FastQCFastQC Report
Mon 15 Aug 2016
SRR2920898_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920898_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4893842
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69700.041.9658131
GGTATCA39500.041.661251
TCAACGC85800.033.695294
CAACGCA89000.032.4797825
ATCAACG89450.032.3734253
TATCAAC93450.030.9855042
AACGCAG94800.030.5930756
GTACATG140150.025.4519731
ACGCAGA113050.025.1502387
CGCAGAG115050.024.668248
TACATGG146350.023.9695422
ACATGGG148500.022.6012333
CATGGGG101900.019.892664
GCAGAGT135050.019.890499
TACACCG5100.019.5449685
GAGTACA105050.019.3776451
TAAGGTG22800.018.9449025
GTATAGG10700.018.6689011
AGAGTAC112150.018.13193110-11
AGTACAT109200.016.9966052