##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920896_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3369543
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.21705940538524	31.0	28.0	31.0	16.0	34.0
2	28.223459679843824	31.0	28.0	33.0	16.0	34.0
3	28.302891816486685	31.0	28.0	34.0	16.0	34.0
4	31.751487071095397	35.0	32.0	37.0	19.0	37.0
5	31.69411460248467	35.0	32.0	37.0	19.0	37.0
6	31.811148277377676	35.0	32.0	37.0	19.0	37.0
7	31.632338569354953	35.0	32.0	37.0	18.0	37.0
8	31.56566662007281	35.0	32.0	37.0	17.0	37.0
9	32.79263538111845	37.0	32.0	39.0	17.0	39.0
10-11	32.57924234829471	37.0	32.0	39.0	17.0	39.0
12-13	32.39235142569779	37.0	32.0	39.0	16.0	39.0
14-15	33.107451514938376	38.0	32.0	40.0	14.5	41.0
16-17	32.97730879231991	38.0	32.0	40.0	11.5	41.0
18-19	32.83543910850818	38.0	32.0	40.0	10.0	41.0
20-21	32.72800881306456	38.0	31.5	40.0	10.0	41.0
22-23	32.48917152860195	37.5	31.0	40.0	10.0	41.0
24-25	32.278957561900825	37.0	31.0	40.0	9.5	41.0
26-27	32.306259780629006	37.0	31.0	40.0	8.0	41.0
28-29	32.133787875685215	37.0	30.5	40.0	8.0	41.0
30-31	31.874536398556124	37.0	30.0	40.0	4.5	41.0
32-33	31.61639768360279	37.0	30.0	40.0	2.0	41.0
34-35	31.280859155084237	37.0	30.0	40.0	2.0	41.0
36-37	30.96613012506444	36.0	29.5	40.0	2.0	41.0
38-39	30.745891208392354	36.0	29.0	40.0	2.0	41.0
40-41	30.527310083296165	36.0	28.5	39.0	2.0	41.0
42-43	30.278614933835243	36.0	28.0	39.0	2.0	40.0
44-45	29.992216748680754	35.0	27.0	39.0	2.0	40.0
46-47	29.727568397257432	35.0	27.0	39.0	2.0	40.0
48-49	29.41113142049233	35.0	26.0	39.0	2.0	40.0
50-51	28.285535902049624	34.0	24.5	38.0	2.0	39.5
52-53	28.735297783705388	34.5	25.5	38.0	2.0	39.5
54-55	29.359342646762485	35.0	27.0	39.0	2.0	40.0
56-57	29.28902643474204	35.0	26.0	39.0	2.0	40.0
58-59	29.035183851341266	35.0	26.0	39.0	2.0	40.0
60-61	28.69613149320249	34.0	26.0	38.5	2.0	40.0
62-63	28.29095384745053	34.0	25.5	38.0	2.0	40.0
64-65	27.855206625943044	34.0	24.0	37.5	2.0	40.0
66-67	27.456408628707216	34.0	23.0	37.0	2.0	40.0
68-69	27.003661327366945	33.0	22.0	36.5	2.0	39.0
70-71	26.659098726444505	33.0	21.5	36.0	2.0	39.0
72-73	26.19735569482271	32.5	20.0	35.5	2.0	38.5
74-75	25.755616414451456	32.0	18.5	35.0	2.0	37.5
76-77	25.34066444618751	32.0	17.5	35.0	2.0	37.0
78-79	24.88106918356584	32.0	13.0	35.0	2.0	36.5
80-81	24.428466115434645	31.0	7.5	35.0	2.0	36.0
82-83	23.954253737079476	31.0	4.5	34.0	2.0	35.5
84-85	23.54555350681086	31.0	2.0	34.0	2.0	35.0
86-87	23.08304657337805	31.0	2.0	34.0	2.0	35.0
88-89	22.540487834700432	30.0	2.0	34.0	2.0	35.0
90-91	22.022849834532458	30.0	2.0	34.0	2.0	35.0
92-93	21.46949393434065	29.0	2.0	34.0	2.0	35.0
94-95	20.809291052228744	29.0	2.0	34.0	2.0	35.0
96-97	20.149380049460714	29.0	2.0	34.0	2.0	35.0
98	19.73510680825263	27.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	199540.0
3	34278.0
4	25085.0
5	20444.0
6	18872.0
7	19515.0
8	22197.0
9	24968.0
10	27264.0
11	28744.0
12	29535.0
13	28738.0
14	27056.0
15	27984.0
16	29640.0
17	31787.0
18	32966.0
19	33288.0
20	34861.0
21	37153.0
22	40321.0
23	44970.0
24	51389.0
25	57924.0
26	66537.0
27	76877.0
28	87810.0
29	101729.0
30	116996.0
31	136133.0
32	161304.0
33	194173.0
34	238100.0
35	296621.0
36	365055.0
37	379396.0
38	202388.0
39	17905.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3781387422388	17.23153102692138	12.069305573762676	25.32102465707714
2	16.634389035500448	19.16985534436235	37.97298059483063	26.22277502530657
3	19.69437556286818	23.038992480051387	28.240087434912102	29.026544522168322
4	13.588412900253727	14.771320052193715	35.24369929486244	36.396567752690125
5	15.03054363484542	36.30428400489553	33.54109357463087	15.124078785628182
6	35.10408404614559	33.734522752207305	16.62883933108843	14.532553870558676
7	30.509355987603513	30.641857466520673	20.303566112271852	18.545220433603966
8	27.626420801941336	33.52742352541713	19.58581560620487	19.26034006643666
9	27.469481766518488	15.017852569324683	18.834987415207344	38.67767824894949
10-11	26.388819847204775	24.569210805324463	26.973157335157516	22.068812012313245
12-13	26.890390425372622	21.8513526367814	26.958400303202666	24.29985663464331
14-15	24.003267006396158	23.48478406907012	24.182094650291866	28.32985427424186
16-17	24.474144592262558	26.362049461564247	25.010272772722637	24.153533173450555
18-19	24.508687114830472	25.624969940522313	26.223606294172587	23.642736650474628
20-21	25.153745308785563	25.00607377873981	25.88224235000885	23.95793856246577
22-23	24.81060628565521	24.660944999031567	25.851090881652915	24.677357833660302
24-25	24.565257287979964	25.000683020757297	25.82914265452751	24.604917036735234
26-27	24.32634950617229	25.231960178968926	25.768094765025328	24.67359554983346
28-29	24.38181394361325	25.33344617216533	25.80447405968877	24.480265824532648
30-31	24.544104852766075	25.188759921253528	25.595483064461057	24.671652161519344
32-33	24.318411291839073	25.196731075398898	25.483993100962028	25.000864531800005
34-35	24.811483640573854	25.14131654429457	25.59346695000453	24.453732865127048
36-37	24.864152728908692	24.913154407346703	25.532663907258097	24.690028956486508
38-39	24.699338506693586	25.05102660808926	25.52666362994545	24.722971255271705
40-41	24.77451671288715	25.019466880357495	25.687752730209468	24.51826367654589
42-43	24.684547593959078	24.93387802795112	25.59958387795343	24.781990500136374
44-45	24.476524452252118	25.01272099705797	25.703685326860708	24.807069223829203
46-47	24.631881839821965	24.973135390480007	25.62952287976694	24.76545988993109
48-49	24.56083188484693	25.078175060587625	25.44178276532852	24.919210289236922
50-51	24.362761456409036	25.22108336809487	25.63986055658185	24.77629461891425
52-53	24.55908455532203	24.947933584555994	25.776449597429078	24.716532262692898
54-55	24.230699453305903	24.927300999576783	25.96699323016413	24.875006316953183
56-57	24.122063778455384	25.02233540982478	25.846901365190917	25.008699446528915
58-59	24.160441700883368	24.682662340332833	25.938766363072308	25.218129595711492
60-61	24.048603871886908	24.744860968396456	26.123113217037137	25.0834219426795
62-63	23.921997564193223	24.86279589733137	26.13496407825692	25.080242460218482
64-65	23.932263149248673	24.692460959289857	25.984048904528663	25.391226986932814
66-67	23.949114236067935	24.75473641489184	26.003009453297558	25.293139895742662
68-69	24.18700562058782	24.532737785674964	25.973425071594768	25.30683152214245
70-71	23.87882028668395	24.56404959139308	26.05210417856715	25.505025943355825
72-73	24.017742932114253	24.53237076854317	26.005214637263702	25.44467166207888
74-75	23.986610035832044	24.605302499666458	26.042024034154803	25.366063430346692
76-77	24.02284663986762	24.406383586679453	26.132806107764083	25.43796366568884
78-79	23.999092310638222	24.42370808376591	26.086896962069723	25.490302643526146
80-81	24.053530325630604	24.458066660630294	26.064402351326493	25.42400066241261
82-83	24.027916993599437	24.394056879675684	26.146575385100622	25.431450741624257
84-85	23.97436955130334	24.326618455197792	26.172805383895316	25.52620660960356
86-87	24.01010162974469	24.263878201539455	26.17518325991819	25.55083690879767
88-89	23.988145601449105	24.185147889505924	26.117215244178567	25.709491264866415
90-91	24.022592475380232	24.167291546437813	26.164752313048012	25.645363665133942
92-93	24.194456518841793	23.98684141359946	26.201079890417585	25.617622177141165
94-95	24.063584585473805	23.829878172732467	26.353692979797717	25.75284426199601
96-97	24.288634049896736	23.64142462025833	26.280345527837795	25.789595802007142
98	24.27285555753449	23.51927843397012	26.304523547572572	25.90334246092282
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	35.0
2	80.0
3	122.0
4	134.5
5	161.0
6	246.0
7	466.0
8	715.0
9	822.5
10	830.0
11	910.0
12	1048.0
13	1219.0
14	1390.5
15	1539.5
16	1688.0
17	1787.0
18	1797.5
19	1778.0
20	1798.5
21	1786.0
22	1872.0
23	2090.0
24	2374.0
25	2929.5
26	3812.5
27	4982.0
28	6536.0
29	8877.5
30	12313.0
31	17552.5
32	23312.5
33	29210.0
34	35751.5
35	43226.0
36	51936.0
37	60777.5
38	69322.0
39	77547.0
40	85213.5
41	92405.5
42	101992.5
43	113780.0
44	122663.5
45	130115.0
46	138978.5
47	146285.5
48	151383.0
49	153038.0
50	152476.0
51	152786.0
52	152575.0
53	153646.0
54	151956.5
55	145815.0
56	136649.5
57	124762.5
58	114875.5
59	102602.0
60	88038.5
61	73882.0
62	60174.5
63	46751.5
64	35069.0
65	26885.0
66	20442.0
67	15864.0
68	12381.5
69	9684.0
70	7525.0
71	6050.0
72	5143.0
73	4310.5
74	3527.0
75	2744.0
76	1940.0
77	1167.0
78	658.5
79	383.5
80	215.5
81	126.0
82	88.5
83	60.5
84	35.0
85	18.5
86	9.5
87	12.5
88	12.0
89	5.5
90	4.5
91	6.5
92	4.5
93	1.0
94	0.5
95	1.0
96	3.0
97	3.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3888657898118528
2	0.025315005625391927
3	0.018904640777695967
4	0.016471076344774353
5	0.02243627696693587
6	0.021575626130902616
7	0.015551070278669836
8	0.009467159196365798
9	0.0
10-11	1.483880751781473E-5
12-13	0.005327131898895488
14-15	0.0028935674659738724
16-17	0.005920684199608077
18-19	0.03623636795850357
20-21	0.07748825285802852
22-23	0.09719418924168649
24-25	0.0637771947115677
26-27	0.0735411300582898
28-29	0.056624889487981014
30-31	0.05261841145817103
32-33	0.019809808036282666
34-35	0.3406693429939906
36-37	0.5228305440826841
38-39	0.4791599335577555
40-41	0.4870690179647507
42-43	0.43798224269581953
44-45	0.4116433593516985
46-47	0.4103523830976486
48-49	0.3992677938818409
50-51	0.39598841742040386
52-53	0.4574359193516747
54-55	0.4592165762538124
56-57	0.4286783103821497
58-59	0.41456660443270793
60-61	0.4291531522227198
62-63	0.40883882473083144
64-65	0.45751011338926373
66-67	0.4781063782239906
68-69	0.4214221335059384
70-71	0.3772173259103683
72-73	0.36392175437440627
74-75	0.3458926032402614
76-77	0.3438893642253564
78-79	0.3432512955020903
80-81	0.35966301661679345
82-83	0.3545436280231473
84-85	0.35535976243662715
86-87	0.34043192207370554
88-89	0.38530447600757733
90-91	0.3629720706932661
92-93	0.3527926487360452
94-95	0.359855921114525
96-97	0.35995979276714973
98	0.35485524298102145
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3369543.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.92484481340143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.19536119860537	38.525826161118495
2	12.01593756277323	12.478513864690417
3	5.013186115732136	7.809267332402695
4	2.7499254245754945	5.7115780367803834
5	1.5894981006933608	4.126722110484954
6	1.0364193693363575	3.228954894863625
7	0.715552664799132	2.600847275285754
8	0.505009045672466	2.09780130607254
9	0.36470150686868275	1.7043362232633004
>10	1.706116782134012	15.1060628890264
>50	0.07212909604363359	2.5213417549463415
>100	0.03414750677093045	3.2265502818546223
>500	0.0015549114832297097	0.5515937262956766
>1k	4.6071451202143153E-4	0.31060414291490746
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7806569021377677E-4
2	0.0	0.0	0.0	0.0	2.374209202850357E-4
3	0.0	0.0	0.0	0.0	2.374209202850357E-4
4	0.0	0.0	0.0	0.0	2.374209202850357E-4
5	0.0	0.0	0.0	0.0	2.967761503562946E-4
6	0.0	0.0	0.0	0.0	3.5613138042755354E-4
7	0.0	0.0	0.0	0.0	3.5613138042755354E-4
8	0.0	0.0	0.0	0.0	3.5613138042755354E-4
9	0.0	0.0	0.0	0.0	3.5613138042755354E-4
10-11	0.0	0.0	0.0	0.0	4.0064780298099774E-4
12-13	0.0	0.0	0.0	0.0	0.00109807175631829
14-15	0.0	0.0	0.0	5.935523007125892E-5	0.0013058150615676964
16-17	0.0	0.0	0.0	8.903284510688838E-5	0.001543235981852732
18-19	0.0	0.0	0.0	1.6322688269596204E-4	0.001647107634477435
20-21	0.0	1.483880751781473E-5	0.0	2.2258211276722094E-4	0.0017954957096555824
22-23	0.0	2.967761503562946E-5	0.0	2.967761503562946E-4	0.001929044977315915
24-25	0.0	2.967761503562946E-5	0.0	6.825851458194777E-4	0.0019587225923515445
26-27	0.0	2.967761503562946E-5	0.0	0.0013948479066745846	0.002092271860011877
28-29	0.0	2.967761503562946E-5	0.0	0.005683263279323042	0.00219614351263658
30-31	0.0	2.967761503562946E-5	0.0	0.02617565646142518	0.00219614351263658
32-33	0.0	2.967761503562946E-5	0.0	0.06864432357741095	0.00219614351263658
34-35	0.0	2.967761503562946E-5	0.0	0.12667889977958435	0.00219614351263658
36-37	0.0	2.967761503562946E-5	0.0	0.22501567720014257	0.00219614351263658
38-39	0.0	2.967761503562946E-5	0.0	0.3889251450419241	0.0022258211276722094
40-41	0.0	2.967761503562946E-5	0.0	0.5781347797015797	0.0023296927802969125
42-43	0.0	2.967761503562946E-5	0.0	0.7729683224104871	0.002507758470510689
44-45	0.0	2.967761503562946E-5	0.0	1.0131492608938362	0.002700662968242281
46-47	0.0	2.967761503562946E-5	0.0	1.2979356547757366	0.0029529226960451314
48-49	0.0	2.967761503562946E-5	0.0	1.6054996181974825	0.0030567943486698346
50-51	0.0	2.967761503562946E-5	0.0	1.9490625286574468	0.003234860038883611
52-53	0.0	2.967761503562946E-5	0.0	2.2980416038614138	0.003368409306543944
54-55	0.0	2.967761503562946E-5	0.0	2.6809867094736584	0.0034426033441330175
56-57	0.0	2.967761503562946E-5	0.0	3.132056780400191	0.0034722809591686467
58-59	0.0	2.967761503562946E-5	0.0	3.6105341288121267	0.0034722809591686467
60-61	0.0	2.967761503562946E-5	0.0	4.12426848388639	0.0034722809591686467
62-63	0.0	2.967761503562946E-5	0.0	4.644576430691046	0.0035613138042755355
64-65	0.0	2.967761503562946E-5	0.0	5.1954523209824	0.003620669034346794
66-67	0.0	2.967761503562946E-5	0.0	5.825389377728671	0.003680024264418053
68-69	0.0	2.967761503562946E-5	0.0	6.516595870715999	0.0037097018794536828
70-71	0.0	2.967761503562946E-5	0.0	7.246368424442128	0.003724540686971497
72-73	0.0	2.967761503562946E-5	0.0	7.990757203573304	0.0038135735320783855
74-75	0.0	2.967761503562946E-5	0.0	8.7420757058153	0.003976800414774348
76-77	0.0	2.967761503562946E-5	0.0	9.549410706437044	0.00408067206739905
78-79	0.0	4.451642255344419E-5	0.0	10.42967844600885	0.004214221335059384
80-81	0.0	5.935523007125892E-5	0.0	11.35330518114771	0.0042884153726484575
82-83	0.0	8.903284510688838E-5	0.0	12.293521703091487	0.004451642255344419
84-85	0.0	8.903284510688838E-5	0.0	13.220427814691785	0.0044813198703800485
86	0.0	8.903284510688838E-5	0.0	13.933224772617534	0.0044813198703800485
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2695	0.0	43.030735	1
GTATCAA	4890	0.0	42.819298	1
TCAACGC	6240	0.0	32.556053	4
ATCAACG	6345	0.0	32.08958	3
CAACGCA	6335	0.0	31.778292	5
AACGCAG	6585	0.0	30.774351	6
TATCAAC	7030	0.0	29.094543	2
GTACATG	11400	0.0	27.93433	1
TACATGG	11610	0.0	26.425663	2
ACATGGG	12030	0.0	25.15886	3
ACGCAGA	8035	0.0	24.590574	7
CGCAGAG	8265	0.0	23.684397	8
CATGGGG	8265	0.0	20.917566	4
GAGTACA	8435	0.0	20.786285	1
AGTACAT	8180	0.0	20.575233	2
GCAGAGT	9885	0.0	19.106098	9
TAAGGCG	895	0.0	18.445572	5
AGAGTAC	8035	0.0	17.543669	10-11
GTATTAA	1575	0.0	17.238855	1
TAAGGTG	1195	0.0	16.501112	5
>>END_MODULE