##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920903_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3869146
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.880135564799055	31.0	30.0	34.0	26.0	34.0
2	29.983790221408032	31.0	31.0	34.0	25.0	34.0
3	30.126800849593167	31.0	31.0	34.0	26.0	34.0
4	33.51854879603923	37.0	35.0	37.0	28.0	37.0
5	33.628141455504654	37.0	35.0	37.0	28.0	37.0
6	33.604849235464364	37.0	35.0	37.0	28.0	37.0
7	33.53866253690091	37.0	35.0	37.0	28.0	37.0
8	33.44131159692604	37.0	35.0	37.0	27.0	37.0
9	34.877420753830435	39.0	35.0	39.0	27.0	39.0
10-11	34.890433702941166	39.0	35.0	39.0	27.0	39.0
12-13	34.83285265017138	39.0	35.0	39.0	27.0	39.0
14-15	36.00994961161972	40.0	36.0	41.0	27.0	41.0
16-17	36.00785690175558	40.0	36.0	41.0	27.0	41.0
18-19	36.00426566999539	40.0	36.0	41.0	27.0	41.0
20-21	36.044865843780514	40.0	36.0	41.0	27.0	41.0
22-23	35.969936001381186	40.0	36.0	41.0	27.0	41.0
24-25	35.97588938230814	40.0	36.0	41.0	27.0	41.0
26-27	35.90884525939316	40.0	36.0	41.0	27.0	41.0
28-29	35.79454445502961	40.0	36.0	41.0	27.0	41.0
30-31	35.6420281116298	40.0	35.5	41.0	26.5	41.0
32-33	35.4981387365584	40.0	35.0	41.0	25.5	41.0
34-35	35.41234603191505	40.0	35.0	41.0	25.0	41.0
36-37	35.27069591067382	40.0	35.0	41.0	25.0	41.0
38-39	35.12674101726841	40.0	35.0	41.0	24.0	41.0
40-41	34.99362456211267	39.0	35.0	41.0	24.0	41.0
42-43	34.81230961561027	39.0	34.5	41.0	23.5	41.0
44-45	34.662025676984015	39.0	34.0	41.0	23.0	41.0
46-47	34.46045639011813	39.0	34.0	41.0	22.0	41.0
48-49	34.331693996556346	39.0	34.0	41.0	21.5	41.0
50-51	34.13323779976253	39.0	33.0	41.0	20.0	41.0
52-53	33.937058203541554	38.0	33.0	40.0	18.5	41.0
54-55	33.70328400634145	38.0	33.0	40.0	18.0	41.0
56-57	33.43757911952663	38.0	33.0	40.0	15.0	41.0
58-59	33.173714561301125	37.5	32.0	40.0	13.5	41.0
60-61	32.899614798717856	37.0	32.0	40.0	12.0	41.0
62-63	32.58374199371127	36.5	32.0	40.0	8.0	41.0
64-65	32.301030123960174	36.0	31.0	39.5	8.0	41.0
66-67	31.94623128721429	36.0	31.0	39.0	7.0	41.0
68-69	31.609638405994502	35.0	31.0	39.0	6.5	40.0
70-71	31.261134627641344	35.0	31.0	38.0	2.0	40.0
72-73	31.30806953265656	35.0	31.0	37.5	2.0	40.0
74-75	31.281922548283262	35.0	31.0	37.0	2.0	39.5
76-77	31.00781464436855	35.0	31.0	37.0	2.0	39.0
78-79	30.71183821959678	35.0	31.0	36.0	2.0	39.0
80-81	30.428994796267702	35.0	31.0	36.0	2.0	37.5
82-83	30.180479749278007	35.0	31.0	35.5	2.0	37.0
84-85	29.940463089270864	35.0	31.0	35.0	2.0	37.0
86-87	29.73608581842091	35.0	31.0	35.0	2.0	36.0
88-89	29.54039676972645	35.0	30.5	35.0	2.0	36.0
90-91	29.363317124760865	35.0	30.0	35.0	2.0	36.0
92-93	29.19506242462807	35.0	30.0	35.0	2.0	35.5
94-95	29.07670620338442	34.0	30.0	35.0	2.0	35.0
96-97	28.97292606688918	34.0	30.0	35.0	2.0	35.0
98-99	28.836783233302647	34.0	30.0	35.0	2.0	35.0
100-101	28.709270469504123	34.0	29.5	35.0	2.0	35.0
102-103	27.36079021055292	32.5	26.0	34.5	2.0	35.0
104-105	28.356563954939926	34.0	29.0	35.0	2.0	35.0
106-107	28.495213672474495	34.0	29.0	35.0	2.0	35.0
108-109	28.41826245378179	34.0	29.0	35.0	2.0	35.0
110-111	28.30843460029681	34.0	29.0	35.0	2.0	35.0
112-113	28.181133640343376	34.0	29.0	35.0	2.0	35.0
114-115	28.05549015209041	34.0	29.0	35.0	2.0	35.0
116-117	27.833061611011836	34.0	29.0	35.0	2.0	35.0
118-119	27.624802475791817	34.0	29.0	35.0	2.0	35.0
120-121	27.42941220103868	34.0	27.5	35.0	2.0	35.0
122-123	27.129300884484586	34.0	27.0	35.0	2.0	35.0
124-125	25.84284270999337	33.0	23.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	130415.0
3	19106.0
4	13082.0
5	13978.0
6	19409.0
7	19957.0
8	17921.0
9	19149.0
10	20767.0
11	22169.0
12	22962.0
13	22817.0
14	23420.0
15	21679.0
16	17511.0
17	15986.0
18	15304.0
19	15100.0
20	15351.0
21	16679.0
22	19912.0
23	25029.0
24	34415.0
25	43898.0
26	49147.0
27	53368.0
28	62592.0
29	73263.0
30	87273.0
31	105660.0
32	132800.0
33	170969.0
34	229215.0
35	335490.0
36	605198.0
37	987421.0
38	370091.0
39	643.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10713610749959	18.569355968641123	12.209816192974852	25.113691730884437
2	16.134017056142078	20.787014616037496	38.66596399661538	24.413004331205045
3	19.000040648333147	24.77259587763764	29.102420077480385	27.12494339654883
4	12.772487136766857	16.758141710264525	36.35231605492489	34.11705509804373
5	13.645916989901409	37.99795884446364	34.36279131903074	13.99333284660421
6	32.32722654039619	36.75056553402894	17.051662759309284	13.870545166265583
7	27.893533851083387	32.723792008544535	21.381303869050083	18.001370271322
8	25.456010679443363	36.046795002806974	20.08133762097807	18.41585669677159
9	25.8820446043467	16.48546145429699	19.446545130718455	38.18594881063786
10-11	24.666600011459867	26.73769283392286	27.823741558534117	20.77196559608316
12-13	25.150820418847964	24.4012028128055	27.604026223295307	22.843950545051225
14-15	22.697786315212667	25.8303920697559	24.903025922306153	26.568795692725285
16-17	22.80763310028839	28.385101867226332	25.79601775468035	23.01124727780493
18-19	22.89277351082655	27.62418578280791	27.242960069328813	22.24008063703673
20-21	23.73989562274802	26.96460621362382	26.841185680269692	22.454312483358464
22-23	23.396407833551386	26.7592246100978	26.536715214092883	23.30765234225793
24-25	23.06097510512949	26.951826641080416	26.769421154174406	23.217777099615688
26-27	23.01010812424919	27.07477394418592	26.772465232105606	23.142652699459283
28-29	22.97371987523342	27.206291608257764	26.831578048944504	22.98841046756431
30-31	23.303358555355274	27.028819262550375	26.676979104124023	22.99084307797033
32-33	22.909373452525937	27.23987595506106	26.584823596524682	23.265926995888318
34-35	23.152900413821072	27.14245420894401	26.70010445428938	23.00454092294553
36-37	23.263657448540386	27.028736582063928	26.594639628140076	23.112966341255607
38-39	23.048967970941174	27.22811406272146	26.580697006282904	23.14222096005446
40-41	23.132404097370355	27.129960345942916	26.732462759819946	23.005172796866784
42-43	23.03576255206993	27.14211259390693	26.72283731162034	23.099287542402802
44-45	22.999828007826288	27.26871255452346	26.575222005687383	23.15623743196287
46-47	22.981965402922846	27.120173705667895	26.66505109671035	23.232809794698905
48-49	22.863244851703005	27.201529499645126	26.577306206765112	23.35791944188676
50-51	22.813548183787113	27.311061993191277	26.55438798125048	23.321001841771132
52-53	22.916592850032444	27.145038112659748	26.453390258997462	23.48497877831035
54-55	22.84684520184814	27.143359075501035	26.430980502050506	23.578815220600323
56-57	22.920904876119383	27.1394295957946	26.429292020783873	23.510373507302145
58-59	22.80939629077452	26.90516428768156	26.5104368031302	23.775002618413723
60-61	22.946399579047718	26.859398808448475	26.581910664542598	23.61229094796121
62-63	22.683027250196616	26.822694885056862	26.645590719203778	23.848687145542744
64-65	22.684549144509788	26.72721769107444	26.65493713078243	23.933296033633347
66-67	22.7182815876802	26.888778515863265	26.5323880103515	23.86055188610504
68-69	22.833491419079817	26.607958340364757	26.60745401773793	23.951096222817494
70-71	22.601816066119362	26.50950167769462	26.848350862846704	24.040331393339315
72-73	22.68453467002866	26.44673792610946	26.855535610175412	24.01319179368647
74-75	22.56562073899177	26.49241075844573	26.87945981487384	24.062508687688663
76-77	22.58162505237349	26.314290074124653	27.043988558009136	24.060096315492725
78-79	22.6006112683793	26.348742110225892	27.02816913792952	24.022477483465288
80-81	22.592713662122737	26.369508305508653	26.99848271335437	24.039295319014236
82-83	22.55250167395517	26.25041024509704	27.02323571331956	24.17385236762823
84-85	22.569546190112018	26.228433187267864	26.98610201237233	24.21591861024779
86-87	22.534211600580896	26.09713688170301	27.112529402690427	24.256122115025665
88-89	22.599473701020234	26.01277511832417	27.07673902555683	24.311012155098766
90-91	22.589413592076333	26.095095707082073	27.07431723327473	24.241173467566867
92-93	22.56081248560719	26.02971050687642	27.058415699097104	24.351061308419286
94-95	22.421787814248564	25.91036229831905	27.197228030564595	24.470621856867787
96-97	22.46870021679151	25.833629745651727	27.168448437638794	24.529221599917975
98-99	22.315088335654412	25.949882554004017	27.120709297013924	24.614319813327647
100-101	22.24583689104883	26.070210815006895	27.165297624988945	24.518654668955328
102-103	22.161290535284188	26.096424513095627	27.183278305189184	24.559006646430998
104-105	21.95889670651328	26.16146023225326	27.118632234642504	24.761010826590958
106-107	21.873650401226826	26.319367760655204	27.09148695721885	24.71549488089912
108-109	21.85933615050148	26.365598360747946	27.131201583289705	24.643863905460865
110-111	21.848784917828347	26.5212498268842	27.050845160703833	24.57912009458362
112-113	21.839184800642006	26.59418248984841	27.07876379396359	24.48786891554599
114-115	21.691891696172018	26.677111001337117	27.024968123873343	24.60602917861752
116-117	21.634268024849526	26.605337302236464	27.022819598067088	24.73757507484692
118-119	21.669487145302774	26.49886122663213	27.07043972440818	24.761211903656914
120-121	21.664326764408504	26.61361767510053	27.05517966686361	24.666875893627356
122-123	21.67765171122171	26.681509655593604	27.020416523510445	24.62042210967424
124-125	21.64571613320401	26.714645974781764	26.842470093760102	24.797167798254122
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3330.0
1	2345.5
2	1142.0
3	837.5
4	692.0
5	545.0
6	479.0
7	525.5
8	575.0
9	623.0
10	659.5
11	718.0
12	783.5
13	906.5
14	1105.5
15	1288.5
16	1465.0
17	1700.0
18	2095.0
19	2664.5
20	3292.0
21	4118.0
22	5288.5
23	6960.0
24	8869.0
25	11387.0
26	15283.5
27	20150.5
28	24417.0
29	29852.0
30	36778.0
31	43222.0
32	49951.0
33	56781.5
34	63600.5
35	70252.0
36	78343.0
37	86779.5
38	93710.0
39	100416.5
40	107187.0
41	113511.0
42	119143.5
43	127385.0
44	135484.5
45	140815.0
46	146395.0
47	151344.5
48	157209.0
49	162549.5
50	166375.5
51	165544.0
52	157889.0
53	151382.5
54	145670.5
55	135982.0
56	123439.0
57	109924.5
58	98103.5
59	86753.5
60	73429.0
61	60642.5
62	49414.5
63	37680.5
64	27617.5
65	20580.0
66	15126.5
67	11619.5
68	9122.5
69	6984.0
70	5377.5
71	4158.5
72	3138.5
73	2486.0
74	1973.5
75	1529.0
76	1094.0
77	736.5
78	524.0
79	379.5
80	264.5
81	197.5
82	149.0
83	107.5
84	84.0
85	73.0
86	55.0
87	38.0
88	31.5
89	24.0
90	16.0
91	16.0
92	15.5
93	10.5
94	10.5
95	14.5
96	11.5
97	8.5
98	10.5
99	14.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16820249222955144
2	0.1495678891414281
3	0.17443125692336242
4	0.14512246371679952
5	0.17130395182812952
6	0.1089387684000552
7	0.10901630489001965
8	0.09759259536859038
9	0.1035629050958532
10-11	0.08998109660374666
12-13	0.07860907807562702
14-15	0.07845400509569812
16-17	0.0778078676793277
18-19	0.07579191894025193
20-21	0.06953730874978613
22-23	0.06055599866223709
24-25	0.06425190468387598
26-27	0.07493901755064296
28-29	0.07045482388103215
30-31	0.06765058749398446
32-33	0.07667066582651573
34-35	0.0739568886777599
36-37	0.0764768246016046
38-39	0.07190217169370192
40-41	0.0764768246016046
42-43	0.07627006062836605
44-45	0.06955023149811353
46-47	0.06734044153412666
48-49	0.058423745188214665
50-51	0.0642906729288582
52-53	0.06675891785939327
54-55	0.06881363484345125
56-57	0.07191509444202933
58-59	0.059470487802734756
60-61	0.06912378080330904
62-63	0.06448451415376934
64-65	0.05865635465810802
66-67	0.06359284451917814
68-69	0.0665133856411725
70-71	0.06155105028344757
72-73	0.05676963340230635
74-75	0.06005201147746815
76-77	0.06942100401483944
78-79	0.06285624786451584
80-81	0.06138305455519125
82-83	0.06603524395305838
84-85	0.06758597375234741
86-87	0.061137522336970486
88-89	0.060284620947361506
90-91	0.06337315779761218
92-93	0.056795478898961166
94-95	0.06670722686608363
96-97	0.05340971883718009
98-99	0.0529703453940482
100-101	0.050760555430061305
102-103	0.0486541474526937
104-105	0.04693542192514834
106-107	0.05830744045326799
108-109	0.058423745188214665
110-111	0.06498850133853827
112-113	0.06484635110693678
114-115	0.06761181924900224
116-117	0.07119142053569444
118-119	0.06423898193554857
120-121	0.06542787478167017
122-123	0.06330854405597515
124-125	0.0748485583123511
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3869146.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.77687904981006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.74708319278824	31.01250033293687
2	13.85718858091318	12.686776912777434
3	6.485172393894613	8.906128568774427
4	3.556374411369622	6.511988851404266
5	2.1819434272145766	4.994128018056485
6	1.425037596392298	3.9140264214889355
7	0.9761823482920163	3.1280606901826236
8	0.6871628915085309	2.516493805768302
9	0.5159517016118713	2.1256792776207294
>10	2.4476735673926346	18.685169172377588
>50	0.08981843662207233	2.7818544540280756
>100	0.02956206013666427	2.1760670638948163
>500	3.96382884603263E-4	0.12133935115239892
>1k	4.5300897901677826E-4	0.43978707953700147
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.5845496654817367E-5	0.0	0.0	0.0	0.0
6	2.5845496654817367E-5	0.0	0.0	0.0	0.0
7	2.5845496654817367E-5	0.0	0.0	0.0	0.0
8	2.5845496654817367E-5	0.0	0.0	2.5845496654817367E-5	0.0
9	2.5845496654817367E-5	0.0	0.0	5.1690993309634735E-5	0.0
10-11	5.1690993309634735E-5	0.0	0.0	5.1690993309634735E-5	0.0
12-13	5.1690993309634735E-5	0.0	0.0	5.1690993309634735E-5	0.0
14-15	5.1690993309634735E-5	0.0	0.0	5.1690993309634735E-5	0.0
16-17	5.1690993309634735E-5	0.0	0.0	7.75364899644521E-5	2.5845496654817367E-5
18-19	5.1690993309634735E-5	0.0	0.0	1.0338198661926947E-4	2.5845496654817367E-5
20-21	7.75364899644521E-5	0.0	0.0	1.9384122491113024E-4	2.5845496654817367E-5
22-23	7.75364899644521E-5	0.0	0.0	4.781416881141213E-4	6.461374163704342E-5
24-25	9.045923829186078E-5	0.0	0.0	8.916696345911991E-4	7.75364899644521E-5
26-27	1.0338198661926947E-4	0.0	0.0	0.0017316482758727636	1.0338198661926947E-4
28-29	1.2922748327408683E-4	0.0	0.0	0.0051820220792908826	1.0338198661926947E-4
30-31	2.8430046320299107E-4	0.0	0.0	0.01885428980968927	1.1630473494667816E-4
32-33	2.8430046320299107E-4	0.0	0.0	0.0553481310862914	1.2922748327408683E-4
34-35	2.9722321153039975E-4	0.0	0.0	0.11170423654212067	1.550729799289042E-4
36-37	3.101459598578084E-4	0.0	0.0	0.2045024922812424	1.550729799289042E-4
38-39	3.3599145651262577E-4	0.0	0.0	0.3485911361318492	1.550729799289042E-4
40-41	3.3599145651262577E-4	0.0	0.0	0.5091950523448844	1.550729799289042E-4
42-43	3.3599145651262577E-4	0.0	0.0	0.6632342124075958	1.6799572825631289E-4
44-45	3.618369531674431E-4	0.0	0.0	0.8494122475605728	1.809184765837216E-4
46-47	3.8768244982226053E-4	0.0	0.0	1.0735443945511491	2.45532218220765E-4
48-49	4.006051981496692E-4	0.0	0.0	1.3053784995448607	2.8430046320299107E-4
50-51	4.2645069480448655E-4	0.0	0.0	1.5451859402565837	2.8430046320299107E-4
52-53	4.3937344313189523E-4	0.0	0.0	1.7823054493162056	3.101459598578084E-4
54-55	4.5229619145930396E-4	0.0	0.0	2.0472734810213935	3.3599145651262577E-4
56-57	4.781416881141213E-4	0.0	0.0	2.3609085829276024	3.618369531674431E-4
58-59	5.039871847689387E-4	0.0	0.0	2.6958791423223625	3.8768244982226053E-4
60-61	5.169099330963473E-4	0.0	0.0	3.0508670388762793	3.8768244982226053E-4
62-63	5.427554297511647E-4	0.0	0.0	3.4000655441795167	3.8768244982226053E-4
64-65	5.427554297511647E-4	0.0	0.0	3.773584661834937	3.8768244982226053E-4
66-67	5.427554297511647E-4	0.0	0.0	4.210089254838147	4.264506948044866E-4
68-69	5.427554297511647E-4	0.0	0.0	4.687390964310987	4.6521893978671263E-4
70-71	5.556781780785734E-4	0.0	0.0	5.206859601576162	4.9106443644153E-4
72-73	5.944464230607995E-4	0.0	0.0	5.719143190771296	5.169099330963473E-4
74-75	5.944464230607995E-4	0.0	0.0	6.239387192936116	5.169099330963473E-4
76-77	5.944464230607995E-4	0.0	0.0	6.808039810335408	5.169099330963473E-4
78-79	5.944464230607995E-4	0.0	0.0	7.440685877452027	5.169099330963473E-4
80-81	5.944464230607995E-4	0.0	0.0	8.10837843803258	5.169099330963473E-4
82-83	5.944464230607995E-4	0.0	0.0	8.788761137470646	5.169099330963473E-4
84-85	6.978284096800689E-4	0.0	0.0	9.47084963968793	5.169099330963473E-4
86-87	8.012103962993384E-4	0.0	0.0	10.195621979630648	5.169099330963473E-4
88-89	8.012103962993384E-4	0.0	0.0	10.981195850453823	5.169099330963473E-4
90-91	8.012103962993384E-4	0.0	0.0	11.80829051165296	5.169099330963473E-4
92-93	8.012103962993384E-4	0.0	0.0	12.6590079567946	5.427554297511647E-4
94-95	8.012103962993384E-4	0.0	0.0	13.506029495914602	5.556781780785734E-4
96-97	8.012103962993384E-4	0.0	0.0	14.372150857062515	5.686009264059821E-4
98-99	8.658241379363818E-4	0.0	0.0	15.289653065560204	5.686009264059821E-4
100-101	8.787468862637905E-4	1.2922748327408684E-5	0.0	16.27670550555601	5.686009264059821E-4
102-103	9.045923829186079E-4	2.5845496654817367E-5	0.0	17.284576493107267	5.944464230607995E-4
104-105	9.045923829186079E-4	2.5845496654817367E-5	0.0	18.28174744504343	5.944464230607995E-4
106-107	9.045923829186079E-4	5.1690993309634735E-5	0.0	19.27938361591938	6.073691713882082E-4
108-109	9.045923829186079E-4	5.1690993309634735E-5	0.0	20.308228224006022	6.202919197156168E-4
110-111	9.045923829186079E-4	5.1690993309634735E-5	0.0	21.385584829313757	6.461374163704342E-4
112-113	9.045923829186079E-4	5.1690993309634735E-5	0.0	22.496902417225918	6.719829130252515E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9865	0.0	30.203459	1
GTACATG	14760	0.0	29.916832	1
TACATGG	14815	0.0	29.067432	2
CATGGGG	9330	0.0	28.343567	4
ACATGGG	15305	0.0	26.930399	3
AACGCAG	10895	0.0	26.550673	6
TCAACGC	10990	0.0	26.28435	4
GGTATCA	7675	0.0	25.8553	1
CAACGCA	11175	0.0	25.849216	5
ATCAACG	11525	0.0	25.116863	3
TATCAAC	11885	0.0	24.907333	2
ACGCAGA	12190	0.0	23.629974	7
CGCAGAG	12335	0.0	23.158607	8
ATGGGGA	4990	0.0	21.245777	5
GCAGAGT	14335	0.0	19.303564	9
TTAGCGC	300	3.003528E-6	17.867939	4
GTATATA	2015	0.0	17.744345	1
TATAGCG	235	1.6815071E-4	17.741215	5
GTATAGG	1160	0.0	17.466452	1
GTATAAT	1830	0.0	17.258718	1
>>END_MODULE