FastQCFastQC Report
Mon 15 Aug 2016
SRR2920900_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920900_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3849430
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64500.039.8888551
GGTATCA37450.037.729931
TCAACGC77000.032.2793354
ATCAACG78600.031.3888763
CAACGCA78800.031.3674035
AACGCAG81800.030.3792696
TATCAAC83600.029.8410632
GTACATG115900.028.0760141
TACATGG120300.026.7223152
ACATGGG121850.025.4035423
ACGCAGA100750.024.1605877
CGCAGAG103900.023.2494968
GAGTACA84800.022.904251
CATGGGG78900.022.376894
AGTACAT85800.020.7379632
GCAGAGT113900.020.0401769
AGAGTAC95500.017.90303810-11
GTATACG2153.1422103E-517.1258051
GTGTAAG14600.016.7079241
CAGAGTA105950.016.69966110-11
Produced by FastQC (version 0.11.5)