##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920897_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4356038
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.226960830001943	31.0	30.0	34.0	26.0	34.0
2	30.239136343622345	31.0	30.0	34.0	26.0	34.0
3	30.377695511379837	31.0	30.0	34.0	27.0	34.0
4	34.021047796185435	35.0	35.0	37.0	30.0	37.0
5	33.54450535096342	35.0	35.0	37.0	28.0	37.0
6	33.48591105036274	35.0	35.0	37.0	28.0	37.0
7	33.29567005613817	35.0	33.0	37.0	27.0	37.0
8	33.22340048456878	35.0	33.0	37.0	27.0	37.0
9	34.553771569485846	38.0	34.0	39.0	27.0	39.0
10-11	34.364654761964886	37.5	34.0	39.0	27.0	39.0
12-13	34.182931714553455	37.0	34.0	39.0	26.0	39.0
14-15	35.07713901944841	38.0	34.0	40.0	26.0	41.0
16-17	34.949372112915455	38.0	34.0	40.0	25.0	41.0
18-19	34.86114251069435	38.0	34.0	40.0	25.0	41.0
20-21	34.74424649188092	38.0	34.0	40.0	25.0	41.0
22-23	34.59190610825709	38.0	33.5	40.0	24.0	41.0
24-25	34.41560243505681	38.0	33.0	40.0	24.0	41.0
26-27	34.232545147677776	38.0	33.0	40.0	23.0	41.0
28-29	34.17316458212715	38.0	33.0	40.0	22.5	41.0
30-31	34.16576129501166	38.0	33.0	40.0	22.5	41.0
32-33	34.01818923526379	38.0	33.0	40.0	22.0	41.0
34-35	33.84402959753794	38.0	33.0	40.0	20.5	41.0
36-37	33.64940262229118	38.0	32.5	40.0	19.0	41.0
38-39	33.457425187750886	38.0	32.0	40.0	18.0	41.0
40-41	33.230560086941395	38.0	32.0	40.0	16.5	41.0
42-43	33.258254863708714	38.0	32.0	40.0	16.0	41.0
44-45	33.1140169116982	38.0	32.0	40.0	15.0	41.0
46-47	32.86937017078363	37.5	31.5	40.0	13.5	41.0
48-49	32.6071859336397	37.0	31.0	40.0	10.0	41.0
50-51	32.34645542577911	37.0	31.0	40.0	8.5	41.0
52-53	32.0855402317427	37.0	31.0	40.0	8.0	41.0
54-55	31.80024657727963	36.0	30.0	40.0	7.0	41.0
56-57	31.47276378213413	36.0	30.0	39.0	4.0	41.0
58-59	31.138874936352714	35.5	29.0	39.0	2.0	40.0
60-61	30.803503091570825	35.0	29.0	39.0	2.0	40.0
62-63	30.484752887830638	35.0	28.5	39.0	2.0	40.0
64-65	30.08223803373616	35.0	28.0	38.0	2.0	40.0
66-67	29.64516402290338	34.0	28.0	38.0	2.0	40.0
68-69	29.19996003248824	34.0	27.5	37.0	2.0	39.5
70-71	28.76909958085765	34.0	26.5	36.5	2.0	39.0
72-73	28.33678723647498	34.0	26.0	36.0	2.0	39.0
74-75	27.900901690940252	33.0	26.0	35.5	2.0	38.5
76-77	26.457636503630134	31.5	24.5	34.0	2.0	36.0
78-79	27.299537331859824	33.0	26.0	35.0	2.0	37.0
80-81	27.326187007551354	33.0	26.0	35.0	2.0	36.5
82-83	26.995461357315982	33.0	25.0	35.0	2.0	36.0
84-85	26.679966634818154	33.0	25.0	35.0	2.0	36.0
86-87	26.14776501031442	32.0	24.0	35.0	2.0	35.0
88-89	25.779895285578316	32.0	22.5	35.0	2.0	35.0
90-91	25.419352631910005	32.0	20.0	34.5	2.0	35.0
92-93	25.11628445390054	32.0	19.5	34.0	2.0	35.0
94-95	24.731788037661744	32.0	17.5	34.0	2.0	35.0
96-97	24.207816254128176	32.0	6.0	34.0	2.0	35.0
98-99	23.608015701424094	31.0	2.0	34.0	2.0	35.0
100-101	21.418073832230114	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103109.0
3	47093.0
4	25338.0
5	17957.0
6	15363.0
7	15495.0
8	17399.0
9	20061.0
10	22588.0
11	25245.0
12	28010.0
13	30352.0
14	33041.0
15	34704.0
16	36698.0
17	38529.0
18	37286.0
19	35758.0
20	36368.0
21	37910.0
22	41285.0
23	46690.0
24	54773.0
25	64209.0
26	75703.0
27	90213.0
28	106283.0
29	125062.0
30	146683.0
31	174409.0
32	208062.0
33	253483.0
34	319849.0
35	413032.0
36	548269.0
37	636551.0
38	367428.0
39	25749.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47847615402591	19.237966610668174	13.333261265173416	24.9502959701325
2	16.284867120075628	20.514972550744506	37.810000739203836	25.39015958997603
3	19.305241010438305	24.066926017151662	29.324765767205392	27.303067205204645
4	13.682584734662258	16.289227585950382	35.94318289572441	34.085004783662946
5	14.450062320079326	37.01489001062736	34.14942906358714	14.385618605706174
6	33.86436082629879	35.243609044327464	16.81392236839656	14.07810776097719
7	29.479760680349738	31.467761594373144	20.655158921912147	18.397318803364968
8	26.856617896336786	34.46868748995875	19.84429993465571	18.830394679048755
9	26.71900255150657	15.449361939448444	19.28636896867778	38.54526654036721
10-11	25.807845012346707	25.235962965733616	27.585049456357552	21.37114256556212
12-13	26.38965241452854	22.509767510273125	27.411862583770763	23.688717491427575
14-15	23.378856298137652	24.21739831912378	24.815504897192962	27.588240485545608
16-17	23.63282499519036	27.176739151034145	25.66113151418915	23.529304339586343
18-19	23.668548107699785	26.397123894821874	26.910059337991292	23.02426865948705
20-21	24.467667460223687	25.575064741323487	26.58567585371682	23.371591944736007
22-23	24.012734851086126	25.50890983512678	26.519245241576293	23.959110072210795
24-25	23.808355095102126	25.69209085941396	26.612528117644707	23.887025927839208
26-27	23.69639357887027	25.85077510988127	26.57418190183982	23.87864940940864
28-29	23.705269361019415	26.068267030931423	26.45772886006657	23.768734747982588
30-31	23.92273951993372	25.816892010116234	26.20189934194906	24.05846912800099
32-33	23.73517374030614	25.7901080880862	26.25326401035757	24.22145416125009
34-35	24.090125595008622	25.846076944077513	26.262832680740146	23.800964780173718
36-37	24.18570362630387	25.58788272207981	26.20712902483977	24.019284626776546
38-39	23.978878665077254	25.686559353004114	26.3240160113938	24.010545970524834
40-41	24.09262177615887	25.626203088586585	26.451094470482584	23.83008066477196
42-43	23.981290150753306	25.53345685645144	26.290621426898653	24.1946315658966
44-45	23.845633654044867	25.585455463960137	26.399572894899997	24.169337987095
46-47	23.98433162844396	25.583559577071085	26.225186625966714	24.20692216851824
48-49	23.7933439601364	25.60123591112515	26.21298115345736	24.392438975281088
50-51	23.693467152078	25.75305188292819	26.332041739986558	24.221439225007256
52-53	23.924961321037795	25.52215738038679	26.342709265296012	24.210172033279402
54-55	23.55306551464774	25.464207881374378	26.471948926916046	24.51077767706184
56-57	23.534500966522952	25.630317598064202	26.30303823390315	24.532143201509705
58-59	23.471090355523863	25.442516578731933	26.34506628049153	24.741326785252678
60-61	23.356520503188644	25.538634140259397	26.523406703433807	24.581438653118155
62-63	23.22105327926344	25.612734603316145	26.47919447093016	24.68701764649025
64-65	23.113115930616388	25.53620223126983	26.277008700730946	25.07367313738284
66-67	23.092358802002945	25.562362771656282	26.17823892765849	25.167039498682275
68-69	23.375601049497273	25.320454420647682	26.195004846997332	25.108939682857713
70-71	23.149004311927385	25.14649967888437	26.26343559513392	25.441060414054324
72-73	23.0526455161035	25.087253336285322	26.330061460017916	25.530039687593266
74-75	22.932572550590088	25.270235604388837	26.1390631908968	25.658128654124273
76-77	22.98791773172543	24.95480583856442	26.4247796673162	25.63249676239395
78-79	22.891418588467154	25.0488620602271	26.34709833916094	25.712621012144808
80-81	22.861094021525187	24.990388184188223	26.228346435901962	25.920171358384632
82-83	22.726803627246294	24.94164035627766	26.333765388894204	25.997790627581846
84-85	22.58327143819804	24.868665602108607	26.309653230352076	26.238409729341278
86-87	22.549472832594788	24.826658794975238	26.31309652661616	26.310771845813818
88-89	22.51484449359981	24.70766358279105	26.219121830584687	26.558370093024454
90-91	22.263787488436723	24.72321215456617	26.24654943074958	26.766450926247526
92-93	22.33556389562286	24.542643348778405	26.302946097675544	26.81884665792319
94-95	22.068119910086832	24.408017896315194	26.462377414223965	27.061484779374013
96-97	22.121303255843852	24.20270623941082	26.50485782371334	27.171132681031988
98-99	21.984789271863526	24.195402391208376	26.382771031203163	27.43703730572494
100-101	22.069811238019717	23.952518054650312	26.544634823934878	27.433035883395096
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	58.0
2	88.0
3	126.0
4	113.0
5	117.0
6	196.5
7	413.0
8	655.0
9	704.5
10	595.5
11	530.5
12	532.0
13	555.5
14	627.0
15	698.0
16	774.5
17	884.0
18	1007.0
19	1199.5
20	1387.0
21	1615.5
22	1972.0
23	2400.5
24	3010.0
25	3770.0
26	4862.0
27	6559.5
28	8634.0
29	11605.0
30	16041.0
31	22580.5
32	31567.5
33	40122.5
34	48278.5
35	58332.5
36	71204.0
37	84006.0
38	95896.5
39	107227.5
40	117080.0
41	127027.5
42	137613.0
43	148652.0
44	159101.5
45	169544.5
46	179232.5
47	186411.0
48	192120.5
49	196184.5
50	195531.5
51	192830.5
52	190067.5
53	188312.5
54	184382.0
55	173160.0
56	158161.5
57	142180.5
58	128591.5
59	113423.0
60	94748.0
61	78594.0
62	64933.0
63	49912.0
64	36583.5
65	28015.5
66	21504.0
67	16750.0
68	13151.5
69	10098.5
70	7777.5
71	6091.5
72	4718.5
73	3784.0
74	2938.5
75	2131.0
76	1459.5
77	949.0
78	593.0
79	329.0
80	189.5
81	96.0
82	49.5
83	25.5
84	9.0
85	8.5
86	5.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19058603253690626
2	0.0
3	0.01244249935377056
4	0.013521461474853985
5	0.006864035621360512
6	0.10415427964586169
7	0.13865811087965715
8	0.12022392825774247
9	0.13909428705626534
10-11	0.1119939725043721
12-13	0.11198249418393504
14-15	0.11418633170785011
16-17	0.06429007276796024
18-19	0.0768129203647902
20-21	0.03658140723290293
22-23	0.08201259952277734
24-25	0.05635855334595336
26-27	0.0646573790219461
28-29	0.03893446292249976
30-31	0.024242212763065887
32-33	0.03096850853918171
34-35	0.019490188102123995
36-37	0.024483257492244098
38-39	0.03188677417414632
40-41	0.02527526160240108
42-43	0.013268938425238713
44-45	0.026124657314743352
46-47	0.03226555874856923
48-49	0.008838306736534438
50-51	0.015931908766636103
52-53	0.0071395153118498965
54-55	0.012717979044259944
56-57	0.005004547710557162
58-59	0.003581235976362006
60-61	0.022302376609203135
62-63	0.010123878625484901
64-65	0.007162471952724012
66-67	0.04025446977276139
68-69	0.08858967713321142
70-71	0.02086758655457092
72-73	0.04376683582650105
74-75	0.08444600345543359
76-77	0.24381100440354284
78-79	0.07986615360104755
80-81	0.01656321639067428
82-83	0.011535712039243001
84-85	0.01269502240338583
86-87	0.2606841354460177
88-89	0.6177058143202607
90-91	0.2631978876217333
92-93	0.3507545159156095
94-95	0.0013200068502616368
96-97	0.004086282075592545
98-99	0.0037878457442290445
100-101	0.005394810605417124
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4356038.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.58421920472722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.23814699778507	37.263484096770526
2	13.453177775118693	13.879433427037743
3	5.512314068414188	8.530452516911376
4	2.8415941657979644	5.863256653575847
5	1.6358684269843655	4.219249776382691
6	1.0316710484002296	3.193076730470892
7	0.6993006001137859	2.525101281238678
8	0.5020368718141921	2.0717744035615064
9	0.3510325534612947	1.6296966167168299
>10	1.6294683956926153	14.31542886799969
>50	0.0739865243102342	2.59129765323913
>100	0.028898617574131008	2.593950032897026
>500	0.0016096853294125127	0.5648642442928998
>1k	8.942692037666396E-4	0.7589336989051381
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	2.2956640874115422E-5
4	0.0	2.2956640874115422E-5	0.0	0.0	2.2956640874115422E-5
5	0.0	2.2956640874115422E-5	0.0	0.0	2.2956640874115422E-5
6	0.0	2.2956640874115422E-5	0.0	0.0	1.1478320437057712E-4
7	0.0	2.2956640874115422E-5	0.0	2.2956640874115422E-5	1.1478320437057712E-4
8	0.0	2.2956640874115422E-5	0.0	2.2956640874115422E-5	1.1478320437057712E-4
9	0.0	4.5913281748230844E-5	0.0	4.5913281748230844E-5	1.1478320437057712E-4
10-11	0.0	4.5913281748230844E-5	0.0	4.5913281748230844E-5	1.6069648611880796E-4
12-13	0.0	6.886992262234627E-5	0.0	5.739160218528856E-5	4.3617617660819306E-4
14-15	0.0	6.886992262234627E-5	1.1478320437057711E-5	9.182656349646169E-5	5.394810605417125E-4
16-17	0.0	6.886992262234627E-5	2.2956640874115422E-5	1.1478320437057712E-4	6.198293036011164E-4
18-19	0.0	6.886992262234627E-5	2.2956640874115422E-5	1.3773984524469255E-4	6.427859444752318E-4
20-21	0.0	6.886992262234627E-5	2.2956640874115422E-5	2.4104472917821194E-4	6.772209057864049E-4
22-23	0.0	6.886992262234627E-5	2.2956640874115422E-5	4.3617617660819306E-4	7.805257897199243E-4
24-25	0.0	9.182656349646169E-5	2.2956640874115422E-5	0.001033048839335194	8.034824305940398E-4
26-27	0.0	1.3773984524469255E-4	2.2956640874115422E-5	0.0018135746290551184	8.608740327793284E-4
28-29	0.0	1.3773984524469255E-4	2.2956640874115422E-5	0.00677220905786405	8.723523532163861E-4
30-31	0.0	1.3773984524469255E-4	2.2956640874115422E-5	0.029292673755371278	8.953089940905016E-4
32-33	0.0	1.6069648611880796E-4	2.2956640874115422E-5	0.07695066021003491	9.871355575869632E-4
34-35	0.0	1.6069648611880796E-4	2.2956640874115422E-5	0.14262960975087913	0.0011019187619575404
36-37	0.0	1.6069648611880796E-4	2.2956640874115422E-5	0.2545317556917548	0.0011019187619575404
38-39	0.0	1.8365312699292338E-4	2.2956640874115422E-5	0.4437748247375253	0.001147832043705771
40-41	0.0	1.8365312699292338E-4	2.2956640874115422E-5	0.6622531759364818	0.001147832043705771
42-43	0.0	1.8365312699292338E-4	2.2956640874115422E-5	0.8846111994431637	0.001147832043705771
44-45	0.0	1.8365312699292338E-4	2.2956640874115422E-5	1.1512181482347033	0.0011937453254540018
46-47	0.0	1.8365312699292338E-4	2.2956640874115422E-5	1.472381554063578	0.001274093568513406
48-49	0.0	2.4104472917821194E-4	2.2956640874115422E-5	1.8158932497834042	0.0014118334137580985
50-51	0.0	2.525230496152697E-4	2.2956640874115422E-5	2.1857476909062776	0.0014921816568175025
52-53	0.0	2.525230496152697E-4	2.2956640874115422E-5	2.559745805706929	0.0015840082203139642
54-55	0.0	2.525230496152697E-4	2.2956640874115422E-5	2.9770860584779104	0.0016184431816251375
56-57	0.0	2.6400137005232736E-4	2.2956640874115422E-5	3.4603807404802254	0.0016873131042474837
58-59	0.0	3.213929722376159E-4	2.2956640874115422E-5	3.9869027772485	0.0017561830268698298
60-61	0.0	3.213929722376159E-4	2.2956640874115422E-5	4.541018696347461	0.0017676613473068877
62-63	0.0	3.213929722376159E-4	2.2956640874115422E-5	5.094296698054516	0.0018365312699292339
64-65	0.0	3.213929722376159E-4	2.2956640874115422E-5	5.687128991987674	0.00190540119255158
66-67	1.1478320437057711E-5	3.213929722376159E-4	2.2956640874115422E-5	6.359448654947455	0.0019742711151739265
68-69	2.2956640874115422E-5	3.213929722376159E-4	2.2956640874115422E-5	7.100936676860945	0.0019972277560480417
70-71	2.2956640874115422E-5	3.213929722376159E-4	2.2956640874115422E-5	7.873416163954493	0.0020431410377962727
72-73	2.2956640874115422E-5	3.213929722376159E-4	2.2956640874115422E-5	8.658085627352195	0.002134967601292734
74-75	2.2956640874115422E-5	3.4434961311173134E-4	2.2956640874115422E-5	9.462888064796497	0.0022497508056633113
76-77	2.2956640874115422E-5	3.4434961311173134E-4	2.2956640874115422E-5	10.326918635696014	0.002318620728285658
78-79	2.2956640874115422E-5	3.4434961311173134E-4	2.2956640874115422E-5	11.285702741803446	0.002341577369159773
80-81	2.2956640874115422E-5	3.4434961311173134E-4	2.2956640874115422E-5	12.275673444538363	0.0023989689713450617
82-83	2.2956640874115422E-5	3.4434961311173134E-4	2.2956640874115422E-5	13.271922788552349	0.0026170570596491583
84-85	2.2956640874115422E-5	3.4434961311173134E-4	2.2956640874115422E-5	14.28087863329016	0.0026400137005232736
86-87	2.2956640874115422E-5	3.6730625398584676E-4	2.2956640874115422E-5	15.316567486325877	0.0027088836231456198
88-89	2.2956640874115422E-5	3.6730625398584676E-4	2.2956640874115422E-5	16.416615282052177	0.002789231866205024
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8205	0.0	34.02386	1
GGTATCA	5210	0.0	33.68315	1
TCAACGC	10185	0.0	26.615793	4
ATCAACG	10305	0.0	26.25979	3
CAACGCA	10305	0.0	26.072512	5
GTACATG	14310	0.0	25.922628	1
TATCAAC	10600	0.0	25.752321	2
AACGCAG	10780	0.0	25.01232	6
TACATGG	14690	0.0	24.884178	2
ACATGGG	14925	0.0	23.793133	3
ACGCAGA	12485	0.0	21.32965	7
CATGGGG	10220	0.0	20.299946	4
CGCAGAG	13095	0.0	20.079773	8
GAGTACA	10695	0.0	19.565725	1
AGTACAT	10750	0.0	18.238787	2
AGAGTAC	11095	0.0	18.114994	10-11
GCAGAGT	14605	0.0	16.93112	9
TAAGGTG	1935	0.0	16.19114	5
TAAGGCG	1210	0.0	15.692389	5
GAGTACT	4835	0.0	15.261284	12-13
>>END_MODULE