##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920900_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3849430
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.247379482157097	31.0	30.0	34.0	27.0	34.0
2	30.258080546990072	31.0	30.0	34.0	26.0	34.0
3	30.40706182473769	31.0	30.0	34.0	27.0	34.0
4	34.04596109034325	35.0	35.0	37.0	30.0	37.0
5	33.56718033578998	35.0	35.0	37.0	28.0	37.0
6	33.50760242425502	35.0	35.0	37.0	28.0	37.0
7	33.31569712918536	35.0	33.0	37.0	27.0	37.0
8	33.2566808592441	35.0	33.0	37.0	27.0	37.0
9	34.58742437192	38.0	34.0	39.0	27.0	39.0
10-11	34.4020226371177	37.5	34.0	39.0	27.0	39.0
12-13	34.22419228301332	37.0	34.0	39.0	26.0	39.0
14-15	35.12835328866871	38.0	34.0	40.0	26.0	41.0
16-17	35.01591703187225	38.0	34.0	40.0	25.5	41.0
18-19	34.93624432708219	38.5	34.0	40.0	25.0	41.0
20-21	34.82702633896447	38.0	34.0	40.0	25.0	41.0
22-23	34.68332922017026	38.0	34.0	40.0	24.5	41.0
24-25	34.514131702615714	38.0	33.0	40.0	24.0	41.0
26-27	34.34015620494463	38.0	33.0	40.0	23.5	41.0
28-29	34.27851824815622	38.0	33.0	40.0	23.5	41.0
30-31	34.26382970985315	38.0	33.0	40.0	23.0	41.0
32-33	34.11292282233993	38.0	33.0	40.0	22.0	41.0
34-35	33.94793670751254	38.0	33.0	40.0	21.5	41.0
36-37	33.75889962929577	38.0	33.0	40.0	19.5	41.0
38-39	33.57404057224056	38.0	32.5	40.0	18.5	41.0
40-41	33.34621801149781	38.0	32.0	40.0	17.0	41.0
42-43	33.37218666140182	38.0	32.0	40.0	17.0	41.0
44-45	33.22911547943461	38.0	32.0	40.0	15.0	41.0
46-47	32.99215091584988	38.0	32.0	40.0	14.5	41.0
48-49	32.7363217411409	37.0	31.0	40.0	10.5	41.0
50-51	32.49944511265304	37.0	31.0	40.0	9.0	41.0
52-53	32.25414178203007	37.0	31.0	40.0	8.0	41.0
54-55	31.986969109712348	36.5	31.0	40.0	7.5	41.0
56-57	31.68026629916637	36.0	30.0	39.5	4.0	41.0
58-59	31.368411037478275	36.0	30.0	39.0	2.0	41.0
60-61	31.059994596602614	35.0	29.0	39.0	2.0	40.0
62-63	30.754025531052648	35.0	29.0	39.0	2.0	40.0
64-65	30.372049368348044	35.0	29.0	38.5	2.0	40.0
66-67	29.96674975256077	34.5	28.0	38.0	2.0	40.0
68-69	29.539608981069925	34.0	28.0	37.5	2.0	40.0
70-71	29.128225997095676	34.0	27.5	37.0	2.0	39.0
72-73	28.699526423392555	34.0	26.5	36.0	2.0	39.0
74-75	28.26482427268453	34.0	26.0	36.0	2.0	39.0
76-77	26.764160538053687	31.5	25.0	34.5	2.0	36.5
78-79	27.61444681420366	33.0	26.0	35.0	2.0	37.0
80-81	27.643056374580134	33.0	26.0	35.0	2.0	37.0
82-83	27.31487726754351	33.0	26.0	35.0	2.0	36.0
84-85	27.002579342915705	33.0	25.5	35.0	2.0	36.0
86-87	26.482647950475783	33.0	25.0	35.0	2.0	35.5
88-89	26.124674692097273	32.5	24.0	35.0	2.0	35.0
90-91	25.776409884060755	32.0	23.0	35.0	2.0	35.0
92-93	25.483225958128866	32.0	20.5	35.0	2.0	35.0
94-95	25.121400700882987	32.0	19.5	34.5	2.0	35.0
96-97	24.627558755452107	32.0	15.0	34.0	2.0	35.0
98-99	24.072042614101306	32.0	2.0	34.0	2.0	35.0
100-101	21.898547187505685	28.5	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91814.0
3	41127.0
4	21957.0
5	15059.0
6	13045.0
7	12962.0
8	14460.0
9	16924.0
10	19033.0
11	21162.0
12	24039.0
13	26038.0
14	28079.0
15	30357.0
16	32184.0
17	33741.0
18	32075.0
19	30256.0
20	30665.0
21	31649.0
22	34166.0
23	39001.0
24	45604.0
25	53663.0
26	64225.0
27	76575.0
28	90558.0
29	106947.0
30	126007.0
31	149949.0
32	179513.0
33	219240.0
34	276760.0
35	359649.0
36	484264.0
37	585476.0
38	362435.0
39	28770.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.23745525520508	18.959351828065465	12.521280862619003	25.281912054110457
2	16.240326489895907	20.84157914288609	37.50066893020525	25.417425437012753
3	19.513203313728027	24.19766596569773	29.374937320698223	26.91419339987602
4	13.666299114236748	15.955767957672926	36.3105772998095	34.06735562828082
5	14.125134087453207	37.118628524041696	34.45807885186587	14.298158536639228
6	33.528522983782885	35.54315375573424	16.881455899639043	14.046867360843832
7	29.056445522400292	31.845591236610932	20.917916460387506	18.18004678060127
8	26.875408036664126	34.41730413589864	20.07444149371981	18.632846333717417
9	26.809005484042164	15.456413831857393	19.406979406224963	38.32760127787548
10-11	25.635863053323423	25.30479423641947	27.902109014606353	21.15723369565076
12-13	26.211863970375965	22.703255616535404	27.6538588176914	23.431021595397226
14-15	23.172119665490914	24.340909220950877	25.101601646641097	27.38536946691711
16-17	23.358263069655045	27.432839378732655	25.892382616191284	23.31651493542102
18-19	23.324875142017017	26.65558695601307	27.358717440079865	22.66082046189004
20-21	23.946325154593897	25.996424102484806	27.0775613730303	22.979689369890995
22-23	23.629681946827212	25.85971621574717	27.008747472990525	23.501854364435097
24-25	23.3604725091404	26.011552279758348	27.124099931222815	23.503875279878436
26-27	23.264927604044814	26.24232498895214	27.05348462398295	23.439262783020094
28-29	23.32914184835002	26.423650630522438	26.95519000655036	23.292017514577182
30-31	23.523628684034275	26.199188114168116	26.7265046489526	23.55067855284501
32-33	23.23583807127876	26.281623008445276	26.77367290103106	23.708866019244905
34-35	23.63980118349837	26.216116743569767	26.80942490719857	23.334657165733294
36-37	23.697649177611023	25.947912463170553	26.774705773677574	23.579732585540846
38-39	23.479032552507135	26.153604280478955	26.85929081829208	23.508072348721825
40-41	23.657097243504115	25.948421620731132	26.972412174906772	23.42206896085798
42-43	23.4007749204905	25.947066585847267	26.82617239006046	23.825986103601775
44-45	23.31638860262229	25.92107253051533	26.949956267471574	23.81258259939081
46-47	23.357706627825316	25.92309386436148	26.84839984070195	23.870799667111253
48-49	23.161948621608417	26.04600930172864	26.840746835046758	23.951295241616187
50-51	23.065239123077895	26.15995494702573	26.850832174423083	23.923973755473295
52-53	23.2740834633512	25.93891821065453	26.801783926089666	23.985214399904603
54-55	22.96940527792829	25.76350403120048	26.989914431740576	24.277176259130655
56-57	22.767259504310864	25.93870145876042	26.907207602981064	24.38683143394765
58-59	22.775679692576034	25.693760152880657	26.93479840499917	24.595761749544135
60-61	22.701907030555233	25.742931215810742	27.05099164679432	24.504170106839705
62-63	22.635549554008403	25.678398911927296	26.99146468277861	24.69458685128569
64-65	22.44734657060345	25.60798122797555	26.849416959459948	25.095255241961045
66-67	22.434897635257833	25.696782581068938	26.709989195356147	25.158330588317078
68-69	22.613637353455506	25.49527447342006	26.71155950502519	25.17952866809925
70-71	22.37784351039914	25.33422647474866	26.694010894385162	25.59391912046704
72-73	22.42141808377194	25.331062898149696	26.561986506036494	25.68553251204187
74-75	22.10122257341414	25.44839803661285	26.523211708666043	25.927167681306962
76-77	22.122178447373514	25.237114728446763	26.73612621324582	25.90458061093391
78-79	22.04482321073939	25.23727744291672	26.59914876380178	26.118750582542106
80-81	21.98385855285593	25.23130986057297	26.543281488001092	26.241550098570006
82-83	21.87594952659259	25.14561410459845	26.596266651424443	26.382169717384514
84-85	21.734816970687092	24.970218910816257	26.56393912160791	26.731024996888735
86-87	21.610151611526916	24.945039974952817	26.633042071247985	26.811766342272282
88-89	21.657457191461884	24.77621792178035	26.532647290061544	27.033677596696222
90-91	21.48261011751538	24.72620965068898	26.481445135330034	27.309735096465605
92-93	21.565034058581595	24.515374331550802	26.552814786388247	27.366776823479356
94-95	21.30385954958543	24.246834625876794	26.70058895091608	27.7487168736217
96-97	21.2772587612135	24.050150327322058	26.78886655563254	27.8837243558319
98-99	21.16150239303312	24.086983110431277	26.686625279044314	28.06488921749129
100-101	21.231773665333243	23.86376212642606	26.736889223269642	28.16757498497105
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	141.0
1	109.5
2	138.0
3	214.0
4	193.5
5	230.5
6	410.0
7	706.5
8	1027.0
9	1119.5
10	956.5
11	795.5
12	793.0
13	876.5
14	926.0
15	965.5
16	1051.0
17	1185.0
18	1372.5
19	1542.0
20	1725.5
21	2011.5
22	2388.5
23	2935.0
24	3626.5
25	4487.5
26	5911.0
27	7884.0
28	10102.5
29	13523.5
30	18755.5
31	25597.0
32	34328.0
33	42022.5
34	49378.5
35	58735.5
36	70565.0
37	82098.5
38	91657.0
39	99971.0
40	107825.0
41	115581.0
42	123249.5
43	132983.5
44	141947.0
45	150096.5
46	157043.5
47	161737.0
48	165519.5
49	167961.5
50	167910.5
51	166579.5
52	164442.0
53	162066.0
54	157287.5
55	147228.5
56	134950.0
57	121138.0
58	108145.5
59	94095.0
60	78078.0
61	63714.0
62	51016.0
63	39366.5
64	29425.0
65	22328.5
66	17134.5
67	13380.5
68	10630.0
69	8334.0
70	6470.5
71	4859.0
72	3574.5
73	3002.0
74	2510.5
75	1931.0
76	1391.5
77	890.5
78	547.5
79	298.0
80	168.5
81	98.0
82	55.5
83	26.5
84	11.0
85	4.5
86	4.0
87	4.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19127507189376092
2	0.0
3	0.012261555606934014
4	0.0141839181385296
5	0.0068321803487789105
6	0.10676905411970085
7	0.14363165455664864
8	0.12469378583322725
9	0.14417718986966901
10-11	0.1158353314646584
12-13	0.11544566338392956
14-15	0.11721215868323362
16-17	0.06628254053197485
18-19	0.07949228846868238
20-21	0.03753802511021113
22-23	0.08399944926911257
24-25	0.05777478743606196
26-27	0.0657240162829302
28-29	0.040252712739288675
30-31	0.025523259287738704
32-33	0.03229049495639614
34-35	0.019626282332708998
36-37	0.024601044830013796
38-39	0.032797063461343624
40-41	0.024860823550499685
42-43	0.013482515593217696
44-45	0.02691307544233822
46-47	0.033706288983044244
48-49	0.00936502287351634
50-51	0.016301114710489603
52-53	0.007260815237580629
54-55	0.013365615168999046
56-57	0.005273508025863569
58-59	0.003974614423434119
60-61	0.02357491888409453
62-63	0.010975650940528858
64-65	0.007741405870479526
66-67	0.040200756995191496
68-69	0.08979251473594792
70-71	0.021873368264911947
72-73	0.04440917226706292
74-75	0.0862855020093884
76-77	0.25544041585377575
78-79	0.08160948504064239
80-81	0.016690782791218443
82-83	0.012274544542958309
84-85	0.013274692616828985
86-87	0.2669486131713007
88-89	0.6263914397716026
90-91	0.2679747391172199
92-93	0.3555331568569892
94-95	0.0011170484980893274
96-97	0.004234393143920009
98-99	0.0034031012383651605
100-101	0.00498775143332909
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3849430.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.217770687516555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06557805255767	43.52302802224637
2	12.884752593778659	14.744736385524233
3	4.636715685110314	7.959076044415596
4	2.1875779814480185	5.006733412142124
5	1.1504115648542554	3.2911992557048935
6	0.7120418257622022	2.4444867543829383
7	0.4758186079761624	1.9057696000023376
8	0.35110400870920255	1.6071510926232775
9	0.24345066376173988	1.2536733827558635
>10	1.1926905153244272	12.0719456320041
>50	0.07047680981850654	2.7329074810762353
>100	0.028104506507926202	2.7180768481447273
>500	7.298196689445714E-4	0.26788176621695536
>1k	5.473647221576135E-4	0.47333432276030657
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.5977872048589012E-5	0.0
5	0.0	0.0	0.0	2.5977872048589012E-5	0.0
6	0.0	0.0	0.0	2.5977872048589012E-5	2.5977872048589012E-5
7	0.0	0.0	0.0	2.5977872048589012E-5	2.5977872048589012E-5
8	0.0	0.0	0.0	2.5977872048589012E-5	7.793361614576703E-5
9	0.0	0.0	0.0	2.5977872048589012E-5	7.793361614576703E-5
10-11	0.0	0.0	0.0	5.1955744097178025E-5	7.793361614576703E-5
12-13	0.0	0.0	0.0	7.793361614576703E-5	1.948340403644176E-4
14-15	0.0	2.5977872048589012E-5	0.0	7.793361614576703E-5	2.5977872048589014E-4
16-17	0.0	5.1955744097178025E-5	0.0	1.0391148819435605E-4	3.117344645830681E-4
18-19	0.0	5.1955744097178025E-5	0.0	1.948340403644176E-4	3.117344645830681E-4
20-21	0.0	5.1955744097178025E-5	0.0	3.896680807288352E-4	3.7667914470454066E-4
22-23	0.0	7.793361614576703E-5	0.0	8.572697776034374E-4	4.8059063289889673E-4
24-25	0.0	7.793361614576703E-5	0.0	0.0015326944508667517	5.974910571175473E-4
26-27	0.0	7.793361614576703E-5	0.0	0.002831588053296202	5.974910571175473E-4
28-29	0.0	7.793361614576703E-5	0.0	0.01219661092681254	5.974910571175473E-4
30-31	0.0	7.793361614576703E-5	0.0	0.05304681472321876	6.104799931418417E-4
32-33	0.0	1.0391148819435605E-4	0.0	0.13557851422158607	6.494468012147253E-4
34-35	0.0	1.0391148819435605E-4	0.0	0.24742624232678606	7.533582894090813E-4
36-37	0.0	1.1690042421865056E-4	0.0	0.420620715274729	7.533582894090813E-4
38-39	0.0	1.2988936024294507E-4	0.0	0.7057018831359448	7.793361614576703E-4
40-41	2.5977872048589012E-5	1.2988936024294507E-4	0.0	1.03221775691466	8.053140335062594E-4
42-43	2.5977872048589012E-5	1.2988936024294507E-4	0.0	1.3818799146886682	8.053140335062594E-4
44-45	2.5977872048589012E-5	1.2988936024294507E-4	0.0	1.782900325502737	8.053140335062594E-4
46-47	2.5977872048589012E-5	1.2988936024294507E-4	0.0	2.2563730214603197	8.572697776034374E-4
48-49	2.5977872048589012E-5	1.2988936024294507E-4	0.0	2.7510176831375035	8.832476496520264E-4
50-51	3.8966808072883515E-5	1.2988936024294507E-4	0.0	3.291318454940082	8.962365856763209E-4
52-53	5.1955744097178025E-5	1.2988936024294507E-4	0.0	3.835243139893439	9.481923297734989E-4
54-55	5.1955744097178025E-5	1.4287829626723957E-4	0.0	4.435487851448136	0.0010131370098949715
56-57	5.1955744097178025E-5	1.5586723229153406E-4	0.0	5.116900424218651	0.0010391148819435606
58-59	5.1955744097178025E-5	1.5586723229153406E-4	0.0	5.841319364165604	0.0010521038179678549
60-61	5.1955744097178025E-5	1.5586723229153406E-4	0.0	6.607341866198372	0.0010650927539921494
62-63	5.1955744097178025E-5	1.5586723229153406E-4	0.0	7.383924373218893	0.0011430263701379165
64-65	5.1955744097178025E-5	1.5586723229153406E-4	0.0	8.201486973396062	0.001207971050259389
66-67	5.1955744097178025E-5	2.3380084843730112E-4	0.0	9.117492719701358	0.0012469378583322725
68-69	5.1955744097178025E-5	2.4678978446159564E-4	0.0	10.106457319655117	0.0012729157303808616
70-71	5.1955744097178025E-5	2.7276765651018463E-4	0.0	11.14213013355224	0.0013248714744780396
72-73	5.1955744097178025E-5	2.8575659253447913E-4	0.0	12.17662095427115	0.0013638382825509232
74-75	5.1955744097178025E-5	2.8575659253447913E-4	0.0	13.228568905006716	0.0013898161545995121
76-77	5.1955744097178025E-5	2.8575659253447913E-4	0.0	14.367828483697586	0.0014157940266481012
78-79	5.1955744097178025E-5	2.8575659253447913E-4	0.0	15.581852378144296	0.0014287829626723958
80-81	5.1955744097178025E-5	2.8575659253447913E-4	0.0	16.823789496107217	0.0014677497707452792
82-83	5.1955744097178025E-5	2.8575659253447913E-4	0.0	18.073493997812662	0.0015586723229153406
84-85	5.1955744097178025E-5	2.8575659253447913E-4	2.5977872048589012E-5	19.312651483466382	0.0015716612589396352
86-87	6.494468012147253E-5	2.8575659253447913E-4	2.5977872048589012E-5	20.60076426899567	0.0015846501949639297
88-89	7.793361614576703E-5	2.8575659253447913E-4	2.5977872048589012E-5	21.947613023226815	0.0015976391309882243
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6325	0.0	39.929955	1
GGTATCA	3515	0.0	38.02295	1
TCAACGC	7535	0.0	33.01101	4
CAACGCA	7665	0.0	32.507565	5
ATCAACG	7820	0.0	31.747217	3
AACGCAG	8060	0.0	30.914454	6
TATCAAC	8200	0.0	30.216938	2
GTACATG	11055	0.0	28.26646	1
TACATGG	11500	0.0	26.41654	2
ACATGGG	11490	0.0	25.862177	3
ACGCAGA	9815	0.0	24.951504	7
CGCAGAG	10380	0.0	23.454659	8
CATGGGG	7645	0.0	22.849731	4
GAGTACA	8200	0.0	22.6792	1
AGTACAT	8150	0.0	20.734148	2
GCAGAGT	11790	0.0	19.96535	9
AGAGTAC	9410	0.0	18.835701	10-11
GTATAGG	1060	0.0	17.948118	1
GAGTACT	5205	0.0	17.22966	12-13
ATGGGGG	3980	0.0	16.694841	5
>>END_MODULE