FastQCFastQC Report
Mon 15 Aug 2016
SRR2920903_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920903_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3869146
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98650.030.2034591
GTACATG147600.029.9168321
TACATGG148150.029.0674322
CATGGGG93300.028.3435674
ACATGGG153050.026.9303993
AACGCAG108950.026.5506736
TCAACGC109900.026.284354
GGTATCA76750.025.85531
CAACGCA111750.025.8492165
ATCAACG115250.025.1168633
TATCAAC118850.024.9073332
ACGCAGA121900.023.6299747
CGCAGAG123350.023.1586078
ATGGGGA49900.021.2457775
GCAGAGT143350.019.3035649
TTAGCGC3003.003528E-617.8679394
GTATATA20150.017.7443451
TATAGCG2351.6815071E-417.7412155
GTATAGG11600.017.4664521
GTATAAT18300.017.2587181
Produced by FastQC (version 0.11.5)