##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920902_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3680812
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.305063393620756	31.0	30.0	34.0	27.0	34.0
2	30.304925108916184	31.0	30.0	34.0	26.0	34.0
3	30.456351207287955	31.0	30.0	34.0	27.0	34.0
4	34.09191395811576	35.0	35.0	37.0	30.0	37.0
5	33.63379765117045	35.0	35.0	37.0	28.0	37.0
6	33.58451042867715	35.0	35.0	37.0	28.0	37.0
7	33.40531165405894	35.0	35.0	37.0	28.0	37.0
8	33.34262304078557	35.0	35.0	37.0	27.0	37.0
9	34.6803903595185	38.0	35.0	39.0	27.0	39.0
10-11	34.494477848909426	37.5	34.0	39.0	27.0	39.0
12-13	34.313174511493656	37.0	34.0	39.0	27.0	39.0
14-15	35.22123297794074	38.0	34.0	40.0	27.0	41.0
16-17	35.095107139402934	38.0	34.0	40.0	26.0	41.0
18-19	35.00879466269943	38.5	34.0	40.0	25.5	41.0
20-21	34.895219587417124	38.0	34.0	40.0	25.0	41.0
22-23	34.751608884126654	38.0	34.0	40.0	25.0	41.0
24-25	34.57677083208814	38.0	33.5	40.0	24.0	41.0
26-27	34.392147982564715	38.0	33.0	40.0	24.0	41.0
28-29	34.335405068229505	38.0	33.0	40.0	23.5	41.0
30-31	34.327055416033204	38.0	33.0	40.0	24.0	41.0
32-33	34.179547882369434	38.0	33.0	40.0	23.0	41.0
34-35	34.01029501099214	38.0	33.0	40.0	22.5	41.0
36-37	33.81580355095561	38.0	33.0	40.0	20.5	41.0
38-39	33.63359307674502	38.0	33.0	40.0	19.0	41.0
40-41	33.41103525526433	38.0	32.0	40.0	18.0	41.0
42-43	33.44573697325481	38.0	32.0	40.0	18.0	41.0
44-45	33.30614997451649	38.0	32.0	40.0	16.5	41.0
46-47	33.06143535719836	38.0	32.0	40.0	15.0	41.0
48-49	32.801152028411124	37.0	31.0	40.0	13.5	41.0
50-51	32.54516992446232	37.0	31.0	40.0	10.0	41.0
52-53	32.29199847750985	37.0	31.0	40.0	8.5	41.0
54-55	32.014900108997686	36.5	31.0	40.0	8.0	41.0
56-57	31.691209983014616	36.0	30.0	39.0	7.0	41.0
58-59	31.37225712696003	36.0	30.0	39.0	3.5	40.5
60-61	31.04091705851861	35.0	29.0	39.0	2.0	40.0
62-63	30.72554113059836	35.0	29.0	39.0	2.0	40.0
64-65	30.330041713621885	35.0	28.5	38.0	2.0	40.0
66-67	29.892900126385157	34.5	28.0	38.0	2.0	40.0
68-69	29.448192953076656	34.0	28.0	37.0	2.0	40.0
70-71	29.01634082370955	34.0	27.5	37.0	2.0	39.0
72-73	28.573318876378366	34.0	26.0	36.0	2.0	39.0
74-75	28.122065185616652	33.5	26.0	36.0	2.0	38.5
76-77	26.658210742629613	31.5	24.5	34.5	2.0	36.0
78-79	27.504184538628976	33.0	26.0	35.0	2.0	37.0
80-81	27.519176746869984	33.0	26.0	35.0	2.0	37.0
82-83	27.173737615504407	33.0	26.0	35.0	2.0	36.0
84-85	26.846604770903813	33.0	25.0	35.0	2.0	36.0
86-87	26.30521974499105	32.5	24.0	35.0	2.0	35.0
88-89	25.929441519968965	32.0	23.5	35.0	2.0	35.0
90-91	25.556262042179824	32.0	21.0	35.0	2.0	35.0
92-93	25.24015882908445	32.0	20.0	34.0	2.0	35.0
94-95	24.852830299401326	32.0	18.5	34.0	2.0	35.0
96-97	24.321689072954555	32.0	9.5	34.0	2.0	35.0
98-99	23.719946033646924	31.0	2.0	34.0	2.0	35.0
100-101	21.503717386272378	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81062.0
3	37019.0
4	20265.0
5	14161.0
6	12485.0
7	12235.0
8	13884.0
9	15988.0
10	18253.0
11	20768.0
12	23183.0
13	25153.0
14	27562.0
15	29513.0
16	31204.0
17	32407.0
18	31652.0
19	30206.0
20	30839.0
21	31911.0
22	34496.0
23	39458.0
24	45252.0
25	53613.0
26	63172.0
27	75242.0
28	88190.0
29	105334.0
30	123651.0
31	145869.0
32	174913.0
33	214105.0
34	269208.0
35	348648.0
36	464523.0
37	549732.0
38	322620.0
39	23035.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.390356089628604	18.719761724978753	12.490928857746523	25.39895332764612
2	16.46557879076682	20.58559361358309	37.63677688510035	25.312050710549737
3	20.40268799207036	23.564491376949263	28.700934254400646	27.331886376579735
4	13.806033414630967	15.825703528838922	35.84130550084993	34.526957555680184
5	14.358785007701226	36.81680320753532	34.328067048073635	14.496344736689812
6	34.143905667515305	35.004040124377624	16.710612594907566	14.141441613199499
7	29.602812459530902	31.150133146735648	20.418870815563206	18.828183578170247
8	26.81311411732353	34.52565110750907	19.753622719284238	18.907612055883163
9	26.457132409994006	15.748151888485392	19.2124427719333	38.582272929587305
10-11	25.88957131971829	25.305859731611257	27.445392123171004	21.359176825499446
12-13	26.4899490537366	22.38609711803154	27.348210407442274	23.77574342078959
14-15	23.285255704118583	24.277393994485053	24.88198187759516	27.5553684238012
16-17	23.662376940826253	27.189408330473135	25.585719188905387	23.562495539795222
18-19	23.48537404936317	26.51045912932875	26.984185247766806	23.01998157354127
20-21	24.251118304813243	25.767824728295214	26.690162912802528	23.290894054089016
22-23	23.63321396151343	25.716880098006257	26.6602487489207	23.98965719155962
24-25	23.586304083021243	25.90503423381203	26.829892192102967	23.67876949106376
26-27	23.508549816590605	26.05870568671071	26.733363545884153	23.69938095081453
28-29	23.470939295532087	26.339102319059148	26.662229298938932	23.52772908646983
30-31	23.815456264118858	26.08216376501765	26.514360778049735	23.588019192813757
32-33	23.524814948784627	26.05570301797151	26.475212227661437	23.944269805582422
34-35	23.868372806220233	26.0318478658718	26.580620582855836	23.51915874505213
36-37	23.82543122482116	25.913272688981433	26.610338525921044	23.650957560276364
38-39	23.68093074322488	26.010125990586037	26.615416182017803	23.69352708417128
40-41	23.80871051312539	25.868897047604023	26.69842831732683	23.62396412194376
42-43	23.7854247161947	25.79950493704386	26.503393708190004	23.911676638571436
44-45	23.602697230606097	25.87614680332628	26.566949699152016	23.95420626691561
46-47	23.5879597356986	25.895637200588002	26.54240815613274	23.97399490758066
48-49	23.53852979862187	25.918960768528308	26.51560702939202	24.0269024034578
50-51	23.444657096551953	26.00166320606949	26.613185574914038	23.940494122464518
52-53	23.599783184872933	25.88276768041776	26.54629532774269	23.971153806966612
54-55	23.310028535267495	25.7702647405497	26.72890789428637	24.19079882989644
56-57	23.31203627202169	25.814377581247573	26.51211082441526	24.36147532231548
58-59	23.26562571742877	25.698872409139728	26.65815193337968	24.377349940051822
60-61	23.223188881541308	25.7600434568701	26.790568022216483	24.22619963937211
62-63	23.041275194317638	25.759208877844326	26.709026603590925	24.490489324247108
64-65	22.973586498944993	25.667945101563845	26.619204454126592	24.739263945364566
66-67	22.934444051857692	25.772511075476313	26.48013209034327	24.812912782322723
68-69	23.158054312453984	25.61135331103115	26.456733380602536	24.773858995912324
70-71	22.9760370086152	25.429278958089224	26.472727776678745	25.121956256616834
72-73	23.04281554609275	25.412229448730848	26.50010151660646	25.044853488569935
74-75	22.83462800559925	25.635979199681536	26.346204463954564	25.183188330764654
76-77	22.886721484269675	25.365906138246036	26.542746018733133	25.20462635875116
78-79	22.88825359649465	25.432326383257497	26.44238056739384	25.23703945285401
80-81	22.905858052953022	25.485632737894115	26.373065012745826	25.23544419640704
82-83	22.930040230905536	25.481380384821726	26.328833752062796	25.259745632209942
84-85	22.759404532130155	25.452451542529797	26.312990839363415	25.475153085976626
86-87	22.655384153132513	25.392615426272553	26.372936677444414	25.57906374315052
88-89	22.650487324052246	25.28722607073642	26.277716888660578	25.784569716550752
90-91	22.598837967149425	25.26440081565951	26.282653996185402	25.854107221005666
92-93	22.7013330254335	25.176795894172066	26.244584886634836	25.877286193759602
94-95	22.514312572038307	25.10215600414859	26.367422011849428	26.016109411963672
96-97	22.44783239312618	24.986873959171188	26.44586481149239	26.11942883621024
98-99	22.426195204213123	25.055594210757825	26.262121032230652	26.2560895527984
100-101	22.601223270171978	24.848178000155407	26.343426362798066	26.20717236687455
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	111.0
1	75.5
2	78.0
3	121.0
4	106.5
5	113.5
6	207.0
7	366.0
8	570.0
9	653.0
10	577.5
11	503.5
12	501.5
13	544.5
14	596.5
15	657.0
16	729.5
17	833.5
18	967.5
19	1157.5
20	1392.0
21	1586.0
22	1886.5
23	2398.5
24	3034.0
25	3815.5
26	5060.5
27	7009.0
28	9223.5
29	12381.0
30	16666.5
31	22788.0
32	31163.0
33	38562.5
34	45519.0
35	53912.0
36	63606.0
37	73296.5
38	82397.5
39	90760.5
40	99376.5
41	108369.0
42	115702.0
43	124720.5
44	134479.0
45	142284.5
46	149332.5
47	157118.0
48	166330.0
49	171551.5
50	170775.0
51	168139.5
52	163160.0
53	157598.5
54	150824.5
55	140885.0
56	129415.5
57	117298.0
58	106278.0
59	92651.5
60	76207.0
61	61702.5
62	49733.0
63	37605.0
64	27312.0
65	20460.5
66	15061.0
67	11146.0
68	8486.5
69	6583.0
70	5206.0
71	4084.0
72	3531.5
73	3386.5
74	2750.5
75	2059.0
76	1413.0
77	806.5
78	460.5
79	267.5
80	154.0
81	112.0
82	55.0
83	25.0
84	14.0
85	12.0
86	7.5
87	4.5
88	3.0
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1921858546429429
2	0.0
3	0.012062555762152483
4	0.013801302538678966
5	0.0062214533097588245
6	0.10717743802182778
7	0.14040380220451357
8	0.12222846480613517
9	0.14040380220451357
10-11	0.11322229986209566
12-13	0.11391508178086791
14-15	0.11568099647577762
16-17	0.06654781607971284
18-19	0.08010460735294277
20-21	0.036921201082804556
22-23	0.08388094800821123
24-25	0.058112177421721076
26-27	0.0659908737528567
28-29	0.03948856937001945
30-31	0.024260951116221095
32-33	0.03137894573262639
34-35	0.01877303160280938
36-37	0.024097943605921737
38-39	0.03184080034514123
40-41	0.024519046340861748
42-43	0.012823257476882819
44-45	0.026176289362238547
46-47	0.03328070001945223
48-49	0.008775237637782098
50-51	0.015499297437630611
52-53	0.006982155024489161
54-55	0.012334234945984745
56-57	0.00486305739059751
58-59	0.003613333144969099
60-61	0.02286180331948494
62-63	0.010024961883410509
64-65	0.007335337963471104
66-67	0.041077892595438176
68-69	0.08950470711353908
70-71	0.021843006380113952
72-73	0.04376751651537759
74-75	0.08628530878512676
76-77	0.25108590169777756
78-79	0.07852886808671565
80-81	0.015961152050145457
82-83	0.011750124700745379
84-85	0.01210330763972732
86-87	0.26682971040085723
88-89	0.6242915965281574
90-91	0.2694378305656469
92-93	0.3518924628587388
94-95	0.0012089723680535707
96-97	0.004115939635058786
98-99	0.003341653961136836
100-101	0.005053232819280094
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3680812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.05837684889074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.50380199845357	31.55173928026569
2	13.34332692282712	12.291439596590422
3	6.242261416684741	8.625252861568693
4	3.5025687279922035	6.4529052165201914
5	2.2478017186130224	5.176504931873143
6	1.4247515569073161	3.937304647444858
7	0.9897430033797412	3.1910169363251946
8	0.7324963277603372	2.6990073523531355
9	0.49946328427370607	2.0704021352336692
>10	2.401684340806519	18.087038946699753
>50	0.07743902220303263	2.4360977030951316
>100	0.032883551676131696	2.7442383183242285
>500	0.0013632318009679776	0.44544163857339997
>1k	4.1489662157201705E-4	0.29161043513235396
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.716791838322631E-5
2	0.0	0.0	0.0	0.0	8.150375514967894E-5
3	0.0	0.0	0.0	0.0	8.150375514967894E-5
4	0.0	0.0	0.0	0.0	1.3583959191613155E-4
5	0.0	0.0	0.0	0.0	1.3583959191613155E-4
6	0.0	0.0	0.0	0.0	1.3583959191613155E-4
7	0.0	0.0	0.0	0.0	1.3583959191613155E-4
8	0.0	0.0	0.0	0.0	1.6300751029935787E-4
9	0.0	0.0	0.0	5.433583676645262E-5	1.6300751029935787E-4
10-11	0.0	0.0	0.0	6.791979595806577E-5	1.6300751029935787E-4
12-13	0.0	0.0	0.0	8.150375514967894E-5	1.6300751029935787E-4
14-15	0.0	0.0	0.0	1.0867167353290523E-4	2.716791838322631E-4
16-17	0.0	0.0	0.0	1.2225563272451838E-4	4.0751877574839466E-4
18-19	0.0	0.0	0.0	1.6300751029935787E-4	4.0751877574839466E-4
20-21	0.0	0.0	0.0	3.260150205987157E-4	4.34686694131621E-4
22-23	0.0	0.0	0.0	5.705262860477526E-4	5.026064900896868E-4
24-25	0.0	0.0	0.0	0.001045964857754213	6.248621228142051E-4
26-27	0.0	0.0	0.0	0.0015349873886522864	6.656140003890446E-4
28-29	0.0	0.0	0.0	0.00813679155577628	6.927819187722709E-4
30-31	0.0	0.0	0.0	0.034421752591547734	7.335337963471104E-4
32-33	0.0	0.0	0.0	0.08644831629542613	8.286215106884025E-4
34-35	0.0	0.0	0.0	0.1560525231932519	9.508771434129208E-4
36-37	0.0	0.0	0.0	0.26964158995352117	9.78045061796147E-4
38-39	0.0	0.0	0.0	0.45129987622296386	9.78045061796147E-4
40-41	0.0	0.0	0.0	0.6591752037322199	0.0010187969393709866
42-43	0.0	0.0	0.0	0.8803763952084486	0.001045964857754213
44-45	0.0	0.0	0.0	1.1487546769571497	0.001059548816945826
46-47	0.0	0.0	0.0	1.466143883469191	0.0010867167353290524
48-49	0.0	0.0	0.0	1.7984211092552405	0.001141052572095505
50-51	0.0	0.0	0.0	2.165554774326969	0.001141052572095505
52-53	0.0	0.0	0.0	2.5398607698518694	0.0012361402864367972
54-55	0.0	0.0	0.0	2.9541171893593043	0.0013312280007780892
56-57	0.0	0.0	0.0	3.431905786005914	0.0013448119599697023
58-59	0.0	0.0	0.0	3.95126129777886	0.0013719798783529288
60-61	0.0	0.0	0.0	4.507986281287933	0.0013855638375445418
62-63	0.0	0.0	0.0	5.072712216760867	0.0013855638375445418
64-65	0.0	0.0	0.0	5.6628673238405	0.0013855638375445418
66-67	0.0	0.0	0.0	6.336889794969153	0.0013855638375445418
68-69	0.0	0.0	0.0	7.084143390099793	0.0013855638375445418
70-71	0.0	0.0	0.0	7.870056933089764	0.0014398996743109943
72-73	0.0	0.0	0.0	8.673398695722575	0.0016300751029935787
74-75	0.0	0.0	0.0	9.485162513054185	0.0016300751029935787
76-77	0.0	1.3583959191613154E-5	0.0	10.347214147313148	0.001643659062185192
78-79	0.0	2.716791838322631E-5	0.0	11.290959712150471	0.001657243021376805
80-81	0.0	2.716791838322631E-5	0.0	12.28332226693458	0.001670826980568418
82-83	0.0	2.716791838322631E-5	0.0	13.289852891155538	0.0018202505316761628
84-85	0.0	2.716791838322631E-5	0.0	14.290651084597638	0.0018202505316761628
86-87	0.0	2.716791838322631E-5	0.0	15.329756042960087	0.0018745863684426153
88-89	0.0	2.716791838322631E-5	0.0	16.421186412128627	0.0018881703276342286
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	9930	0.0	29.019947	1
GTATCAA	8210	0.0	28.960102	1
TACATGG	10430	0.0	26.624517	2
ACATGGG	10725	0.0	25.406364	3
TCAACGC	9445	0.0	24.577341	4
CAACGCA	9470	0.0	24.508453	5
ATCAACG	9655	0.0	24.141108	3
AACGCAG	10080	0.0	23.213966	6
TATCAAC	10195	0.0	23.001171	2
CATGGGG	7420	0.0	20.66458	4
GAGTACA	7535	0.0	19.816166	1
GGTATCA	6040	0.0	19.761051	1
ACGCAGA	11840	0.0	19.442274	7
CGCAGAG	12065	0.0	19.03932	8
AGTACAT	7245	0.0	18.804152	2
AGAGTAC	8635	0.0	17.587467	10-11
TATACGA	190	1.8781821E-4	17.486443	5
TATACCG	220	2.9395023E-5	17.259346	5
GCAGAGT	13115	0.0	16.682915	9
GTATAAT	1315	0.0	16.63433	1
>>END_MODULE