FastQCFastQC Report
Mon 15 Aug 2016
SRR2920899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052951
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25600.049.2276881
GTATCAA48450.044.562091
ATCAACG60450.034.866863
TCAACGC60450.034.788334
CAACGCA61750.033.666595
TATCAAC66500.032.1220132
AACGCAG65350.031.9572286
ACGCAGA78950.026.21227
GTACATG100950.025.5797921
CGCAGAG83400.024.7554598
TACATGG103050.024.4140452
ACATGGG102400.023.5963043
GAGTACA75250.022.2453731
GCAGAGT93350.021.4050149
AGTACAT76500.020.4148562
CATGGGG71750.020.0468834
AGAGTAC80650.019.5366210-11
TATAGCG2352.5487207E-618.1776055
CAGAGTA86950.017.71172310-11
GTGGTAT16200.017.613261
Produced by FastQC (version 0.11.5)