FastQCFastQC Report
Mon 15 Aug 2016
SRR2920896_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920896_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369543
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26950.043.0307351
GTATCAA48900.042.8192981
TCAACGC62400.032.5560534
ATCAACG63450.032.089583
CAACGCA63350.031.7782925
AACGCAG65850.030.7743516
TATCAAC70300.029.0945432
GTACATG114000.027.934331
TACATGG116100.026.4256632
ACATGGG120300.025.158863
ACGCAGA80350.024.5905747
CGCAGAG82650.023.6843978
CATGGGG82650.020.9175664
GAGTACA84350.020.7862851
AGTACAT81800.020.5752332
GCAGAGT98850.019.1060989
TAAGGCG8950.018.4455725
AGAGTAC80350.017.54366910-11
GTATTAA15750.017.2388551
TAAGGTG11950.016.5011125
Produced by FastQC (version 0.11.5)