FastQCFastQC Report
Mon 15 Aug 2016
SRR2920897_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920897_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4356038
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81750.033.5529171
GGTATCA52100.029.6806491
TCAACGC96950.027.9117584
CAACGCA96450.027.7235815
ATCAACG97900.027.5472093
TATCAAC100850.027.0616342
AACGCAG102250.026.5519546
GTACATG131200.026.2034571
TACATGG135350.024.670962
ACATGGG137250.023.7596633
ACGCAGA117950.022.8595477
CGCAGAG122650.021.7201048
GAGTACA95100.019.841531
CATGGGG94300.019.6009164
AGTACAT97400.018.1307052
GCAGAGT139100.017.8986849
AGAGTAC103500.017.7210910-11
GTATTAG12500.017.3045231
GAGTACT48700.016.1520712-13
GTGTAGC23900.015.9827681
Produced by FastQC (version 0.11.5)