FastQCFastQC Report
Mon 15 Aug 2016
SRR2920900_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920900_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3849430
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63250.039.9299551
GGTATCA35150.038.022951
TCAACGC75350.033.011014
CAACGCA76650.032.5075655
ATCAACG78200.031.7472173
AACGCAG80600.030.9144546
TATCAAC82000.030.2169382
GTACATG110550.028.266461
TACATGG115000.026.416542
ACATGGG114900.025.8621773
ACGCAGA98150.024.9515047
CGCAGAG103800.023.4546598
CATGGGG76450.022.8497314
GAGTACA82000.022.67921
AGTACAT81500.020.7341482
GCAGAGT117900.019.965359
AGAGTAC94100.018.83570110-11
GTATAGG10600.017.9481181
GAGTACT52050.017.2296612-13
ATGGGGG39800.016.6948415
Produced by FastQC (version 0.11.5)