##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920899_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3052951
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.24085384927567	31.0	30.0	34.0	26.0	34.0
2	30.256740118003858	31.0	30.0	34.0	26.0	34.0
3	30.40145387200777	31.0	30.0	34.0	27.0	34.0
4	34.03821843193684	35.0	35.0	37.0	30.0	37.0
5	33.55195153803648	35.0	35.0	37.0	28.0	37.0
6	33.490263682581215	35.0	35.0	37.0	28.0	37.0
7	33.304737612886676	35.0	35.0	37.0	27.0	37.0
8	33.23334308346252	35.0	33.0	37.0	27.0	37.0
9	34.56847915344858	38.0	34.0	39.0	27.0	39.0
10-11	34.37516324369438	38.0	34.0	39.0	27.0	39.0
12-13	34.196875252829145	37.0	34.0	39.0	26.0	39.0
14-15	35.09988335875683	38.0	34.0	40.0	26.0	41.0
16-17	34.97400793527312	38.0	34.0	40.0	25.0	41.0
18-19	34.88341853505018	38.5	34.0	40.0	25.0	41.0
20-21	34.76230457023385	38.0	34.0	40.0	25.0	41.0
22-23	34.614371144509036	38.0	34.0	40.0	24.0	41.0
24-25	34.43606415563171	38.0	33.0	40.0	23.5	41.0
26-27	34.248203787089935	38.0	33.0	40.0	23.0	41.0
28-29	34.19042575527743	38.0	33.0	40.0	22.5	41.0
30-31	34.179779662366016	38.0	33.0	40.0	22.5	41.0
32-33	34.02684451863132	38.0	33.0	40.0	21.5	41.0
34-35	33.85622386340299	38.0	33.0	40.0	20.0	41.0
36-37	33.659805054191835	38.0	33.0	40.0	18.5	41.0
38-39	33.466339453204455	38.0	32.0	40.0	18.0	41.0
40-41	33.23413428515558	38.0	32.0	40.0	16.0	41.0
42-43	33.25642386661299	38.0	32.0	40.0	15.5	41.0
44-45	33.10621723047635	38.0	32.0	40.0	15.0	41.0
46-47	32.852427045176945	38.0	31.5	40.0	12.5	41.0
48-49	32.58225238465996	37.0	31.0	40.0	9.0	41.0
50-51	32.31236138411655	37.0	31.0	40.0	8.0	41.0
52-53	32.047723170139314	37.0	31.0	40.0	7.5	41.0
54-55	31.758437655894248	36.0	30.0	40.0	6.0	41.0
56-57	31.42602534400323	36.0	30.0	39.0	2.0	41.0
58-59	31.091337692612818	35.5	29.0	39.0	2.0	40.5
60-61	30.757730471271895	35.0	29.0	39.0	2.0	40.0
62-63	30.438080401552465	35.0	28.5	39.0	2.0	40.0
64-65	30.029102825430215	35.0	28.0	38.0	2.0	40.0
66-67	29.597468154582238	34.0	28.0	38.0	2.0	40.0
68-69	29.155523786657568	34.0	27.5	37.0	2.0	39.5
70-71	28.72719526124068	34.0	26.0	36.5	2.0	39.0
72-73	28.290441281239037	34.0	26.0	36.0	2.0	39.0
74-75	27.84951412584087	33.0	26.0	35.5	2.0	38.5
76-77	26.422065896242685	31.5	24.0	34.5	2.0	36.0
78-79	27.256602054864295	33.0	26.0	35.0	2.0	37.0
80-81	27.28777369830043	33.0	26.0	35.0	2.0	37.0
82-83	26.94612262037615	33.0	25.0	35.0	2.0	36.0
84-85	26.62976018940363	33.0	25.0	35.0	2.0	36.0
86-87	26.09258451904403	32.5	23.5	35.0	2.0	35.0
88-89	25.726980387172937	32.0	22.0	35.0	2.0	35.0
90-91	25.356893707105026	32.0	20.0	35.0	2.0	35.0
92-93	25.047339279274382	32.0	19.0	34.0	2.0	35.0
94-95	24.667919334440676	32.0	16.5	34.0	2.0	35.0
96-97	24.14200768371323	31.5	4.5	34.0	2.0	35.0
98-99	23.544047873680253	31.0	2.0	34.0	2.0	35.0
100-101	21.35781429181143	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73953.0
3	33925.0
4	17981.0
5	12970.0
6	10836.0
7	10989.0
8	12293.0
9	14284.0
10	16247.0
11	18009.0
12	19674.0
13	21756.0
14	23359.0
15	25078.0
16	26436.0
17	27507.0
18	26646.0
19	25355.0
20	25587.0
21	26448.0
22	28445.0
23	32466.0
24	37300.0
25	44245.0
26	52082.0
27	61702.0
28	72926.0
29	86110.0
30	101230.0
31	119363.0
32	142721.0
33	175704.0
34	222011.0
35	288617.0
36	384676.0
37	450598.0
38	262929.0
39	20491.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.131016837792586	18.73374260027817	13.080614386129119	25.054626175800127
2	16.30458530123805	19.994719862847454	38.00277174445315	25.697923091461345
3	19.890381825829557	23.448926749560048	28.73921082252614	27.92148060208426
4	13.812832882664777	15.663282120699677	35.47339199122556	35.05049300540999
5	14.462149763082854	36.919995217434746	34.008231908554734	14.60962311092767
6	34.66004257501453	34.789269417472546	16.355311187155618	14.19537682035731
7	30.176078513697913	31.181215230397818	20.038443371470557	18.604262884433716
8	27.39766415582439	34.56583024397411	19.232376222297436	18.804129377904065
9	26.860362250124396	15.580055151972976	18.92351640897481	38.63606618892781
10-11	26.248144558582432	25.228018645167456	26.968333745152652	21.555503051097457
12-13	26.63374719868619	22.29681825843378	27.140016829198068	23.929417713681957
14-15	23.382087485290214	24.0155695411137	24.50900836570156	28.093334607894526
16-17	23.820160711967915	27.191844175731678	25.33234193968778	23.655653172612627
18-19	23.825753614698556	26.24295573459178	26.783681267294117	23.147609383415542
20-21	24.450434719068117	25.533500983108816	26.597447374064835	23.418616923758236
22-23	24.067240692568316	25.368846883262275	26.580005474718764	23.983906949450642
24-25	23.82658281697471	25.62123628478743	26.632125863683946	23.920055034553915
26-27	23.635552848891443	25.89070263062841	26.537920211242167	23.935824309237976
28-29	23.727433666017028	25.974756672311067	26.503986715590255	23.793822946081647
30-31	23.857290758857488	25.801875356298037	26.35049242846191	23.990341456382566
32-33	23.57526182803124	25.842988208707503	26.3008749636094	24.280874999651864
34-35	24.03129054239998	25.823018909958193	26.34536882936482	23.800321718277008
36-37	24.183779781406443	25.57443058212985	26.290232747097214	23.9515568893665
38-39	23.91074919249831	25.76277700633616	26.34875226326554	23.977721537899992
40-41	24.05088133149859	25.594489220889848	26.48758272721316	23.867046720398402
42-43	23.918487205560158	25.60458773180422	26.331105269778703	24.14581979285692
44-45	23.88796103733349	25.628781654914036	26.46476544012791	24.01849186762457
46-47	23.964339509936945	25.70773287267935	26.234176624147704	24.093750993236
48-49	23.861147313261736	25.79909479791338	26.159613075490668	24.180144813334216
50-51	23.81924645235856	25.922344186985118	26.21906614237871	24.039343218277615
52-53	24.01434713226875	25.669239932512987	26.23632148936585	24.080091445852418
54-55	23.677328126405055	25.630810723778207	26.442004593134477	24.249856556682264
56-57	23.587650593579095	25.80515982978874	26.25687710063681	24.350312475995352
58-59	23.509693417124026	25.559175130083577	26.399424143708572	24.53170730908382
60-61	23.41553336318017	25.621824166078166	26.528843726569534	24.433798744172126
62-63	23.369999620004535	25.710018252885675	26.502767415257733	24.417214711852058
64-65	23.236301599837788	25.565269575238304	26.384646352940482	24.81378247198343
66-67	23.268381901768873	25.643458928747854	26.3118917203432	24.776267449140075
68-69	23.559637406848182	25.41787895234912	26.35047491218186	24.67200872862084
70-71	23.272080426158652	25.33060008541127	26.30663758904993	25.09068189938015
72-73	23.265839198701926	25.36164243100788	26.24960328168991	25.122915088600283
74-75	23.125209610461393	25.46922418476078	26.17946556749645	25.22610063728137
76-77	23.153967867995124	25.185996762532465	26.420775879011515	25.239259490460896
78-79	23.127341545293273	25.224133530920405	26.372940296567464	25.275584627218862
80-81	23.099735672515003	25.26780418504595	26.25405676431512	25.37840337812393
82-83	22.957643749392933	25.291117018288205	26.300012563079516	25.45122666923934
84-85	22.800382303633278	25.113024449250993	26.323537962963723	25.76305528415201
86-87	22.753487247800187	25.196876124865675	26.26850362978552	25.78113299754861
88-89	22.708141094975403	25.063338744985597	26.19011165714725	26.038408502891752
90-91	22.52797707840282	25.09856102868681	26.251028798475044	26.122433094435326
92-93	22.641811170016936	24.935062245574173	26.334272802339402	26.08885378206949
94-95	22.346513004019265	24.70291162088511	26.454004795079072	26.496570580016552
96-97	22.46203237879326	24.51283341383237	26.478205560968565	26.546928646405803
98-99	22.246077851715853	24.54849640746121	26.37999511932273	26.825430621500203
100-101	22.328026573598144	24.365579256712966	26.485351420308767	26.82104274938012
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	31.5
2	39.0
3	73.0
4	70.5
5	65.5
6	121.0
7	247.5
8	417.5
9	480.0
10	421.0
11	392.0
12	400.5
13	422.5
14	487.0
15	560.0
16	598.0
17	677.5
18	797.0
19	911.0
20	1068.0
21	1322.0
22	1626.5
23	2016.5
24	2529.0
25	3207.5
26	4387.0
27	6117.5
28	8395.5
29	11557.5
30	15805.5
31	21494.0
32	29242.5
33	35073.0
34	39119.0
35	45592.5
36	53947.0
37	61456.5
38	67679.5
39	73066.5
40	77998.5
41	84349.5
42	90051.0
43	96791.0
44	104426.5
45	110724.0
46	116138.0
47	120677.5
48	124922.5
49	128692.0
50	129512.0
51	128854.5
52	129249.5
53	130370.0
54	128825.0
55	122348.0
56	113787.0
57	104338.5
58	95959.5
59	85384.5
60	72118.5
61	59757.0
62	49160.5
63	38105.5
64	28028.5
65	21441.0
66	16336.0
67	12492.5
68	9653.5
69	7333.5
70	5556.0
71	4266.5
72	3338.5
73	2894.5
74	2273.0
75	1699.0
76	1263.5
77	787.0
78	465.0
79	262.5
80	158.0
81	96.0
82	58.5
83	32.0
84	16.0
85	9.5
86	6.5
87	3.0
88	2.0
89	1.5
90	1.0
91	0.0
92	1.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19250227075377233
2	0.0
3	0.011628093605170866
4	0.014707081771047096
5	0.006420017877784479
6	0.10727325790685799
7	0.14120763811800452
8	0.12365085453385921
9	0.14032324790014644
10-11	0.11380791896103148
12-13	0.1142828692632145
14-15	0.11580598574952561
16-17	0.06639477672586294
18-19	0.0792675676746859
20-21	0.03775036022523781
22-23	0.08404982588977027
24-25	0.05820597841236234
26-27	0.06698437020443498
28-29	0.041058634743892056
30-31	0.023944046268675783
32-33	0.03260779488435943
34-35	0.01935831921311544
36-37	0.023288942403595733
38-39	0.030953657625032303
40-41	0.02459915013375583
42-43	0.012283197470250914
44-45	0.026155021813320946
46-47	0.03465499446273458
48-49	0.00859823822917564
50-51	0.014084733099221048
52-53	0.007058744146237526
54-55	0.011513450428781857
56-57	0.005126187744251382
58-59	0.0034720504849242583
60-61	0.02297776806768271
62-63	0.00926971969088269
64-65	0.006682059423816497
66-67	0.04128792109667007
68-69	0.09025693501140372
70-71	0.02163480514426861
72-73	0.0443013988760383
74-75	0.08680126212310647
76-77	0.2512159546615717
78-79	0.08128201205980705
80-81	0.01596815671132619
82-83	0.011644471201797867
84-85	0.013216720477989984
86-87	0.26672553866734183
88-89	0.6244941369841834
90-91	0.26652900750781783
92-93	0.3539853734960044
94-95	9.66278200993072E-4
96-97	0.003914245593853291
98-99	0.003144498552384234
100-101	0.004798635811711357
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3052951.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.279821760214524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.03581615858998	42.23002359037669
2	12.945668592491039	14.571598419044054
3	5.0131405771475155	8.464159744222835
4	2.3673739873353745	5.329415441880129
5	1.3192412882508644	3.7123332280737213
6	0.8322393769181443	2.8102970276871093
7	0.5312659590802558	2.0929687439014346
8	0.37536082154853107	1.6900192106015213
9	0.26813513653683674	1.3581537922749558
>10	1.2247882839724462	11.819711316717422
>50	0.05929162869009249	2.246169998998832
>100	0.02557604277001598	2.5328157728233345
>500	0.0012846451868196655	0.5039939537597149
>1k	8.175014820849527E-4	0.6383397596382759
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.275519325400244E-5
2	0.0	0.0	0.0	0.0	3.275519325400244E-5
3	0.0	0.0	0.0	0.0	3.275519325400244E-5
4	0.0	0.0	0.0	0.0	3.275519325400244E-5
5	0.0	3.275519325400244E-5	0.0	0.0	3.275519325400244E-5
6	0.0	3.275519325400244E-5	0.0	0.0	3.275519325400244E-5
7	0.0	3.275519325400244E-5	0.0	0.0	3.275519325400244E-5
8	0.0	3.275519325400244E-5	0.0	0.0	3.275519325400244E-5
9	0.0	3.275519325400244E-5	0.0	3.275519325400244E-5	3.275519325400244E-5
10-11	0.0	3.275519325400244E-5	0.0	3.275519325400244E-5	3.275519325400244E-5
12-13	0.0	3.275519325400244E-5	0.0	4.9132789881003666E-5	6.551038650800488E-5
14-15	0.0	3.275519325400244E-5	0.0	8.18879831350061E-5	6.551038650800488E-5
16-17	1.637759662700122E-5	3.275519325400244E-5	0.0	9.826557976200732E-5	6.551038650800488E-5
18-19	3.275519325400244E-5	3.275519325400244E-5	0.0	1.637759662700122E-4	6.551038650800488E-5
20-21	3.275519325400244E-5	3.275519325400244E-5	0.0	2.2928635277801707E-4	9.826557976200732E-5
22-23	3.275519325400244E-5	3.275519325400244E-5	0.0	2.7841914265902074E-4	1.3102077301600977E-4
24-25	3.275519325400244E-5	3.275519325400244E-5	0.0	3.76684722421028E-4	1.3102077301600977E-4
26-27	3.275519325400244E-5	3.275519325400244E-5	0.0	8.843902178580659E-4	1.9653115952401464E-4
28-29	3.275519325400244E-5	3.275519325400244E-5	0.0	0.00430730791290132	1.9653115952401464E-4
30-31	3.275519325400244E-5	3.275519325400244E-5	0.0	0.020734037329783545	1.9653115952401464E-4
32-33	3.275519325400244E-5	3.275519325400244E-5	0.0	0.0551269902464861	1.9653115952401464E-4
34-35	3.275519325400244E-5	3.275519325400244E-5	0.0	0.10175400784355858	2.2928635277801707E-4
36-37	3.275519325400244E-5	3.275519325400244E-5	0.0	0.1769763091513752	2.2928635277801707E-4
38-39	3.275519325400244E-5	3.275519325400244E-5	0.0	0.3074729990753209	2.2928635277801707E-4
40-41	3.275519325400244E-5	3.275519325400244E-5	0.0	0.4629619014520705	2.2928635277801707E-4
42-43	3.275519325400244E-5	3.275519325400244E-5	0.0	0.6287850673004578	2.2928635277801707E-4
44-45	3.275519325400244E-5	3.275519325400244E-5	0.0	0.8235965791786373	2.7841914265902074E-4
46-47	3.275519325400244E-5	3.275519325400244E-5	0.0	1.059122796271542	3.275519325400244E-4
48-49	3.275519325400244E-5	3.275519325400244E-5	0.0	1.316463972071612	3.439295291670256E-4
50-51	3.275519325400244E-5	3.275519325400244E-5	0.0	1.598584451568335	4.913278988100366E-4
52-53	3.275519325400244E-5	3.275519325400244E-5	0.0	1.883243458542243	5.404606886910403E-4
54-55	3.275519325400244E-5	3.275519325400244E-5	0.0	2.202016999290195	5.895934785720439E-4
56-57	3.275519325400244E-5	3.275519325400244E-5	0.0	2.57347726838721	5.895934785720439E-4
58-59	3.275519325400244E-5	3.275519325400244E-5	0.0	2.9755308879834628	5.895934785720439E-4
60-61	3.275519325400244E-5	3.275519325400244E-5	0.0	3.4097664849517733	5.895934785720439E-4
62-63	3.275519325400244E-5	3.275519325400244E-5	0.0	3.8588074292708923	5.895934785720439E-4
64-65	3.275519325400244E-5	3.275519325400244E-5	0.0	4.328303991777137	5.895934785720439E-4
66-67	3.275519325400244E-5	3.275519325400244E-5	0.0	4.863900534269957	6.059710751990451E-4
68-69	3.275519325400244E-5	3.275519325400244E-5	0.0	5.455983407529305	6.223486718260463E-4
70-71	3.275519325400244E-5	3.275519325400244E-5	0.0	6.090009305750403	6.223486718260463E-4
72-73	3.275519325400244E-5	3.275519325400244E-5	0.0	6.738005293894334	6.551038650800488E-4
74-75	3.275519325400244E-5	3.275519325400244E-5	0.0	7.394910694603352	6.878590583340512E-4
76-77	3.275519325400244E-5	3.275519325400244E-5	0.0	8.10397874056937	6.878590583340512E-4
78-79	3.275519325400244E-5	3.275519325400244E-5	0.0	8.887876025524157	7.042366549610524E-4
80-81	3.275519325400244E-5	3.275519325400244E-5	0.0	9.71422404093613	7.206142515880536E-4
82-83	3.275519325400244E-5	3.275519325400244E-5	0.0	10.553559490473315	7.206142515880536E-4
84-85	3.275519325400244E-5	3.275519325400244E-5	0.0	11.393206114346414	7.206142515880536E-4
86-87	3.275519325400244E-5	3.275519325400244E-5	0.0	12.259531843124897	8.025022347230598E-4
88-89	3.275519325400244E-5	3.275519325400244E-5	0.0	13.17490847380125	8.843902178580659E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2560	0.0	49.227688	1
GTATCAA	4845	0.0	44.56209	1
ATCAACG	6045	0.0	34.86686	3
TCAACGC	6045	0.0	34.78833	4
CAACGCA	6175	0.0	33.66659	5
TATCAAC	6650	0.0	32.122013	2
AACGCAG	6535	0.0	31.957228	6
ACGCAGA	7895	0.0	26.2122	7
GTACATG	10095	0.0	25.579792	1
CGCAGAG	8340	0.0	24.755459	8
TACATGG	10305	0.0	24.414045	2
ACATGGG	10240	0.0	23.596304	3
GAGTACA	7525	0.0	22.245373	1
GCAGAGT	9335	0.0	21.405014	9
AGTACAT	7650	0.0	20.414856	2
CATGGGG	7175	0.0	20.046883	4
AGAGTAC	8065	0.0	19.53662	10-11
TATAGCG	235	2.5487207E-6	18.177605	5
CAGAGTA	8695	0.0	17.711723	10-11
GTGGTAT	1620	0.0	17.61326	1
>>END_MODULE