##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920899_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3052951
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.717122547987177	31.0	30.0	33.0	22.0	34.0
2	28.7629175836756	31.0	29.0	34.0	20.0	34.0
3	28.842721681415785	31.0	29.0	34.0	22.0	34.0
4	32.323625567524665	35.0	33.0	37.0	24.0	37.0
5	32.296648062808735	35.0	33.0	37.0	24.0	37.0
6	32.41401810903614	35.0	33.0	37.0	25.0	37.0
7	32.250155013952075	35.0	32.0	37.0	23.0	37.0
8	32.18508190927401	35.0	32.0	37.0	23.0	37.0
9	33.46321837461525	37.0	33.0	39.0	22.0	39.0
10-11	33.27639028598887	37.0	33.0	39.0	21.0	39.0
12-13	33.11033144652502	37.0	32.5	39.0	19.0	39.0
14-15	33.90563065047556	38.0	33.0	40.0	18.0	41.0
16-17	33.805518332917885	38.0	33.0	40.0	17.5	41.0
18-19	33.68872985514671	38.0	32.0	40.0	17.5	41.0
20-21	33.593369824802295	38.0	32.0	40.0	18.0	41.0
22-23	33.380261589524366	38.0	32.0	40.0	16.5	41.0
24-25	33.19432067530727	38.0	32.0	40.0	16.0	41.0
26-27	33.23799104538527	38.0	32.0	40.0	15.0	41.0
28-29	33.086162535854655	38.0	32.0	40.0	10.5	41.0
30-31	32.83864087566423	38.0	31.5	40.0	9.5	41.0
32-33	32.59830668752954	38.0	31.0	40.0	9.0	41.0
34-35	32.2744785291346	37.5	30.5	40.0	8.0	41.0
36-37	31.978719278494808	37.0	30.0	40.0	6.0	41.0
38-39	31.782603291045287	37.0	30.0	40.0	2.0	41.0
40-41	31.58488017003876	37.0	30.0	40.0	2.0	41.0
42-43	31.353084278129586	37.0	30.0	40.0	2.0	41.0
44-45	31.08104912263577	36.5	29.5	40.0	2.0	41.0
46-47	30.832103921746533	36.0	29.0	39.5	2.0	41.0
48-49	30.533111078428707	36.0	28.0	39.0	2.0	40.5
50-51	29.387141490315436	34.5	26.5	38.0	2.0	39.5
52-53	29.823142264648205	35.0	28.0	38.5	2.0	39.5
54-55	30.47197825972313	36.0	28.5	39.0	2.0	40.0
56-57	30.425232995878414	36.0	28.0	39.0	2.0	41.0
58-59	30.193927940540153	35.5	28.0	39.0	2.0	41.0
60-61	29.874948697178567	35.0	28.0	39.0	2.0	40.0
62-63	29.486588058570216	35.0	27.5	39.0	2.0	40.0
64-65	29.07415480955967	34.5	26.5	38.0	2.0	40.0
66-67	28.698474525139773	34.0	26.0	38.0	2.0	40.0
68-69	28.25681987689943	34.0	26.0	37.0	2.0	40.0
70-71	27.927898613505427	34.0	26.0	37.0	2.0	39.0
72-73	27.470625306465777	33.5	25.0	36.0	2.0	39.0
74-75	27.035486157491555	33.0	24.0	36.0	2.0	39.0
76-77	26.621909260908545	33.0	23.0	35.0	2.0	37.5
78-79	26.176407678996483	33.0	21.5	35.0	2.0	37.0
80-81	25.741918229280458	32.0	20.0	35.0	2.0	36.5
82-83	25.280875945929036	32.0	18.5	35.0	2.0	36.0
84-85	24.893439986426248	32.0	17.0	35.0	2.0	36.0
86-87	24.45138212175695	31.5	9.0	34.5	2.0	35.0
88-89	23.933240494197253	31.0	4.5	34.0	2.0	35.0
90-91	23.454145186083892	31.0	2.0	34.0	2.0	35.0
92-93	22.943033641876333	31.0	2.0	34.0	2.0	35.0
94-95	22.33197699537267	30.5	2.0	34.0	2.0	35.0
96-97	21.713644929119397	30.0	2.0	34.0	2.0	35.0
98	21.328267961064558	30.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	153975.0
3	26202.0
4	18891.0
5	15392.0
6	14448.0
7	14522.0
8	16720.0
9	18839.0
10	20885.0
11	22115.0
12	23221.0
13	23471.0
14	21776.0
15	22549.0
16	24821.0
17	26395.0
18	27325.0
19	26635.0
20	27902.0
21	29308.0
22	31819.0
23	35671.0
24	40524.0
25	47110.0
26	53999.0
27	62552.0
28	73067.0
29	84648.0
30	98546.0
31	116905.0
32	139445.0
33	169844.0
34	212587.0
35	272722.0
36	352867.0
37	399441.0
38	255380.0
39	30430.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03992655250562	17.489487301238636	12.410903480469464	25.05968266578628
2	16.12564916693997	18.99828352250407	39.31552552093077	25.56054178962519
3	20.10074172116002	22.792560510555766	28.390065392024532	28.716632376259682
4	13.27476362283046	14.753945298026395	35.96127152423757	36.01001955490558
5	14.457886587568941	36.753544425143616	33.99630699632862	14.792261990958824
6	34.65345723698868	34.27960359916442	16.67781685987844	14.389122303968463
7	30.302359217623614	31.07923190701377	20.014086837161077	18.604322038201538
8	27.33976503993692	34.40003223387642	19.32977777952487	18.930424946661777
9	27.00361060495239	15.753839481865251	18.974723144917817	38.26782676826454
10-11	26.205186391789454	25.117910506916097	26.96884096731327	21.708062133981187
12-13	26.700375904075425	22.23994017243664	27.103550263391636	23.956133660096302
14-15	23.451553175999862	23.956899396712753	24.51678468451279	28.074762742774595
16-17	23.823121104777353	27.087754170136186	25.335448565208473	23.753676159877987
18-19	23.81702904100953	26.18766484934742	26.788581116405368	23.206724993237685
20-21	24.40705179950897	25.555602359647413	26.665913794620987	23.37143204622263
22-23	24.06423608378842	25.288527617338936	26.558311431464197	24.088924867408444
24-25	23.852888358496212	25.52490356405743	26.64072207405533	23.98148600339103
26-27	23.61211065909962	25.79319493707068	26.590130837849667	24.00456356598003
28-29	23.71602418919922	26.016924835879955	26.424174909744423	23.842876065176398
30-31	23.874297626575512	25.838001894570944	26.27705338996618	24.010647088887364
32-33	23.59461516360023	25.87174664496098	26.245082066303688	24.2885561251351
34-35	24.03017928502921	25.80580643422436	26.33229556227974	23.831718718466693
36-37	24.191446522780037	25.498916067376864	26.284250649149442	24.025386760693657
38-39	23.892201437788117	25.729064292339277	26.33445655729989	24.044277712572715
40-41	24.08872227572891	25.587039283854534	26.456546683686398	23.86769175673016
42-43	23.922925826076128	25.57441186906484	26.297080947918378	24.205581356940655
44-45	23.884515903254325	25.70032272239296	26.398145760535513	24.017015613817204
46-47	24.038568748933116	25.67115589485424	26.18710960998741	24.103165746225237
48-49	23.93599283328104	25.767021779695742	26.079217370085132	24.21776801693808
50-51	23.850435658219546	25.9238139492515	26.20235371030911	24.02339668221984
52-53	24.013380336611124	25.62256144171465	26.274351853954197	24.08970636772003
54-55	23.6752139845937	25.61469051004529	26.518179188630576	24.191916316730435
56-57	23.57355122267508	25.79472769945561	26.41714727186269	24.214573806006616
58-59	23.589147578765704	25.47156142727433	26.505242824018758	24.434048169941207
60-61	23.47219688729355	25.5022936311745	26.670845627741414	24.35466385379053
62-63	23.43308890119336	25.594908119640902	26.69283824409458	24.279164735071156
64-65	23.356188747528346	25.495144883494643	26.578608003595967	24.57005836538104
66-67	23.39806240650626	25.519985649893606	26.522600594739504	24.55935134886063
68-69	23.667783419104527	25.232634828531626	26.582819775235066	24.51676197712878
70-71	23.443068257460567	25.16293208876447	26.587978560351576	24.806021093423386
72-73	23.468262441512504	25.231767886099682	26.553279203887854	24.746690468499967
74-75	23.356976854833686	25.375504360703754	26.52129946621706	24.7462193182455
76-77	23.44584992722054	25.09437828984616	26.617792043512868	24.841979739420434
78-79	23.405698745384615	25.18098748474275	26.550528339983497	24.862785429889136
80-81	23.50482105289803	25.153122938698065	26.516999058324544	24.825056950079365
82-83	23.416815345459387	25.115920149832068	26.653523214847773	24.813741289860776
84-85	23.304555094409025	25.11356219503556	26.563437123352944	25.01844558720247
86-87	23.35474846058744	25.10090878299595	26.587165615631086	24.95717714078552
88-89	23.379697867930705	24.917634049054087	26.525905384256337	25.176762698758875
90-91	23.327047063520805	24.995409812136803	26.519894622107955	25.15764850223444
92-93	23.524222935054386	24.778131125872875	26.657415590227828	25.04023034884491
94-95	23.324214080862195	24.585573697630046	26.80128248117305	25.28892974033471
96-97	23.533940635877684	24.40465672766375	26.75463518700269	25.30676744945588
98	23.467072615913807	24.332991024585745	26.654883093454234	25.545053266046214
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	39.5
2	60.5
3	102.0
4	120.0
5	139.0
6	213.0
7	420.5
8	645.5
9	782.5
10	870.5
11	978.0
12	1097.5
13	1275.5
14	1408.5
15	1505.0
16	1687.5
17	1789.0
18	1813.0
19	1804.0
20	1891.0
21	2024.0
22	2250.0
23	2673.0
24	3257.0
25	4131.0
26	5526.0
27	7693.5
28	10550.0
29	14349.0
30	19645.5
31	27369.5
32	33732.0
33	38280.0
34	44750.5
35	52616.5
36	60575.5
37	67442.5
38	73469.5
39	78938.0
40	84780.5
41	90931.0
42	97640.0
43	105150.5
44	111668.0
45	116907.5
46	121617.0
47	126409.0
48	129623.0
49	130203.5
50	130077.0
51	130069.5
52	129881.5
53	130628.5
54	128219.5
55	122528.0
56	114150.5
57	104308.0
58	96202.0
59	84476.0
60	70910.0
61	58566.5
62	47717.0
63	37262.5
64	27601.0
65	20897.5
66	15783.0
67	12073.0
68	9384.5
69	7155.0
70	5272.0
71	4024.0
72	3346.5
73	2783.0
74	2209.5
75	1646.5
76	1155.0
77	721.0
78	430.5
79	257.5
80	148.5
81	91.5
82	63.0
83	40.5
84	23.5
85	11.0
86	9.5
87	7.0
88	5.0
89	6.0
90	3.5
91	3.0
92	3.5
93	2.5
94	0.5
95	0.5
96	2.0
97	2.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.38870587834524695
2	0.025221498805581874
3	0.01935831921311544
4	0.01703270049208127
5	0.02269934892502369
6	0.022502817765499675
7	0.014936368123825112
8	0.008581860632548639
9	0.0
10-11	0.0
12-13	0.005814046802585433
14-15	0.0030626105692492284
16-17	0.005666648432942422
18-19	0.03640739730182371
20-21	0.07895639333877288
22-23	0.09770874147668927
24-25	0.06426568916435278
26-27	0.07423964551019653
28-29	0.05746898656414728
30-31	0.052424686803030904
32-33	0.01971862633890947
34-35	0.3450104505444077
36-37	0.5271129474400342
38-39	0.4806169506159778
40-41	0.4883471762239223
42-43	0.4384446393014496
44-45	0.41163451362304865
46-47	0.4114707376567786
48-49	0.40056325830319583
50-51	0.3960921744240245
52-53	0.4590312782615902
54-55	0.46066903792429026
56-57	0.429764513089139
58-59	0.41443508264626583
60-61	0.429764513089139
62-63	0.4095218036581655
64-65	0.45831066401000214
66-67	0.47973256039811973
68-69	0.4220834202710754
70-71	0.37919049470495925
72-73	0.36582637585732625
74-75	0.3475162228283389
76-77	0.34525611449381266
78-79	0.34538713526682874
80-81	0.3613716695747819
82-83	0.35632736981366553
84-85	0.3568842080989836
86-87	0.3431270269323025
88-89	0.3864293924140938
90-91	0.3647782096731982
92-93	0.3531828712612813
94-95	0.3614535575579169
96-97	0.3612734039950199
98	0.3558196643182285
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3052951.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.884315280865174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.07689132941378	45.38619969910185
2	12.126050386616729	14.28068348154393
3	4.552576610780569	8.04226069468487
4	2.141414342583426	5.043828691825962
5	1.1796694728686643	3.4732014583805233
6	0.7220868203334901	2.5511752793204807
7	0.46783468835183306	1.9283687701764247
8	0.32464863169616964	1.5293369907438987
9	0.24781962076764133	1.313341981385961
>10	1.0848606969384567	10.991053681565628
>50	0.05157219781257345	2.039621096257759
>100	0.022620292902347256	2.3167268428993006
>500	0.0011170908203370347	0.4266763140505321
>1k	8.378181140449611E-4	0.6775250180627553
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.275519325400244E-5
2	0.0	0.0	0.0	0.0	3.275519325400244E-5
3	0.0	0.0	0.0	0.0	3.275519325400244E-5
4	0.0	0.0	0.0	0.0	3.275519325400244E-5
5	3.275519325400244E-5	0.0	0.0	0.0	3.275519325400244E-5
6	3.275519325400244E-5	0.0	0.0	0.0	3.275519325400244E-5
7	3.275519325400244E-5	0.0	0.0	0.0	3.275519325400244E-5
8	3.275519325400244E-5	0.0	0.0	0.0	3.275519325400244E-5
9	3.275519325400244E-5	0.0	0.0	0.0	3.275519325400244E-5
10-11	3.275519325400244E-5	0.0	0.0	0.0	3.275519325400244E-5
12-13	3.275519325400244E-5	0.0	0.0	1.637759662700122E-5	9.826557976200733E-5
14-15	3.275519325400244E-5	0.0	0.0	4.9132789881003666E-5	1.47398369643011E-4
16-17	3.275519325400244E-5	1.637759662700122E-5	0.0	6.551038650800488E-5	1.637759662700122E-4
18-19	3.275519325400244E-5	6.551038650800488E-5	0.0	1.3102077301600977E-4	2.2928635277801707E-4
20-21	3.275519325400244E-5	6.551038650800488E-5	0.0	2.1290875615101587E-4	2.2928635277801707E-4
22-23	3.275519325400244E-5	6.551038650800488E-5	0.0	2.620415460320195E-4	2.9479673928602194E-4
24-25	3.275519325400244E-5	6.551038650800488E-5	0.0	3.76684722421028E-4	2.9479673928602194E-4
26-27	3.275519325400244E-5	6.551038650800488E-5	0.0	8.516350246040635E-4	3.603071257940268E-4
28-29	3.275519325400244E-5	6.551038650800488E-5	0.0	0.004258175123020317	4.258175123020317E-4
30-31	3.275519325400244E-5	6.551038650800488E-5	0.0	0.020586638960140534	4.258175123020317E-4
32-33	3.275519325400244E-5	6.551038650800488E-5	0.0	0.05530714380938312	4.258175123020317E-4
34-35	3.275519325400244E-5	6.551038650800488E-5	0.0	0.10188502861657459	4.258175123020317E-4
36-37	3.275519325400244E-5	6.551038650800488E-5	0.0	0.1772547282940342	4.258175123020317E-4
38-39	3.275519325400244E-5	6.551038650800488E-5	0.0	0.3080462149572659	4.4219510892903294E-4
40-41	3.275519325400244E-5	9.826557976200732E-5	0.0	0.4647961922742946	4.5857270555603414E-4
42-43	3.275519325400244E-5	9.826557976200732E-5	0.0	0.6300297646441099	4.7495030218303535E-4
44-45	3.275519325400244E-5	9.826557976200732E-5	0.0	0.8248412765222894	4.913278988100366E-4
46-47	3.275519325400244E-5	9.826557976200732E-5	0.0	1.0602037176489238	4.913278988100366E-4
48-49	3.275519325400244E-5	9.826557976200732E-5	0.0	1.3194774498509803	5.240830920640391E-4
50-51	3.275519325400244E-5	9.826557976200732E-5	0.0	1.6064948307391766	5.240830920640391E-4
52-53	3.275519325400244E-5	9.826557976200732E-5	0.0	1.8960507391045582	5.568382853180415E-4
54-55	3.275519325400244E-5	9.826557976200732E-5	0.0	2.2200487331765233	5.568382853180415E-4
56-57	3.275519325400244E-5	9.826557976200732E-5	0.0	2.597519580235647	5.568382853180415E-4
58-59	3.275519325400244E-5	9.826557976200732E-5	0.0	3.0042899476604763	5.568382853180415E-4
60-61	3.275519325400244E-5	9.826557976200732E-5	0.0	3.442783719751807	5.568382853180415E-4
62-63	3.275519325400244E-5	9.826557976200732E-5	0.0	3.9008978526022857	6.223486718260463E-4
64-65	3.275519325400244E-5	9.826557976200732E-5	0.0	4.377322138481752	6.223486718260463E-4
66-67	3.275519325400244E-5	9.826557976200732E-5	0.0	4.920550641002754	6.223486718260463E-4
68-69	3.275519325400244E-5	9.826557976200732E-5	0.0	5.524245230270646	6.223486718260463E-4
70-71	3.275519325400244E-5	9.826557976200732E-5	0.0	6.174828878681643	6.551038650800488E-4
72-73	3.275519325400244E-5	1.3102077301600977E-4	0.0	6.833077242314076	7.042366549610524E-4
74-75	3.275519325400244E-5	1.3102077301600977E-4	0.0	7.497156030345721	7.697470414690573E-4
76-77	3.275519325400244E-5	1.3102077301600977E-4	0.0	8.21375777076016	7.861246380960585E-4
78-79	3.275519325400244E-5	1.3102077301600977E-4	0.0	9.006515335490153	7.861246380960585E-4
80-81	3.275519325400244E-5	1.3102077301600977E-4	0.0	9.841608987500946	7.861246380960585E-4
82-83	3.275519325400244E-5	1.3102077301600977E-4	0.0	10.692326866693897	8.18879831350061E-4
84-85	3.275519325400244E-5	1.3102077301600977E-4	1.637759662700122E-5	11.541816426139825	8.18879831350061E-4
86	3.275519325400244E-5	1.3102077301600977E-4	3.275519325400244E-5	12.191155377207167	8.18879831350061E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2620	0.0	44.616425	1
GTATCAA	4980	0.0	44.26582	1
ATCAACG	5925	0.0	36.22323	3
TCAACGC	5930	0.0	36.11535	4
CAACGCA	6170	0.0	34.85919	5
TATCAAC	6485	0.0	33.66152	2
AACGCAG	6490	0.0	33.27682	6
GTACATG	9755	0.0	26.985538	1
ACGCAGA	7870	0.0	26.85516	7
CGCAGAG	7920	0.0	26.221241	8
TACATGG	10015	0.0	25.505919	2
ACATGGG	10245	0.0	24.753853	3
GCAGAGT	8750	0.0	22.790897	9
CATGGGG	6990	0.0	21.584816	4
GAGTACA	7325	0.0	21.487247	1
GTACTAG	485	0.0	20.875807	1
GTATAGG	620	0.0	20.041693	1
AGTACAT	7420	0.0	19.839827	2
AGAGTAC	7735	0.0	18.521538	10-11
GTGTAGC	2170	0.0	18.239002	1
>>END_MODULE