##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920898_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4893842
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.566392417246	31.0	29.0	33.0	22.0	34.0
2	28.586202210860097	31.0	28.0	34.0	16.0	34.0
3	28.67078871773956	31.0	28.0	34.0	20.0	34.0
4	32.1425996180506	35.0	32.0	37.0	22.0	37.0
5	32.10555162998724	35.0	32.0	37.0	22.0	37.0
6	32.2310191052347	35.0	32.0	37.0	23.0	37.0
7	32.06309459929438	35.0	32.0	37.0	22.0	37.0
8	32.00493722519035	35.0	32.0	37.0	22.0	37.0
9	33.267305932639424	37.0	33.0	39.0	21.0	39.0
10-11	33.080440377928014	37.0	32.0	39.0	19.0	39.0
12-13	32.905801311117116	37.0	32.0	39.0	18.0	39.0
14-15	33.66338001921599	38.0	32.0	40.0	17.0	41.0
16-17	33.55699550578053	38.0	32.0	40.0	17.0	41.0
18-19	33.43758829974486	38.0	32.0	40.0	17.5	41.0
20-21	33.33875490871998	38.0	32.0	40.0	16.5	41.0
22-23	33.12062424573576	38.0	32.0	40.0	15.5	41.0
24-25	32.92960520588936	38.0	32.0	40.0	12.0	41.0
26-27	32.96579282698542	38.0	32.0	40.0	10.5	41.0
28-29	32.807718557321635	38.0	31.5	40.0	10.0	41.0
30-31	32.55889677271968	38.0	31.0	40.0	9.0	41.0
32-33	32.31615395429603	37.0	30.5	40.0	8.5	41.0
34-35	31.99490328866359	37.0	30.0	40.0	7.0	41.0
36-37	31.69070681072254	37.0	30.0	40.0	2.0	41.0
38-39	31.486470037242725	37.0	30.0	40.0	2.0	41.0
40-41	31.2904543097223	37.0	30.0	40.0	2.0	41.0
42-43	31.0556685320041	36.0	29.5	40.0	2.0	41.0
44-45	30.781565792275273	36.0	29.0	39.0	2.0	41.0
46-47	30.530846929672023	36.0	28.0	39.0	2.0	40.0
48-49	30.231711403841807	35.5	28.0	39.0	2.0	40.0
50-51	29.08475947936202	34.0	25.5	38.0	2.0	39.5
52-53	29.5261286531114	34.5	27.5	38.5	2.0	39.5
54-55	30.171801929853885	35.5	28.0	39.0	2.0	40.0
56-57	30.1216432814954	35.0	28.0	39.0	2.0	41.0
58-59	29.876656929259262	35.0	28.0	39.0	2.0	40.0
60-61	29.550080591077524	35.0	27.5	39.0	2.0	40.0
62-63	29.15982361097886	34.5	26.5	38.5	2.0	40.0
64-65	28.742254040894657	34.0	26.0	38.0	2.0	40.0
66-67	28.360821620313857	34.0	26.0	37.5	2.0	40.0
68-69	27.919220113767466	34.0	25.5	37.0	2.0	39.5
70-71	27.58186788621292	34.0	25.0	36.5	2.0	39.0
72-73	27.12562205727116	33.0	24.0	36.0	2.0	39.0
74-75	26.686083960209587	33.0	23.0	35.5	2.0	38.5
76-77	26.265890684660437	33.0	21.5	35.0	2.0	37.5
78-79	25.802803400681917	32.0	20.0	35.0	2.0	37.0
80-81	25.35756579390998	32.0	18.5	35.0	2.0	36.0
82-83	24.876781269195043	32.0	16.5	35.0	2.0	36.0
84-85	24.48050662444762	31.0	10.0	34.5	2.0	35.5
86-87	24.022340014246474	31.0	4.5	34.0	2.0	35.0
88-89	23.48392663678149	31.0	2.0	34.0	2.0	35.0
90-91	22.983587741492265	31.0	2.0	34.0	2.0	35.0
92-93	22.4410331391982	30.0	2.0	34.0	2.0	35.0
94-95	21.79777452970488	30.0	2.0	34.0	2.0	35.0
96-97	21.146680154365427	29.0	2.0	34.0	2.0	35.0
98	20.74492127044559	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	250907.0
3	42845.0
4	31601.0
5	25786.0
6	23966.0
7	24341.0
8	28334.0
9	31987.0
10	34974.0
11	37447.0
12	39532.0
13	38634.0
14	36333.0
15	38345.0
16	40839.0
17	43739.0
18	44733.0
19	45189.0
20	47005.0
21	49929.0
22	54759.0
23	61202.0
24	69583.0
25	80165.0
26	92304.0
27	106051.0
28	122896.0
29	142474.0
30	165481.0
31	194536.0
32	230152.0
33	279811.0
34	346435.0
35	438389.0
36	555210.0
37	603578.0
38	357979.0
39	36367.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.817426136414916	17.446689974375744	12.275664667651816	25.460219221557523
2	16.314208781681046	19.47093913780706	38.450278114227345	25.764573966284555
3	19.8163455711712	22.9792034760678	28.505595369603004	28.698855583157997
4	13.498355723331521	15.041325084053184	35.826613063921705	35.633706128693596
5	14.739207149839773	36.36202358876485	33.940482995973845	14.958286265421533
6	34.49602318539631	34.22094186584804	16.872056222133427	14.410978726622226
7	29.949410373550663	30.944056301345285	20.545531685651913	18.561001639452144
8	27.25742081975967	33.77639622337285	19.92646771820023	19.03971523866725
9	26.987528408150485	15.570261565453073	19.09955409267402	38.34265593372242
10-11	25.979343419482454	24.897881826949796	27.34813561538587	21.77463913818188
12-13	26.680992917047863	22.135119455466253	27.20225740618169	23.981630221304194
14-15	23.533647118973132	23.798110644943005	24.575940337747838	28.092301898336025
16-17	23.904618694146603	26.823782733975694	25.433690023323518	23.837908548554186
18-19	23.95574728656088	26.090654632965926	26.693654731697762	23.259943348775426
20-21	24.522784279076987	25.402916213488925	26.49902950006907	23.575270007365024
22-23	24.159773633122725	25.216634422872563	26.43898733384952	24.184604610155194
24-25	24.017053882675118	25.4204761301566	26.409840150009718	24.152629837158567
26-27	23.797781123033086	25.62806270898402	26.37366974382213	24.200486424160765
28-29	23.879591817332113	25.88445933257159	26.23611568726332	23.999833162832974
30-31	24.089451192809328	25.647404147753548	26.076355907496403	24.186788751940718
32-33	23.74232249878803	25.72357357688783	26.017184604203507	24.516919320120635
34-35	24.23474497992174	25.633282860022167	26.06491681130329	24.06705534875281
36-37	24.364285516950552	25.40966155582297	26.065806096594198	24.16024683063228
38-39	24.06920497349282	25.50772620926336	26.09952467442051	24.323544142823312
40-41	24.308990907895332	25.379251776282135	26.26901751452631	24.042739801296225
42-43	24.191664103892556	25.363634302036292	26.070693161902987	24.374008432168168
44-45	24.026667481884818	25.460104133176554	26.208131760044367	24.305096624894258
46-47	24.162331765697456	25.49233628354171	26.031482111429487	24.313849839331343
48-49	23.991178191122987	25.546011235384558	25.93036777744145	24.53244279605101
50-51	23.92747252792339	25.706226279197576	26.101031924781886	24.265269268097146
52-53	24.122155298740715	25.397994922956574	26.172452390145356	24.307397388157355
54-55	23.68779869992246	25.36976054741403	26.458697366847765	24.48374338581574
56-57	23.6684082831749	25.52096126888675	26.327620905061895	24.483009542876456
58-59	23.69704497881122	25.207261019416862	26.318066414625445	24.777627587146473
60-61	23.520669336480115	25.20842633919983	26.659199862429123	24.61170446189093
62-63	23.571851586043948	25.227083668656498	26.60778930451693	24.593275440782623
64-65	23.399245071090952	25.190061327453954	26.463339311376256	24.947354290078835
66-67	23.443877317950328	25.16139768045595	26.466714798449615	24.928010203144115
68-69	23.693692584544483	24.9364518505295	26.448116024106028	24.92173954081999
70-71	23.45810887170794	24.923336932923533	26.447891573369596	25.17066262199893
72-73	23.515321255674316	24.96771852377909	26.450251663753395	25.0667085567932
74-75	23.53035012589991	25.0839512139261	26.407076681097752	24.978621979076244
76-77	23.572190134073104	24.789920015418716	26.596123997629782	25.0417658528784
78-79	23.506064399193395	24.91618714292012	26.50396177342285	25.073786684463634
80-81	23.683408606578222	24.85089581450135	26.490990588246877	24.974704990673555
82-83	23.623175463406636	24.832398903886215	26.5188319069712	25.025593725735945
84-85	23.527753797191913	24.79757537479776	26.564348740831274	25.110322087179053
86-87	23.655996872072567	24.793981405070088	26.496032374951074	25.05398934790627
88-89	23.669087886761396	24.585537332818074	26.411431424335436	25.333943356085094
90-91	23.63666863605239	24.609781701692818	26.483186360831525	25.27036330142327
92-93	23.85350081093276	24.338214992377676	26.610077547912393	25.198206648777173
94-95	23.730976854369633	24.22389913272584	26.686828965792174	25.358295047112357
96-97	23.9732192110337	23.957931312528117	26.65920351996942	25.40964595646876
98	23.9885634089785	23.9365397565695	26.559827815449527	25.515069019002475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	65.5
2	126.5
3	208.0
4	227.0
5	260.5
6	410.0
7	725.0
8	1126.5
9	1342.0
10	1388.0
11	1524.5
12	1719.0
13	1915.0
14	2168.5
15	2398.5
16	2532.0
17	2677.5
18	2804.0
19	2834.0
20	2904.5
21	3024.5
22	3272.5
23	3806.5
24	4576.0
25	5706.5
26	7347.5
27	9716.0
28	13089.5
29	17665.5
30	24477.0
31	34956.0
32	44300.0
33	52162.5
34	61750.5
35	73661.0
36	86856.0
37	99312.5
38	110721.5
39	122211.0
40	134892.5
41	146132.5
42	158051.0
43	170596.0
44	182012.5
45	194087.5
46	205522.5
47	213827.0
48	218783.0
49	220517.0
50	220490.0
51	219963.0
52	217822.5
53	216800.5
54	212730.5
55	203460.5
56	188625.5
57	170531.5
58	156520.0
59	137381.0
60	113701.0
61	93170.5
62	75891.5
63	58760.0
64	43220.5
65	32889.5
66	24769.5
67	18330.0
68	13655.5
69	10582.0
70	8033.5
71	5993.0
72	4842.0
73	3992.0
74	3145.0
75	2338.0
76	1619.5
77	1021.5
78	603.0
79	349.0
80	227.5
81	140.0
82	90.5
83	61.0
84	37.0
85	24.5
86	17.5
87	18.5
88	17.0
89	11.5
90	7.0
91	6.5
92	4.5
93	4.0
94	4.0
95	1.5
96	2.0
97	4.0
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3836249719545502
2	0.025501436294837473
3	0.019555187928012387
4	0.016530979136637432
5	0.02225245522842789
6	0.022620264405757276
7	0.015202779329614646
8	0.009828678571968608
9	0.0
10-11	4.0867686369931845E-5
12-13	0.005333233071276106
14-15	0.003300065674371996
16-17	0.005792994542937839
18-19	0.03684221926249356
20-21	0.07839443937912176
22-23	0.09782702424802436
24-25	0.06590936119310759
26-27	0.07490025219449259
28-29	0.05814450078282053
30-31	0.053454933771870855
32-33	0.01990256326215681
34-35	0.3407752027956767
36-37	0.5190707015060968
38-39	0.4736667019491026
40-41	0.4828006298527823
42-43	0.43304422169739026
44-45	0.40644957479215715
46-47	0.40532571341698403
48-49	0.39501683953016875
50-51	0.39161460463987185
52-53	0.45177183897641154
54-55	0.4538254402165006
56-57	0.42467656291314676
58-59	0.4094124820539773
60-61	0.4233381461845315
62-63	0.40484551810213737
64-65	0.452098780467371
66-67	0.4737893050082123
68-69	0.4162782533641257
70-71	0.37367369032347186
72-73	0.35950282007469797
74-75	0.34233839179932657
76-77	0.34026435671605254
78-79	0.33934483377272906
80-81	0.3552627976138175
82-83	0.3506140982892378
84-85	0.3517073088996335
86-87	0.33697450796327305
88-89	0.38035555704495566
90-91	0.35826657256200756
92-93	0.34840724322526145
94-95	0.3558349452229966
96-97	0.3562844897730658
98	0.3517481765860034
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4893842.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.97226627717831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.43597961969002	38.166342871199696
2	12.530612277829928	13.024886358389137
3	5.259864776664521	8.201012782642785
4	2.7997572058793803	5.820389080616476
5	1.6504286681189835	4.288825910548427
6	1.038499808898759	3.238391315813106
7	0.690812914010382	2.513217890026274
8	0.50935245503708	2.1177761137697293
9	0.35324435763996126	1.6523018834557388
>10	1.6317846667542868	14.390302373491409
>50	0.06801276825662822	2.4010481410209903
>100	0.029005484894108705	2.6564489359412633
>500	0.0015001492651928734	0.5158894014030873
>1k	0.0011448470607098816	1.013166941682037
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.0867686369931845E-5	0.0
4	0.0	0.0	0.0	6.130152955489776E-5	0.0
5	0.0	0.0	0.0	6.130152955489776E-5	0.0
6	0.0	0.0	0.0	6.130152955489776E-5	2.0433843184965922E-5
7	0.0	0.0	0.0	6.130152955489776E-5	2.0433843184965922E-5
8	0.0	0.0	0.0	6.130152955489776E-5	2.0433843184965922E-5
9	0.0	0.0	0.0	8.173537273986369E-5	4.0867686369931845E-5
10-11	0.0	0.0	0.0	1.0216921592482962E-4	4.0867686369931845E-5
12-13	1.0216921592482961E-5	0.0	0.0	1.2260305910979553E-4	1.328199807022785E-4
14-15	2.0433843184965922E-5	0.0	0.0	1.532538238872444E-4	2.452061182195911E-4
16-17	2.0433843184965922E-5	0.0	0.0	1.6347074547972738E-4	3.2694149095945476E-4
18-19	2.0433843184965922E-5	0.0	0.0	1.839045886646933E-4	3.2694149095945476E-4
20-21	2.0433843184965922E-5	0.0	0.0	2.2477227503462516E-4	3.2694149095945476E-4
22-23	2.0433843184965922E-5	0.0	0.0	3.678091773293866E-4	3.473753341444207E-4
24-25	2.0433843184965922E-5	0.0	0.0	7.254014330662903E-4	3.678091773293866E-4
26-27	2.0433843184965922E-5	0.0	0.0	0.001563189003649893	4.291107068842844E-4
28-29	4.0867686369931845E-5	0.0	0.0	0.006998591290850828	4.495445500692503E-4
30-31	4.0867686369931845E-5	0.0	0.0	0.03164180617191973	4.5976147166173326E-4
32-33	4.0867686369931845E-5	0.0	0.0	0.08160255275916141	4.699783932542162E-4
34-35	4.0867686369931845E-5	0.0	0.0	0.14832926767966764	5.31279922809114E-4
36-37	4.0867686369931845E-5	0.0	0.0	0.25659798579520954	5.31279922809114E-4
38-39	4.0867686369931845E-5	0.0	0.0	0.439848691477984	5.925814523640117E-4
40-41	4.0867686369931845E-5	0.0	0.0	0.6503990116558729	5.925814523640117E-4
42-43	4.0867686369931845E-5	0.0	0.0	0.8692148214020804	6.130152955489776E-4
44-45	4.0867686369931845E-5	0.0	0.0	1.1331281230575079	6.538829819189095E-4
46-47	4.0867686369931845E-5	0.0	0.0	1.4467263144171798	6.845337466963584E-4
48-49	4.0867686369931845E-5	0.0	0.0	1.784252536146447	7.049675898813243E-4
50-51	4.0867686369931845E-5	0.0	0.0	2.156107614426457	7.76486041028705E-4
52-53	4.0867686369931845E-5	0.0	0.0	2.5276868358234696	8.377875705836028E-4
54-55	4.0867686369931845E-5	0.0	0.0	2.943260121597714	8.786552569535346E-4
56-57	4.0867686369931845E-5	0.0	0.0	3.426551163686936	8.888721785460176E-4
58-59	4.0867686369931845E-5	0.0	0.0	3.9423831010482155	8.990891001385006E-4
60-61	4.0867686369931845E-5	0.0	0.0	4.499379015505609	9.399567865084324E-4
62-63	4.0867686369931845E-5	0.0	0.0	5.054730005586613	0.0010012583160633301
64-65	4.0867686369931845E-5	0.0	0.0	5.64704581798922	0.001011475237655813
66-67	4.0867686369931845E-5	0.0	0.0	6.330946115546844	0.001042126002433262
68-69	4.0867686369931845E-5	0.0	0.0	7.069169785211701	0.001062559845618228
70-71	6.130152955489776E-5	0.0	0.0	7.841895999094373	0.001062559845618228
72-73	6.130152955489776E-5	0.0	0.0	8.629998679973731	0.0011340782967656088
74-75	6.130152955489776E-5	0.0	0.0	9.434530170773801	0.0011647290615430575
76-77	6.130152955489776E-5	0.0	1.0216921592482961E-5	10.305267313493161	0.0011749459831355406
78-79	7.151845114738073E-5	0.0	2.0433843184965922E-5	11.255011910887193	0.0012055967479129893
80-81	8.173537273986369E-5	0.0	2.0433843184965922E-5	12.227356338843796	0.0012464644342829213
82-83	8.173537273986369E-5	0.0	2.0433843184965922E-5	13.221779534361755	0.001307765963837819
84-85	8.173537273986369E-5	2.0433843184965922E-5	2.0433843184965922E-5	14.22543882699932	0.001307765963837819
86	1.0216921592482962E-4	2.0433843184965922E-5	2.0433843184965922E-5	15.010353828341822	0.001307765963837819
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6780	0.0	43.918682	1
GGTATCA	3700	0.0	38.932953	1
TCAACGC	8275	0.0	35.30808	4
ATCAACG	8390	0.0	34.76945	3
CAACGCA	8505	0.0	34.46111	5
AACGCAG	8995	0.0	32.782787	6
TATCAAC	9005	0.0	32.44712	2
ACGCAGA	10900	0.0	26.627956	7
GTACATG	13915	0.0	25.831078	1
CGCAGAG	11305	0.0	25.509691	8
TACATGG	14550	0.0	24.08523	2
ACATGGG	14925	0.0	23.171795	3
GCAGAGT	12815	0.0	21.6101	9
CATGGGG	10410	0.0	20.047646	4
GAGTACA	10380	0.0	19.065395	1
TAAGGCG	1860	0.0	18.988054	5
AGAGTAC	11025	0.0	18.0702	10-11
AGTACAT	10620	0.0	17.276506	2
CAGAGTA	12030	0.0	16.751163	10-11
GTATTAA	3305	0.0	16.571058	1
>>END_MODULE