FastQCFastQC Report
Mon 15 Aug 2016
SRR2920902_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920902_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3680812
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76400.029.7608241
GTACATG100500.028.6236691
TACATGG103200.027.0615272
ACATGGG106700.025.8719443
ATCAACG87250.025.5953163
TCAACGC87350.025.5660154
CAACGCA87650.025.4261955
AACGCAG92350.024.1269256
TATCAAC92900.024.0890032
GAGTACA75750.021.6918681
CATGGGG75100.020.9435944
AGTACAT72200.020.7694632
ACGCAGA107600.020.3654777
GGTATCA55100.020.2150631
GTGTAGC25750.020.0194511
CGCAGAG109150.019.6554688
AGAGTAC85250.016.80416310-11
GCAGAGT122000.016.720979
TAGCCCT28550.016.7041444
GTATAGG9650.016.2167171
Produced by FastQC (version 0.11.5)