FastQCFastQC Report
Mon 15 Aug 2016
SRR2920898_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920898_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4893842
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67800.043.9186821
GGTATCA37000.038.9329531
TCAACGC82750.035.308084
ATCAACG83900.034.769453
CAACGCA85050.034.461115
AACGCAG89950.032.7827876
TATCAAC90050.032.447122
ACGCAGA109000.026.6279567
GTACATG139150.025.8310781
CGCAGAG113050.025.5096918
TACATGG145500.024.085232
ACATGGG149250.023.1717953
GCAGAGT128150.021.61019
CATGGGG104100.020.0476464
GAGTACA103800.019.0653951
TAAGGCG18600.018.9880545
AGAGTAC110250.018.070210-11
AGTACAT106200.017.2765062
CAGAGTA120300.016.75116310-11
GTATTAA33050.016.5710581
Produced by FastQC (version 0.11.5)