FastQCFastQC Report
Mon 15 Aug 2016
SRR2920899_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920899_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052951
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.044.6164251
GTATCAA49800.044.265821
ATCAACG59250.036.223233
TCAACGC59300.036.115354
CAACGCA61700.034.859195
TATCAAC64850.033.661522
AACGCAG64900.033.276826
GTACATG97550.026.9855381
ACGCAGA78700.026.855167
CGCAGAG79200.026.2212418
TACATGG100150.025.5059192
ACATGGG102450.024.7538533
GCAGAGT87500.022.7908979
CATGGGG69900.021.5848164
GAGTACA73250.021.4872471
GTACTAG4850.020.8758071
GTATAGG6200.020.0416931
AGTACAT74200.019.8398272
AGAGTAC77350.018.52153810-11
GTGTAGC21700.018.2390021
Produced by FastQC (version 0.11.5)