##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920901_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3254826
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.191584127692234	31.0	28.0	31.0	16.0	34.0
2	28.19964323745724	31.0	28.0	33.0	16.0	34.0
3	28.282170844155726	31.0	28.0	34.0	16.0	34.0
4	31.718438405002296	35.0	32.0	37.0	19.0	37.0
5	31.67277390557898	35.0	32.0	37.0	19.0	37.0
6	31.795352808414336	35.0	32.0	37.0	19.0	37.0
7	31.617910757748646	35.0	32.0	37.0	18.0	37.0
8	31.560869306070433	35.0	32.0	37.0	17.0	37.0
9	32.7841921503638	37.0	32.0	39.0	17.0	39.0
10-11	32.57367567421423	37.0	32.0	39.0	17.0	39.0
12-13	32.390605826548025	37.0	32.0	39.0	16.0	39.0
14-15	33.11047764150833	38.0	32.0	40.0	14.5	41.0
16-17	32.99184718322884	38.0	32.0	40.0	11.0	41.0
18-19	32.8780089934147	38.0	32.0	40.0	10.0	41.0
20-21	32.77562656191145	38.0	32.0	40.0	10.0	41.0
22-23	32.54934150089744	38.0	31.0	40.0	10.0	41.0
24-25	32.3525277541718	37.0	31.0	40.0	9.5	41.0
26-27	32.382504779057314	38.0	31.0	40.0	8.5	41.0
28-29	32.21515819893291	37.0	31.0	40.0	8.0	41.0
30-31	31.95938891971491	37.0	30.0	40.0	5.0	41.0
32-33	31.703419629805097	37.0	30.0	40.0	2.0	41.0
34-35	31.3684158538736	37.0	30.0	40.0	2.0	41.0
36-37	31.05351238437938	37.0	30.0	40.0	2.0	41.0
38-39	30.8397969661051	36.0	29.0	40.0	2.0	41.0
40-41	30.637207795439757	36.0	29.0	39.5	2.0	41.0
42-43	30.394830015490843	36.0	28.0	39.0	2.0	40.5
44-45	30.108644671020816	36.0	27.5	39.0	2.0	40.0
46-47	29.841958064732182	35.0	27.0	39.0	2.0	40.0
48-49	29.533438346627438	35.0	26.5	39.0	2.0	40.0
50-51	28.43173813285257	34.0	25.0	38.0	2.0	39.5
52-53	28.886350606760544	34.5	26.0	38.5	2.0	39.5
54-55	29.514164044406677	35.0	27.0	39.0	2.0	40.0
56-57	29.4520310763156	35.0	27.0	39.0	2.0	40.0
58-59	29.21122542341741	35.0	26.0	39.0	2.0	40.0
60-61	28.881711956338066	35.0	26.0	39.0	2.0	40.0
62-63	28.484748493467855	34.0	26.0	38.0	2.0	40.0
64-65	28.062461557084774	34.0	24.5	38.0	2.0	40.0
66-67	27.673691619767077	34.0	23.5	37.5	2.0	40.0
68-69	27.225597159418044	33.5	22.0	37.0	2.0	39.5
70-71	26.896176016782462	33.0	22.0	36.5	2.0	39.0
72-73	26.42861093035388	33.0	20.5	36.0	2.0	39.0
74-75	25.980568546521383	32.5	19.5	35.5	2.0	38.5
76-77	25.558177917959362	32.0	18.0	35.0	2.0	37.5
78-79	25.09607057335784	32.0	14.0	35.0	2.0	37.0
80-81	24.634368626771447	31.5	7.5	35.0	2.0	36.0
82-83	24.144253333357913	31.0	4.5	35.0	2.0	36.0
84-85	23.724062054315652	31.0	2.0	34.0	2.0	35.5
86-87	23.25426658752265	31.0	2.0	34.0	2.0	35.0
88-89	22.703310714612698	30.0	2.0	34.0	2.0	35.0
90-91	22.17780351392056	30.0	2.0	34.0	2.0	35.0
92-93	21.6149625202699	29.5	2.0	34.0	2.0	35.0
94-95	20.960380063327502	29.0	2.0	34.0	2.0	35.0
96-97	20.30340807772827	29.0	2.0	34.0	2.0	35.0
98	19.890383080385863	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	196330.0
3	32485.0
4	23387.0
5	18539.0
6	17333.0
7	17282.0
8	20535.0
9	23167.0
10	25355.0
11	27159.0
12	27677.0
13	27066.0
14	26339.0
15	27691.0
16	30026.0
17	31748.0
18	32693.0
19	32210.0
20	33068.0
21	34894.0
22	38560.0
23	42713.0
24	48082.0
25	54206.0
26	62618.0
27	72434.0
28	83131.0
29	94957.0
30	109944.0
31	128552.0
32	151436.0
33	182831.0
34	224630.0
35	280788.0
36	352252.0
37	377284.0
38	222230.0
39	23192.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.10390799676881	17.52314130111929	11.967069969375233	25.40588073273667
2	16.17929380078924	19.260998195765165	39.16246749012219	25.397240513323403
3	20.50371749782205	22.786640669533725	28.304547500848905	28.405094331795322
4	13.284001649510893	14.779408646657435	36.33920433838573	35.597385365445945
5	14.358751995948484	36.61331640285523	34.24829414779914	14.779637453397138
6	34.18276111199718	34.603618296209085	16.874114379118108	14.339506212675623
7	29.77337885217541	31.017936103591214	20.29557543334573	18.91310961088765
8	27.03417718796378	34.49548472734759	19.531726742274564	18.938611342414067
9	26.77676164563021	15.780567071788168	19.183483233819565	38.259188048762056
10-11	25.995905771921446	25.20809100087071	27.229535465183087	21.56646776202476
12-13	26.47083991175076	22.29580763865066	27.38525593647603	23.84809651312255
14-15	23.243762406019197	24.062941810210738	24.809488249451835	27.883807534318233
16-17	23.587431531966395	27.209896328942758	25.54575478988183	23.656917349209017
18-19	23.486795851079606	26.354480820194752	27.079181864493908	23.07954146423174
20-21	24.0652928617584	25.697214677349255	27.036166346653356	23.201326114238984
22-23	23.712827325966266	25.49436321601534	26.842409159091623	23.95040029892677
24-25	23.454641095634354	25.723547056883056	26.989452733136964	23.832359114345632
26-27	23.35089636652292	26.07614637955184	26.81517430079291	23.757782953132327
28-29	23.43011007543612	26.114165261019917	26.844163960662183	23.611560702881786
30-31	23.657895210073686	26.015870666471773	26.65135515768994	23.67487896576459
32-33	23.373932523500613	26.002436873304095	26.667168586767133	23.95646201642815
34-35	23.749631965864932	26.06782310314051	26.62794408039253	23.554600850602032
36-37	23.894002519734265	25.836403210607223	26.585451206325097	23.68414306333342
38-39	23.685707401307944	25.96702369362601	26.630581675223038	23.716687229843007
40-41	23.8130023492493	25.885615116457494	26.62111014610793	23.680272388185273
42-43	23.693854340714115	25.703880090928855	26.70801348719378	23.894252081163245
44-45	23.61713089580258	25.864897675229336	26.74306895642154	23.77490247254654
46-47	23.60541297379293	25.862608052083115	26.595601422765135	23.93637755135882
48-49	23.57576274503846	25.870872980631056	26.56168567613626	23.99167859819422
50-51	23.473418249422323	26.070378581231125	26.578820167514582	23.87738300183197
52-53	23.545171271294613	25.91705326857568	26.6438869777209	23.89388848240881
54-55	23.299161934957365	25.784960633907065	26.955263895422128	23.96061353571345
56-57	23.335199123181628	25.90969597705264	26.778908222887626	23.97619667687811
58-59	23.18806285113118	25.736060420015367	26.883975923314956	24.191900805538502
60-61	23.241382619397356	25.669188177108555	27.03654613907438	24.052883064419703
62-63	23.144120902216205	25.685072710234962	27.005279252806414	24.16552713474242
64-65	23.030547802336695	25.644786076491272	26.966057002851656	24.35860911832038
66-67	23.004292415230665	25.649249413898858	26.921090441411188	24.425367729459293
68-69	23.24644107786857	25.50282786922549	26.856650687764393	24.394080365141548
70-71	23.126083847005752	25.374039977165776	26.896753462836248	24.603122712992224
72-73	23.205166255906047	25.361725024996396	26.786808686796892	24.646300032300665
74-75	23.052776823315178	25.469568623497413	26.849482609331698	24.62817194385571
76-77	23.152806025633794	25.256789582221533	26.986236627164374	24.604167764980303
78-79	23.13367368441011	25.305666230373152	26.916602065321328	24.644058019895414
80-81	23.203000973919703	25.305276881039497	26.843937423076607	24.647784721964193
82-83	23.222726822844482	25.29526536343424	26.890245613299417	24.591762200421858
84-85	23.252428015171887	25.1183116366357	26.912538420352146	24.71672192784027
86-87	23.168681588857222	25.043923402641727	27.085748902107614	24.70164610639344
88-89	23.252689519337043	24.984119880521664	26.875376955251756	24.88781364488953
90-91	23.253110444064582	25.033769037820242	26.945418041492943	24.767702476622237
92-93	23.44031275374281	24.76512867915994	26.970924995494585	24.823633571602667
94-95	23.356637340598862	24.643902887258886	27.06788246713922	24.931577305003028
96-97	23.50968663792977	24.427696758797097	27.039178391425615	25.023438211847516
98	23.557060223726893	24.325335158668494	26.92924359591026	25.188361021694355
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	77.0
1	62.0
2	77.0
3	130.5
4	148.5
5	172.0
6	316.0
7	545.5
8	780.5
9	939.0
10	1010.5
11	1125.5
12	1284.5
13	1437.5
14	1607.5
15	1809.0
16	1933.5
17	1974.5
18	2056.5
19	2164.0
20	2243.5
21	2346.0
22	2686.5
23	3293.5
24	3994.5
25	5095.0
26	6720.0
27	8762.0
28	11631.0
29	15409.5
30	20831.0
31	28683.0
32	35990.5
33	42441.0
34	50055.5
35	58128.5
36	66880.0
37	75125.0
38	81839.5
39	88588.0
40	95525.5
41	101230.0
42	108019.0
43	116413.5
44	123605.0
45	129583.5
46	135127.5
47	139386.0
48	141912.5
49	143179.0
50	143417.0
51	141937.0
52	139127.0
53	138082.5
54	135100.5
55	127833.0
56	116883.5
57	105103.5
58	94553.5
59	82200.5
60	69273.0
61	56943.0
62	45932.5
63	35104.0
64	25336.5
65	18804.5
66	14436.5
67	11069.5
68	8352.5
69	6369.5
70	4687.5
71	3496.5
72	2768.5
73	2157.0
74	1653.5
75	1203.5
76	791.5
77	496.0
78	303.5
79	178.5
80	113.5
81	66.5
82	35.0
83	23.0
84	17.0
85	10.5
86	10.0
87	8.0
88	5.0
89	3.5
90	2.0
91	3.0
92	2.0
93	1.0
94	4.0
95	4.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.38954463310788345
2	0.025039740987690278
3	0.019079360924362777
4	0.01622206532699444
5	0.02255112869320818
6	0.023134877256111387
7	0.015238909852631138
8	0.00970866030933758
9	0.0
10-11	0.0
12-13	0.005545611347580485
14-15	0.003241340704541502
16-17	0.006298339757639887
18-19	0.037129480961501475
20-21	0.0804190454420605
22-23	0.09811584398059989
24-25	0.06559490430517637
26-27	0.07513458476735776
28-29	0.05760676607597457
30-31	0.0527677977255927
32-33	0.020154687224447635
34-35	0.34536408397868273
36-37	0.5279237661245179
38-39	0.4799488513364462
40-41	0.48908912488716755
42-43	0.43844125615317076
44-45	0.41264878675542105
46-47	0.41143520421675384
48-49	0.40138858421310386
50-51	0.39814724350856234
52-53	0.45936403359196465
54-55	0.46030110365346716
56-57	0.43068354498827277
58-59	0.4152602934841985
60-61	0.4294392388410317
62-63	0.41057494317668597
64-65	0.4594101190048255
66-67	0.48002566035788086
68-69	0.4234174115605565
70-71	0.3786684756727395
72-73	0.364335912273037
74-75	0.34688490260308846
76-77	0.34462671737291023
78-79	0.34454990835147564
80-81	0.36086414450419163
82-83	0.3550727442880203
84-85	0.3561019851752444
86-87	0.34137001486408186
88-89	0.38602677992617734
90-91	0.36378288731870767
92-93	0.3535519256636146
94-95	0.36100240074277395
96-97	0.3602189487241407
98	0.35596372893666206
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3254826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.7811858646498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6893140793235	36.185602545567086
2	11.602613212551779	11.551836896993633
3	5.41420418368066	8.085765143309144
4	3.034771044738962	6.042980057392308
5	1.8492795666411246	4.602966491133042
6	1.2366465763243517	3.6937039838931236
7	0.8546770839408451	2.9782787138941273
8	0.6315569001773377	2.5151721145464103
9	0.46299901391468223	2.0743775970152734
>10	2.128945804346503	17.46005116891092
>50	0.06956001868048	2.3358931037139685
>100	0.024375249189027275	1.9853498922201425
>500	7.463057585655093E-4	0.25764902059033634
>1k	3.109607322261778E-4	0.23037327082049233
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.144721714770621E-5	0.0
5	0.0	0.0	0.0	6.144721714770621E-5	0.0
6	0.0	0.0	0.0	6.144721714770621E-5	0.0
7	0.0	0.0	0.0	6.144721714770621E-5	0.0
8	0.0	0.0	0.0	9.21708257215593E-5	0.0
9	0.0	0.0	0.0	9.21708257215593E-5	0.0
10-11	0.0	0.0	0.0	1.2289443429541242E-4	0.0
12-13	0.0	0.0	0.0	1.2289443429541242E-4	0.0
14-15	0.0	0.0	0.0	1.2289443429541242E-4	0.0
16-17	0.0	0.0	0.0	1.2289443429541242E-4	0.0
18-19	0.0	0.0	0.0	1.2289443429541242E-4	0.0
20-21	0.0	0.0	0.0	2.1506526001697173E-4	0.0
22-23	0.0	4.608541286077966E-5	0.0	5.223013457555028E-4	0.0
24-25	0.0	7.680902143463276E-5	0.0	9.831554743632994E-4	0.0
26-27	0.0	9.21708257215593E-5	0.0	0.0016129894501272879	0.0
28-29	0.0	1.0753263000848586E-4	0.0	0.006667023060526123	0.0
30-31	0.0	1.2289443429541242E-4	0.0	0.02917206634087352	0.0
32-33	0.0	1.2289443429541242E-4	0.0	0.07175498782423392	1.5361804286926553E-5
34-35	0.0	1.3825623858233896E-4	0.0	0.12767195542864657	3.0723608573853106E-5
36-37	0.0	1.5361804286926552E-4	0.0	0.22004248460593595	3.0723608573853106E-5
38-39	0.0	1.5361804286926552E-4	0.0	0.372078261633648	3.0723608573853106E-5
40-41	0.0	1.6897984715619208E-4	0.0	0.552164693289288	3.0723608573853106E-5
42-43	0.0	1.843416514431186E-4	0.0	0.736199108646668	3.0723608573853106E-5
44-45	0.0	1.843416514431186E-4	0.0	0.9581771805927568	3.0723608573853106E-5
46-47	0.0	1.843416514431186E-4	0.0	1.2217550185478425	3.0723608573853106E-5
48-49	1.5361804286926553E-5	1.843416514431186E-4	0.0	1.5139672596937594	3.0723608573853106E-5
50-51	3.0723608573853106E-5	1.843416514431186E-4	0.0	1.8393609980994376	3.0723608573853106E-5
52-53	3.0723608573853106E-5	1.843416514431186E-4	0.0	2.172635342104309	3.0723608573853106E-5
54-55	3.0723608573853106E-5	1.9970345573004517E-4	0.0	2.5339603407371083	3.0723608573853106E-5
56-57	3.0723608573853106E-5	2.1506526001697173E-4	0.0	2.9591597215949488	3.0723608573853106E-5
58-59	3.0723608573853106E-5	2.1506526001697173E-4	0.0	3.4210584528942563	3.0723608573853106E-5
60-61	3.0723608573853106E-5	2.1506526001697173E-4	0.0	3.9217303782137662	3.0723608573853106E-5
62-63	3.0723608573853106E-5	2.4578886859082485E-4	0.0	4.427947914880857	3.0723608573853106E-5
64-65	6.144721714770621E-5	2.9187428145160444E-4	0.0	4.961079332658643	3.0723608573853106E-5
66-67	6.144721714770621E-5	3.0723608573853103E-4	0.0	5.564644623091987	3.0723608573853106E-5
68-69	6.144721714770621E-5	3.0723608573853103E-4	0.0	6.230548115321679	3.0723608573853106E-5
70-71	6.144721714770621E-5	3.0723608573853103E-4	0.0	6.942460211390717	3.0723608573853106E-5
72-73	6.144721714770621E-5	3.3795969431238415E-4	0.0	7.670701905416757	3.0723608573853106E-5
74-75	6.144721714770621E-5	3.3795969431238415E-4	0.0	8.394980253936769	3.0723608573853106E-5
76-77	6.144721714770621E-5	3.686833028862372E-4	0.0	9.170720646817987	3.0723608573853106E-5
78-79	6.144721714770621E-5	4.3013052003394346E-4	0.0	10.016418696421868	3.0723608573853106E-5
80-81	6.144721714770621E-5	4.4549232432087E-4	0.0	10.91308721264977	3.0723608573853106E-5
82-83	6.144721714770621E-5	4.608541286077965E-4	0.0	11.829034793257765	3.0723608573853106E-5
84-85	6.144721714770621E-5	4.608541286077965E-4	0.0	12.725657224072808	3.0723608573853106E-5
86	6.144721714770621E-5	4.608541286077965E-4	0.0	13.415770919858696	3.0723608573853106E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5020	0.0	41.98772	1
GGTATCA	2765	0.0	38.781204	1
ATCAACG	5985	0.0	34.787846	3
TCAACGC	6000	0.0	34.700874	4
CAACGCA	6085	0.0	34.14078	5
TATCAAC	6235	0.0	33.026745	2
AACGCAG	6430	0.0	32.518433	6
GTACATG	9470	0.0	28.13773	1
ACGCAGA	7705	0.0	26.359676	7
TACATGG	9845	0.0	25.947489	2
CGCAGAG	7975	0.0	25.292843	8
ACATGGG	10185	0.0	24.539816	3
CATGGGG	6775	0.0	22.473183	4
GCAGAGT	9175	0.0	21.534767	9
GAGTACA	6890	0.0	21.374268	1
GTATAGG	825	0.0	20.639933	1
AGTACAT	6915	0.0	19.432617	2
GTGTAGC	1850	0.0	18.657352	1
AGAGTAC	7790	0.0	17.596897	10-11
CAGAGTA	8690	0.0	16.59217	10-11
>>END_MODULE