##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920904_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2650280
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11921683746623	31.0	31.0	34.0	28.0	34.0
2	31.218262975987443	33.0	31.0	34.0	28.0	34.0
3	31.308080278310218	33.0	31.0	34.0	28.0	34.0
4	34.85576052341639	37.0	35.0	37.0	32.0	37.0
5	34.585180056446866	37.0	35.0	37.0	31.0	37.0
6	34.28015379507071	37.0	35.0	37.0	30.0	37.0
7	34.17355713358589	37.0	35.0	37.0	30.0	37.0
8	34.16993600676155	37.0	35.0	37.0	30.0	37.0
9	35.704825527868756	39.0	35.0	39.0	30.0	39.0
10-11	35.70069879408968	39.0	36.0	39.0	30.0	39.0
12-13	35.61860520397845	39.0	35.0	39.0	30.0	39.0
14-15	36.83302122794573	40.0	37.0	41.0	30.0	41.0
16-17	36.85561280317551	40.0	37.0	41.0	30.0	41.0
18-19	36.90840948880873	40.0	37.0	41.0	30.0	41.0
20-21	36.90557224142355	40.0	37.0	41.0	30.0	41.0
22-23	36.86668144497939	40.0	37.0	41.0	30.0	41.0
24-25	36.8573990295365	40.0	37.0	41.0	30.0	41.0
26-27	36.73549870202394	40.0	37.0	41.0	30.0	41.0
28-29	36.62201088186909	40.0	36.5	41.0	30.0	41.0
30-31	36.46479900236956	40.0	36.0	41.0	30.0	41.0
32-33	36.29784664261889	40.0	36.0	41.0	29.0	41.0
34-35	36.172511961000346	40.0	36.0	41.0	28.5	41.0
36-37	36.04084266568061	40.0	35.0	41.0	28.0	41.0
38-39	35.90406504218423	40.0	35.0	41.0	27.0	41.0
40-41	35.76406964547142	40.0	35.0	41.0	27.0	41.0
42-43	35.62345733281012	40.0	35.0	41.0	26.0	41.0
44-45	35.42007391671824	40.0	35.0	41.0	25.5	41.0
46-47	35.19325165642876	40.0	35.0	41.0	24.0	41.0
48-49	34.93007040765504	39.0	35.0	41.0	23.5	41.0
50-51	34.751344197594214	39.0	35.0	41.0	23.0	41.0
52-53	34.56888819294565	39.0	34.0	41.0	22.0	41.0
54-55	34.383950186395396	39.0	34.0	41.0	21.5	41.0
56-57	34.15045617821514	39.0	34.0	41.0	20.0	41.0
58-59	33.93730511493125	38.0	34.0	41.0	19.0	41.0
60-61	33.693601430792214	38.0	33.0	40.0	18.0	41.0
62-63	33.41465392335904	37.5	33.0	40.0	16.0	41.0
64-65	33.11668295425389	37.0	33.0	40.0	13.0	41.0
66-67	32.79185463422732	36.5	32.5	39.5	11.5	41.0
68-69	32.43236525951975	36.0	32.0	39.0	8.0	41.0
70-71	32.08822011259188	35.5	32.0	39.0	7.5	41.0
72-73	31.70987593763678	35.0	32.0	38.5	6.5	40.0
74-75	31.36577908749264	35.0	31.0	37.0	2.0	39.5
76-77	31.133778883740586	35.0	31.0	37.0	2.0	39.0
78-79	30.962433780581677	35.0	31.0	36.5	2.0	39.0
80-81	30.683600600691246	35.0	31.0	36.0	2.0	38.5
82-83	30.43385246087206	35.0	31.0	36.0	2.0	37.0
84-85	30.162579048251505	35.0	31.0	35.0	2.0	37.0
86-87	29.97438365002943	35.0	31.0	35.0	2.0	36.5
88-89	29.801652089590533	35.0	31.0	35.0	2.0	36.0
90-91	29.625196205683928	35.0	31.0	35.0	2.0	36.0
92-93	29.49613550266387	35.0	31.0	35.0	2.0	35.5
94-95	29.395320871756947	35.0	30.0	35.0	2.0	35.0
96-97	29.31854200310911	35.0	30.0	35.0	2.0	35.0
98-99	29.2091541271111	34.5	30.0	35.0	2.0	35.0
100-101	29.107554862127778	34.0	30.0	35.0	2.0	35.0
102-103	27.725534660488705	32.5	27.5	34.5	2.0	35.0
104-105	28.758376662088537	34.0	29.5	35.0	2.0	35.0
106-107	28.921702423894835	34.0	30.0	35.0	2.0	35.0
108-109	29.00120591031891	34.5	30.0	35.0	2.0	35.0
110-111	29.150045466894063	35.0	31.0	35.0	2.0	35.0
112-113	29.105127194107794	35.0	31.0	35.0	2.0	35.0
114-115	29.000728602260892	35.0	31.0	35.0	2.0	35.0
116-117	28.866761059208837	35.0	31.0	35.0	2.0	35.0
118-119	28.74686410492476	35.0	30.5	35.0	2.0	35.0
120-121	28.540040108969617	35.0	30.0	35.0	2.0	35.0
122-123	28.324889256984168	35.0	30.0	35.0	2.0	35.0
124-125	27.59126167801138	34.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	49752.0
3	20020.0
4	7514.0
5	6604.0
6	8123.0
7	9781.0
8	10947.0
9	11975.0
10	13778.0
11	15997.0
12	17152.0
13	20347.0
14	25205.0
15	22960.0
16	14057.0
17	12090.0
18	11565.0
19	10794.0
20	10865.0
21	11196.0
22	11564.0
23	12376.0
24	16071.0
25	21214.0
26	26835.0
27	32173.0
28	38454.0
29	46652.0
30	55886.0
31	68225.0
32	84630.0
33	107511.0
34	143766.0
35	207092.0
36	377458.0
37	737173.0
38	351378.0
39	1100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.229438686484166	19.866563903274333	13.150512316858162	24.753485093383336
2	16.033934236379686	21.38587439487379	38.43906588022114	24.141125488525383
3	18.705980900059238	25.10774373931713	30.632954378233162	25.553320982390478
4	12.893283832984242	17.3695965273787	36.7528772172241	32.984242422412954
5	13.044098925463748	38.06319872897086	35.101907789962965	13.790794555602426
6	31.663382110874522	37.4898065509899	16.94351319107809	13.903298147057491
7	26.68081760946946	33.1986175656126	21.841315947098522	18.279248877819416
8	25.359813362738816	35.864499295430754	20.397487250202463	18.378200091627967
9	25.73348413028162	15.430120369626113	20.259094516402566	38.5773009836897
10-11	24.420408095493983	26.556377284555886	28.514514137529208	20.50870048242092
12-13	25.18210920250075	24.263685596664956	28.393129455557354	22.161075745276936
14-15	22.513436370985364	25.730617414502937	26.15026695364136	25.605679260870335
16-17	21.872540949732624	28.456898663257917	27.20486584837022	22.465694538639237
18-19	21.859144287829906	27.251652654890634	29.386460835211555	21.502742222067905
20-21	22.72295940885186	26.566343117181447	29.087678004578795	21.623019469387902
22-23	22.314213010815866	26.47600685760951	28.860565839579788	22.34921429199484
24-25	21.98814054661714	26.752073196756093	29.118209055366446	22.141577201260322
26-27	21.98827836229151	26.716141421924995	29.237772790721106	22.057807425062386
28-29	22.018671986604804	26.823296151504273	29.303743095744355	21.854288766146563
30-31	22.36172518262712	26.690134560199635	29.106621934650274	21.841518322522973
32-33	21.976392636156888	26.825455438114776	29.09507495301226	22.103076972716075
34-35	22.086464675740483	26.678490102354047	29.199583972055482	22.035461249849988
36-37	22.214789757589905	26.55579987293316	29.06671914888237	22.162691220594567
38-39	22.141441619261947	26.67633065013751	29.010952556533738	22.17127517406681
40-41	22.24426487846273	26.613239696179946	29.08917953828424	22.05331588707308
42-43	22.295031110986987	26.506477886045516	28.923896113796733	22.274594889170764
44-45	22.25245549984414	26.692828725253477	28.757746910481906	22.29696886442048
46-47	22.25699271932963	26.634263683146674	28.464149151846435	22.644594445677264
48-49	22.16358470625064	26.742229559459453	28.439715201308474	22.65447053298143
50-51	22.017073167970434	27.034226716666243	28.21589885463127	22.732801260732053
52-53	22.133321257645182	27.05810764532572	28.025049505604343	22.78352159142475
54-55	22.12081391987453	26.99764585473437	27.899112157397898	22.98242806799321
56-57	22.083349765927522	27.08045605685294	27.780361914599442	23.05583226262009
58-59	21.85311412900319	26.972865388142463	28.037256031150726	23.13676445170362
60-61	22.003565226530768	27.04120729356208	27.905152973201552	23.050074506705602
62-63	21.80881026161084	27.013438048372144	27.956201748676733	23.22154994134028
64-65	21.6889231053723	27.026185044503443	27.95569091193847	23.329200938185785
66-67	21.79662548932103	27.189106008470716	27.722842152040737	23.29142635016752
68-69	21.9468962427954	26.898141002442006	27.734974823283714	23.41998793147888
70-71	21.756318485163657	26.809389533874445	27.8855283556315	23.548763625330395
72-73	21.9343475057819	26.702282718308247	27.697131845437582	23.666237930472274
74-75	21.71449607369976	26.923926741767996	27.537971909362913	23.82360527516933
76-77	21.70808695099247	26.831468535294995	27.67600043699276	23.784444076719776
78-79	21.841490724296612	26.959751319520603	27.474879482652824	23.72387847352996
80-81	21.644353872425537	26.913211249799513	27.4294906267395	24.012944251035446
82-83	21.671486586208253	26.83250812612585	27.426181833139275	24.06982345452662
84-85	21.729048629079934	26.803006742471112	27.237862448154974	24.230082180293977
86-87	21.645249464396976	26.71871302810722	27.28535593212663	24.35068157536917
88-89	21.599946863924284	26.7506742488727	27.223709860421568	24.42566902678145
90-91	21.534101190239213	26.745768142133592	27.250574024705188	24.46955664292201
92-93	21.557232751870337	26.695227223411983	27.16931611272017	24.57822391199751
94-95	21.406906300903785	26.683323814154686	27.205680487306473	24.704089397635055
96-97	21.44158538693129	26.583171755030943	27.199477565585443	24.775765292452327
98-99	21.410798731967223	26.661085390320466	27.021019222572356	24.907096655139956
100-101	21.258594474402535	26.820137873880157	26.939655741744794	24.981611909972514
102-103	21.219460140023244	26.708676281966003	26.984167421016846	25.087696156993903
104-105	21.125172518214054	26.87759720687941	26.86878616043085	25.128444114475677
106-107	21.023840879674875	26.990607795265788	26.860630096563487	25.12492122849585
108-109	20.88762879536766	27.09224132384288	26.743057215738837	25.277072665050625
110-111	20.839553534231356	27.269989675347023	26.59634003127585	25.29411675914577
112-113	20.770758758931006	27.390909111491986	26.519600851006665	25.318731278570343
114-115	20.676617962972816	27.539340864350002	26.412122227517603	25.37191894515957
116-117	20.647146553667735	27.518378520643466	26.414865447437858	25.419609478250944
118-119	20.64889403396024	27.518086254823658	26.319203295428263	25.513816415787836
120-121	20.552560507054636	27.761128138079755	26.234643891091547	25.451667463774065
122-123	20.488270645087166	27.838497851696705	25.99696026497313	25.676271238243004
124-125	20.33135158294458	27.98573275555496	25.980783441967066	25.702132219533397
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	125.0
1	119.5
2	243.5
3	446.5
4	333.5
5	189.5
6	347.5
7	831.5
8	1371.5
9	1466.5
10	1179.5
11	934.0
12	945.0
13	1081.0
14	1216.5
15	1349.0
16	1496.0
17	1679.5
18	2012.5
19	2385.5
20	2789.0
21	3366.0
22	4151.5
23	5352.0
24	6855.5
25	8823.5
26	12138.0
27	16374.0
28	20091.5
29	24416.5
30	29570.0
31	34994.5
32	41023.5
33	46734.5
34	52454.0
35	57203.0
36	63553.0
37	70362.5
38	74451.0
39	77138.0
40	81126.0
41	86059.5
42	89317.5
43	93935.0
44	98489.0
45	101885.0
46	105220.0
47	107112.5
48	108408.5
49	109594.5
50	111563.0
51	109755.5
52	101343.5
53	94445.0
54	88932.5
55	81539.5
56	73250.0
57	64539.0
58	57754.5
59	50833.5
60	41682.5
61	32471.0
62	24928.5
63	18337.0
64	12713.0
65	9216.0
66	6797.5
67	4932.5
68	3585.0
69	2520.0
70	1779.5
71	1312.5
72	960.0
73	698.5
74	512.0
75	372.5
76	255.0
77	163.5
78	117.0
79	82.0
80	47.5
81	31.0
82	22.5
83	14.0
84	12.0
85	7.5
86	8.0
87	9.0
88	6.5
89	4.5
90	2.5
91	3.0
92	3.5
93	3.5
94	4.5
95	9.5
96	10.5
97	5.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22707034728406053
2	0.008036886668578415
3	3.7731862293795374E-4
4	0.0023771073245091082
5	0.08855668080353774
6	0.05735243068656897
7	0.028336628582640324
8	0.016375628235507193
9	0.01354573856347254
10-11	0.011772341035664156
12-13	0.01354573856347254
14-15	0.021469429645169565
16-17	0.02373334138279729
18-19	0.022790044825452407
20-21	0.017111399550236203
22-23	0.014224912084760856
24-25	0.012319453038924188
26-27	0.01165914544878277
28-29	0.014130582429026367
30-31	0.01043285992423442
32-33	0.014583364776551911
34-35	0.01728119293055828
36-37	0.018620674041988014
38-39	0.02184674826810752
40-41	0.023374888691006235
42-43	0.021620357094344747
44-45	0.019394177219010822
46-47	0.019771495841948775
48-49	0.018054696107581085
50-51	0.01475315815687399
52-53	0.012357184901217984
54-55	0.01833768507478455
56-57	0.02137509998943508
58-59	0.0171302654813831
60-61	0.019563970599332903
62-63	0.025525604841752567
64-65	0.018299953212490755
66-67	0.021224172540259895
68-69	0.015507795402749897
70-71	0.016451091960094782
72-73	0.023846536969678676
74-75	0.014847487812608478
76-77	0.013225017733975278
78-79	0.0171302654813831
80-81	0.017167997343676897
82-83	0.01280996724874353
84-85	0.016432226028947887
86-87	0.017469852242027257
88-89	0.018092427969874883
90-91	0.013225017733975278
92-93	0.013394811114297356
94-95	0.013828727530676004
96-97	0.014262643947054652
98-99	0.013149554009387688
100-101	0.01762077969120244
102-103	0.0244691126975263
104-105	0.007452042803024586
106-107	0.007923691081697028
108-109	0.014658828501139504
110-111	0.011772341035664156
112-113	0.00913111067509848
114-115	0.006527612176826599
116-117	0.004056175196583002
118-119	0.0023771073245091082
120-121	0.0027355600163001645
122-123	0.001848861252395973
124-125	5.093801409662375E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2650280.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.774189025408866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.9878799738817	33.16053709282916
2	14.730924026316758	14.369777459570212
3	6.4946993957900325	9.503210879804154
4	3.550158720465848	6.926244500088198
5	2.10371214212418	5.130342683750627
6	1.2977536198984987	3.7978128219202794
7	0.8872831249526002	3.029356039884552
8	0.6321313764036169	2.4665356193281514
9	0.4310437716244361	1.8921429355891874
>10	1.8033655252054765	14.036836719358892
>50	0.05386150196819104	1.7797923451743474
>100	0.024714660975979116	2.1790061054410415
>500	0.001699610270028695	0.568297387237865
>1k	6.180400981780121E-4	0.6869532997315634
>5k	1.5451002454450302E-4	0.47315411029176907
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6842	0.25816140181414793	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5715	0.21563759300904056	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4101	0.15473836726685483	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	3098	0.11689330938617806	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	5.659779344069306E-5
14-15	0.0	0.0	0.0	0.0	1.1319558688138612E-4
16-17	0.0	0.0	0.0	5.659779344069306E-5	1.6979338032207917E-4
18-19	0.0	0.0	0.0	1.3206151802828378E-4	1.8865931146897687E-4
20-21	0.0	0.0	0.0	2.2639117376277223E-4	3.3958676064415835E-4
22-23	0.0	0.0	0.0	5.471120032600329E-4	3.7731862293795374E-4
24-25	0.0	0.0	0.0	8.489669016103959E-4	3.7731862293795374E-4
26-27	0.0	0.0	0.0	0.0017922634589552801	4.3391641637864674E-4
28-29	0.0	0.0	0.0	0.005565449688334818	4.5278234752554446E-4
30-31	0.0	0.0	0.0	0.020148814464886727	4.5278234752554446E-4
32-33	0.0	0.0	0.0	0.060635102706129165	4.5278234752554446E-4
34-35	0.0	0.0	0.0	0.1296466788414809	4.5278234752554446E-4
36-37	0.0	0.0	0.0	0.24769081002761972	4.5278234752554446E-4
38-39	0.0	0.0	0.0	0.4223327346544516	4.905142098193398E-4
40-41	0.0	0.0	0.0	0.6192175921034758	4.905142098193398E-4
42-43	0.0	0.0	0.0	0.8004625926317219	5.282460721131352E-4
44-45	0.0	0.0	0.0	1.028193247505924	5.471120032600329E-4
46-47	0.0	0.0	0.0	1.3021077018277314	5.659779344069306E-4
48-49	0.0	0.0	0.0	1.5835307967460042	5.659779344069306E-4
50-51	0.0	0.0	0.0	1.8663122387068536	6.037097967007259E-4
52-53	0.0	0.0	0.0	2.15239521861841	6.414416589945214E-4
54-55	0.0	0.0	0.0	2.4772476870368414	6.414416589945214E-4
56-57	0.0	1.8865931146897685E-5	0.0	2.863565359131865	6.414416589945214E-4
58-59	0.0	3.773186229379537E-5	0.0	3.2689187557541093	6.414416589945214E-4
60-61	0.0	3.773186229379537E-5	0.0	3.6833089333957165	6.414416589945214E-4
62-63	0.0	3.773186229379537E-5	0.0	4.097227462758652	7.16905383582112E-4
64-65	0.0	3.773186229379537E-5	0.0	4.533011606320842	7.16905383582112E-4
66-67	0.0	3.773186229379537E-5	0.0	5.044014217365712	7.357713147290098E-4
68-69	0.0	3.773186229379537E-5	0.0	5.609916688048055	7.546372458759075E-4
70-71	0.0	3.773186229379537E-5	0.0	6.209834432588255	7.546372458759075E-4
72-73	0.0	3.773186229379537E-5	0.0	6.786698009266946	7.546372458759075E-4
74-75	0.0	3.773186229379537E-5	0.0	7.377277117889431	7.546372458759075E-4
76-77	0.0	3.773186229379537E-5	0.0	8.042980364338863	7.546372458759075E-4
78-79	0.0	3.773186229379537E-5	0.0	8.780393015077653	7.546372458759075E-4
80-81	0.0	3.773186229379537E-5	0.0	9.540105196432076	7.546372458759075E-4
82-83	0.0	3.773186229379537E-5	0.0	10.329191632582218	7.923691081697028E-4
84-85	0.0	3.773186229379537E-5	0.0	11.110543036962133	7.923691081697028E-4
86-87	0.0	3.773186229379537E-5	0.0	11.94788852498604	7.923691081697028E-4
88-89	0.0	3.773186229379537E-5	0.0	12.858396093997616	8.301009704634983E-4
90-91	0.0	3.773186229379537E-5	0.0	13.80484326184403	8.678328327572936E-4
92-93	0.0	3.773186229379537E-5	0.0	14.772005222089742	9.055646950510889E-4
94-95	0.0	3.773186229379537E-5	0.0	15.733941319407762	9.055646950510889E-4
96-97	0.0	3.773186229379537E-5	0.0	16.72366693330516	9.810284196386798E-4
98-99	0.0	3.773186229379537E-5	0.0	17.76897158036132	0.0010564921442262705
100-101	0.0	3.773186229379537E-5	0.0	18.90026714158504	0.0010564921442262705
102-103	0.0	3.773186229379537E-5	0.0	20.035430218693875	0.0010564921442262705
104-105	0.0	3.773186229379537E-5	0.0	21.16133012360958	0.0010564921442262705
106-107	0.0	3.773186229379537E-5	0.0	22.276759436738757	0.0010564921442262705
108-109	0.0	3.773186229379537E-5	0.0	23.435693587092686	0.0010942240065200657
110-111	0.0	3.773186229379537E-5	0.0	24.644396063812124	0.0011319558688138612
112-113	0.0	3.773186229379537E-5	0.0	25.88100125269783	0.0011319558688138612
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7765	0.0	36.655422	1
GTATCAA	11030	0.0	34.947678	1
ATCAACG	13550	0.0	27.994104	3
TCAACGC	13520	0.0	27.880043	4
TATCAAC	13630	0.0	27.874012	2
AACGCAG	13650	0.0	27.686085	6
CAACGCA	13645	0.0	27.62464	5
CATGGGG	5365	0.0	26.749382	4
TACATGG	11465	0.0	25.035002	2
GTACATG	11885	0.0	24.601307	1
ACGCAGA	15390	0.0	24.43526	7
ACATGGG	11425	0.0	24.392487	3
CGCAGAG	15500	0.0	24.18142	8
GAGTACT	9325	0.0	23.76883	12-13
AGTACTT	10190	0.0	21.692768	12-13
GCAGAGT	16945	0.0	21.664124	9
GTATAGG	610	0.0	21.520592	1
GTACTTT	10360	0.0	21.193218	14-15
AGAGTAC	15570	0.0	19.929108	10-11
ATGGGGA	3510	0.0	19.8493	5
>>END_MODULE