##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920902_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3680812
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.247382371063775	31.0	28.0	31.0	16.0	34.0
2	28.250871003463367	31.0	28.0	33.0	16.0	34.0
3	28.33468185824215	31.0	28.0	34.0	16.0	34.0
4	31.76665040213953	35.0	32.0	37.0	19.0	37.0
5	31.719517595574022	35.0	32.0	37.0	19.0	37.0
6	31.848841777303488	35.0	32.0	37.0	19.0	37.0
7	31.67602230160084	35.0	32.0	37.0	18.0	37.0
8	31.617378176337176	35.0	32.0	37.0	18.0	37.0
9	32.84384885726302	37.0	32.0	39.0	17.0	39.0
10-11	32.637414380305216	37.0	32.0	39.0	17.0	39.0
12-13	32.45417668710056	37.0	32.0	39.0	17.0	39.0
14-15	33.16274710580166	38.0	32.0	40.0	16.0	41.0
16-17	33.04367161376348	38.0	32.0	40.0	13.5	41.0
18-19	32.92377415635463	38.0	32.0	40.0	11.0	41.0
20-21	32.8243181124165	38.0	32.0	40.0	10.0	41.0
22-23	32.59359171291552	38.0	31.0	40.0	10.0	41.0
24-25	32.39172076704814	37.0	31.0	40.0	10.0	41.0
26-27	32.424726527733554	38.0	31.0	40.0	9.5	41.0
28-29	32.25104773077245	37.0	31.0	40.0	8.0	41.0
30-31	32.00321002539657	37.0	30.0	40.0	8.0	41.0
32-33	31.74640093001218	37.0	30.0	40.0	2.0	41.0
34-35	31.414137288185323	37.0	30.0	40.0	2.0	41.0
36-37	31.09780002347308	36.5	30.0	40.0	2.0	41.0
38-39	30.89228490887337	36.0	29.0	40.0	2.0	41.0
40-41	30.67989929939372	36.0	29.0	39.5	2.0	41.0
42-43	30.439752424193358	36.0	28.0	39.0	2.0	40.5
44-45	30.15643939435103	35.5	27.5	39.0	2.0	40.0
46-47	29.90315995492299	35.0	27.0	39.0	2.0	40.0
48-49	29.591384455386475	35.0	26.5	39.0	2.0	40.0
50-51	28.493263307118106	34.0	25.0	38.0	2.0	39.5
52-53	28.957042359131627	34.5	26.5	38.5	2.0	39.5
54-55	29.59237459017195	35.0	27.0	39.0	2.0	40.0
56-57	29.540397879598306	35.0	27.0	39.0	2.0	40.0
58-59	29.305104281337922	35.0	26.5	39.0	2.0	40.0
60-61	28.974395323640543	35.0	26.0	39.0	2.0	40.0
62-63	28.574907792085007	34.0	26.0	38.0	2.0	40.0
64-65	28.154986589915485	34.0	25.0	38.0	2.0	40.0
66-67	27.76405083986903	34.0	23.5	37.5	2.0	40.0
68-69	27.313523755084475	33.5	22.5	37.0	2.0	39.5
70-71	26.977543677862386	33.0	22.0	36.5	2.0	39.0
72-73	26.50465617369211	33.0	21.0	36.0	2.0	39.0
74-75	26.047958982963543	32.5	20.0	35.5	2.0	38.5
76-77	25.615531708764262	32.0	18.0	35.0	2.0	37.5
78-79	25.13657394075003	32.0	15.0	35.0	2.0	37.0
80-81	24.66190299857749	31.5	9.0	35.0	2.0	36.0
82-83	24.15923959169879	31.0	4.5	34.5	2.0	36.0
84-85	23.726320034818407	31.0	2.0	34.0	2.0	35.5
86-87	23.247207817188162	31.0	2.0	34.0	2.0	35.0
88-89	22.689717241739054	30.0	2.0	34.0	2.0	35.0
90-91	22.159436423267472	30.0	2.0	34.0	2.0	35.0
92-93	21.584671534433163	29.5	2.0	34.0	2.0	35.0
94-95	20.913093089242267	29.0	2.0	34.0	2.0	35.0
96-97	20.243912348688276	29.0	2.0	34.0	2.0	35.0
98	19.83074875869781	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	213794.0
3	36080.0
4	26542.0
5	21278.0
6	19676.0
7	20120.0
8	22694.0
9	26026.0
10	28618.0
11	30493.0
12	31086.0
13	30741.0
14	29420.0
15	31182.0
16	33521.0
17	35645.0
18	36829.0
19	36962.0
20	38196.0
21	40400.0
22	43815.0
23	48548.0
24	54890.0
25	62849.0
26	72494.0
27	83055.0
28	94699.0
29	109714.0
30	126289.0
31	146679.0
32	173649.0
33	208495.0
34	255994.0
35	317757.0
36	394423.0
37	424955.0
38	248847.0
39	24351.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24417548243651	17.419890296884635	11.858038899303166	25.477895321375687
2	16.383584946934914	19.53963610572527	38.68458918208709	25.39218976525273
3	20.640814144931255	22.715984838583193	28.3016283931197	28.341572623365852
4	13.346247476022382	14.862060598287648	36.28136817452733	35.510323751162645
5	14.464714240799312	36.518188060823334	34.29659325934539	14.72050443903196
6	34.2595815714481	34.37844004101079	16.970212284922283	14.391766102618828
7	29.760506051202906	30.91950025269954	20.40738969529327	18.912604000804293
8	26.890665658097905	34.05438391854503	19.84731268864814	19.20763773470892
9	26.778683616549827	15.730822438092465	19.168406319040475	38.322087626317234
10-11	25.952999766491708	25.00149763170431	27.43088039145716	21.61462221034682
12-13	26.584828794027516	22.281887746611602	27.225592058708735	23.907691400652148
14-15	23.415613614995987	24.12090228154139	24.82962780227574	27.63385630118688
16-17	23.728398878877226	26.967235741168004	25.584742253700217	23.719623126254554
18-19	23.600662644290985	26.3458314518506	26.949690589796088	23.103815314062327
20-21	24.267739538917787	25.67163610979132	26.7067430401627	23.353881311128195
22-23	23.726614297250396	25.58245939552769	26.59679787211911	24.094128435102803
24-25	23.630011023470068	25.717184787883152	26.783185606148134	23.869618582498646
26-27	23.527984414972163	25.916293958463577	26.7044764814225	23.851245145141757
28-29	23.457435971457304	26.266377928815597	26.59900273719242	23.677183362534674
30-31	23.867224078903913	25.972945754239372	26.44164500034657	23.718185166510146
32-33	23.50907898744895	25.959874361802466	26.46748409210533	24.063562558643252
34-35	23.905192793618525	25.966680702263282	26.499333638581433	23.628792865536756
36-37	23.863120371441383	25.80282519522755	26.546849626956732	23.78720480637434
38-39	23.721283669880854	25.96910917167186	26.50387152272068	23.805735635726606
40-41	23.85593795880256	25.805427785714336	26.6321614152295	23.70647284025361
42-43	23.803931745397627	25.72874984445914	26.443282708877724	24.024035701265504
44-45	23.71280690040396	25.843041317444087	26.444037202818375	24.00011457933357
46-47	23.665040813200445	25.818865856381564	26.472594532314673	24.043498798103318
48-49	23.672088594802183	25.84782922385714	26.432874827572757	24.04720735376792
50-51	23.477704309989996	25.986257544344117	26.58713197498089	23.948906170684996
52-53	23.58168145776268	25.908126714695424	26.58009899790279	23.930092829639108
54-55	23.365752218113872	25.73325971235962	26.83326186858438	24.067726200942133
56-57	23.348901577898022	25.771656772459238	26.665805351739824	24.213636297902912
58-59	23.357900806264713	25.597755384794397	26.762253874557068	24.282089934383826
60-61	23.256597925809753	25.574785678512924	27.037092668437744	24.131523727239575
62-63	23.168392432863165	25.511922593067315	27.001910839406694	24.317774134662823
64-65	23.15522274365664	25.494513202553048	26.86102346618653	24.48924058760378
66-67	23.06193156915339	25.550357013234283	26.78784728615753	24.599864131454797
68-69	23.3096287848611	25.338257040428235	26.77230264701549	24.57981152769517
70-71	23.12917795696813	25.185021860641683	26.837755640787332	24.84804454160285
72-73	23.23750592891249	25.14795525288354	26.877241278891816	24.73729753931215
74-75	23.084661397213075	25.347820195912877	26.803303872702156	24.764214534171888
76-77	23.146845016018265	25.062194047609477	26.916831383829997	24.874129552542264
78-79	23.213979996641392	25.111567467716995	26.853464820739635	24.820987714901978
80-81	23.308452519344307	25.0676643531795	26.80671287097127	24.81717025650492
82-83	23.36877624154126	25.047294245663426	26.83191331312737	24.752016199667942
84-85	23.270576918777465	24.98871235201321	26.87856181186926	24.86214891734006
86-87	23.23941665414947	24.888578783503036	26.922457266838716	24.949547295508776
88-89	23.271183315823983	24.75188266443245	26.867173292401812	25.10976072734175
90-91	23.328199444820196	24.672181200042917	26.91261432808888	25.087005027048008
92-93	23.574957014956055	24.505107971423552	26.90265988575582	25.017275127864576
94-95	23.4628962065546	24.37528680488924	27.02707492686561	25.134742061690556
96-97	23.584484323126386	24.201405399981677	27.014004920972152	25.20010535591978
98	23.667871768445963	24.119757670936327	26.93909601007705	25.27327455054066
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	94.0
1	80.5
2	108.5
3	169.5
4	165.5
5	182.5
6	333.5
7	595.5
8	891.0
9	1064.5
10	1101.0
11	1178.5
12	1347.0
13	1511.0
14	1690.5
15	1885.0
16	2064.5
17	2104.0
18	2140.5
19	2227.0
20	2261.0
21	2433.0
22	2656.5
23	3041.0
24	3691.0
25	4747.0
26	6333.5
27	8261.0
28	10857.5
29	14704.5
30	20102.0
31	27570.5
32	35469.0
33	43137.5
34	52143.0
35	62009.5
36	71996.0
37	81225.5
38	90372.0
39	99341.0
40	107400.0
41	115068.5
42	123951.0
43	133985.0
44	142196.5
45	149918.5
46	158356.0
47	166405.0
48	171271.0
49	171159.5
50	170120.0
51	168544.0
52	164090.0
53	159417.0
54	152479.5
55	143022.5
56	131500.0
57	118676.0
58	107341.5
59	92593.5
60	76556.5
61	62207.5
62	49511.0
63	37820.5
64	27607.0
65	20604.0
66	15119.0
67	11352.0
68	8680.5
69	6617.0
70	5153.5
71	4167.0
72	3672.5
73	3259.5
74	2635.5
75	1939.5
76	1295.0
77	782.0
78	470.0
79	275.0
80	169.5
81	96.5
82	59.5
83	41.0
84	22.0
85	13.0
86	13.0
87	14.0
88	8.5
89	6.0
90	6.0
91	5.0
92	5.5
93	4.5
94	2.0
95	2.5
96	2.5
97	1.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3904301550853453
2	0.024369622789754002
3	0.018501352418977116
4	0.016545262295384822
5	0.022902555197059776
6	0.02187017429849718
7	0.014996690947540922
8	0.009481603515745982
9	0.0
10-11	1.3583959191613154E-5
12-13	0.0054743355542201015
14-15	0.00327373416517877
16-17	0.005868270370776882
18-19	0.035630724959601304
20-21	0.07700746465725497
22-23	0.09613367919904629
24-25	0.062404708526270836
26-27	0.07244325436887296
28-29	0.055490473297739734
30-31	0.051809220356812576
32-33	0.019588069154306168
34-35	0.34659471877400966
36-37	0.5299781678607872
38-39	0.48312709260891346
40-41	0.49131822000145614
42-43	0.43957691944060173
44-45	0.41375381301734504
46-47	0.4115260437099206
48-49	0.4013516582754023
50-51	0.3981458439061816
52-53	0.45902914900299174
54-55	0.46188178043323047
56-57	0.43123636849695124
58-59	0.4164434369372845
60-61	0.42967421318991567
62-63	0.41147170787315407
64-65	0.45860804626805174
66-67	0.481211754362896
68-69	0.4225154666959356
70-71	0.37893812560924056
72-73	0.3641180261311906
74-75	0.34647246314128516
76-77	0.3438235910989206
78-79	0.3430764733433818
80-81	0.36026018172077245
82-83	0.3554242922485582
84-85	0.3555193799628995
86-87	0.34027817774990954
88-89	0.38578444104181364
90-91	0.3627324622936461
92-93	0.35227281371610397
94-95	0.3599477506593654
96-97	0.35981191106744925
98	0.3551390291055343
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3680812.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.21428913709857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.14162325820854	36.225403291073185
2	12.157196532516714	12.209299635606527
3	5.544515446586031	8.352417052799405
4	3.044728649517061	6.115555390034294
5	1.8928112753626496	4.752308633151019
6	1.2428795140774007	3.7446186769475505
7	0.8604547749027582	3.024498739945491
8	0.6133441689032086	2.4638913150287314
9	0.459968556117112	2.078729466375457
>10	1.9528365974645037	15.915366826216983
>50	0.06256534844180206	2.147812652510286
>100	0.02576492899429792	2.3563859402764873
>500	9.832116863105374E-4	0.35840803235010915
>1k	3.2773722146767724E-4	0.25530434768438903
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.716791838322631E-5
2	0.0	0.0	0.0	0.0	2.716791838322631E-5
3	0.0	0.0	0.0	0.0	2.716791838322631E-5
4	0.0	0.0	0.0	0.0	2.716791838322631E-5
5	0.0	0.0	0.0	0.0	2.716791838322631E-5
6	0.0	0.0	0.0	0.0	5.433583676645262E-5
7	0.0	0.0	0.0	0.0	8.150375514967894E-5
8	0.0	0.0	0.0	0.0	8.150375514967894E-5
9	0.0	0.0	0.0	2.716791838322631E-5	1.6300751029935787E-4
10-11	0.0	0.0	0.0	4.075187757483946E-5	1.6300751029935787E-4
12-13	0.0	0.0	0.0	5.433583676645262E-5	1.6300751029935787E-4
14-15	0.0	0.0	0.0	8.150375514967894E-5	2.4451126544903677E-4
16-17	0.0	0.0	0.0	9.508771434129208E-5	4.6185461251484726E-4
18-19	0.0	0.0	0.0	1.494235511077447E-4	4.6185461251484726E-4
20-21	0.0	0.0	0.0	3.260150205987157E-4	4.890225308980735E-4
22-23	0.0	0.0	0.0	5.841102452393656E-4	6.927819187722709E-4
24-25	0.0	0.0	0.0	0.0011274686129038917	9.237092250296945E-4
26-27	0.0	0.0	0.0	0.001657243021376805	9.916290209877603E-4
28-29	0.0	0.0	0.0	0.008109623637393053	0.0010052129801793735
30-31	0.0	0.0	0.0	0.03371538671358385	0.0010187969393709866
32-33	0.0	0.0	0.0	0.08491332890677383	0.0010867167353290524
34-35	0.0	0.0	0.0	0.15339006719169573	0.0011682204904787314
36-37	0.0	1.3583959191613154E-5	0.0	0.26515888342028876	0.0011682204904787314
38-39	0.0	2.716791838322631E-5	0.0	0.44300007715688816	0.0011682204904787314
40-41	0.0	2.716791838322631E-5	0.0	0.6463791141737203	0.0011953884088619577
42-43	0.0	2.716791838322631E-5	0.0	0.8618071229935134	0.0012361402864367972
44-45	0.0	2.716791838322631E-5	0.0	1.1250370842085933	0.0012904761232032497
46-47	0.0	2.716791838322631E-5	0.0	1.4362999251252169	0.0013312280007780892
48-49	0.0	2.716791838322631E-5	0.0	1.765466424256387	0.0014398996743109943
50-51	0.0	2.716791838322631E-5	0.0	2.131771467817427	0.0014398996743109943
52-53	0.0	2.716791838322631E-5	0.0	2.5061589670974773	0.0015214034294606734
54-55	0.0	2.716791838322631E-5	0.0	2.920673481829553	0.0016029071846103524
56-57	0.0	2.716791838322631E-5	0.0	3.397239522148917	0.0016164911438019654
58-59	0.0	2.716791838322631E-5	0.0	3.9127779413890194	0.0016300751029935787
60-61	0.0	2.716791838322631E-5	0.0	4.464993050446478	0.001657243021376805
62-63	1.3583959191613154E-5	2.716791838322631E-5	0.0	5.026852770529981	0.0016844109397600312
64-65	2.716791838322631E-5	2.716791838322631E-5	0.0	5.614943115812489	0.0017115788581432575
66-67	2.716791838322631E-5	2.716791838322631E-5	0.0	6.287145336409466	0.0017387467765264837
68-69	2.716791838322631E-5	2.716791838322631E-5	0.0	7.031478380313909	0.0017387467765264837
70-71	2.716791838322631E-5	2.716791838322631E-5	0.0	7.814444204159299	0.0018202505316761628
72-73	2.716791838322631E-5	2.716791838322631E-5	0.0	8.61273273397283	0.002010425960358747
74-75	2.716791838322631E-5	2.716791838322631E-5	0.0	9.414906276115161	0.002010425960358747
76-77	2.716791838322631E-5	2.716791838322631E-5	0.0	10.266280918449517	0.00202400991955036
78-79	2.716791838322631E-5	2.716791838322631E-5	0.0	11.197868839810347	0.0020783457563168127
80-81	2.716791838322631E-5	2.716791838322631E-5	0.0	12.17958157058823	0.002119097633891652
82-83	2.716791838322631E-5	5.433583676645262E-5	0.0	13.17237881206647	0.0021734334706581047
84-85	2.716791838322631E-5	5.433583676645262E-5	0.0	14.156047632967944	0.0021734334706581047
86	2.716791838322631E-5	5.433583676645262E-5	0.0	14.913611453125016	0.0021734334706581047
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7640	0.0	29.760824	1
GTACATG	10050	0.0	28.623669	1
TACATGG	10320	0.0	27.061527	2
ACATGGG	10670	0.0	25.871944	3
ATCAACG	8725	0.0	25.595316	3
TCAACGC	8735	0.0	25.566015	4
CAACGCA	8765	0.0	25.426195	5
AACGCAG	9235	0.0	24.126925	6
TATCAAC	9290	0.0	24.089003	2
GAGTACA	7575	0.0	21.691868	1
CATGGGG	7510	0.0	20.943594	4
AGTACAT	7220	0.0	20.769463	2
ACGCAGA	10760	0.0	20.365477	7
GGTATCA	5510	0.0	20.215063	1
GTGTAGC	2575	0.0	20.019451	1
CGCAGAG	10915	0.0	19.655468	8
AGAGTAC	8525	0.0	16.804163	10-11
GCAGAGT	12200	0.0	16.72097	9
TAGCCCT	2855	0.0	16.704144	4
GTATAGG	965	0.0	16.216717	1
>>END_MODULE