FastQCFastQC Report
Mon 15 Aug 2016
SRR2920904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2650280
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68420.25816140181414793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57150.21563759300904056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41010.15473836726685483No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT30980.11689330938617806No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77650.036.6554221
GTATCAA110300.034.9476781
ATCAACG135500.027.9941043
TCAACGC135200.027.8800434
TATCAAC136300.027.8740122
AACGCAG136500.027.6860856
CAACGCA136450.027.624645
CATGGGG53650.026.7493824
TACATGG114650.025.0350022
GTACATG118850.024.6013071
ACGCAGA153900.024.435267
ACATGGG114250.024.3924873
CGCAGAG155000.024.181428
GAGTACT93250.023.7688312-13
AGTACTT101900.021.69276812-13
GCAGAGT169450.021.6641249
GTATAGG6100.021.5205921
GTACTTT103600.021.19321814-15
AGAGTAC155700.019.92910810-11
ATGGGGA35100.019.84935
Produced by FastQC (version 0.11.5)