FastQCFastQC Report
Mon 15 Aug 2016
SRR2920903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3869146
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92050.23790779670759388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79630.2058076898623107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52380.13537871147793337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88100.047.406941
GTATCAA129850.040.4352841
TCAACGC160750.031.8567334
ATCAACG163000.031.5631183
TATCAAC166850.031.0148582
CAACGCA165600.030.9596885
AACGCAG169300.030.408036
GTACATG135050.027.5682891
TACATGG134400.026.9389442
ACGCAGA191100.026.6838327
CGCAGAG192350.026.353038
CATGGGG83400.025.8461764
ACATGGG139550.024.8766843
GAGTACT131150.023.43372212-13
GCAGAGT212450.023.1057289
GTACTTT140250.021.89203514-15
AGTACTT134950.021.62745512-13
AGAGTAC192000.021.38391110-11
CAGAGTA206250.021.305710-11
ACTTTTT175300.018.05692316-17
Produced by FastQC (version 0.11.5)