270265783238
3	0.17964139638075977
4	0.15115384034894425
5	0.17816985375130176
6	0.11036569720935145
7	0.10912054575365623
8	0.09742366844257964
9	0.10478138158986974
10-11	0.09132997268213171
12-13	0.07872753067600405
14-15	0.07882186033173853
16-17	0.0782747483284785
18-19	0.07531279713841557
20-21	0.0701058001418718
22-23	0.05976726987337187
24-25	0.06437055707321492
26-27	0.07521846748268107
28-29	0.07127548787297945
30-31	0.06782302247309718
32-33	0.07725598804654603
34-35	0.07380352264666375
36-37	0.07674660790557979
38-39	0.07184146580738639
40-41	0.07735031770228051
42-43	0.07725598804654603
44-45	0.06978507931237454
46-47	0.06652127322396124
48-49	0.05852211841767662
50-51	0.06463468010927148
52-53	0.06729477640098404
54-55	0.06891724647961725
56-57	0.07280362829587816
58-59	0.059069230420936654
60-61	0.06963415186319936
62-63	0.06535158549285358
64-65	0.058880571109467685
66-67	0.0634838583093107
68-69	0.06712498302066197
70-71	0.061408605883151966
72-73	0.05586202212596405
74-75	0.05910696228323046
76-77	0.07033219131563458
78-79	0.06259715954540652
80-81	0.06159726519462095
82-83	0.06612508866987639
84-85	0.06763436316162821
86-87	0.06076716422415744
88-89	0.05965407428649048
90-91	0.06316313747981345
92-93	0.05652232971610547
94-95	0.0676154972304813
96-97	0.0530509983850763
98-99	0.05373017190636461
100-101	0.05086255037203616
102-103	0.04959853298519402
104-105	0.048126990355736
106-107	0.05976726987337187
108-109	0.059691806148784284
110-111	0.06610622273872949
112-113	0.06625715018790466
114-115	0.06870972123700138
116-117	0.07229424815491194
118-119	0.06461581417812458
120-121	0.06712498302066197
122-123	0.0635593220338983
124-125	0.0757467135547942
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2650280.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.0526575174566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.45734754928483	36.67492181571334
2	13.85437857390301	14.423144460491244
3	5.947420613393157	9.28737144903647
4	3.2500544138526126	6.7669587726947285
5	1.892266086516663	4.924873926667488
6	1.2267568417859578	3.8313572245608696
7	0.8165450266161387	2.9752337032622647
8	0.5483933322252708	2.2836264245743054
9	0.3942183748281636	1.846810264681686
>10	1.5476507051235864	12.781988475208456
>50	0.04485748585050951	1.6005734890256929
>100	0.01857992263506532	1.6796097122526876
>500	0.001020715989945401	0.3648959794490412
>1k	5.103579949619291E-4	0.5586343023818333
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3825	0.1443243732737673	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.773186229379537E-5
7	0.0	0.0	0.0	0.0	3.773186229379537E-5
8	0.0	0.0	0.0	0.0	3.773186229379537E-5
9	0.0	0.0	0.0	0.0	3.773186229379537E-5
10-11	0.0	0.0	0.0	0.0	3.773186229379537E-5
12-13	0.0	0.0	0.0	0.0	7.546372458759074E-5
14-15	1.8865931146897685E-5	0.0	0.0	1.8865931146897685E-5	9.432965573448842E-5
16-17	3.773186229379537E-5	0.0	0.0	7.546372458759074E-5	1.5092744917518148E-4
18-19	3.773186229379537E-5	0.0	0.0	1.3206151802828378E-4	1.8865931146897687E-4
20-21	3.773186229379537E-5	0.0	0.0	2.2639117376277223E-4	2.2639117376277223E-4
22-23	3.773186229379537E-5	0.0	0.0	5.093801409662375E-4	2.2639117376277223E-4
24-25	3.773186229379537E-5	0.0	0.0	8.301009704634983E-4	2.2639117376277223E-4
26-27	3.773186229379537E-5	0.0	0.0	0.0017167997343676895	3.207208294972606E-4
28-29	3.773186229379537E-5	0.0	0.0	0.005357924445718943	3.3958676064415835E-4
30-31	3.773186229379537E-5	0.0	0.0	0.019790361773095674	3.3958676064415835E-4
32-33	3.773186229379537E-5	0.0	0.0	0.05978613580451877	3.3958676064415835E-4
34-35	3.773186229379537E-5	0.0	0.0	0.12809967248743528	3.7731862293795374E-4
36-37	3.773186229379537E-5	0.0	0.0	0.24495525001131957	3.7731862293795374E-4
38-39	3.773186229379537E-5	0.0	0.0	0.41972923615617974	3.7731862293795374E-4
40-41	3.773186229379537E-5	0.0	0.0	0.6150670872511583	3.7731862293795374E-4
42-43	3.773186229379537E-5	0.0	0.0	0.7942745672155395	4.1505048523174913E-4
44-45	3.773186229379537E-5	0.0	0.0	1.020609143184871	4.5278234752554446E-4
46-47	3.773186229379537E-5	0.0	0.0	1.2940896810903	4.5278234752554446E-4
48-49	3.773186229379537E-5	0.0	0.0	1.5728338137857132	4.905142098193398E-4
50-51	3.773186229379537E-5	0.0	0.0	1.8527665001433813	4.905142098193398E-4
52-53	3.773186229379537E-5	0.0	0.0	2.1356611376911117	4.905142098193398E-4
54-55	3.773186229379537E-5	0.0	0.0	2.456872481398192	5.282460721131352E-4
56-57	3.773186229379537E-5	0.0	0.0	2.8379454246343783	5.282460721131352E-4
58-59	3.773186229379537E-5	0.0	0.0	3.238544606607604	5.282460721131352E-4
60-61	3.773186229379537E-5	0.0	0.0	3.644746970131458	5.282460721131352E-4
62-63	3.773186229379537E-5	0.0	0.0	4.052873658632295	5.659779344069306E-4
64-65	3.773186229379537E-5	0.0	0.0	4.484695956653637	5.659779344069306E-4
66-67	3.773186229379537E-5	0.0	0.0	4.9892652851774155	5.659779344069306E-4
68-69	3.773186229379537E-5	0.0	0.0	5.5510172510074405	5.659779344069306E-4
70-71	3.773186229379537E-5	0.0	0.0	6.146803356626469	5.659779344069306E-4
72-73	3.773186229379537E-5	0.0	0.0	6.7211011666691824	5.848438655538283E-4
74-75	3.773186229379537E-5	0.0	0.0	7.307699563819671	6.037097967007259E-4
76-77	3.773186229379537E-5	0.0	0.0	7.969252305416786	6.037097967007259E-4
78-79	3.773186229379537E-5	0.0	0.0	8.699552500113196	6.037097967007259E-4
80-81	3.773186229379537E-5	0.0	0.0	9.45469912613007	6.414416589945214E-4
82-83	3.773186229379537E-5	0.0	0.0	10.238238978523023	6.60307590141419E-4
84-85	3.773186229379537E-5	0.0	0.0	11.011478032509773	6.791735212883167E-4
86-87	3.773186229379537E-5	0.0	0.0	11.84223931056341	6.980394524352144E-4
88-89	3.773186229379537E-5	0.0	0.0	12.74320071841466	7.16905383582112E-4
90-91	3.773186229379537E-5	0.0	0.0	13.680592239310563	7.16905383582112E-4
92-93	3.773186229379537E-5	0.0	0.0	14.638358965845118	7.546372458759075E-4
94-95	3.773186229379537E-5	0.0	0.0	15.58944715275367	7.546372458759075E-4
96-97	3.773186229379537E-5	0.0	0.0	16.56860784520881	8.301009704634983E-4
98-99	3.773186229379537E-5	0.0	0.0	17.600366753701493	8.678328327572936E-4
100-101	3.773186229379537E-5	0.0	0.0	18.717795855532245	8.678328327572936E-4
102-103	3.773186229379537E-5	0.0	0.0	19.83690402523507	8.678328327572936E-4
104-105	3.773186229379537E-5	0.0	0.0	20.947880978613583	8.678328327572936E-4
106-107	3.773186229379537E-5	0.0	0.0	22.045897037294175	8.678328327572936E-4
108-109	3.773186229379537E-5	0.0	0.0	23.183739076625866	8.678328327572936E-4
110-111	3.773186229379537E-5	0.0	0.0	24.369217592103475	8.678328327572936E-4
112-113	3.773186229379537E-5	0.0	0.0	25.583277993268638	9.055646950510889E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	5250	0.0	27.682497	4
TACATGG	10765	0.0	25.619732	2
GTACATG	11105	0.0	25.598875	1
ACATGGG	11095	0.0	24.749876	3
GTATCAA	8480	0.0	24.105679	1
AACGCAG	9090	0.0	21.936558	6
GGTATCA	6670	0.0	21.712084	1
CAACGCA	9620	0.0	20.803623	5
TCAACGC	9555	0.0	20.758133	4
ATCAACG	9745	0.0	20.232313	3
TATCAAC	9990	0.0	19.975004	2
GTATACG	210	7.061833E-5	19.865515	1
ACGCAGA	10005	0.0	19.451845	7
CGCAGAG	9980	0.0	19.081326	8
ATGGGGA	3230	0.0	18.993696	5
GTATAAT	1595	0.0	17.93501	1
ACCGTAT	245	2.3267427E-4	17.002825	8
GAGTACT	3770	0.0	16.965796	12-13
GTATAGC	1230	0.0	16.958366	1
GCGTAGA	795	0.0	16.492126	1
>>END_MODULE