##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920901_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3254826
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.21170993472462	31.0	30.0	34.0	26.0	34.0
2	30.21936287838428	31.0	30.0	34.0	26.0	34.0
3	30.36494976997234	31.0	30.0	34.0	26.0	34.0
4	33.99816364991554	35.0	35.0	37.0	30.0	37.0
5	33.52302242884873	35.0	35.0	37.0	28.0	37.0
6	33.46784712915529	35.0	35.0	37.0	28.0	37.0
7	33.283653565505496	35.0	33.0	37.0	27.0	37.0
8	33.21220919336395	35.0	33.0	37.0	27.0	37.0
9	34.5437992691468	38.0	34.0	39.0	27.0	39.0
10-11	34.35373549922484	37.5	34.0	39.0	27.0	39.0
12-13	34.17132052527539	37.0	34.0	39.0	26.0	39.0
14-15	35.074157420396666	38.0	34.0	40.0	26.0	41.0
16-17	34.9537575894994	38.0	34.0	40.0	25.0	41.0
18-19	34.863932357674415	38.5	34.0	40.0	25.0	41.0
20-21	34.753031652076025	38.0	34.0	40.0	25.0	41.0
22-23	34.606532422931366	38.0	34.0	40.0	24.0	41.0
24-25	34.43631011304444	38.0	33.0	40.0	24.0	41.0
26-27	34.249604433539616	38.0	33.0	40.0	23.0	41.0
28-29	34.192751163963905	38.0	33.0	40.0	22.5	41.0
30-31	34.179779502805985	38.0	33.0	40.0	22.5	41.0
32-33	34.03131811039975	38.0	33.0	40.0	22.0	41.0
34-35	33.859037472356434	38.0	33.0	40.0	20.0	41.0
36-37	33.663363878744974	38.0	33.0	40.0	18.5	41.0
38-39	33.47194320065036	38.0	32.0	40.0	18.0	41.0
40-41	33.245327399990046	38.0	32.0	40.0	16.0	41.0
42-43	33.26684360392844	38.0	32.0	40.0	15.5	41.0
44-45	33.1171932078704	38.0	32.0	40.0	15.0	41.0
46-47	32.86720442198754	38.0	31.5	40.0	13.0	41.0
48-49	32.599958031550685	37.0	31.0	40.0	9.0	41.0
50-51	32.3351317397612	37.0	31.0	40.0	8.5	41.0
52-53	32.080861465405526	37.0	31.0	40.0	7.5	41.0
54-55	31.798177997840746	36.5	30.0	40.0	6.5	41.0
56-57	31.47036508249596	36.0	30.0	39.0	2.0	41.0
58-59	31.137318707666708	36.0	29.0	39.0	2.0	40.5
60-61	30.811903155498943	35.0	29.0	39.0	2.0	40.0
62-63	30.491990662480884	35.0	28.5	39.0	2.0	40.0
64-65	30.09248528185531	35.0	28.0	38.0	2.0	40.0
66-67	29.662133398221595	34.0	28.0	38.0	2.0	40.0
68-69	29.22082701195087	34.0	28.0	37.0	2.0	39.5
70-71	28.791049198943355	34.0	26.5	36.5	2.0	39.0
72-73	28.34649624895463	34.0	26.0	36.0	2.0	39.0
74-75	27.90254056591658	33.0	26.0	36.0	2.0	38.5
76-77	26.46374153334157	31.5	24.0	34.5	2.0	36.0
78-79	27.298024226179834	33.0	26.0	35.0	2.0	37.0
80-81	27.31831916667742	33.0	26.0	35.0	2.0	37.0
82-83	26.972287765920512	33.0	25.0	35.0	2.0	36.0
84-85	26.643492616809624	33.0	25.0	35.0	2.0	36.0
86-87	26.093850792638378	32.0	23.5	35.0	2.0	35.0
88-89	25.718795874188054	32.0	22.0	35.0	2.0	35.0
90-91	25.33846494405538	32.0	20.0	34.5	2.0	35.0
92-93	25.021665520676066	32.0	19.0	34.0	2.0	35.0
94-95	24.62655991441632	32.0	16.5	34.0	2.0	35.0
96-97	24.085413321633784	31.0	4.5	34.0	2.0	35.0
98-99	23.477664090184852	31.0	2.0	34.0	2.0	35.0
100-101	21.28599716236751	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80671.0
3	35487.0
4	18585.0
5	12990.0
6	11237.0
7	11262.0
8	12919.0
9	14661.0
10	16700.0
11	18526.0
12	20882.0
13	23013.0
14	24956.0
15	26781.0
16	28096.0
17	29618.0
18	28637.0
19	27220.0
20	27394.0
21	28277.0
22	30618.0
23	34695.0
24	39871.0
25	47095.0
26	56097.0
27	66512.0
28	78122.0
29	92305.0
30	109235.0
31	128728.0
32	154337.0
33	188395.0
34	235669.0
35	305947.0
36	407716.0
37	478602.0
38	281137.0
39	21833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.285191543618666	18.832216058406743	12.620635370655457	25.26195702731913
2	16.316048845621857	20.28861143422106	38.02276988078626	25.37256983937083
3	20.26387359779894	23.622549768208756	28.615548557246612	27.498028076745683
4	13.751781054101334	15.711777895294146	35.957150841954245	34.57929020865028
5	14.294247218643546	36.86363095872725	34.30763128196037	14.53449054066884
6	34.073391967186694	35.1530004022927	16.62772916228389	14.145878468236717
7	29.66030565353195	31.204233810922105	20.3556009815963	18.779859553949645
8	26.950568194751973	34.917476256774435	19.430070302218947	18.70188524625464
9	26.537095352447	15.7782047593921	19.142484858647375	38.54221502951352
10-11	25.956438911881712	25.358017221982905	27.30356824561757	21.381975620517814
12-13	26.445871090636906	22.356455603070557	27.46611416252222	23.731559143770316
14-15	23.175950219772446	24.138781147246	24.871719946233416	27.813548686748135
16-17	23.554788876131962	27.366115437346732	25.617460204977434	23.46163548154387
18-19	23.451261244599372	26.489772493695586	27.083217068868688	22.975749192836357
20-21	24.017336257030003	25.78132791745623	27.02673172398881	23.174604101524952
22-23	23.697640927307546	25.64815268930843	26.87371248496954	23.780493898414477
24-25	23.431487058368567	25.863788593301063	26.99400355271019	23.71072079562018
26-27	23.32052508046726	26.16600473357609	26.854566815685516	23.65890337027113
28-29	23.468753126393192	26.2189033853642	26.885467702274084	23.426875785968523
30-31	23.69746566625195	26.013732730945634	26.679779554742623	23.609022048059796
32-33	23.34564602930153	26.09585708872302	26.68777619434124	23.870720687634208
34-35	23.674153406907525	26.117813867137773	26.70158693039143	23.50644579556327
36-37	23.83517471130882	25.913591399243924	26.69457454342518	23.556659346022073
38-39	23.591406504391117	26.02915095996618	26.735912144204914	23.643530391437793
40-41	23.792966905516177	25.892612526453966	26.717028249679664	23.59739231835019
42-43	23.635546839831257	25.82656582674712	26.741512027259102	23.79637530616252
44-45	23.51754398904484	25.948819173495203	26.83423929745371	23.699397540006245
46-47	23.53257462136113	25.963577522619985	26.63324891817467	23.870598937844218
48-49	23.43815365470516	25.996888958876646	26.62822290417609	23.9367344822421
50-51	23.432337403042563	26.112245924849297	26.597015819704684	23.858400852403452
52-53	23.555890943017605	25.92737287610914	26.567372654884508	23.949363525988748
54-55	23.33797983389055	25.871226536337268	26.813160745515212	23.977632884256966
56-57	23.27153988435044	26.053704534421414	26.592885278339367	24.08187030288878
58-59	23.160052422561733	25.893694529351425	26.699666068097404	24.246586979989434
60-61	23.189031989518394	25.84024094049273	26.84113781415739	24.129589255831483
62-63	23.045844153629435	25.847570443757224	26.765286211203065	24.341299191410275
64-65	22.881776199152583	25.80524735834621	26.713733527110943	24.599242915390267
66-67	22.869301918940305	25.872338149778678	26.657482825248373	24.60087710603264
68-69	23.08938419329623	25.756082020638615	26.60034157840713	24.554192207658023
70-71	22.909077276071972	25.63229236433746	26.531149709223488	24.92748065036708
72-73	23.000565253594154	25.69879821368799	26.381467181941886	24.91916935077597
74-75	22.78574818726633	25.860103900185223	26.336509111830075	25.017638800718373
76-77	22.851619438903338	25.593068398314532	26.58198158712451	24.973330575657616
78-79	22.81080878307023	25.718819803956627	26.437274469176682	25.033096943796462
80-81	22.743137792050895	25.853123483252805	26.31617358186874	25.087565142827557
82-83	22.69655819590125	25.85178598205046	26.327364696281037	25.124291125767257
84-85	22.60212664949	25.760052113820763	26.2280471298221	25.409774106867133
86-87	22.486087429320445	25.71149340837573	26.383809818662947	25.41860934364088
88-89	22.490748444573065	25.740410429267847	26.145518642089023	25.623322484070073
90-91	22.398165442282995	25.872956754873428	26.136732958054886	25.592144844788688
92-93	22.41135431863497	25.74445999661138	26.16930189304936	25.67488379170429
94-95	22.18517938177271	25.618473081212613	26.16881580685697	26.027531730157705
96-97	22.25852370488354	25.43463459477529	26.221453484942337	26.085388215398837
98-99	22.16397500299182	25.502229922516896	26.134867028839288	26.198928045651996
100-101	22.249567657907985	25.47703574862662	26.134985366351255	26.13841122711414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	69.0
1	45.5
2	58.5
3	106.5
4	94.0
5	92.0
6	172.5
7	338.5
8	564.0
9	651.5
10	539.5
11	480.0
12	530.5
13	589.5
14	664.0
15	741.0
16	823.5
17	897.5
18	1015.5
19	1161.0
20	1340.0
21	1619.0
22	1984.0
23	2536.0
24	3296.5
25	4314.5
26	5617.5
27	7459.0
28	9876.5
29	13124.5
30	17467.0
31	23714.0
32	31495.0
33	37884.5
34	44221.5
35	51550.5
36	59835.0
37	68303.5
38	75757.5
39	82475.5
40	89172.0
41	96325.0
42	101534.5
43	107464.5
44	116006.5
45	123812.5
46	129932.5
47	134649.5
48	138488.0
49	141703.5
50	142866.0
51	141454.5
52	138701.0
53	136513.0
54	133424.5
55	126271.5
56	115603.0
57	103745.0
58	92974.0
59	81850.5
60	68922.5
61	56666.5
62	45885.0
63	34879.0
64	25337.0
65	18844.5
66	14317.0
67	11095.0
68	8552.5
69	6348.0
70	4659.0
71	3534.0
72	2710.5
73	2225.0
74	1657.0
75	1197.5
76	871.5
77	521.0
78	290.5
79	161.5
80	89.5
81	51.5
82	24.5
83	10.5
84	4.0
85	3.0
86	2.5
87	1.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.193466563189553
2	0.0
3	0.011951483735228858
4	0.013303322512478392
5	0.006421234191935298
6	0.10636513288267944
7	0.1416358355254628
8	0.12301732872970783
9	0.1419737952197752
10-11	0.11338547744180487
12-13	0.1133393920289441
14-15	0.1147834016319152
16-17	0.06533375363229862
18-19	0.07811477479902151
20-21	0.03633066713858129
22-23	0.0823239091736394
24-25	0.055809434974404167
26-27	0.0646885578522477
28-29	0.03921868634452348
30-31	0.023826158449023083
32-33	0.030815779399574663
34-35	0.019985707377291443
36-37	0.024164118143335467
38-39	0.0319371911125203
40-41	0.02533161526914188
42-43	0.013118980861035275
44-45	0.025623489550593487
46-47	0.03275136673972741
48-49	0.00886376107355662
50-51	0.015315718874065772
52-53	0.0066209376476653444
54-55	0.012458423276697432
56-57	0.005100119023259615
58-59	0.003702194833149299
60-61	0.022873726583233635
62-63	0.010215599850806157
64-65	0.007250771623429333
66-67	0.039495198821688164
68-69	0.0879924149555153
70-71	0.02133754615454098
72-73	0.043596800566297556
74-75	0.0848739686852692
76-77	0.24979829950971263
78-79	0.08103351761353757
80-81	0.016421768782724486
82-83	0.011367735172325647
84-85	0.012627403123853626
86-87	0.26609717385814174
88-89	0.6299875938068579
90-91	0.26738756541824354
92-93	0.3569929698238861
94-95	0.0011060499086587116
96-97	0.004239857983191728
98-99	0.0035793003988538862
100-101	0.005207651653268101
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3254826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.669345802011264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.99901877442142	31.51140048408519
2	12.942456039857372	11.821470008231511
3	6.081014977962717	8.331479275673678
4	3.40035128219824	6.211672742200952
5	2.1161911886326594	4.832253358841708
6	1.4457590457108274	3.9616121882972153
7	1.005651254904827	3.214920444153259
8	0.7503140221979003	2.741308043188309
9	0.5519247283428046	2.26854371478318
>10	2.586759659491205	19.57851513258532
>50	0.0888977476168594	2.7309063850042405
>100	0.030376665859210925	2.276155703140371
>500	9.465568033712839E-4	0.2997905487695163
>1k	3.3805600068384425E-4	0.21997197104558366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	1.5361804286926553E-5	0.0	3.0723608573853106E-5	0.0
18-19	0.0	4.608541286077966E-5	0.0	3.0723608573853106E-5	0.0
20-21	0.0	6.144721714770621E-5	0.0	1.2289443429541242E-4	0.0
22-23	0.0	6.144721714770621E-5	0.0	4.4549232432087E-4	0.0
24-25	0.0	6.144721714770621E-5	0.0	9.677936700763727E-4	0.0
26-27	0.0	1.2289443429541242E-4	0.0	0.0016283512544142146	0.0
28-29	0.0	1.2289443429541242E-4	0.0	0.006759193886247683	0.0
30-31	0.0	1.843416514431186E-4	0.0	0.029463940622325126	0.0
32-33	0.0	1.843416514431186E-4	0.0	0.0728917613414665	0.0
34-35	0.0	1.843416514431186E-4	0.0	0.12974579900738165	0.0
36-37	0.0	1.843416514431186E-4	0.0	0.22243892607469645	0.0
38-39	0.0	1.9970345573004517E-4	0.0	0.3766099938982913	0.0
40-41	0.0	2.1506526001697173E-4	0.0	0.5590006961969702	0.0
42-43	0.0	2.1506526001697173E-4	0.0	0.7470138188646643	1.5361804286926553E-5
44-45	0.0	2.1506526001697173E-4	0.0	0.9736926029225526	3.0723608573853106E-5
46-47	0.0	2.304270643038983E-4	0.0	1.2405271433864669	3.0723608573853106E-5
48-49	0.0	2.4578886859082485E-4	0.0	1.5341680323310678	3.0723608573853106E-5
50-51	0.0	2.4578886859082485E-4	0.0	1.858762956913826	3.0723608573853106E-5
52-53	0.0	2.4578886859082485E-4	0.0	2.1893489851684853	3.0723608573853106E-5
54-55	0.0	2.9187428145160444E-4	0.0	2.5484618839839674	3.0723608573853106E-5
56-57	0.0	3.0723608573853103E-4	0.0	2.9692831506200332	3.0723608573853106E-5
58-59	0.0	3.0723608573853103E-4	0.0	3.428048073844808	3.0723608573853106E-5
60-61	0.0	3.0723608573853103E-4	0.0	3.9289657880329085	3.0723608573853106E-5
62-63	0.0	3.3795969431238415E-4	0.0	4.4349989830485566	3.0723608573853106E-5
64-65	0.0	3.3795969431238415E-4	0.0	4.966916818287675	3.0723608573853106E-5
66-67	0.0	3.3795969431238415E-4	0.0	5.569836912940968	3.0723608573853106E-5
68-69	0.0	3.8404510717316375E-4	0.0	6.235233465629192	3.0723608573853106E-5
70-71	0.0	3.9940691146009034E-4	0.0	6.945378954205232	3.0723608573853106E-5
72-73	0.0	3.9940691146009034E-4	0.0	7.6742351204027495	3.0723608573853106E-5
74-75	0.0	4.147687157470169E-4	0.0	8.402415367211642	3.0723608573853106E-5
76-77	0.0	4.3013052003394346E-4	0.0	9.182211276424606	3.0723608573853106E-5
78-79	0.0	4.3013052003394346E-4	0.0	10.034007962330397	3.0723608573853106E-5
80-81	0.0	4.3013052003394346E-4	0.0	10.934148246327148	3.0723608573853106E-5
82-83	0.0	4.915777371816497E-4	0.0	11.85365976552971	4.608541286077966E-5
84-85	0.0	4.915777371816497E-4	0.0	12.759099872005446	6.144721714770621E-5
86-87	0.0	4.915777371816497E-4	0.0	13.695724441183646	6.144721714770621E-5
88-89	0.0	4.915777371816497E-4	0.0	14.684271908851656	6.144721714770621E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5080	0.0	41.935074	1
GGTATCA	2995	0.0	41.7563	1
ATCAACG	6475	0.0	32.329533	3
TCAACGC	6540	0.0	32.080795	4
CAACGCA	6590	0.0	31.690403	5
TATCAAC	6775	0.0	31.455568	2
AACGCAG	7005	0.0	29.94939	6
GTACATG	9840	0.0	27.545008	1
TACATGG	10230	0.0	25.98202	2
ACGCAGA	8375	0.0	24.313435	7
ACATGGG	10465	0.0	24.17623	3
CGCAGAG	8625	0.0	23.386414	8
GAGTACA	7495	0.0	22.839888	1
CATGGGG	7025	0.0	21.352139	4
AGTACAT	7440	0.0	21.307575	2
GCAGAGT	9915	0.0	19.43422	9
TACGCTC	345	5.2750693E-11	19.262362	4
GTGTAGC	1885	0.0	18.667383	1
GTACGCT	240	3.113606E-6	17.800488	3
AGAGTAC	8350	0.0	17.618635	10-11
>>END_MODULE