FastQCFastQC Report
Mon 15 Aug 2016
SRR2920897_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920897_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4356038
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82050.034.023861
GGTATCA52100.033.683151
TCAACGC101850.026.6157934
ATCAACG103050.026.259793
CAACGCA103050.026.0725125
GTACATG143100.025.9226281
TATCAAC106000.025.7523212
AACGCAG107800.025.012326
TACATGG146900.024.8841782
ACATGGG149250.023.7931333
ACGCAGA124850.021.329657
CATGGGG102200.020.2999464
CGCAGAG130950.020.0797738
GAGTACA106950.019.5657251
AGTACAT107500.018.2387872
AGAGTAC110950.018.11499410-11
GCAGAGT146050.016.931129
TAAGGTG19350.016.191145
TAAGGCG12100.015.6923895
GAGTACT48350.015.26128412-13
Produced by FastQC (version 0.11.5)