##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920898_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4893842
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.234619957080756	31.0	30.0	34.0	26.0	34.0
2	30.25051094824884	31.0	30.0	34.0	26.0	34.0
3	30.392588072929204	31.0	30.0	34.0	27.0	34.0
4	34.03475817159606	35.0	35.0	37.0	30.0	37.0
5	33.55306260398272	35.0	35.0	37.0	28.0	37.0
6	33.489831506615864	35.0	35.0	37.0	28.0	37.0
7	33.300409371614364	35.0	33.0	37.0	27.0	37.0
8	33.23040711163131	35.0	33.0	37.0	27.0	37.0
9	34.55942570275052	38.0	34.0	39.0	27.0	39.0
10-11	34.37304861906044	37.5	34.0	39.0	27.0	39.0
12-13	34.189627903802375	37.0	34.0	39.0	26.0	39.0
14-15	35.090623685848456	38.0	34.0	40.0	26.0	41.0
16-17	34.966283750067944	38.0	34.0	40.0	25.0	41.0
18-19	34.87059829475492	38.0	34.0	40.0	25.0	41.0
20-21	34.74910448682242	38.0	34.0	40.0	25.0	41.0
22-23	34.602630918611595	38.0	33.5	40.0	24.0	41.0
24-25	34.42387933652129	38.0	33.0	40.0	24.0	41.0
26-27	34.240421125160964	38.0	33.0	40.0	23.0	41.0
28-29	34.18090776122319	38.0	33.0	40.0	22.5	41.0
30-31	34.16744553665607	38.0	33.0	40.0	22.5	41.0
32-33	34.021434692824165	38.0	33.0	40.0	22.0	41.0
34-35	33.85800706275356	38.0	33.0	40.0	20.5	41.0
36-37	33.66305440592484	38.0	32.5	40.0	19.0	41.0
38-39	33.479008312896084	38.0	32.0	40.0	18.0	41.0
40-41	33.24481215372299	38.0	32.0	40.0	16.5	41.0
42-43	33.27815650770907	38.0	32.0	40.0	16.0	41.0
44-45	33.13752507743405	38.0	32.0	40.0	15.0	41.0
46-47	32.89243829285866	37.5	31.5	40.0	14.0	41.0
48-49	32.626844409770484	37.0	31.0	40.0	10.5	41.0
50-51	32.367251129072	37.0	31.0	40.0	9.0	41.0
52-53	32.10816430117687	37.0	31.0	40.0	8.0	41.0
54-55	31.820816139957113	36.0	30.0	40.0	7.0	41.0
56-57	31.488844654159248	36.0	30.0	39.0	4.0	41.0
58-59	31.153148283087194	35.5	29.5	39.0	2.0	40.0
60-61	30.824916497099824	35.0	29.0	39.0	2.0	40.0
62-63	30.507346375301857	35.0	29.0	38.5	2.0	40.0
64-65	30.1022787413243	35.0	28.0	38.0	2.0	40.0
66-67	29.670988152049045	34.0	28.0	38.0	2.0	40.0
68-69	29.231436670820187	34.0	28.0	37.0	2.0	39.5
70-71	28.79995349257291	34.0	26.5	36.5	2.0	39.0
72-73	28.363432758965246	34.0	26.0	36.0	2.0	39.0
74-75	27.922187516474786	33.0	26.0	35.5	2.0	38.5
76-77	26.474560069573148	31.5	24.5	34.0	2.0	36.0
78-79	27.32640408088369	33.0	26.0	35.0	2.0	37.0
80-81	27.35491644397183	33.0	26.0	35.0	2.0	36.5
82-83	27.02236116327417	33.0	25.5	35.0	2.0	36.0
84-85	26.712607599509752	33.0	25.0	35.0	2.0	36.0
86-87	26.184876115738923	32.5	24.0	35.0	2.0	35.0
88-89	25.82166639217204	32.0	23.0	35.0	2.0	35.0
90-91	25.457658420521135	32.0	20.5	34.5	2.0	35.0
92-93	25.1539207845288	32.0	19.5	34.0	2.0	35.0
94-95	24.77596691924259	32.0	17.5	34.0	2.0	35.0
96-97	24.251723288165003	32.0	6.0	34.0	2.0	35.0
98-99	23.65660967395351	31.0	2.0	34.0	2.0	35.0
100-101	21.46257602922203	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	116212.0
3	53016.0
4	28186.0
5	20175.0
6	17387.0
7	17056.0
8	19230.0
9	22530.0
10	25486.0
11	28268.0
12	31363.0
13	34264.0
14	36538.0
15	39085.0
16	41147.0
17	42176.0
18	41460.0
19	40293.0
20	40370.0
21	42198.0
22	45847.0
23	51513.0
24	60922.0
25	71676.0
26	84876.0
27	100630.0
28	118694.0
29	140271.0
30	165005.0
31	194907.0
32	233922.0
33	285881.0
34	359257.0
35	467075.0
36	619413.0
37	718283.0
38	410256.0
39	28974.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.89914437804188	18.7090146272395	12.933499873170673	25.458341121547946
2	16.36470895464136	20.459201584358468	37.33823037196542	25.837859089034747
3	19.61411347709049	23.77557910363088	28.82171202300638	27.788595396272253
4	13.96144059370834	15.943711008205916	35.35982859397892	34.73501980410682
5	14.705192361452834	36.58864746185549	34.00826321304851	14.69789696364317
6	34.46416162895569	34.70883145356295	16.628302127242627	14.198704790238736
7	29.822534487856732	31.106036282974873	20.557187481135657	18.514241748032738
8	27.242282125391565	34.064115445528714	19.804831521569007	18.888770907510715
9	26.8355189841195	15.523256789514114	19.044403436634543	38.59682078973184
10-11	25.97999243901501	25.067713366659493	27.348845763270795	21.6034484310547
12-13	26.629047571411125	22.190571229667142	27.2664793997147	23.91390179920703
14-15	23.494072153770492	23.90376987780159	24.540405751418337	28.061752217009577
16-17	23.892834329394617	26.959045482391105	25.40129055845136	23.746829629762917
18-19	23.962409404683672	26.135030311681323	26.66266389622054	23.239896387414465
20-21	24.517072829709967	25.467442838798192	26.446604505848477	23.56887982564336
22-23	24.153992488749896	25.266446095614235	26.47015214739785	24.109409268238014
24-25	23.982455213124528	25.539848442826564	26.420563540304588	24.057132803744327
26-27	23.792899151179938	25.740865791350267	26.340678187036897	24.125556870432895
28-29	23.864133591624594	25.90702352780166	26.27814771519453	23.950695165379212
30-31	24.070072978975368	25.697659873745103	26.09468463051251	24.137582516767015
32-33	23.752138182083534	25.713792811293096	26.047204601272796	24.486864405350573
34-35	24.17981101729114	25.703882262425527	26.071702329189865	24.04460439109347
36-37	24.30029833376408	25.493490319047385	26.095574107780926	24.11063723940761
38-39	24.05537287005312	25.513106087898556	26.135422335666085	24.29609870638224
40-41	24.226765563715222	25.425895683916423	26.307047538849247	24.04029121351911
42-43	24.178062189276883	25.387510440460204	26.115220990337047	24.31920637992587
44-45	23.931532653302327	25.489471483214587	26.297602749665717	24.281393113817373
46-47	24.09110285921087	25.50825481303784	26.086715677271595	24.313926650479697
48-49	23.917734342989508	25.558398378228702	25.999245925391573	24.52462135339022
50-51	23.85969085735552	25.69345367468881	26.132604385662123	24.31425108229355
52-53	24.105624086036975	25.398373442124733	26.16554041627261	24.330462055565683
54-55	23.685413042625267	25.374291268294595	26.369550449926106	24.570745239154036
56-57	23.639886982801894	25.546656274844352	26.18421459984962	24.62924214250413
58-59	23.62687214056219	25.27229602855775	26.177057962511924	24.923773868368134
60-61	23.447651503817486	25.378049308895534	26.436340069016428	24.73795911827055
62-63	23.462056670121022	25.405341998721937	26.30184677654533	24.83075455461171
64-65	23.22460057568067	25.32975553444597	26.231214537863046	25.21442935201031
66-67	23.27103988225434	25.365727018132013	26.181381467323533	25.181851632290115
68-69	23.48964483910856	25.156346956280935	26.12948379018809	25.22452441442241
70-71	23.24371879144173	25.07405541850011	26.12050367597128	25.561722114086887
72-73	23.271780545014987	25.094282498330838	26.100470145033956	25.533466811620215
74-75	23.163249925709685	25.213877369544058	25.964705504600477	25.658167200145776
76-77	23.147110532755047	24.87001071870642	26.267273223565173	25.715605524973356
78-79	22.988759260940732	25.0037628642923	26.181989295469105	25.82548857929787
80-81	23.084215243016722	24.98029083220971	26.125297681241317	25.810196243532253
82-83	22.96196764227584	24.95266199936954	26.129002757359988	25.956367600994636
84-85	22.69448287919585	24.86421244443963	26.155592843026366	26.285711833338155
86-87	22.751137707473344	24.858088766178472	26.088331009976095	26.30244251637209
88-89	22.67168889412509	24.70205583172392	26.007750670853707	26.618504603297282
90-91	22.468977664574112	24.721420539355773	26.064344769082044	26.745257026988075
92-93	22.55676863618398	24.504078319315152	26.143157184593484	26.795995859907386
94-95	22.234707431795457	24.34681255766874	26.303107989604378	27.11537202093142
96-97	22.342993675965655	24.129345634352394	26.327334551583103	27.200326138098845
98-99	22.11957751695424	24.207389621834253	26.196625755118202	27.47640710609331
100-101	22.29490455224775	23.934711781842935	26.335156363110258	27.43522730279906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	53.0
2	93.0
3	163.0
4	150.5
5	136.0
6	246.0
7	493.5
8	742.5
9	779.0
10	653.5
11	595.0
12	593.5
13	618.0
14	711.5
15	816.5
16	903.0
17	1007.5
18	1150.0
19	1301.5
20	1516.0
21	1810.5
22	2270.0
23	2890.5
24	3483.0
25	4361.5
26	5730.5
27	7730.5
28	10416.0
29	13968.0
30	19355.0
31	27042.5
32	37819.5
33	46614.0
34	53350.0
35	63229.0
36	75216.5
37	87743.0
38	99468.5
39	110136.0
40	121546.0
41	133350.0
42	144128.5
43	156816.5
44	169283.5
45	181173.0
46	193364.5
47	202820.0
48	211249.5
49	217684.5
50	219152.0
51	218875.0
52	217317.5
53	217337.0
54	214844.5
55	204304.5
56	189707.5
57	172364.5
58	156822.0
59	138572.5
60	115896.0
61	95340.5
62	77726.0
63	59822.5
64	43961.5
65	33319.0
66	25098.0
67	18702.0
68	13985.0
69	10530.5
70	8234.0
71	6299.5
72	4770.0
73	4115.0
74	3312.5
75	2376.5
76	1691.0
77	1106.0
78	636.5
79	354.0
80	202.5
81	117.5
82	67.5
83	35.0
84	18.0
85	9.5
86	4.5
87	4.0
88	4.0
89	3.0
90	1.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19054558769980723
2	0.0
3	0.012055967479129895
4	0.013588505718002339
5	0.007070109741998209
6	0.10652162452322736
7	0.14256692390150724
8	0.12393125891681832
9	0.14238301931284253
10-11	0.11422518340395951
12-13	0.11510383866091305
14-15	0.11700418607711487
16-17	0.06632825497839939
18-19	0.07960003612703476
20-21	0.036780917732938664
22-23	0.08398309549020994
24-25	0.05755191933045652
26-27	0.06599109656584745
28-29	0.0398153434459061
30-31	0.024520611821959106
32-33	0.031539636955994904
34-35	0.019626706379159767
36-37	0.023856511918447714
38-39	0.03176440923102953
40-41	0.024765817940178698
42-43	0.012648548931493907
44-45	0.02697267300415502
46-47	0.033419550529011764
48-49	0.00872525103998045
50-51	0.015550154663759067
52-53	0.006497962132819163
54-55	0.012137702851869757
56-57	0.005118677717833964
58-59	0.0036576579301089004
60-61	0.022354624444352718
62-63	0.010319090808407792
64-65	0.006753385172631237
66-67	0.03968252346520382
68-69	0.08982717464111019
70-71	0.0215270537953616
72-73	0.04413710127952639
74-75	0.08665992894744047
76-77	0.2490783315031421
78-79	0.0807136805806154
80-81	0.016643365274154744
82-83	0.011739242909762923
84-85	0.012842670441751082
86-87	0.2651086814817479
88-89	0.6183893963066237
90-91	0.26489412612830576
92-93	0.35022585526872346
94-95	0.001052342924025745
96-97	0.00428089014725036
98-99	0.0031570287720772347
100-101	0.005077810031464031
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4893842.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.04531553801091
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.81803375051848	34.73292811075686
2	13.507038261468104	13.249139070353788
3	5.86059046654142	8.623035260117474
4	3.113553452970664	6.108208461816385
5	1.8248833525658246	4.475098992332704
6	1.1601506087196127	3.41399716057613
7	0.7898510371063996	2.711694534003597
8	0.5518895539242568	2.165407785147781
9	0.3908588157553285	1.7252814554580018
>10	1.8656678495140997	15.513485056285187
>50	0.08094371527118267	2.7198198519063856
>100	0.033700975226362805	2.9639067802050643
>500	0.0015025570537825978	0.5016879446152547
>1k	0.0013356033644166885	1.0963095364254167
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0867686369931845E-5
2	0.0	0.0	0.0	0.0	4.0867686369931845E-5
3	0.0	0.0	0.0	0.0	4.0867686369931845E-5
4	0.0	0.0	0.0	0.0	4.0867686369931845E-5
5	0.0	0.0	0.0	0.0	4.0867686369931845E-5
6	0.0	0.0	0.0	0.0	8.173537273986369E-5
7	0.0	0.0	0.0	0.0	8.173537273986369E-5
8	0.0	0.0	0.0	0.0	8.173537273986369E-5
9	0.0	0.0	0.0	8.173537273986369E-5	8.173537273986369E-5
10-11	0.0	0.0	0.0	1.0216921592482962E-4	8.173537273986369E-5
12-13	0.0	0.0	0.0	1.0216921592482962E-4	8.173537273986369E-5
14-15	0.0	0.0	0.0	1.328199807022785E-4	1.1238613751731258E-4
16-17	0.0	0.0	0.0	1.4303690229476145E-4	1.6347074547972738E-4
18-19	0.0	0.0	0.0	1.6347074547972738E-4	2.2477227503462516E-4
20-21	0.0	0.0	0.0	2.145553534421422E-4	2.349891966271081E-4
22-23	0.0	1.0216921592482961E-5	0.0	4.0867686369931846E-4	2.860738045895229E-4
24-25	0.0	2.0433843184965922E-5	0.0	7.969198842136709E-4	3.473753341444207E-4
26-27	0.0	2.0433843184965922E-5	0.0	0.001614273611612308	3.882430205143525E-4
28-29	0.0	2.0433843184965922E-5	0.0	0.007080326663590692	4.188937852918014E-4
30-31	0.0	2.0433843184965922E-5	0.0	0.03203004919243409	4.2911070688428436E-4
32-33	0.0	2.0433843184965922E-5	0.0	0.08219513421152543	4.3932762847676736E-4
34-35	0.0	2.0433843184965922E-5	0.0	0.1486459922490346	4.699783932542162E-4
36-37	0.0	2.0433843184965922E-5	0.0	0.25672058885431936	4.699783932542162E-4
38-39	0.0	2.0433843184965922E-5	0.0	0.4411564574418218	4.8019531484669916E-4
40-41	0.0	2.0433843184965922E-5	0.0	0.6547718540974555	4.904122364391821E-4
42-43	0.0	2.0433843184965922E-5	0.0	0.8763462326736335	4.904122364391821E-4
44-45	0.0	2.0433843184965922E-5	0.0	1.1433859123363606	5.006291580316651E-4
46-47	0.0	2.0433843184965922E-5	0.0	1.460692846234104	5.10846079624148E-4
48-49	0.0	2.0433843184965922E-5	0.0	1.7978614757076343	5.31279922809114E-4
50-51	0.0	2.0433843184965922E-5	0.0	2.166518657529197	5.31279922809114E-4
52-53	0.0	2.0433843184965922E-5	0.0	2.5348284640166154	5.41496844401597E-4
54-55	0.0	2.0433843184965922E-5	0.0	2.9472958056267444	5.619306875865629E-4
56-57	0.0	2.0433843184965922E-5	0.0	3.426837237491525	5.721476091790458E-4
58-59	0.0	2.0433843184965922E-5	0.0	3.9389808661579186	5.721476091790458E-4
60-61	0.0	2.0433843184965922E-5	0.0	4.492870836451197	6.027983739564948E-4
62-63	0.0	2.0433843184965922E-5	0.0	5.045493908467009	6.538829819189095E-4
64-65	2.0433843184965922E-5	2.0433843184965922E-5	0.0	5.635439395060159	6.538829819189095E-4
66-67	2.0433843184965922E-5	2.0433843184965922E-5	0.0	6.312964333544074	6.640999035113925E-4
68-69	2.0433843184965922E-5	2.0433843184965922E-5	0.0	7.04435288266356	6.743168251038754E-4
70-71	2.0433843184965922E-5	2.0433843184965922E-5	0.0	7.811766297318139	6.743168251038754E-4
72-73	2.0433843184965922E-5	2.0433843184965922E-5	0.0	8.597468001623264	7.662691194362222E-4
74-75	2.0433843184965922E-5	2.0433843184965922E-5	0.0	9.403399210681506	8.07136805806154E-4
76-77	2.0433843184965922E-5	2.0433843184965922E-5	0.0	10.276108219268215	8.173537273986369E-4
78-79	2.0433843184965922E-5	2.0433843184965922E-5	0.0	11.22429984458019	8.173537273986369E-4
80-81	2.0433843184965922E-5	2.0433843184965922E-5	0.0	12.195101517376328	8.275706489911199E-4
82-83	2.0433843184965922E-5	2.0433843184965922E-5	0.0	13.187634582399678	8.582214137685687E-4
84-85	2.0433843184965922E-5	2.0433843184965922E-5	0.0	14.191886456489605	8.582214137685687E-4
86-87	2.0433843184965922E-5	2.0433843184965922E-5	0.0	15.245659749538298	8.786552569535347E-4
88-89	2.0433843184965922E-5	2.0433843184965922E-5	0.0	16.347033680286366	8.990891001385006E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6970	0.0	41.965813	1
GGTATCA	3950	0.0	41.66125	1
TCAACGC	8580	0.0	33.69529	4
CAACGCA	8900	0.0	32.479782	5
ATCAACG	8945	0.0	32.373425	3
TATCAAC	9345	0.0	30.985504	2
AACGCAG	9480	0.0	30.593075	6
GTACATG	14015	0.0	25.451973	1
ACGCAGA	11305	0.0	25.150238	7
CGCAGAG	11505	0.0	24.66824	8
TACATGG	14635	0.0	23.969542	2
ACATGGG	14850	0.0	22.601233	3
CATGGGG	10190	0.0	19.89266	4
GCAGAGT	13505	0.0	19.89049	9
TACACCG	510	0.0	19.544968	5
GAGTACA	10505	0.0	19.377645	1
TAAGGTG	2280	0.0	18.944902	5
GTATAGG	1070	0.0	18.668901	1
AGAGTAC	11215	0.0	18.131931	10-11
AGTACAT	10920	0.0	16.996605	2
>>END_MODULE