FastQCFastQC Report
Mon 15 Aug 2016
SRR2920896_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920896_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369543
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48950.044.4908261
GGTATCA26600.042.902961
TCAACGC64150.033.745454
ATCAACG64750.033.4327473
CAACGCA65300.032.9271855
AACGCAG68000.031.8994586
TATCAAC68500.031.7382642
GTACATG115100.026.9358041
TACATGG117750.025.7198282
ACGCAGA84850.025.2290387
ACATGGG122200.024.4746443
CGCAGAG89750.023.7966238
GAGTACA87750.020.9711151
CATGGGG79350.020.8199314
AGTACAT85350.020.0219922
GCAGAGT104150.019.3672059
TAAGGCG9700.018.5943685
GTATACG2051.598678E-518.5260624
GGACCGT4700.018.178196
GTATTAA17400.018.0365051
Produced by FastQC (version 0.11.5)