##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920900_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3849430
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.813545382043575	31.0	30.0	33.0	23.0	34.0
2	28.8236105085688	31.0	29.0	34.0	22.0	34.0
3	28.912727338852765	31.0	29.0	34.0	22.0	34.0
4	32.41287593228088	35.0	33.0	37.0	25.0	37.0
5	32.393902214094034	35.0	33.0	37.0	25.0	37.0
6	32.52853799133898	35.0	33.0	37.0	25.0	37.0
7	32.37245176558607	35.0	33.0	37.0	24.0	37.0
8	32.323381903294774	35.0	32.0	37.0	23.0	37.0
9	33.6054628867131	37.0	33.0	39.0	23.0	39.0
10-11	33.41639151770522	37.0	33.0	39.0	22.5	39.0
12-13	33.25700207563197	37.0	33.0	39.0	22.0	39.0
14-15	34.05023496985268	38.0	33.0	40.0	19.5	41.0
16-17	33.95803677427567	38.0	33.0	40.0	19.0	41.0
18-19	33.856919465998864	38.0	33.0	40.0	18.0	41.0
20-21	33.76705083609781	38.0	32.0	40.0	18.0	41.0
22-23	33.55777699555519	38.0	32.0	40.0	18.0	41.0
24-25	33.38107888700405	38.0	32.0	40.0	17.5	41.0
26-27	33.42562587188233	38.0	32.0	40.0	17.0	41.0
28-29	33.270993367849265	38.0	32.0	40.0	16.0	41.0
30-31	33.030738317101495	38.0	32.0	40.0	14.5	41.0
32-33	32.780719353254895	38.0	31.0	40.0	9.5	41.0
34-35	32.46073847296873	37.0	31.0	40.0	9.0	41.0
36-37	32.16711993723746	37.0	30.0	40.0	8.0	41.0
38-39	31.96905035290939	37.0	30.0	40.0	8.0	41.0
40-41	31.779462154137107	37.0	30.0	40.0	4.5	41.0
42-43	31.550023509974203	37.0	30.0	40.0	2.0	41.0
44-45	31.27918016433602	36.5	30.0	40.0	2.0	41.0
46-47	31.03783677063877	36.0	29.5	39.5	2.0	41.0
48-49	30.7442251190436	36.0	29.0	39.0	2.0	40.0
50-51	29.58380656356915	34.5	27.0	38.0	2.0	39.5
52-53	30.022062617062787	35.0	28.0	38.5	2.0	39.5
54-55	30.68940843189771	36.0	29.0	39.0	2.0	40.0
56-57	30.655604855783842	36.0	29.0	39.0	2.0	41.0
58-59	30.42936291866588	35.5	28.5	39.0	2.0	41.0
60-61	30.12235278989357	35.0	28.0	39.0	2.0	40.0
62-63	29.741900619052693	35.0	28.0	39.0	2.0	40.0
64-65	29.33703353483503	34.5	27.5	38.0	2.0	40.0
66-67	28.963167144226546	34.0	26.5	38.0	2.0	40.0
68-69	28.522918458057426	34.0	26.0	37.0	2.0	40.0
70-71	28.195854710957207	34.0	26.0	37.0	2.0	39.0
72-73	27.731914335369133	34.0	25.5	36.0	2.0	39.0
74-75	27.281438420753204	33.0	25.0	36.0	2.0	39.0
76-77	26.857875062022167	33.0	24.0	35.0	2.0	37.5
78-79	26.39060523246299	33.0	23.0	35.0	2.0	37.0
80-81	25.931373865741165	32.0	21.0	35.0	2.0	36.5
82-83	25.443392268465722	32.0	19.5	35.0	2.0	36.0
84-85	25.03315399942329	32.0	18.0	35.0	2.0	36.0
86-87	24.567277233252717	31.5	12.5	34.5	2.0	35.0
88-89	24.022034560960975	31.0	4.5	34.0	2.0	35.0
90-91	23.513662412357156	31.0	2.0	34.0	2.0	35.0
92-93	22.961000070140255	31.0	2.0	34.0	2.0	35.0
94-95	22.313849193257184	30.0	2.0	34.0	2.0	35.0
96-97	21.661119308572957	30.0	2.0	34.0	2.0	35.0
98	21.256776457813235	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	174082.0
3	30095.0
4	22484.0
5	18165.0
6	17229.0
7	17482.0
8	20040.0
9	23061.0
10	25390.0
11	27619.0
12	29083.0
13	28968.0
14	27555.0
15	29031.0
16	31837.0
17	34668.0
18	35443.0
19	34942.0
20	35539.0
21	37825.0
22	41726.0
23	46414.0
24	53449.0
25	61303.0
26	70744.0
27	81932.0
28	94251.0
29	109492.0
30	127764.0
31	150598.0
32	178779.0
33	217125.0
34	270353.0
35	345036.0
36	441499.0
37	501929.0
38	320769.0
39	35728.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.011758685739636	17.807060531998047	11.909095722248288	25.272085060014028
2	16.26395933282594	19.915310500879453	38.55141771499977	25.269312451294834
3	19.749506188942686	23.560932464939544	29.123549681731564	27.56601166438621
4	13.296902419645257	15.155611723922547	36.740668259220016	34.80681759721218
5	14.134842496667524	36.90618918069165	34.48256329105701	14.476405031583816
6	33.45595906216521	35.15544876833378	17.142406511306678	14.246185658194335
7	29.156294307975195	31.746835548282814	20.872247270622232	18.224622873119756
8	26.877323816063537	34.16463951833761	20.173925559424088	18.784111106174766
9	26.964121960913694	15.572253554422344	19.381882512475872	38.08174197218809
10-11	25.645943455228082	25.157143497312262	27.93071177467299	21.266201272786667
12-13	26.260088285774373	22.656967173347116	27.56125324076702	23.521691300111492
14-15	23.242234840464846	24.28732720456335	25.08160623410578	27.388831720866026
16-17	23.36603043410149	27.351111369752797	25.84172064152029	23.441137554625428
18-19	23.328240255704635	26.57508049241255	27.347124186938604	22.74955506494421
20-21	23.97923820557031	25.978631403143517	27.126837306234	22.915293085052173
22-23	23.603394592221957	25.83469721342006	26.985180123183465	23.576728071174518
24-25	23.36794780729146	25.97052559887546	27.133709697230042	23.527816896603042
26-27	23.254697280856497	26.20043282823707	27.04560346834529	23.499266422561142
28-29	23.335276308632853	26.387800974346874	26.96153103854304	23.315391678477226
30-31	23.551774719024536	26.203570218556614	26.74564385848929	23.499011203929555
32-33	23.2190891602911	26.27166900495464	26.783931408025254	23.725310426729003
34-35	23.66583075998425	26.20118408599476	26.766766915509553	23.366218238511433
36-37	23.6739165921215	25.9298208957173	26.795724481067708	23.600538031093496
38-39	23.463341583909642	26.145451094810102	26.82891364279325	23.562293678487013
40-41	23.65639416336622	25.93222706374713	26.976886867573995	23.43449190531265
42-43	23.432717677756017	25.911509518865966	26.843910481679394	23.81186232169862
44-45	23.318283582284234	25.940355089601972	26.94378921896253	23.79757210915126
46-47	23.44102374806469	25.89289045003541	26.810504608874968	23.85558119302493
48-49	23.212246968710915	26.044906247513865	26.80835940648423	23.934487377290992
50-51	23.152506090746748	26.146554302099734	26.817713447401132	23.88322615975239
52-53	23.30091512875066	25.94998371434119	26.857074030846707	23.89202712606144
54-55	22.97514912742026	25.772402390214683	27.127240173772105	24.12520830859295
56-57	22.879035315426922	25.933307954770495	27.05364800316023	24.13400872664235
58-59	22.87685155981172	25.702059409050488	27.10298245298234	24.31810657815546
60-61	22.821750041355543	25.677239422243492	27.248019499986693	24.252991036414272
62-63	22.79572694655266	25.64763352183352	27.268928147152227	24.287711384461595
64-65	22.721699416923546	25.54523951390199	27.116187007268294	24.61687406190617
66-67	22.670670645584824	25.594713432138	27.060924671655062	24.673691250622117
68-69	22.87853040167485	25.349963220124717	27.03408885897856	24.73741751922187
70-71	22.739534227520387	25.261231950292846	27.056416817420452	24.942817004766315
72-73	22.849933249199513	25.2336278017084	27.009811846181126	24.90662710291096
74-75	22.728466016866168	25.31879555060112	27.02470434146957	24.92803409106314
76-77	22.806455678894405	25.119844459312713	27.172017483728432	24.901682378064447
78-79	22.862374596805413	25.2065406875574	27.01639004079066	24.914694674846523
80-81	22.966905893319254	25.14255170750468	26.97857389626559	24.91196850291048
82-83	22.99036063502509	25.00342526425376	27.091200414655773	24.915013686065375
84-85	22.967144018484326	24.909007145415966	27.066789276713703	25.057059559386
86-87	23.008072921147203	24.863296310720916	27.125906600410737	25.00272416772114
88-89	23.168932794407525	24.657616239130334	27.051824925266555	25.12162604119559
90-91	23.221418849505373	24.591705299826348	26.99464437502933	25.19223147563895
92-93	23.50860900580104	24.321987753954364	27.060564492359813	25.10883874788479
94-95	23.461511686026697	24.123688142826637	27.16367946555517	25.251120705591497
96-97	23.663538072743556	23.895239578940235	27.120600679603868	25.320621668712345
98	23.75770798423453	23.845910971559945	27.04512692183046	25.35125412237506
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	148.0
1	119.0
2	191.5
3	330.0
4	304.5
5	332.5
6	553.5
7	942.0
8	1405.5
9	1640.0
10	1590.0
11	1601.5
12	1788.5
13	1969.5
14	2142.5
15	2340.0
16	2517.0
17	2610.0
18	2694.0
19	2803.0
20	2863.0
21	3048.0
22	3407.5
23	3886.0
24	4640.0
25	5832.0
26	7592.5
27	9842.5
28	12750.5
29	17175.5
30	23645.0
31	32216.0
32	40657.5
33	48404.0
34	58308.0
35	69997.5
36	81567.0
37	92510.0
38	102156.0
39	110296.5
40	117904.5
41	124961.0
42	133890.0
43	144176.0
44	151662.5
45	158007.5
46	163517.5
47	166672.5
48	169135.0
49	169516.0
50	168542.0
51	167452.5
52	164480.5
53	160669.0
54	154438.0
55	145056.5
56	132191.5
57	118606.0
58	106688.0
59	91702.5
60	75897.0
61	61918.0
62	49806.0
63	38604.0
64	28540.5
65	21754.0
66	16857.5
67	13188.0
68	10384.5
69	8258.0
70	6278.5
71	4568.0
72	3542.5
73	2904.0
74	2308.0
75	1780.0
76	1286.5
77	820.5
78	495.5
79	289.0
80	172.5
81	107.5
82	62.5
83	34.5
84	21.5
85	17.0
86	12.0
87	9.0
88	6.0
89	6.0
90	6.5
91	4.5
92	3.5
93	3.0
94	3.5
95	4.5
96	3.0
97	1.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3959807036366423
2	0.026471451617512205
3	0.020574474662482498
4	0.017768864481234884
5	0.02441919972567367
6	0.022418903577932317
7	0.01711941768002016
8	0.011092551364747509
9	0.0
10-11	6.494468012147253E-5
12-13	0.005858010146956822
14-15	0.003974614423434119
16-17	0.006780224604681732
18-19	0.03627809831585456
20-21	0.07892077528361341
22-23	0.09830026783186083
24-25	0.06578896096305167
26-27	0.07454350384342616
28-29	0.058125488708717914
30-31	0.0538910955647979
32-33	0.021210932527672928
34-35	0.35006481479076124
36-37	0.536572947163606
38-39	0.4880852489849146
40-41	0.49721647100999367
42-43	0.4447281805358196
44-45	0.419386766352421
46-47	0.4173345144605825
48-49	0.40694336564114686
50-51	0.40370912057109754
52-53	0.46339328160273074
54-55	0.46695225007338753
56-57	0.4364022725442468
58-59	0.4219585756852313
60-61	0.43673998488087845
62-63	0.41595768724200727
64-65	0.463886861171654
66-67	0.4868383111265823
68-69	0.42841407688930566
70-71	0.3847582629116518
72-73	0.37070423413336523
74-75	0.3529353696521303
76-77	0.3512338190329477
78-79	0.3507792062720974
80-81	0.3677167788477775
82-83	0.3626381048622783
84-85	0.36275500528649696
86-87	0.3479995739628984
88-89	0.3932010713274433
90-91	0.3691455618104499
92-93	0.3591310921357188
94-95	0.3668854869422226
96-97	0.3664828299254695
98	0.362625115926254
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3849430.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.39441823495004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.76545102454197	46.1883372238117
2	12.041401185925178	14.303840363433268
3	4.366989210663749	7.781243508170312
4	1.94994722509952	4.632639240945647
5	1.0868351634935163	3.2275971126492102
6	0.6738227398890487	2.4012785777514067
7	0.4124181741592861	1.7146736266597775
8	0.31858563228579995	1.5137766630103042
9	0.2214075590638573	1.183533584707633
>10	1.0752160010740475	11.278011741568994
>50	0.062090459482748224	2.506939221672617
>100	0.024691509375756638	2.538616387181872
>500	6.600663460439007E-4	0.24808453114085788
>1k	4.8404859947755006E-4	0.48142821729639856
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.5977872048589012E-5	0.0
4	0.0	0.0	0.0	2.5977872048589012E-5	0.0
5	0.0	0.0	0.0	2.5977872048589012E-5	0.0
6	0.0	0.0	0.0	2.5977872048589012E-5	0.0
7	0.0	0.0	0.0	2.5977872048589012E-5	0.0
8	0.0	0.0	0.0	2.5977872048589012E-5	0.0
9	0.0	0.0	0.0	2.5977872048589012E-5	2.5977872048589012E-5
10-11	0.0	0.0	0.0	2.5977872048589012E-5	2.5977872048589012E-5
12-13	0.0	2.5977872048589012E-5	0.0	5.1955744097178025E-5	5.1955744097178025E-5
14-15	0.0	6.494468012147253E-5	0.0	5.1955744097178025E-5	1.1690042421865055E-4
16-17	0.0	7.793361614576703E-5	0.0	9.092255217006155E-5	1.8184510434012308E-4
18-19	0.0	7.793361614576703E-5	0.0	1.4287829626723957E-4	2.078229763887121E-4
20-21	0.0	7.793361614576703E-5	0.0	2.7276765651018463E-4	2.4678978446159564E-4
22-23	0.0	7.793361614576703E-5	0.0	6.624357372390198E-4	3.117344645830681E-4
24-25	0.0	7.793361614576703E-5	0.0	0.0013248714744780396	3.6369020868024616E-4
26-27	0.0	7.793361614576703E-5	0.0	0.0026757208210046683	3.8966808072883515E-4
28-29	0.0	7.793361614576703E-5	0.0	0.012183621990788247	4.0265701675312965E-4
30-31	0.0	7.793361614576703E-5	0.0	0.05238437898597974	4.156459527774242E-4
32-33	0.0	7.793361614576703E-5	0.0	0.1345523882756668	4.416238248260132E-4
34-35	0.0	1.0391148819435605E-4	0.0	0.24569871383555486	4.416238248260132E-4
36-37	0.0	1.4287829626723957E-4	0.0	0.41604860979417735	4.416238248260132E-4
38-39	0.0	1.8184510434012308E-4	0.0	0.6985969351306557	4.416238248260132E-4
40-41	1.2988936024294506E-5	1.948340403644176E-4	0.0	1.0191378983381956	4.416238248260132E-4
42-43	2.5977872048589012E-5	2.5977872048589014E-4	0.0	1.3628511234130767	4.416238248260132E-4
44-45	2.5977872048589012E-5	2.5977872048589014E-4	0.0	1.7581693913124798	4.8059063289889673E-4
46-47	2.5977872048589012E-5	2.5977872048589014E-4	0.0	2.225680165634912	5.195574409717803E-4
48-49	2.5977872048589012E-5	2.5977872048589014E-4	0.0	2.7177530179792853	5.715131850689583E-4
50-51	2.5977872048589012E-5	2.5977872048589014E-4	0.0	3.2593396944482693	5.715131850689583E-4
52-53	2.5977872048589012E-5	2.5977872048589014E-4	0.0	3.805784232990339	7.143914813361979E-4
54-55	2.5977872048589012E-5	2.8575659253447913E-4	0.0	4.409977581096422	8.053140335062594E-4
56-57	2.5977872048589012E-5	2.8575659253447913E-4	0.0	5.094715321489156	8.053140335062594E-4
58-59	2.5977872048589012E-5	2.8575659253447913E-4	0.0	5.821160535455899	8.053140335062594E-4
60-61	2.5977872048589012E-5	2.8575659253447913E-4	0.0	6.58806628513832	8.572697776034374E-4
62-63	2.5977872048589012E-5	2.8575659253447913E-4	0.0	7.367207612555625	8.572697776034374E-4
64-65	2.5977872048589012E-5	2.8575659253447913E-4	0.0	8.187030287601022	8.572697776034374E-4
66-67	2.5977872048589012E-5	3.3771233663165717E-4	0.0	9.107439283218554	8.572697776034374E-4
68-69	2.5977872048589012E-5	3.3771233663165717E-4	0.0	10.10292432905651	8.572697776034374E-4
70-71	2.5977872048589012E-5	3.5070127265595167E-4	0.0	11.143584894386962	8.832476496520264E-4
72-73	2.5977872048589012E-5	3.6369020868024616E-4	0.0	12.181998373785209	9.741702018220879E-4
74-75	2.5977872048589012E-5	3.6369020868024616E-4	0.0	13.23051724541036	0.001000148073870677
76-77	2.5977872048589012E-5	4.416238248260132E-4	0.0	14.364308482035003	0.0010391148819435606
78-79	2.5977872048589012E-5	4.416238248260132E-4	0.0	15.576423002886141	0.0010650927539921494
80-81	2.5977872048589012E-5	4.416238248260132E-4	0.0	16.815593477475886	0.0010910706260407383
82-83	2.5977872048589012E-5	4.416238248260132E-4	0.0	18.06166107709453	0.0011430263701379165
84-85	2.5977872048589012E-5	4.416238248260132E-4	0.0	19.29385649303923	0.0011430263701379165
86	2.5977872048589012E-5	4.416238248260132E-4	0.0	20.243490594711425	0.0011430263701379165
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6450	0.0	39.888855	1
GGTATCA	3745	0.0	37.72993	1
TCAACGC	7700	0.0	32.279335	4
ATCAACG	7860	0.0	31.388876	3
CAACGCA	7880	0.0	31.367403	5
AACGCAG	8180	0.0	30.379269	6
TATCAAC	8360	0.0	29.841063	2
GTACATG	11590	0.0	28.076014	1
TACATGG	12030	0.0	26.722315	2
ACATGGG	12185	0.0	25.403542	3
ACGCAGA	10075	0.0	24.160587	7
CGCAGAG	10390	0.0	23.249496	8
GAGTACA	8480	0.0	22.90425	1
CATGGGG	7890	0.0	22.37689	4
AGTACAT	8580	0.0	20.737963	2
GCAGAGT	11390	0.0	20.040176	9
AGAGTAC	9550	0.0	17.903038	10-11
GTATACG	215	3.1422103E-5	17.125805	1
GTGTAAG	1460	0.0	16.707924	1
CAGAGTA	10595	0.0	16.699661	10-11
>>END_MODULE