FastQCFastQC Report
Mon 15 Aug 2016
SRR2920901_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920901_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3254826
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50200.041.987721
GGTATCA27650.038.7812041
ATCAACG59850.034.7878463
TCAACGC60000.034.7008744
CAACGCA60850.034.140785
TATCAAC62350.033.0267452
AACGCAG64300.032.5184336
GTACATG94700.028.137731
ACGCAGA77050.026.3596767
TACATGG98450.025.9474892
CGCAGAG79750.025.2928438
ACATGGG101850.024.5398163
CATGGGG67750.022.4731834
GCAGAGT91750.021.5347679
GAGTACA68900.021.3742681
GTATAGG8250.020.6399331
AGTACAT69150.019.4326172
GTGTAGC18500.018.6573521
AGAGTAC77900.017.59689710-11
CAGAGTA86900.016.5921710-11