##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920897_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4356038
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.401485248751275	31.0	28.0	33.0	18.0	34.0
2	28.418028951997204	31.0	28.0	33.0	16.0	34.0
3	28.496690570651587	31.0	28.0	34.0	16.0	34.0
4	31.960424817230702	35.0	32.0	37.0	20.0	37.0
5	31.91551106762613	35.0	32.0	37.0	20.0	37.0
6	32.0417262200192	35.0	32.0	37.0	22.0	37.0
7	31.87132458440445	35.0	32.0	37.0	19.0	37.0
8	31.813576695152797	35.0	32.0	37.0	19.0	37.0
9	33.062204461944546	37.0	32.0	39.0	18.0	39.0
10-11	32.86157570250765	37.0	32.0	39.0	17.5	39.0
12-13	32.68883237474053	37.0	32.0	39.0	17.0	39.0
14-15	33.427694156938024	38.0	32.0	40.0	17.0	41.0
16-17	33.32005574790669	38.0	32.0	40.0	17.0	41.0
18-19	33.20272320856705	38.0	32.0	40.0	16.0	41.0
20-21	33.10159622115326	38.0	32.0	40.0	12.5	41.0
22-23	32.87744562834392	38.0	32.0	40.0	10.5	41.0
24-25	32.68314693306165	38.0	31.0	40.0	10.0	41.0
26-27	32.710741044958745	38.0	31.5	40.0	10.0	41.0
28-29	32.550317168950315	38.0	31.0	40.0	9.0	41.0
30-31	32.30703497076931	37.5	31.0	40.0	8.5	41.0
32-33	32.055529015127966	37.0	30.0	40.0	8.0	41.0
34-35	31.72870519035876	37.0	30.0	40.0	4.0	41.0
36-37	31.419263330577007	37.0	30.0	40.0	2.0	41.0
38-39	31.211516061154654	37.0	30.0	40.0	2.0	41.0
40-41	31.013023302367884	36.0	29.5	40.0	2.0	41.0
42-43	30.77559929458834	36.0	29.0	39.5	2.0	41.0
44-45	30.49310703901114	36.0	28.0	39.0	2.0	40.0
46-47	30.23981115408084	36.0	28.0	39.0	2.0	40.0
48-49	29.93628223628903	35.0	27.0	39.0	2.0	40.0
50-51	28.807106251139224	34.0	25.5	38.0	2.0	39.5
52-53	29.258494531039446	34.5	26.5	38.5	2.0	39.5
54-55	29.902071676142405	35.0	28.0	39.0	2.0	40.0
56-57	29.849925551613644	35.0	28.0	39.0	2.0	40.0
58-59	29.60711752284989	35.0	27.5	39.0	2.0	40.0
60-61	29.282229746388808	35.0	26.5	39.0	2.0	40.0
62-63	28.894541438802875	34.5	26.0	38.5	2.0	40.0
64-65	28.477009842430206	34.0	26.0	38.0	2.0	40.0
66-67	28.088951703359797	34.0	25.5	37.5	2.0	40.0
68-69	27.644181363890766	34.0	24.0	37.0	2.0	39.5
70-71	27.30601340025041	33.5	24.0	36.5	2.0	39.0
72-73	26.846326868590218	33.0	22.5	36.0	2.0	39.0
74-75	26.401415690129426	33.0	21.5	35.5	2.0	38.5
76-77	25.982996704803767	32.5	20.0	35.0	2.0	37.5
78-79	25.515194885811372	32.0	18.5	35.0	2.0	37.0
80-81	25.05428786433911	32.0	17.0	35.0	2.0	36.0
82-83	24.567782466544138	31.5	10.0	35.0	2.0	36.0
84-85	24.16357880716376	31.0	6.5	34.0	2.0	35.5
86-87	23.69340017235846	31.0	2.0	34.0	2.0	35.0
88-89	23.145099973875343	31.0	2.0	34.0	2.0	35.0
90-91	22.630325998074397	30.0	2.0	34.0	2.0	35.0
92-93	22.078378792838812	30.0	2.0	34.0	2.0	35.0
94-95	21.42504909277651	29.5	2.0	34.0	2.0	35.0
96-97	20.76664666378025	29.0	2.0	34.0	2.0	35.0
98	20.358320106482083	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	240129.0
3	39850.0
4	29135.0
5	23910.0
6	22372.0
7	22717.0
8	25858.0
9	29428.0
10	32275.0
11	34651.0
12	35570.0
13	35233.0
14	33243.0
15	35101.0
16	37402.0
17	40262.0
18	41751.0
19	41083.0
20	42921.0
21	45556.0
22	49869.0
23	55331.0
24	62696.0
25	72503.0
26	83192.0
27	95712.0
28	110518.0
29	128118.0
30	147906.0
31	173070.0
32	205022.0
33	246994.0
34	306441.0
35	385788.0
36	483837.0
37	522574.0
38	307629.0
39	30391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.38313322309296	17.872420734776046	12.702944597760904	25.04150144437009
2	16.19735741732113	19.48646400834722	38.93223898514391	25.383939589187737
3	19.57066958544487	23.2674059588354	28.895628652060378	28.266295803659357
4	13.294923770572165	15.304870642675574	36.37698466181393	35.023220924938336
5	14.438910702394894	36.71422010975638	34.17113269500126	14.675736492847467
6	33.962279314564306	34.700073088219526	17.085490714030342	14.25215688318583
7	29.662957878517155	31.279666229730875	20.65576646183723	18.401609429914743
8	27.01741843174421	34.03856079140126	19.92039702306172	19.023623753792805
9	27.00382779029935	15.497270684966477	19.340097584089026	38.15880394064515
10-11	25.823208113246054	25.075601974914353	27.537541577355746	21.563648334483844
12-13	26.435582123488853	22.414260178511654	27.351695236276097	23.798462461723396
14-15	23.46784456831528	24.109436861402223	24.836224309591525	27.586494260690976
16-17	23.669482931716534	27.01889149768962	25.697138668281077	23.614486902312773
18-19	23.6826648983353	26.268918418491694	26.99669012002262	23.051726563150385
20-21	24.501709331017466	25.53641748112614	26.642049083532065	23.319824104324333
22-23	24.060758121885065	25.422520250351898	26.515342401253573	24.00137922650946
24-25	23.838029746309854	25.576921972504934	26.621591975456827	23.963456305728386
26-27	23.69577396061146	25.793843382485633	26.575645564019272	23.934737092883637
28-29	23.748993620039162	26.01969690879125	26.437409196101964	23.793900275067617
30-31	23.929372879681925	25.772938482351833	26.231076484193938	24.066612153772297
32-33	23.704844195555122	25.779010743780212	26.247373524483887	24.268771536180783
34-35	24.161687384213113	25.773818613844234	26.226082802467083	23.83841119947557
36-37	24.259245264951034	25.514433441072416	26.187138341941115	24.039182952035436
38-39	23.981112686745217	25.69419098415167	26.25513440881605	24.06956192028706
40-41	24.145328815498416	25.58917149704411	26.39096417639854	23.87453551105893
42-43	24.019112568815977	25.51243906574761	26.20839413454255	24.260054230893857
44-45	23.916840991164996	25.55926356850008	26.3531046048404	24.170790835494525
46-47	24.076041066813374	25.55777496871496	26.138474248654763	24.227709715816907
48-49	23.889878592894412	25.617291063625803	26.123084865133944	24.369745478345838
50-51	23.711062948816082	25.788247245183427	26.284587234677037	24.216102571323454
52-53	23.95575586818951	25.529222855671023	26.327854892197706	24.187166383941765
54-55	23.5767373750033	25.48037783416264	26.516669666283843	24.426215124550215
56-57	23.59606664794037	25.59216916553604	26.435716955685184	24.376047230838406
58-59	23.585607139729035	25.33043374038271	26.448759260027565	24.63519985986069
60-61	23.426720970695072	25.295576396304913	26.78717750259485	24.490525130405167
62-63	23.407056633696865	25.369168693835594	26.745526985364627	24.478247687102918
64-65	23.356698440691538	25.344573458043573	26.507690470052342	24.791037631212546
66-67	23.327822622773795	25.364344610993268	26.448239413312287	24.85959335292065
68-69	23.59459006857671	25.12659105375966	26.430056793238705	24.848762084424926
70-71	23.411892977442463	24.99701040792782	26.546264254133067	25.04483236049665
72-73	23.43885834992367	24.974430552066238	26.576824859159252	25.009886238850836
74-75	23.374539247928237	25.18121139284898	26.50509080292509	24.9391585562977
76-77	23.475063901064086	24.888638129373945	26.6478550963186	24.98844287324337
78-79	23.486274730654696	24.968803981205113	26.569299386076175	24.97562190206402
80-81	23.594686892682752	24.889939946719647	26.532132697753326	24.98324046284428
82-83	23.523058510047075	24.84950723792352	26.622755019333727	25.004679232695683
84-85	23.520601830628824	24.78631759986473	26.655992528948254	25.037088040558192
86-87	23.511732701066375	24.798831129166814	26.65044156267721	25.038994607089606
88-89	23.623879689060786	24.58954480149225	26.536901276684976	25.249674232761986
90-91	23.573703394996453	24.603668271483606	26.59539445644717	25.227233877072774
92-93	23.824298935387983	24.40241117943974	26.639268924644195	25.134020960528087
94-95	23.66921607241773	24.296333258117052	26.74647194749466	25.28797872197056
96-97	23.86207049073658	24.057805781246202	26.75704761655741	25.3230761114598
98	23.907459276566893	23.949638002410016	26.696599510118634	25.446303210904457
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	61.0
2	99.0
3	164.0
4	183.0
5	221.5
6	340.0
7	617.5
8	941.5
9	1142.0
10	1241.0
11	1323.5
12	1503.0
13	1724.0
14	1900.5
15	2091.5
16	2294.5
17	2330.5
18	2345.5
19	2446.5
20	2546.0
21	2715.5
22	2902.0
23	3299.0
24	3922.5
25	4925.0
26	6383.0
27	8256.5
28	10911.0
29	14646.5
30	20338.0
31	29159.0
32	37996.0
33	46307.0
34	56697.0
35	69246.0
36	82241.0
37	94961.0
38	107388.0
39	118353.0
40	128476.0
41	138044.5
42	148940.0
43	160845.0
44	170596.5
45	178692.5
46	186730.0
47	192833.5
48	196662.5
49	196887.5
50	195369.0
51	193824.5
52	190612.0
53	188102.5
54	181974.5
55	172034.0
56	158443.0
57	141709.0
58	127974.5
59	112442.5
60	94481.0
61	78500.5
62	64225.0
63	49555.5
64	36567.5
65	27837.0
66	21646.5
67	17091.5
68	13170.5
69	10115.0
70	7677.0
71	5833.5
72	4683.5
73	3799.0
74	2884.0
75	2057.5
76	1473.0
77	966.5
78	583.5
79	325.5
80	180.5
81	115.0
82	71.0
83	48.0
84	37.0
85	20.0
86	14.0
87	10.0
88	8.5
89	9.5
90	9.0
91	4.5
92	3.0
93	4.5
94	3.5
95	2.5
96	3.0
97	2.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3771776095617164
2	0.024242212763065887
3	0.0181587029314253
4	0.016046691971006683
5	0.021533329139920267
6	0.023576470177716538
7	0.0162303450979996
8	0.009687702448876708
9	0.0
10-11	2.2956640874115422E-5
12-13	0.005601420373284163
14-15	0.003592714296799064
16-17	0.006175336395137048
18-19	0.037889935762727506
20-21	0.07938406414269114
22-23	0.09925303681923804
24-25	0.06641356204881592
26-27	0.07517152054229095
28-29	0.05883787056035782
30-31	0.05344305995494071
32-33	0.01990340763785807
34-35	0.33399616807750526
36-37	0.511141087382617
38-39	0.46412588687242856
40-41	0.47354958795125296
42-43	0.4240895051879713
44-45	0.3991241582373708
46-47	0.39723023536525626
48-49	0.3883804503082847
50-51	0.3850861723428492
52-53	0.444727525333801
54-55	0.4447504819746752
56-57	0.4157332879097932
58-59	0.40169530201527165
60-61	0.415871027755038
62-63	0.39644970957553627
64-65	0.444716047013364
66-67	0.464619454651222
68-69	0.4076525503221047
70-71	0.3668700778092386
72-73	0.3537274009088075
74-75	0.3358671343087457
76-77	0.3342716477679947
78-79	0.3337551233483271
80-81	0.34953781394928146
82-83	0.34546301019412595
84-85	0.3447169193657172
86-87	0.3308511082777515
88-89	0.37380298335322143
90-91	0.35252217726291646
92-93	0.3426508216870468
94-95	0.34945746570622205
96-97	0.3495492922697185
98	0.34405117678036784
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4356038.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.03436792810723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23032446310825	41.40253355853585
2	12.104330352714548	13.323083403092967
3	4.921468116778735	8.125496612557496
4	2.532207251206933	5.574337021325737
5	1.439855649440793	3.962077278734419
6	0.9488302887094742	3.1330965126101646
7	0.6233400678785559	2.401358864797185
8	0.422274541043818	1.8591689966782479
9	0.3071042674728367	1.5211160323552753
>10	1.3847260085308641	13.040628348644812
>50	0.05926520879992477	2.2254016332425732
>100	0.024219061575522418	2.3263990151068894
>500	0.001341859990260454	0.49379199671862856
>1k	7.128627496429235E-4	0.611510725599667
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3773984524469255E-4
2	0.0	2.2956640874115422E-5	0.0	0.0	1.3773984524469255E-4
3	0.0	2.2956640874115422E-5	0.0	0.0	1.3773984524469255E-4
4	0.0	4.5913281748230844E-5	0.0	0.0	1.3773984524469255E-4
5	0.0	6.886992262234627E-5	0.0	0.0	1.8365312699292338E-4
6	0.0	6.886992262234627E-5	0.0	0.0	2.0660976786703882E-4
7	0.0	9.182656349646169E-5	0.0	0.0	2.0660976786703882E-4
8	0.0	9.182656349646169E-5	0.0	0.0	2.2956640874115424E-4
9	0.0	9.182656349646169E-5	0.0	2.2956640874115422E-5	2.2956640874115424E-4
10-11	0.0	1.1478320437057712E-4	0.0	2.2956640874115422E-5	2.2956640874115424E-4
12-13	0.0	1.1478320437057712E-4	0.0	3.443496131117314E-5	6.198293036011164E-4
14-15	0.0	1.1478320437057712E-4	0.0	6.886992262234627E-5	6.886992262234627E-4
16-17	0.0	1.1478320437057712E-4	0.0	9.182656349646169E-5	7.805257897199243E-4
18-19	0.0	1.1478320437057712E-4	0.0	1.1478320437057712E-4	8.608740327793284E-4
20-21	0.0	2.2956640874115424E-4	0.0	2.1808808830409653E-4	9.5270059627579E-4
22-23	0.0	2.2956640874115424E-4	0.0	4.017412152970199E-4	9.871355575869632E-4
24-25	0.0	2.4104472917821194E-4	0.0	0.0010100921984610787	9.871355575869632E-4
26-27	0.0	2.525230496152697E-4	0.0	0.0017906179881810032	0.0011822670050169442
28-29	0.0	2.754796904893851E-4	0.0	0.006600034251308184	0.001193745325454002
30-31	0.0	3.099146518005582E-4	0.0	0.02868432277220722	0.001193745325454002
32-33	0.0	3.213929722376159E-4	0.0	0.07606682953638146	0.0012970502093875213
34-35	0.0	3.213929722376159E-4	0.0	0.1409193400057575	0.0013314851706986946
36-37	0.0	3.213929722376159E-4	0.0	0.25241974473133616	0.0013314851706986946
38-39	0.0	3.9026289485996217E-4	0.0	0.43921793152401334	0.0013314851706986946
40-41	0.0	3.9026289485996217E-4	0.0	0.6539887852218003	0.0013544418115728099
42-43	0.0	3.9026289485996217E-4	0.0	0.872547484663816	0.0013888767728839832
44-45	0.0	3.9026289485996217E-4	0.0	1.1365144197548323	0.0014118334137580985
46-47	0.0	4.017412152970199E-4	0.0	1.4544179825795827	0.0014233117341951563
48-49	0.0	4.476544970452508E-4	0.0	1.7966555847308954	0.001469225015943387
50-51	0.0	4.5913281748230847E-4	0.0	2.1693796059630337	0.0015266166181286756
52-53	2.2956640874115422E-5	4.820894583564239E-4	0.0	2.545994777823334	0.001629921502062195
54-55	2.2956640874115422E-5	5.394810605417125E-4	0.0	2.964907560494192	0.001629921502062195
56-57	2.2956640874115422E-5	5.509593809787702E-4	0.0	3.4494992927058945	0.0016643564633733682
58-59	2.2956640874115422E-5	5.96872662727001E-4	0.0	3.977869339064535	0.0017217480655586568
60-61	2.2956640874115422E-5	5.96872662727001E-4	0.0	4.533798832792551	0.0017791396677439453
62-63	2.2956640874115422E-5	5.96872662727001E-4	0.0	5.0883624063885575	0.0018594879108033494
64-65	2.2956640874115422E-5	6.657425853493473E-4	0.0	5.6819981827523085	0.0018939228721145222
66-67	2.2956640874115422E-5	6.657425853493473E-4	0.0	6.356418378352071	0.001916879512988638
68-69	2.2956640874115422E-5	6.657425853493473E-4	0.0	7.100810415336138	0.0019283578334256955
70-71	2.2956640874115422E-5	6.657425853493473E-4	0.0	7.877605750914019	0.0019398361538627532
72-73	2.2956640874115422E-5	6.657425853493473E-4	0.0	8.663721482686789	0.002031662717359215
74-75	2.2956640874115422E-5	6.657425853493473E-4	0.0	9.464472073016811	0.0021234892808556765
76-77	2.2956640874115422E-5	6.657425853493473E-4	0.0	10.326815330812082	0.0021349676012927346
78-79	2.2956640874115422E-5	6.657425853493473E-4	0.0	11.284061341980946	0.00215792424216685
80-81	2.2956640874115422E-5	6.657425853493473E-4	0.0	12.27033602553513	0.0022153158443521384
82-83	2.2956640874115422E-5	6.657425853493473E-4	0.0	13.262958220291008	0.0022841857669744846
84-85	2.2956640874115422E-5	6.772209057864049E-4	0.0	14.268068827682402	0.002387490650908004
86	2.2956640874115422E-5	6.886992262234627E-4	0.0	15.029575040438123	0.002387490650908004
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8175	0.0	33.552917	1
GGTATCA	5210	0.0	29.680649	1
TCAACGC	9695	0.0	27.911758	4
CAACGCA	9645	0.0	27.723581	5
ATCAACG	9790	0.0	27.547209	3
TATCAAC	10085	0.0	27.061634	2
AACGCAG	10225	0.0	26.551954	6
GTACATG	13120	0.0	26.203457	1
TACATGG	13535	0.0	24.67096	2
ACATGGG	13725	0.0	23.759663	3
ACGCAGA	11795	0.0	22.859547	7
CGCAGAG	12265	0.0	21.720104	8
GAGTACA	9510	0.0	19.84153	1
CATGGGG	9430	0.0	19.600916	4
AGTACAT	9740	0.0	18.130705	2
GCAGAGT	13910	0.0	17.898684	9
AGAGTAC	10350	0.0	17.72109	10-11
GTATTAG	1250	0.0	17.304523	1
GAGTACT	4870	0.0	16.15207	12-13
GTGTAGC	2390	0.0	15.982768	1
>>END_MODULE